{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,3]],"date-time":"2026-09-03T21:07:10Z","timestamp":1788469630320,"version":"build-2803163510"},"reference-count":38,"publisher":"Frontiers Media SA","license":[{"start":{"date-parts":[[2019,4,9]],"date-time":"2019-04-09T00:00:00Z","timestamp":1554768000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["81702028"],"award-info":[{"award-number":["81702028"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["frontiersin.org"],"crossmark-restriction":true},"short-container-title":["Front. Microbiol."],"DOI":"10.3389\/fmicb.2019.00734","type":"journal-article","created":{"date-parts":[[2019,4,9]],"date-time":"2019-04-09T06:15:03Z","timestamp":1554790503000},"update-policy":"https:\/\/doi.org\/10.3389\/crossmark-policy","source":"Crossref","is-referenced-by-count":22,"title":["The Virulence-Related MYR1 Protein of Toxoplasma gondii as a Novel DNA Vaccine Against Toxoplasmosis in Mice"],"prefix":"10.3389","volume":"10","author":[{"given":"Bin","family":"Zheng","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jianzu","family":"Ding","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Di","family":"Lou","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Qunbo","family":"Tong","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xunhui","family":"Zhuo","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Haojie","family":"Ding","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Qingming","family":"Kong","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shaohong","family":"Lu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1965","published-online":{"date-parts":[[2019,4,9]]},"reference":[{"key":"B1","doi-asserted-by":"publisher","first-page":"677","DOI":"10.1016\/j.biopha.2016.05.041","article-title":"Human toxoplasmosis-searching for novel chemotherapeutics.","volume":"82","author":"Antczak","year":"2016","journal-title":"Biomed. Pharmacother."},{"key":"B2","doi-asserted-by":"publisher","first-page":"1750","DOI":"10.1016\/j.vaccine.2009.01.022","article-title":"Exosomes are an effective vaccine against congenital toxoplasmosis in mice.","volume":"27","author":"Beauvillain","year":"2009","journal-title":"Vaccine"},{"key":"B3","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0153856","article-title":"Investigating the determinants of Toxoplasma gondii prevalence in meat: a systematic review and meta-regression.","volume":"11","author":"Belluco","year":"2016","journal-title":"PLoS One"},{"key":"B4","doi-asserted-by":"publisher","first-page":"463","DOI":"10.1146\/annurev-micro-091014-104100","article-title":"Lytic cycle of Toxoplasma gondii: 15 years later.","volume":"69","author":"Blader","year":"2015","journal-title":"Annu. Rev. Microbiol."},{"key":"B5","doi-asserted-by":"publisher","DOI":"10.3389\/fmicb.2016.00609","article-title":"Evaluation of immunoprotection conferred by the subunit vaccines of GRA2 and GRA5 against acute toxoplasmosis in BALB\/c mice.","volume":"7","author":"Ching","year":"2016","journal-title":"Front. Microbiol."},{"key":"B6","doi-asserted-by":"publisher","first-page":"467","DOI":"10.1111\/j.1550-7408.2008.00345.x","article-title":"The history of Toxoplasma gondii\u2013the first 100 years.","volume":"55","author":"Dubey","year":"2008","journal-title":"J. Eukaryot. Microbiol."},{"key":"B7","doi-asserted-by":"publisher","first-page":"793","DOI":"10.1007\/s00281-012-0339-3","article-title":"Immune response and immunopathology during toxoplasmosis.","volume":"34","author":"Dupont","year":"2012","journal-title":"Semin. Immunopathol."},{"key":"B8","doi-asserted-by":"publisher","DOI":"10.1128\/mBio.02231-15","article-title":"A novel secreted protein, MYR1, is central to toxoplasma\u2019s manipulation of host cells.","volume":"7","author":"Franco","year":"2016","journal-title":"mBio"},{"key":"B9","doi-asserted-by":"publisher","first-page":"766","DOI":"10.1038\/nrmicro2858","article-title":"Modulation of innate immunity by Toxoplasma gondii virulence effectors.","volume":"10","author":"Hunter","year":"2012","journal-title":"Nat. Rev. Microbiol."},{"key":"B10","doi-asserted-by":"publisher","first-page":"472","DOI":"10.1016\/j.chom.2011.04.015","article-title":"Toxoplasma polymorphic effectors determine macrophage polarization and intestinal inflammation.","volume":"9","author":"Jensen","year":"2011","journal-title":"Cell Host. Microbe"},{"key":"B11","doi-asserted-by":"publisher","first-page":"1113","DOI":"10.4049\/jimmunol.179.2.1113","article-title":"The antiviral CD8+ T cell response is differentially dependent on CD4+ T cell help over the course of persistent infection.","volume":"179","author":"Kemball","year":"2007","journal-title":"J. Immunol."},{"key":"B12","doi-asserted-by":"publisher","DOI":"10.3389\/fimmu.2018.00317","article-title":"Targeted delivery of Toxoplasma gondii antigens to dendritic cells promote immunogenicity and protective efficiency against toxoplasmosis.","volume":"9","author":"Lakhrif","year":"2018","journal-title":"Front. Immunol."},{"key":"B13","doi-asserted-by":"publisher","first-page":"5692","DOI":"10.1016\/j.vaccine.2018.08.016","article-title":"Virus-like particle vaccines expressing Toxoplasma gondii rhoptry protein 18 and microneme protein 8 provide enhanced protection.","volume":"36","author":"Lee","year":"2018","journal-title":"Vaccine"},{"key":"B14","doi-asserted-by":"publisher","first-page":"467","DOI":"10.2147\/IJN.S151110","article-title":"Characterization of exosomes derived from Toxoplasma gondii and their functions in modulating immune responses.","volume":"13","author":"Li","year":"2018","journal-title":"Int. J. Nanomed."},{"key":"B15","doi-asserted-by":"publisher","first-page":"273","DOI":"10.1080\/14712598.2018.1413086","article-title":"Moving towards improved vaccines for Toxoplasma gondii.","volume":"18","author":"Li","year":"2018","journal-title":"Expert Opin. Biol. Ther."},{"key":"B16","doi-asserted-by":"publisher","first-page":"4047","DOI":"10.1128\/IAI.01615-14","article-title":"Type II Toxoplasma gondii induction of CD40 on infected macrophages enhances interleukin-12 responses.","volume":"82","author":"Morgado","year":"2014","journal-title":"Infect. Immun."},{"key":"B17","doi-asserted-by":"publisher","first-page":"1385","DOI":"10.1016\/j.ijpara.2009.04.003","article-title":"Toxoplasmosis snapshots: global status of Toxoplasma gondii seroprevalence and implications for pregnancy and congenital toxoplasmosis.","volume":"39","author":"Pappas","year":"2009","journal-title":"Int. J. Parasitol."},{"key":"B18","doi-asserted-by":"publisher","first-page":"388","DOI":"10.1016\/j.pt.2011.03.009","article-title":"Innate responses to Toxoplasma gondii in mice and humans.","volume":"27","author":"Pifer","year":"2011","journal-title":"Trends Parasitol."},{"key":"B19","doi-asserted-by":"publisher","DOI":"10.3389\/fmicb.2016.00808","article-title":"Induction of protective immunity against toxoplasmosis in BALB\/c mice vaccinated with Toxoplasma gondii rhoptry-1.","volume":"7","author":"Sonaimuthu","year":"2016","journal-title":"Front. Microbiol."},{"key":"B20","doi-asserted-by":"publisher","first-page":"3090","DOI":"10.1128\/IAI.01722-14","article-title":"Complex immune cell interplay in the gamma interferon response during Toxoplasma gondii infection.","volume":"82","author":"Sturge","year":"2014","journal-title":"Infect. Immun."},{"key":"B21","doi-asserted-by":"publisher","first-page":"717","DOI":"10.1111\/j.1574-6976.2011.00305.x","article-title":"Mechanisms of Toxoplasma gondii persistence and latency.","volume":"36","author":"Sullivan","year":"2012","journal-title":"FEMS Microbiol. Rev."},{"key":"B22","doi-asserted-by":"publisher","first-page":"501","DOI":"10.2471\/BLT.12.111732","article-title":"The global burden of congenital toxoplasmosis: a systematic review.","volume":"91","author":"Torgerson","year":"2013","journal-title":"Bull. World Health Organ."},{"key":"B23","doi-asserted-by":"publisher","DOI":"10.3389\/fimmu.2017.00730","article-title":"Immunization with Toxoplasma gondii GRA17 deletion mutant induces partial protection and survival in challenged mice.","volume":"8","author":"Wang","year":"2017","journal-title":"Front. Immunol."},{"key":"B24","doi-asserted-by":"publisher","DOI":"10.3389\/fmicb.2017.00168","article-title":"Toxoplasma gondii elongation factor 1-alpha (TgEF-1alpha) is a novel vaccine candidate antigen against toxoplasmosis.","volume":"8","author":"Wang","year":"2017","journal-title":"Front. Microbiol."},{"key":"B25","doi-asserted-by":"publisher","first-page":"768","DOI":"10.1093\/infdis\/jiy211","article-title":"Live attenuated Pru: deltacdpk2 strain of Toxoplasma gondii protects against acute, chronic, and congenital toxoplasmosis.","volume":"218","author":"Wang","year":"2018","journal-title":"J. Infect. Dis."},{"key":"B26","doi-asserted-by":"publisher","first-page":"677","DOI":"10.1016\/j.transproceed.2012.02.036","article-title":"The soluble tachyzoite antigen of Toxoplasma gondii has a protective effect on mouse allografts.","volume":"45","author":"Wang","year":"2013","journal-title":"Trans. Proc."},{"key":"B27","doi-asserted-by":"publisher","first-page":"466","DOI":"10.1111\/cmi.12267","article-title":"Toxoplasma development - turn the switch on or off?","volume":"16","author":"White","year":"2014","journal-title":"Cell Microbiol."},{"key":"B28","doi-asserted-by":"publisher","DOI":"10.3389\/fimmu.2018.01814","article-title":"A lactate fermentation mutant of toxoplasma stimulates protective immunity against acute and chronic toxoplasmosis.","volume":"9","author":"Xia","year":"2018","journal-title":"Front. Immunol."},{"key":"B29","doi-asserted-by":"publisher","first-page":"109","DOI":"10.1038\/nri3598","article-title":"Innate immunity to Toxoplasma gondii infection.","volume":"14","author":"Yarovinsky","year":"2014","journal-title":"Nat. Rev. Immunol."},{"key":"B30","doi-asserted-by":"publisher","DOI":"10.3389\/fimmu.2017.01703","article-title":"Macrophage-microbe interactions: lessons from the zebrafish model.","volume":"8","author":"Yoshida","year":"2017","journal-title":"Front. Immunol."},{"key":"B31","doi-asserted-by":"publisher","DOI":"10.3389\/fimmu.2018.01505","article-title":"Immunization with a DNA vaccine cocktail encoding TgPF, TgROP16, TgROP18, TgMIC6, and TgCDPK3 genes protects mice against chronic toxoplasmosis.","volume":"9","author":"Zhang","year":"2018","journal-title":"Front. Immunol."},{"key":"B32","doi-asserted-by":"publisher","DOI":"10.3389\/fmicb.2018.00909","article-title":"Immune protection of rhoptry protein 21 (ROP21) of Toxoplasma gondii as a DNA vaccine against toxoplasmosis.","volume":"9","author":"Zhang","year":"2018","journal-title":"Front. Microbiol."},{"key":"B33","doi-asserted-by":"publisher","first-page":"1289","DOI":"10.1586\/14760584.2015.1061938","article-title":"Protective efficacy of two novel DNA vaccines expressing Toxoplasma gondii rhomboid 4 and rhomboid 5 proteins against acute and chronic toxoplasmosis in mice.","volume":"14","author":"Zhang","year":"2015","journal-title":"Expert Rev. Vaccines"},{"key":"B34","doi-asserted-by":"publisher","DOI":"10.3389\/fmicb.2017.00216","article-title":"Immuno-efficacy of a T. gondii secreted protein with an altered thrombospondin repeat (TgSPATR) as a novel DNA vaccine candidate against acute toxoplasmosis in BALB\/c mice.","volume":"8","author":"Zheng","year":"2017","journal-title":"Front. Microbiol."},{"key":"B35","doi-asserted-by":"publisher","first-page":"4578","DOI":"10.1016\/j.vaccine.2013.07.058","article-title":"The virulence-related rhoptry protein 5 (ROP5) of Toxoplasma Gondii is a novel vaccine candidate against toxoplasmosis in mice.","volume":"31","author":"Zheng","year":"2013","journal-title":"Vaccine"},{"key":"B36","doi-asserted-by":"publisher","DOI":"10.14411\/fp.2016.028","article-title":"Analysis of structures and epitopes of a novel secreted protein MYR1 in Toxoplasma gondii.","volume":"63","author":"Zhou","year":"2016","journal-title":"Folia Parasitol."},{"key":"B37","doi-asserted-by":"publisher","DOI":"10.3389\/fmicb.2017.01733","article-title":"SAG4 DNA and peptide vaccination provides partial protection against T. gondii infection in BALB\/c mice.","volume":"8","author":"Zhou","year":"2017","journal-title":"Front. Microbiol."},{"key":"B38","doi-asserted-by":"publisher","first-page":"660","DOI":"10.1016\/j.immuni.2012.09.007","article-title":"The transcription factor T-bet is induced by multiple pathways and prevents an endogenous Th2 cell program during Th1 cell responses.","volume":"37","author":"Zhu","year":"2012","journal-title":"Immunity"}],"container-title":["Frontiers in Microbiology"],"original-title":[],"link":[{"URL":"https:\/\/www.frontiersin.org\/article\/10.3389\/fmicb.2019.00734\/full","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2019,4,9]],"date-time":"2019-04-09T06:15:06Z","timestamp":1554790506000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.frontiersin.org\/article\/10.3389\/fmicb.2019.00734\/full"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2019,4,9]]},"references-count":38,"alternative-id":["10.3389\/fmicb.2019.00734"],"URL":"https:\/\/doi.org\/10.3389\/fmicb.2019.00734","relation":{},"ISSN":["1664-302X"],"issn-type":[{"value":"1664-302X","type":"electronic"}],"subject":[],"published":{"date-parts":[[2019,4,9]]},"article-number":"734"}}