{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,28]],"date-time":"2026-09-28T21:09:13Z","timestamp":1790629753760,"version":"4.1.0"},"reference-count":45,"publisher":"Frontiers Media SA","license":[{"start":{"date-parts":[[2018,12,18]],"date-time":"2018-12-18T00:00:00Z","timestamp":1545091200000},"content-version":"unspecified","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["frontiersin.org"],"crossmark-restriction":true},"short-container-title":["Front. Microbiol."],"DOI":"10.3389\/fmicb.2018.03151","type":"journal-article","created":{"date-parts":[[2018,12,18]],"date-time":"2018-12-18T07:51:58Z","timestamp":1545119518000},"update-policy":"https:\/\/doi.org\/10.3389\/crossmark-policy","source":"Crossref","is-referenced-by-count":9,"title":["Red Fluorescent Chlamydia trachomatis Applied to Live Cell Imaging and Screening for Antibacterial Agents"],"prefix":"10.3389","volume":"9","author":[{"given":"Sergio A.","family":"Mojica","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Anna U.","family":"Eriksson","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rohan A.","family":"Davis","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wael","family":"Bahnan","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mikael","family":"Elofsson","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"\u00c5sa","family":"Gylfe","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1965","published-online":{"date-parts":[[2018,12,18]]},"reference":[{"key":"B1","doi-asserted-by":"publisher","first-page":"587","DOI":"10.1016\/j.febslet.2007.01.013","article-title":"Small molecule inhibitors of type III secretion in Yersinia block the Chlamydia pneumoniae infection cycle","volume":"581","author":"Bailey","year":"2007","journal-title":"FEBS Lett."},{"key":"B2","doi-asserted-by":"publisher","first-page":"2989","DOI":"10.1128\/JB.01677-14","article-title":"Benzylidene acylhydrazides inhibit chlamydial growth in a type III secretion- and iron chelation-independent manner","volume":"196","author":"Bao","year":"2014","journal-title":"J. Bacteriol."},{"key":"B3","doi-asserted-by":"publisher","first-page":"372","DOI":"10.1039\/c5np00121h","article-title":"The use of isolated natural products as scaffolds for the generation of chemically diverse screening libraries for drug discovery","volume":"33","author":"Barnes","year":"2016","journal-title":"Nat. Prod. Rep."},{"key":"B4","doi-asserted-by":"publisher","first-page":"283","DOI":"10.1016\/j.tet.2009.10.109","article-title":"Ecionines A and B, two new cytotoxic pyridoacridine alkaloids from the Australian marine sponge, Ecionemia geodides","volume":"66","author":"Barnes","year":"2010","journal-title":"Tetrahedron"},{"key":"B5","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1128\/9781555817329.ch1","article-title":"Chlamydia infection and epidemiology","volume-title":"Intracellular Pathogens I: Chlamydiales","author":"Batteiger","year":"2012"},{"key":"B6","doi-asserted-by":"publisher","first-page":"1325","DOI":"10.1128\/jb.01290-13","article-title":"Expression and targeting of secreted proteins from Chlamydia trachomatis","volume":"196","author":"Bauler","year":"2014","journal-title":"J. Bacteriol."},{"key":"B7","doi-asserted-by":"publisher","first-page":"79","DOI":"10.1016\/j.phytochem.2015.12.014","article-title":"Antibacterial and antifungal screening of natural products sourced from Australian fungi and characterisation of pestalactams D\u2013F","volume":"124","author":"Beattie","year":"2016","journal-title":"Phytochemistry"},{"key":"B8","doi-asserted-by":"publisher","first-page":"75","DOI":"10.1186\/s12866-017-0982-x","article-title":"Short-term increase in prevalence of nasopharyngeal carriage of macrolide-resistant Staphylococcus aureus following mass drug administration with azithromycin for trachoma control","volume":"17","author":"Bojang","year":"2017","journal-title":"BMC Microbiol."},{"key":"B9","doi-asserted-by":"crossref","first-page":"1161","DOI":"10.1128\/IAI.31.3.1161-1176.1981","article-title":"Purification and partial characterization of the major outer membrane protein of Chlamydia trachomatis","volume":"31","author":"Caldwell","year":"1981","journal-title":"Infect. Immun."},{"key":"B10","doi-asserted-by":"publisher","first-page":"7167","DOI":"10.1016\/j.bmc.2012.09.052","article-title":"Synthesis and antimalarial evaluation of a screening library based on a tetrahydroanthraquinone natural product scaffold","volume":"20","author":"Choomuenwai","year":"2012","journal-title":"Bioorg. Med. Chem."},{"key":"B11","doi-asserted-by":"publisher","first-page":"2686","DOI":"10.1016\/j.bmc.2010.02.022","article-title":"Statistical molecular design of a focused salicylidene acylhydrazide library and multivariate QSAR of inhibition of type III secretion in the Gram-negative bacterium Yersinia","volume":"18","author":"Dahlgren","year":"2010","journal-title":"Bioorg. Med. Chem."},{"key":"B12","doi-asserted-by":"publisher","first-page":"769","DOI":"10.1021\/np050025h","article-title":"Isolation and structure elucidation of the new fungal metabolite (-)-xylariamide A","volume":"68","author":"Davis","year":"2005","journal-title":"J. Nat. Prod."},{"key":"B13","doi-asserted-by":"publisher","first-page":"9608","DOI":"10.1021\/jo400988y","article-title":"Thiaplakortones A-D: antimalarial thiazine alkaloids from the Australian marine sponge Plakortis lita","volume":"78","author":"Davis","year":"2013","journal-title":"J. Org. Chem."},{"key":"B14","doi-asserted-by":"crossref","first-page":"1115","DOI":"10.1071\/CH99141","article-title":"Pigments of fungi. LXII. (1S, 3R)-austrocortirubin: isolation from the fungus Dermocybe splendida and synthesis from (S)-citramalic acid","volume":"52","author":"Elsworth","year":"1999","journal-title":"Aust. J. Chem."},{"key":"B15","doi-asserted-by":"publisher","first-page":"e02304","DOI":"10.1128\/mBio.02304-14","article-title":"A 2-pyridone-amide inhibitor targets the glucose metabolism pathway of Chlamydia trachomatis","volume":"6","author":"Engstr\u00f6m","year":"2014","journal-title":"mBio"},{"key":"B16","doi-asserted-by":"publisher","first-page":"3550","DOI":"10.1016\/j.bmcl.2012.03.096","article-title":"Derivatives of 8-hydroxyquinoline\u2013antibacterial agents that target intra- and extracellular Gram-negative pathogens","volume":"22","author":"Enquist","year":"2012","journal-title":"Bioorg. Med. Chem. Lett."},{"key":"B17","doi-asserted-by":"publisher","first-page":"136","DOI":"10.1016\/j.plantsci.2015.03.010","article-title":"Autofluorescence: biological functions and technical applications","volume":"236","author":"Garc\u00efa-Plazaola","year":"2015","journal-title":"Plant. Sci."},{"key":"B18","doi-asserted-by":"publisher","first-page":"676","DOI":"10.1111\/mmi.12306","article-title":"Identification of a serine protease inhibitor which causes inclusion vacuole reduction and is lethal to Chlamydia trachomatis","volume":"89","author":"Gloeckl","year":"2013","journal-title":"Mol. Microbiol."},{"key":"B19","doi-asserted-by":"publisher","first-page":"2094","DOI":"10.1021\/acs.jmedchem.5b01759","article-title":"Thiazolino 2-pyridone amide inhibitors of Chlamydia trachomatis infectivity","volume":"59","author":"Good","year":"2016","journal-title":"J. Med. Chem."},{"key":"B20","doi-asserted-by":"publisher","first-page":"9393","DOI":"10.1021\/acs.jmedchem.7b00716","article-title":"Thiazolino 2-pyridone amide isosteres as inhibitors of Chlamydia trachomatis infectivity","volume":"60","author":"Good","year":"2017","journal-title":"J. Med. Chem."},{"key":"B21","doi-asserted-by":"publisher","first-page":"609","DOI":"10.1038\/ja.2015.48","article-title":"Dibenzocyclooctadiene lignans from Schisandra spp. selectively inhibit the growth of the intracellular bacteria Chlamydia pneumoniae and Chlamydia trachomatis","volume":"68","author":"Hakala","year":"2015","journal-title":"J. Antibiot."},{"key":"B22","doi-asserted-by":"publisher","first-page":"545","DOI":"10.1517\/14656566.2012.658776","article-title":"Treatment of chlamydial infections","volume":"13","author":"Hammerschlag","year":"2012","journal-title":"Expert Opin. Pharmacother."},{"key":"B23","doi-asserted-by":"publisher","first-page":"e115115","DOI":"10.1371\/journal.pone.0115115","article-title":"Inhibitory activity of the isoflavone biochanin A on intracellular bacteria of genus Chlamydia and initial development of a buccal formulation","volume":"9","author":"Hanski","year":"2014","journal-title":"PLoS ONE"},{"key":"B24","doi-asserted-by":"publisher","first-page":"73","DOI":"10.1097\/QCO.0b013e32834e9a6a","article-title":"Antimicrobial resistance in sexually transmitted infections in the developed world: implications for rational treatment","volume":"25","author":"Ison","year":"2012","journal-title":"Curr. Opin. Infect. Dis."},{"key":"B25","doi-asserted-by":"publisher","first-page":"121","DOI":"10.1016\/j.phytochem.2012.09.019","article-title":"Pyridocoumarin, aristolactam and aporphine alkaloids from the Australian rainforest plant Goniothalamus australis","volume":"86","author":"Levrier","year":"2013","journal-title":"Phytochemistry"},{"key":"B26","doi-asserted-by":"publisher","first-page":"161","DOI":"10.1126\/science.1168243","article-title":"Drug discovery and natural products: end of an era or an endless frontier?","volume":"325","author":"Li","year":"2009","journal-title":"Science"},{"key":"B27","doi-asserted-by":"publisher","first-page":"2968","DOI":"10.1128\/aac.02015-13","article-title":"N-acylated derivatives of sulfamethoxazole and sulfafurazole inhibit intracellular growth of Chlamydia trachomatis","volume":"58","author":"Marwaha","year":"2014","journal-title":"Antimicrob. Agents Chemother."},{"key":"B28","doi-asserted-by":"publisher","first-page":"14566","DOI":"10.1073\/pnas.0606412103","article-title":"A small-molecule inhibitor of type III secretion inhibits different stages of the infectious cycle of Chlamydia trachomatis","volume":"103","author":"Muschiol","year":"2006","journal-title":"Proc. Natl. Acad. Sci. U.S.A"},{"key":"B29","doi-asserted-by":"publisher","first-page":"e0143304","DOI":"10.1371\/journal.pone.0143304","article-title":"Global estimates of the prevalence and incidence of four curable sexually transmitted infections in 2012 based on systematic review and global reporting","volume":"10","author":"Newman","year":"2015","journal-title":"PLoS ONE"},{"key":"B30","doi-asserted-by":"publisher","first-page":"738","DOI":"10.1016\/j.micinf.2015.09.004","article-title":"In vitro susceptibility of recent Chlamydia trachomatis clinical isolates to the CtHtrA inhibitor JO146","volume":"17","author":"Ong","year":"2015","journal-title":"Microbes Infect."},{"key":"B31","doi-asserted-by":"publisher","first-page":"2838","DOI":"10.1128\/AAC.00056-13","article-title":"A simple resazurin-based microplate assay for measuring Chlamydia infections","volume":"57","author":"Osaka","year":"2013","journal-title":"Antimicrob Agents Chemother"},{"key":"B32","doi-asserted-by":"publisher","first-page":"4184","DOI":"10.1128\/AAC.00427-12","article-title":"An automated image-based method for rapid analysis of Chlamydia infection as a tool for screening antichlamydial agents","volume":"56","author":"Osaka","year":"2012","journal-title":"Antimicrob. Agents Chemother."},{"key":"B33","doi-asserted-by":"publisher","first-page":"111","DOI":"10.1016\/j.tibs.2016.09.010","article-title":"The growing and glowing toolbox of fluorescent and photoactive proteins","volume":"42","author":"Rodriguez","year":"2017","journal-title":"Trends Biochem. Sci."},{"key":"B34","doi-asserted-by":"publisher","first-page":"1077","DOI":"10.1016\/j.ejmech.2017.11.099","article-title":"Natural product inspired library synthesis\u2014Identification of 2,3-diarylbenzofuran and 2,3-dihydrobenzofuran based inhibitors of Chlamydia trachomatis","volume":"143","author":"Saleeb","year":"2018","journal-title":"Eur. J. Med. Chem."},{"key":"B35","doi-asserted-by":"publisher","first-page":"4296","DOI":"10.1128\/AAC.00356-12","article-title":"Resistance to a novel antichlamydial compound is mediated through mutations in Chlamydia trachomatis secY","volume":"56","author":"Sandoz","year":"2012","journal-title":"Antimicrob. Agents Chemother."},{"key":"B36","doi-asserted-by":"publisher","first-page":"1558","DOI":"10.1039\/c4ob01849d","article-title":"Synthesis and antimalarial evaluation of amide and urea derivatives based on the thiaplakortone A natural product scaffold","volume":"13","author":"Schwartz","year":"2015","journal-title":"Org. Biomol. Chem."},{"key":"B37","doi-asserted-by":"crossref","first-page":"389","DOI":"10.1128\/CMR.8.3.389","article-title":"Laboratory-associated infections and biosafety","volume":"8","author":"Sewell","year":"1995","journal-title":"Clin. Microbiol. Rev."},{"key":"B38","doi-asserted-by":"publisher","first-page":"9639","DOI":"10.1021\/bi060773l","article-title":"Novel chromophores and buried charges control color in mFruits","volume":"45","author":"Shu","year":"2006","journal-title":"Biochemistry"},{"key":"B39","doi-asserted-by":"publisher","first-page":"595","DOI":"10.1016\/j.ejmech.2015.07.019","article-title":"Design, synthesis and evaluation of novel polypharmacological antichlamydial agents","volume":"101","author":"Sunduru","year":"2015","journal-title":"Eur. J. Med. Chem."},{"key":"B40","doi-asserted-by":"publisher","first-page":"397","DOI":"10.1038\/ja.2012.43","article-title":"Pre-clinical pharmacokinetics and anti-chlamydial activity of salicylidene acylhydrazide inhibitors of bacterial type III secretion","volume":"65","author":"Ur-Rehman","year":"2012","journal-title":"J. Antibiot."},{"key":"B41","doi-asserted-by":"publisher","first-page":"249","DOI":"10.1016\/j.bmcl.2014.11.055","article-title":"The fungal natural product (1S,3S)-austrocortirubin induces DNA damage in HCT116 cells via a mechanism unique from other DNA damaging agents","volume":"25","author":"Wang","year":"2015","journal-title":"Bioorg. Med. Chem. Lett."},{"key":"B42","doi-asserted-by":"publisher","first-page":"e1002258","DOI":"10.1371\/journal.ppat.1002258","article-title":"Development of a transformation system for Chlamydia trachomatis: restoration of glycogen biosynthesis by acquisition of a plasmid shuttle vector","volume":"7","author":"Wang","year":"2011","journal-title":"PLoS Pathog."},{"key":"B43","volume-title":"Trachoma: Fact Sheet","year":"2018"},{"key":"B44","doi-asserted-by":"publisher","first-page":"1543","DOI":"10.1111\/j.1365-2958.2006.05347.x","article-title":"Treatment of Chlamydia trachomatis with a small molecule inhibitor of the Yersinia type III secretion system disrupts progression of the chlamydial developmental cycle","volume":"61","author":"Wolf","year":"2006","journal-title":"Mol. Microbiol."},{"key":"B45","doi-asserted-by":"publisher","first-page":"E1715","DOI":"10.3390\/molecules22101715","article-title":"Screening a natural product-based library against kinetoplastid parasites","volume":"22","author":"Zulfiqar","year":"2017","journal-title":"Molecules"}],"container-title":["Frontiers in Microbiology"],"original-title":[],"link":[{"URL":"https:\/\/www.frontiersin.org\/article\/10.3389\/fmicb.2018.03151\/full","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2020,11,18]],"date-time":"2020-11-18T02:37:08Z","timestamp":1605667028000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.frontiersin.org\/article\/10.3389\/fmicb.2018.03151\/full"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2018,12,18]]},"references-count":45,"alternative-id":["10.3389\/fmicb.2018.03151"],"URL":"https:\/\/doi.org\/10.3389\/fmicb.2018.03151","relation":{},"ISSN":["1664-302X"],"issn-type":[{"value":"1664-302X","type":"electronic"}],"subject":[],"published":{"date-parts":[[2018,12,18]]},"article-number":"3151"}}