{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,13]],"date-time":"2026-02-13T18:46:57Z","timestamp":1771008417645,"version":"3.50.1"},"reference-count":44,"publisher":"Frontiers Media SA","license":[{"start":{"date-parts":[[2018,12,7]],"date-time":"2018-12-07T00:00:00Z","timestamp":1544140800000},"content-version":"unspecified","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/100000060","name":"National Institute of Allergy and Infectious Diseases","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100000060","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["frontiersin.org"],"crossmark-restriction":true},"short-container-title":["Front. Microbiol."],"DOI":"10.3389\/fmicb.2018.02971","type":"journal-article","created":{"date-parts":[[2018,12,7]],"date-time":"2018-12-07T05:38:53Z","timestamp":1544161133000},"update-policy":"https:\/\/doi.org\/10.3389\/crossmark-policy","source":"Crossref","is-referenced-by-count":9,"title":["PubMLST for Antigen Allele Mining to Inform Development of Gonorrhea Protein-Based Vaccines"],"prefix":"10.3389","volume":"9","author":[{"given":"Benjamin I.","family":"Baarda","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ryszard A.","family":"Zielke","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Robert A.","family":"Nicholas","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Aleksandra E.","family":"Sikora","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1965","published-online":{"date-parts":[[2018,12,7]]},"reference":[{"key":"B1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pmed.1002366","article-title":"Multidrug-resistant gonorrhea: a research and development roadmap to discover new medicines.","volume":"14","author":"Alirol","year":"2017","journal-title":"PLoS Med."},{"key":"B2","doi-asserted-by":"publisher","first-page":"465","DOI":"10.1007\/s12272-009-1400-1","article-title":"Innovative vaccine production technologies: the evolution and value of vaccine production technologies.","volume":"32","author":"Bae","year":"2009","journal-title":"Arch. Pharm. Res."},{"key":"B3","doi-asserted-by":"publisher","first-page":"180","DOI":"10.1126\/science.aad3460","article-title":"The structure of the beta-barrel assembly machinery complex.","volume":"351","author":"Bakelar","year":"2016","journal-title":"Science"},{"key":"B4","year":"2015","journal-title":"Sexually Transmitted Disease Surveillance 2014."},{"key":"B5","year":"2017","journal-title":"Gonorrhea Statistics."},{"key":"B6","year":"2013","journal-title":"Gonorrhea Treatment Guidelines."},{"key":"B7","article-title":"The group B meningococcal vaccine Bexsero induces antibodies that recognize several candidate gonorrhea vaccine targets and shows protective efficacy against experimental Neisseria gonorrhoeae genital tract infection in mice","author":"Connolly","year":"2018","journal-title":"Proceedings of the International Pathogenic Neisseria Conference"},{"key":"B8","doi-asserted-by":"publisher","first-page":"2127","DOI":"10.1099\/00221287-143-7-2127","article-title":"Involvement of the gonococcal MtrE protein in the resistance of Neisseria gonorrhoeae to toxic hydrophobic agents.","volume":"143","author":"Delahay","year":"1997","journal-title":"Microbiology"},{"key":"B9","year":"2012","journal-title":"Response Plan to Control and Manage the Threat of Multidrug-Resistant Gonorrhoea in Europe."},{"key":"B10","doi-asserted-by":"publisher","first-page":"2504","DOI":"10.1056\/NEJMc1512757","article-title":"Failure of dual antimicrobial therapy in treatment of gonorrhea.","volume":"374","author":"Fifer","year":"2016","journal-title":"N. Engl. J. Med."},{"key":"B11","doi-asserted-by":"publisher","first-page":"1018","DOI":"10.1021\/bc100083d","article-title":"Conjugation to nickel-chelating nanolipoprotein particles increases the potency and efficacy of subunit vaccines to prevent West Nile encephalitis.","volume":"21","author":"Fischer","year":"2010","journal-title":"Bioconjug. Chem."},{"key":"B12","doi-asserted-by":"publisher","first-page":"3556","DOI":"10.1128\/AAC.00038-14","article-title":"Importance of multidrug efflux pumps in the antimicrobial resistance property of clinical multidrug-resistant isolates of Neisseria gonorrhoeae.","volume":"58","author":"Golparian","year":"2014","journal-title":"Antimicrob. Agents Chemother."},{"key":"B13","doi-asserted-by":"publisher","first-page":"1579","DOI":"10.1016\/j.vaccine.2013.08.067","article-title":"Vaccines against gonorrhea: current status and future challenges.","volume":"32","author":"Jerse","year":"2014","journal-title":"Vaccine"},{"key":"B14","doi-asserted-by":"publisher","first-page":"iv63","DOI":"10.1136\/sextrans-2013-051225","article-title":"Vaccine research for gonococcal infections: where are we?","volume":"89","author":"Jerse","year":"2013","journal-title":"Sex. Transm. Infect."},{"key":"B15","doi-asserted-by":"publisher","first-page":"5576","DOI":"10.1128\/IAI.71.10.5576-5582.2003","article-title":"A gonococcal efflux pump system enhances bacterial survival in a female mouse model of genital tract infection.","volume":"71","author":"Jerse","year":"2003","journal-title":"Infect. Immun."},{"key":"B16","doi-asserted-by":"publisher","DOI":"10.12688\/wellcomeopenres.14826.1","article-title":"Open-access bacterial population genomics: BIGSdb software, the PubMLST.org website and their applications.","volume":"3","author":"Jolley","year":"2018","journal-title":"Wellcome Open Res."},{"key":"B17","doi-asserted-by":"publisher","DOI":"10.1186\/1471-2105-5-86","article-title":"mlstdbNet - distributed multi-locus sequence typing (MLST) databases.","volume":"5","author":"Jolley","year":"2004","journal-title":"BMC Bioinformatics"},{"key":"B18","doi-asserted-by":"publisher","DOI":"10.1186\/1471-2105-11-595","article-title":"BIGSdb: scalable analysis of bacterial genome variation at the population level.","volume":"11","author":"Jolley","year":"2010","journal-title":"BMC Bioinformatics"},{"key":"B19","doi-asserted-by":"publisher","first-page":"206","DOI":"10.1038\/nrmicro2069","article-title":"Membrane protein architects: the role of the BAM complex in outer membrane protein assembly.","volume":"7","author":"Knowles","year":"2009","journal-title":"Nat. Rev. Microbiol."},{"key":"B20","doi-asserted-by":"publisher","first-page":"1870","DOI":"10.1093\/molbev\/msw054","article-title":"MEGA7: molecular evolutionary genetics analysis version 7.0 for bigger datasets.","volume":"33","author":"Kumar","year":"2016","journal-title":"Mol. Biol. Evol."},{"key":"B21","doi-asserted-by":"publisher","first-page":"839","DOI":"10.1046\/j.1365-2958.1999.01530.x","article-title":"The farAB-encoded efflux pump mediates resistance of gonococci to long-chained antibacterial fatty acids.","volume":"33","author":"Lee","year":"1999","journal-title":"Mol. Microbiol."},{"key":"B22","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0097475","article-title":"Crystal structure of the open state of the Neisseria gonorrhoeae MtrE outer membrane channel.","volume":"9","author":"Lei","year":"2014","journal-title":"PLoS One"},{"key":"B23","doi-asserted-by":"publisher","first-page":"3140","DOI":"10.1073\/pnas.95.6.3140","article-title":"Multilocus sequence typing: a portable approach to the identification of clones within populations of pathogenic microorganisms.","volume":"95","author":"Maiden","year":"1998","journal-title":"Proc. Natl. Acad. Sci. U.S.A."},{"key":"B24","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0143304","article-title":"Global estimates of the prevalence and incidence of four curable sexually transmitted infections in 2012 based on systematic review and global reporting.","volume":"10","author":"Newman","year":"2015","journal-title":"PLoS One"},{"key":"B25","doi-asserted-by":"publisher","first-page":"197","DOI":"10.1038\/nrmicro.2016.191","article-title":"The beta-barrel assembly machinery in motion.","volume":"15","author":"Noinaj","year":"2017","journal-title":"Nat. Rev. Microbiol."},{"key":"B26","doi-asserted-by":"publisher","first-page":"385","DOI":"10.1038\/nature12521","article-title":"Structural insight into the biogenesis of beta-barrel membrane proteins.","volume":"501","author":"Noinaj","year":"2013","journal-title":"Nature"},{"key":"B27","doi-asserted-by":"publisher","first-page":"6303","DOI":"10.1021\/acs.biochem.5b00852","article-title":"Fitting the pieces of the beta-barrel assembly machinery complex.","volume":"54","author":"O\u2019Neil","year":"2015","journal-title":"Biochemistry"},{"key":"B28","doi-asserted-by":"publisher","first-page":"1603","DOI":"10.1016\/S0140-6736(17)31449-6","article-title":"Effectiveness of a group B outer membrane vesicle meningococcal vaccine against gonorrhoea in New Zealand: a retrospective case-control study.","volume":"390","author":"Petousis-Harris","year":"2017","journal-title":"Lancet"},{"key":"B29","first-page":"1","article-title":"UK case of Neisseria gonorrhoeae with high-level resistance to azithromycin and resistance to ceftriaxone acquired abroad.","volume":"12","year":"2018","journal-title":"Health Protect. Rep."},{"key":"B30","doi-asserted-by":"publisher","first-page":"665","DOI":"10.1146\/annurev-micro-090816-093530","article-title":"Neisseria gonorrhoeae: drug resistance, mouse models, and vaccine development.","volume":"71","author":"Rice","year":"2017","journal-title":"Annu. Rev. Microbiol."},{"key":"B31","doi-asserted-by":"publisher","first-page":"856","DOI":"10.1093\/jac\/dki333","article-title":"Characterization of the MacA-MacB efflux system in Neisseria gonorrhoeae.","volume":"56","author":"Rouquette-Loughlin","year":"2005","journal-title":"J. Antimicrob. Chemother."},{"key":"B32","doi-asserted-by":"publisher","first-page":"1106","DOI":"10.1074\/jbc.RA117.000437","article-title":"Structural and functional insights into the role of BamD and BamE within the beta-barrel assembly machinery in Neisseria gonorrhoeae.","volume":"293","author":"Sikora","year":"2018","journal-title":"J. Biol. Chem."},{"key":"B33","doi-asserted-by":"publisher","first-page":"587","DOI":"10.1128\/CMR.00010-14","article-title":"Antimicrobial resistance in Neisseria gonorrhoeae in the 21st century: past, evolution, and future.","volume":"27","author":"Unemo","year":"2014","journal-title":"Clin. Microbiol. Rev."},{"key":"B34","doi-asserted-by":"publisher","first-page":"643","DOI":"10.1038\/nrurol.2017.139","article-title":"Infection: proof of principle for effectiveness of a gonorrhoea vaccine.","volume":"14","author":"Unemo","year":"2017","journal-title":"Nat. Rev. Urol."},{"key":"B35","doi-asserted-by":"publisher","first-page":"5619","DOI":"10.1128\/JB.184.20.5619-5624.2002","article-title":"Overexpression of the MtrC-MtrD-MtrE efflux pump due to an mtrR mutation is required for chromosomally mediated penicillin resistance in Neisseria gonorrhoeae.","volume":"184","author":"Veal","year":"2002","journal-title":"J. Bacteriol."},{"key":"B36","doi-asserted-by":"publisher","first-page":"7074","DOI":"10.1128\/JB.00737-09","article-title":"The beta-barrel outer membrane protein assembly complex of Neisseria meningitidis.","volume":"191","author":"Volokhina","year":"2009","journal-title":"J. Bacteriol."},{"key":"B37","article-title":"Prevalence and incidence of selected sexually transmitted infections. Chlamydia trachomatis, Neisseria gonorrhoeae, syphilis and Trichomonas vaginalis","year":"2011","journal-title":"Proceedings of the Methods and Results used by WHO to Generate 2005 Estimates"},{"key":"B38","year":"2012","journal-title":"Global Action Plan to Control the Spread and Impact of Antimicrobial Resistance in Neisseria gonorrhoeae."},{"key":"B39","year":"2015","journal-title":"Antimicrobial Resistance Draft Global Action Plan on Antimicrobial Resistance."},{"key":"B40","year":"2016","journal-title":"WHO | Global Health Sector Strategy on Sexually Transmitted Infections, 2016-2021."},{"key":"B41","doi-asserted-by":"publisher","DOI":"10.3389\/fmicb.2011.00124","article-title":"Vaccines for gonorrhea: can we rise to the challenge?","volume":"2","author":"Zhu","year":"2011","journal-title":"Front. Microbiol."},{"key":"B42","doi-asserted-by":"publisher","DOI":"10.1371\/journal.ppat.1007081","article-title":"SliC is a surface-displayed lipoprotein that is required for the anti-lysozyme strategy during Neisseria gonorrhoeae infection.","volume":"14","author":"Zielke","year":"2018","journal-title":"PLoS Pathog."},{"key":"B43","doi-asserted-by":"publisher","first-page":"2338","DOI":"10.1074\/mcp.M116.058800","article-title":"Proteomics-driven antigen discovery for development of vaccines against gonorrhea.","volume":"15","author":"Zielke","year":"2016","journal-title":"Mol. Cell. Proteomics"},{"key":"B44","doi-asserted-by":"publisher","first-page":"1299","DOI":"10.1074\/mcp.M113.029538","article-title":"Quantitative proteomics of the Neisseria gonorrhoeae cell envelope and membrane vesicles for the discovery of potential therapeutic targets.","volume":"13","author":"Zielke","year":"2014","journal-title":"Mol. Cell. Proteomics"}],"container-title":["Frontiers in Microbiology"],"original-title":[],"link":[{"URL":"https:\/\/www.frontiersin.org\/article\/10.3389\/fmicb.2018.02971\/full","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2018,12,7]],"date-time":"2018-12-07T05:38:56Z","timestamp":1544161136000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.frontiersin.org\/article\/10.3389\/fmicb.2018.02971\/full"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2018,12,7]]},"references-count":44,"alternative-id":["10.3389\/fmicb.2018.02971"],"URL":"https:\/\/doi.org\/10.3389\/fmicb.2018.02971","relation":{},"ISSN":["1664-302X"],"issn-type":[{"value":"1664-302X","type":"electronic"}],"subject":[],"published":{"date-parts":[[2018,12,7]]},"article-number":"2971"}}