{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,29]],"date-time":"2026-09-29T04:18:11Z","timestamp":1790655491929,"version":"4.1.0"},"reference-count":73,"publisher":"Frontiers Media SA","license":[{"start":{"date-parts":[[2019,5,7]],"date-time":"2019-05-07T00:00:00Z","timestamp":1557187200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001807","name":"Funda\u00e7\u00e3o de Amparo \u00e0 Pesquisa do Estado de S\u00e3o Paulo","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100001807","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100003593","name":"Conselho Nacional de Desenvolvimento Cient\u00edfico e Tecnol\u00f3gico","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100003593","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001602","name":"Science Foundation Ireland","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100001602","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["frontiersin.org"],"crossmark-restriction":true},"short-container-title":["Front. Microbiol."],"abstract":"<jats:p>\n                    <jats:italic>Aspergillus<\/jats:italic>\n                    <jats:italic>fumigatus<\/jats:italic>\n                    , a saprophytic filamentous fungus, is a serious opportunistic pathogen of mammals and it is the primary causal agent of invasive aspergillosis (IA). Mitogen activated protein Kinases (MAPKs) are important components involved in diverse cellular processes in eukaryotes.\n                    <jats:italic>A. fumigatus<\/jats:italic>\n                    MpkC and SakA, the homologs of the\n                    <jats:italic>Saccharomyces cerevisiae<\/jats:italic>\n                    Hog1 are important to adaptations to oxidative and osmotic stresses, heat shock, cell wall damage, macrophage recognition, and full virulence. We performed protein pull-down experiments aiming to identify interaction partners of SakA and MpkC by mass spectrometry analysis. In presence of osmotic stress with sorbitol, 118, and 213 proteins were detected as possible protein interactors of SakA and MpkC, respectively. Under cell wall stress caused by congo red, 420 and 299 proteins were detected interacting with SakA and MpkC, respectively. Interestingly, a group of 78 and 256 proteins were common to both interactome analysis. Co-immunoprecipitation (Co-IP) experiments showed that SakA::GFP is physically associated with MpkC:3xHA upon osmotic and cell wall stresses. We also validated the association between SakA:GFP and the cell wall integrity MAPK MpkA:3xHA and the phosphatase PtcB:3xHA, under cell wall stress. We further characterized\n                    <jats:italic>A. fumigatus<\/jats:italic>\n                    PakA, the homolog of the\n                    <jats:italic>S. cerevisiae<\/jats:italic>\n                    sexual developmental serine\/threonine kinase Ste20, as a component of the SakA\/MpkC MAPK pathway. The\n                    <jats:italic>\u0394pakA<\/jats:italic>\n                    strain is more sensitive to cell wall damaging agents as congo red, calcofluor white, and caspofungin. Together, our data supporting the hypothesis that SakA and MpkC are part of an osmotic and general signal pathways involved in regulation of the response to the cell wall damage, oxidative stress, drug resistance, and establishment of infection. This manuscript describes an important biological resource to understand SakA and MpkC protein interactions. Further investigation of the biological roles played by these protein interactors will provide more opportunities to understand and combat IA.\n                  <\/jats:p>","DOI":"10.3389\/fmicb.2019.00918","type":"journal-article","created":{"date-parts":[[2019,5,7]],"date-time":"2019-05-07T00:44:16Z","timestamp":1557189856000},"update-policy":"https:\/\/doi.org\/10.3389\/crossmark-policy","source":"Crossref","is-referenced-by-count":35,"title":["Aspergillus fumigatus High Osmolarity Glycerol Mitogen Activated Protein Kinases SakA and MpkC Physically Interact During Osmotic and Cell Wall Stresses"],"prefix":"10.3389","volume":"10","author":[{"given":"Adriana Oliveira","family":"Manfiolli","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Eliciane Cevolani","family":"Mattos","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Leandro Jos\u00e9","family":"de Assis","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lilian Pereira","family":"Silva","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mevl\u00fct","family":"Ula\u015f","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Neil Andrew","family":"Brown","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rafael","family":"Silva-Rocha","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"\u00d6zg\u00fcr","family":"Bayram","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gustavo H.","family":"Goldman","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1965","published-online":{"date-parts":[[2019,5,7]]},"reference":[{"key":"B1","doi-asserted-by":"publisher","first-page":"S101","DOI":"10.1080\/13693780600900080","article-title":"Fungal responses to reactiveoxygen species.","volume":"44","author":"Aguirre","year":"2006","journal-title":"Med. Mycol."},{"key":"B2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0136932","article-title":"Network modeling reveals cross talk of MAP kinases during adaptation to caspofungin stress in Aspergillus fumigatus.","volume":"10","author":"Altwasser","year":"2015","journal-title":"PLoS One"},{"key":"B3","doi-asserted-by":"crossref","first-page":"2285","DOI":"10.1091\/mbc.e04-11-0987","article-title":"Specialization of the HOG pathway and its impact on differentiation and virulence of Cryptococcus neoformans.","volume":"16","author":"Bahn","year":"2005","journal-title":"Mol. Biol. Cell."},{"key":"B4","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pgen.1002816","article-title":"The Aspergillus nidulans MAPK module AnSte11-Ste50-Ste7-Fus3 controls development and secondary metabolism.","volume":"8","author":"Bayram","year":"2012","journal-title":"PLoS Genet."},{"key":"B5","doi-asserted-by":"publisher","first-page":"728","DOI":"10.1128\/EC.00008-15","article-title":"The Aspergillus fumigatus sitA phosphatase homologue is important for adhesion, cell wall integrity, biofilm formation, and virulence.","volume":"14","author":"Bom","year":"2015","journal-title":"Eukaryot. Cell"},{"key":"B6","doi-asserted-by":"crossref","first-page":"292","DOI":"10.1016\/j.sbi.2004.05.003","article-title":"Protein interaction networks from yeast to human.","volume":"14","author":"Bork","year":"2004","journal-title":"Curr. Opin. Struct. Biol."},{"key":"B7","doi-asserted-by":"crossref","first-page":"875","DOI":"10.2174\/138945005774912717","article-title":"Systemic fungal infections caused by Aspergillus species: epidemiology, infection process and virulence determinants.","volume":"6","author":"Brakhage","year":"2005","journal-title":"J. Curr. Drug Targets"},{"key":"B8","doi-asserted-by":"publisher","DOI":"10.1126\/scitranslmed.3004404","article-title":"Hidden killers: human fungal infections.","volume":"4","author":"Brown","year":"","journal-title":"Sci. Transl. Med."},{"key":"B9","doi-asserted-by":"publisher","DOI":"10.1126\/science.1222236","article-title":"Tackling human fungal infections.","volume":"336","author":"Brown","year":"","journal-title":"Science"},{"key":"B10","doi-asserted-by":"publisher","first-page":"243","DOI":"10.1007\/s12275-016-5510-4","article-title":"The contribution of Aspergillus fumigatus stress responses to virulence and fungicide resistance.","volume":"54","author":"Brown","year":"2016","journal-title":"J. Microbiol."},{"key":"B11","doi-asserted-by":"publisher","first-page":"11","DOI":"10.2165\/11585270-000000000-00000","article-title":"Echinocandin antifungal drugs in fungal infections: a comparison.","volume":"71","author":"Chen","year":"2011","journal-title":"Drugs"},{"key":"B12","doi-asserted-by":"publisher","first-page":"10352","DOI":"10.1073\/pnas.0601456103","article-title":"A highthroughput gene knockout procedure for Neurospora reveals functions for multiple transcription factors.","volume":"103","author":"Colot","year":"2006","journal-title":"Proc. Nat. Acad. Sci. U.S.A."},{"key":"B13","doi-asserted-by":"crossref","first-page":"1817","DOI":"10.1101\/gad.9.15.1817","article-title":"Ste20-like protein kinases are required for normal localization of cell growth and for cytokinesis in budding yeast.","volume":"9","author":"Cvrckov\u00e1","year":"1995","journal-title":"Genes Dev."},{"key":"B14","doi-asserted-by":"crossref","first-page":"207","DOI":"10.1128\/EC.5.1.207-211.2006","article-title":"The akuB(KU80) mutant deficient for nonhomologous end joining is a powerful tool for analyzing pathogenicity in Aspergillus fumigatus.","volume":"5","author":"da Silva Ferreira","year":"2006","journal-title":"Eukaryot. Cell"},{"key":"B15","doi-asserted-by":"publisher","first-page":"447","DOI":"10.1128\/CMR.00055-08","article-title":"Pathogenesis of Aspergillus fumigatus in invasive aspergillosis.","volume":"22","author":"Dagenais","year":"2009","journal-title":"Clin. Microbiol. Rev."},{"key":"B16","doi-asserted-by":"publisher","first-page":"e00840","DOI":"10.1128\/mBio.00840-18","article-title":"Regulation of Aspergillus nidulans CreA-mediated catabolite repression by the F-Box proteins Fbx23 and Fbx47.","author":"de Assis","year":"2018","journal-title":"mBio"},{"key":"B17","doi-asserted-by":"publisher","first-page":"655","DOI":"10.1111\/mmi.12785","article-title":"ChIP-seq reveals a role for CrzA in the Aspergillus fumigatus high-osmolarity glycerol response (HOG) signalling pathway.","volume":"94","author":"de Castro","year":"2014","journal-title":"Mol. Microbiol."},{"key":"B18","doi-asserted-by":"publisher","first-page":"3275","DOI":"10.1111\/febs.13323","article-title":"Osmostress-induced gene expression\u2014a model to understand how stress-activated protein kinases (SAPKs) regulate transcription.","volume":"282","author":"de Nadal","year":"2015","journal-title":"FEBS J."},{"key":"B19","doi-asserted-by":"publisher","first-page":"841","DOI":"10.1111\/mmi.13354","article-title":"Mitogen activated protein kinases SakA(HOG1) and MpkC collaborate for Aspergillus fumigatus virulence.","volume":"100","author":"de Oliveira Bruder Nascimento","year":"2016","journal-title":"Mol. Microbiol."},{"key":"B20","doi-asserted-by":"crossref","first-page":"4401","DOI":"10.1128\/iai.64.10.4401-4405.1996","article-title":"Attenuated virulence of uridine-uracil auxotrophs of Aspergillus fumigatus.","volume":"64","author":"D\u2019Enfert","year":"1996","journal-title":"Infect. Immun."},{"key":"B21","doi-asserted-by":"publisher","first-page":"163","DOI":"10.1007\/s00294-007-0174-6","article-title":"Functional analysis of a fungal endophyte stress-activated MAP kinase.","volume":"53","author":"Eaton","year":"2008","journal-title":"Curr. Genet."},{"key":"B22","doi-asserted-by":"crossref","first-page":"649","DOI":"10.1016\/0092-8674(90)90668-5","article-title":"FUS3 encodes a cdc2+\/CDC28-related kinase required for the transition from mitosis into conjugation.","volume":"60","author":"Elion","year":"1990","journal-title":"Cell"},{"key":"B23","doi-asserted-by":"publisher","first-page":"475","DOI":"10.4161\/viru.1.6.12985","article-title":"Methods for using Galleria mellonella as a model host to study fungal pathogenesis.","volume":"1","author":"Fuchs","year":"2010","journal-title":"Virulence"},{"key":"B24","doi-asserted-by":"publisher","DOI":"10.3389\/fmicb.2018.02518","article-title":"SakA and MpkC stress MAPKs show opposite and common functions during stress responses and development in Aspergillus nidulans.","volume":"9","author":"Garrido-Baz\u00e1n","year":"2018","journal-title":"Front. Microbiol."},{"key":"B25","doi-asserted-by":"crossref","first-page":"34","DOI":"10.1128\/EC.2.1.34-48.2003","article-title":"Expressed sequence tag analysis of the human pathogen Paracoccidioides brasiliensis yeast phase: identification of putative homologues of Candida albicans virulence and pathogenicity genes.","volume":"2","author":"Goldman","year":"2003","journal-title":"Eukaryot. Cell"},{"key":"B26","doi-asserted-by":"crossref","first-page":"685","DOI":"10.1067\/mai.2002.130179","article-title":"Allergic bronchopulmonary aspergillosis.","volume":"110","author":"Greenberger","year":"2002","journal-title":"J. Allergy Clin. Immunol."},{"key":"B27","doi-asserted-by":"publisher","first-page":"868","DOI":"10.1016\/j.fgb.2009.07.003","article-title":"Transcriptional profiling for Aspergillus nidulans HogA MAPK signaling pathway in response to fludioxonil and osmotic stress.","volume":"46","author":"Hagiwara","year":"2009","journal-title":"Fungal Genet. Biol."},{"key":"B28","doi-asserted-by":"publisher","first-page":"1327","DOI":"10.1105\/tpc.112.096156","article-title":"Mitogen-activated protein kinase signaling in plant-interacting fungi: distinct messages from conserved messengers.","volume":"24","author":"Hamel","year":"2012","journal-title":"Plant Cell"},{"key":"B29","doi-asserted-by":"publisher","first-page":"408","DOI":"10.1016\/j.ijmm.2011.04.008","article-title":"Shaping the fungal adaptome-stress responses of Aspergillus fumigatus.","volume":"301","author":"Hartmann","year":"2011","journal-title":"Int. J. Med. Microbiol."},{"key":"B30","doi-asserted-by":"publisher","DOI":"10.1186\/1471-2164-9-519","article-title":"The prediction of protein-protein interaction networks in rice blast fungus.","volume":"9","author":"He","year":"2008","journal-title":"BMC Genomics"},{"key":"B31","doi-asserted-by":"publisher","first-page":"802","DOI":"10.1094\/MPMI-11-11-0299","article-title":"The mitogen-activated protein kinase BcSak1 of Botrytis cinerea is required for pathogenic development and has broad regulatory functions beyond stress response.","volume":"25","author":"Heller","year":"2012","journal-title":"Mol. Plant Microbe Interact."},{"key":"B32","doi-asserted-by":"crossref","first-page":"839","DOI":"10.1111\/j.1365-2958.1990.tb00654.x","article-title":"High-efficiency transformation system for the biocontrol agents, Trichoderma spp.","volume":"4","author":"Herrera-Estrella","year":"1990","journal-title":"Mol. Microbiol."},{"key":"B33","doi-asserted-by":"publisher","first-page":"495","DOI":"10.1128\/EC.00277-14","article-title":"The SrkA kinase is part of the SakA mitogen-activated protein kinase interactome and regulates stress responses and development in Aspergillusnidulans.","volume":"14","author":"Jaimes-Arroyo","year":"2015","journal-title":"Eukaryot. Cell"},{"key":"B34","doi-asserted-by":"publisher","DOI":"10.1371\/journal.ppat.1005899","article-title":"SCF ubiquitin ligase F-box protein Fbx15 controls nuclear co-repressor localization, stress response and virulence of the human pathogen Aspergillus fumigatus.","volume":"12","author":"Johnk","year":"2016","journal-title":"PLoS Pathog."},{"key":"B35","doi-asserted-by":"crossref","first-page":"33","DOI":"10.1016\/S0065-2660(08)60245-X","article-title":"Meiotic and mitotic recombination in Aspergilllus and its chromosomal aberrations.","volume":"19","author":"Kafer","year":"1977","journal-title":"Adv. Genet."},{"key":"B36","doi-asserted-by":"crossref","first-page":"1153","DOI":"10.1046\/j.1365-2958.2002.03087.x","article-title":"SakA MAP kinase is involved in stress signal transduction, sexual development and spore viability in Aspergillus nidulans.","volume":"45","author":"Kawasaki","year":"2002","journal-title":"Mol. Microbiol."},{"key":"B37","doi-asserted-by":"crossref","first-page":"3595","DOI":"10.2174\/13816128113199990323","article-title":"Up-date on diagnostic strategies of invasive aspergillosis.","volume":"19","author":"Lackner","year":"2013","journal-title":"Curr. Pharm. Des."},{"key":"B38","doi-asserted-by":"publisher","first-page":"2088","DOI":"10.1016\/j.jmb.2015.01.016","article-title":"A comprehensive membrane interactome mapping of sho1p reveals Fps1p as a novel key player in the regulation of the HOG pathway in S. cerevisiae.","volume":"427","author":"Lam","year":"2015","journal-title":"J. Mol. Biol."},{"key":"B39","doi-asserted-by":"publisher","first-page":"180","DOI":"10.1016\/j.fgb.2011.12.006","article-title":"The Neurospora crassa OS MAPK pathway-activated transcription factor ASL-1 contributes to circadian rhythms in pathway responsive clock-controlled genes.","volume":"49","author":"Lamb","year":"2012","journal-title":"Fungal Genet. Biol."},{"key":"B40","doi-asserted-by":"publisher","first-page":"436","DOI":"10.1111\/j.1365-2958.2011.07581.x","article-title":"Aspergillus nidulans transcription factor AtfA interacts with the MAPK SakA to regulate general stress responses, development and spore functions.","volume":"80","author":"Lara-Rojas","year":"2011","journal-title":"Mol. Microbiol."},{"key":"B41","doi-asserted-by":"publisher","DOI":"10.3389\/fgene.2018.00104","article-title":"The interactomic analysis reveals pathogenic protein networks in Phomopsis longicolla underlying seed decay of soybean.","volume":"9","author":"Li","year":"2018","journal-title":"Front. Genet."},{"key":"B42","doi-asserted-by":"publisher","first-page":"99","DOI":"10.1007\/978-1-61779-539-8_7","article-title":"Gene disruption in Aspergillus fumigatus using a PCR-based strategy and in vivo recombination in yeast.","volume":"845","author":"Malavazi","year":"2012","journal-title":"Methods Mol. Biol."},{"key":"B43","doi-asserted-by":"publisher","DOI":"10.1111\/cmi.12770","article-title":"Aspergillus fumigatus protein phosphatase PpzA is involved in iron assimilation, secondary metabolite production, and virulence.","volume":"19","author":"Manfiolli","year":"2017","journal-title":"Cell. Microbiol."},{"key":"B44","doi-asserted-by":"publisher","first-page":"619","DOI":"10.1089\/omi.2010.0046","article-title":"Toward a genomic view of the gene expression program regulated by osmostress in yeast.","volume":"14","author":"Mart\u00ednez-Monta\u00f1\u00e9s","year":"2010","journal-title":"OMICS"},{"key":"B45","doi-asserted-by":"crossref","first-page":"905","DOI":"10.1099\/mic.0.28616-0","article-title":"The MAP kinase signal transduction network in Candida albicans.","volume":"152","author":"Monge","year":"2006","journal-title":"Microbiology"},{"key":"B46","doi-asserted-by":"publisher","first-page":"65","DOI":"10.1016\/j.ijmm.2014.11.003","article-title":"Characterization of sakA gene from pathogenic dimorphic fungus Penicillium marneffei.","volume":"305","author":"Nimmanee","year":"2015","journal-title":"Int. J. Med. Microbiol."},{"key":"B47","doi-asserted-by":"publisher","first-page":"4840","DOI":"10.1073\/pnas.1302265110","article-title":"Arabidopsis AHP2, AHP3, and AHP5 histidinephosphotransfer proteins function as redundant negative regulators ofdrought stress response.","volume":"110","author":"Nishiyama","year":"2013","journal-title":"Proc. Natl. Acad. Sci. U.S.A."},{"key":"B48","first-page":"153","article-title":"Mitogen-activated protein (MAP) kinase pathways: regulation and physiological functions.","volume":"22","author":"Pearson","year":"2001","journal-title":"Endocr. Rev."},{"key":"B49","doi-asserted-by":"publisher","first-page":"729","DOI":"10.1101\/SQB.1995.060.01.079","article-title":"Establishment of cell polarity in yeast.","volume":"60","author":"Pringle","year":"1995","journal-title":"Cold Spring Harb. Symp. Quant. Biol."},{"key":"B50","doi-asserted-by":"crossref","first-page":"4623","DOI":"10.1093\/emboj\/19.17.4623","article-title":"Yeast Cdc42 GTPase and Ste20 PAK-like kinase regulate Sho1-dependent activation of the Hog1 MAPK pathway.","volume":"19","author":"Raitt","year":"2000","journal-title":"EMBO J."},{"key":"B51","doi-asserted-by":"crossref","first-page":"1934","DOI":"10.1128\/EC.00178-06","article-title":"Novel mitogen-activated protein kinase MpkC of Aspergillus fumigatus is required for utilization of polyalcohol sugars.","volume":"5","author":"Reyes","year":"2006","journal-title":"Eukaryot. Cell"},{"key":"B52","doi-asserted-by":"crossref","first-page":"16861","DOI":"10.1074\/jbc.274.24.16861","article-title":"The Cdc42p GTPase is involved in a G2\/M morphogenetic checkpoint regulating the apical-isotropic switch and nuclear division in yeast.","volume":"274","author":"Richman","year":"1999","journal-title":"J. Biol. Chem."},{"key":"B53","doi-asserted-by":"publisher","first-page":"287","DOI":"10.1016\/j.fgb.2009.01.002","article-title":"Comparative genomics of MAP kinase and calcium-calcineurin signalling components in plant and human pathogenic fungi.","volume":"46","author":"Rispail","year":"2009","journal-title":"Fungal Genet. Biol."},{"key":"B54","doi-asserted-by":"crossref","first-page":"181","DOI":"10.1016\/j.tim.2007.02.001","article-title":"MAP kinase pathways as regulators of fungal virulence.","volume":"15","author":"Rom\u00e1n","year":"2007","journal-title":"Trends Microbiol."},{"key":"B55","author":"Sambrook","year":"2001","journal-title":"Molecular Cloning: A Laboratory Manual"},{"key":"B56","doi-asserted-by":"crossref","first-page":"339","DOI":"10.1007\/BF00340712","article-title":"High efficiency transformation of intact yeast cells using single stranded nucleic acids as a carrier.","volume":"16","author":"Schiestl","year":"1989","journal-title":"Curr. Genet."},{"key":"B57","doi-asserted-by":"publisher","first-page":"702","DOI":"10.1038\/376702a0","article-title":"Role for the Rho-family GTPase Cdc42 in yeast mating-pheromone signal pathway.","volume":"376","author":"Simon","year":"1995","journal-title":"Nature"},{"key":"B58","doi-asserted-by":"publisher","first-page":"S107","DOI":"10.3109\/13693786.2010.523852","article-title":"Pathogenicity of Aspergillus fumigatus mutants assessed in Galleria mellonella matches that in mice.","author":"Slater","year":"2011","journal-title":"Med. Mycol."},{"key":"B59","doi-asserted-by":"publisher","first-page":"S669","DOI":"10.1093\/cid\/civ725","article-title":"Potential microbiological effects of higher dosing of echinocandins.","volume":"61","author":"Steinbach","year":"2015","journal-title":"Clin. Infect. Dis."},{"key":"B60","doi-asserted-by":"publisher","DOI":"10.1101\/cshperspect.a019786","article-title":"Aspergillus fumigatus and related species.","volume":"5","author":"Sugui","year":"2014","journal-title":"Cold Spring Harb. Perspect. Med."},{"key":"B61","doi-asserted-by":"publisher","DOI":"10.1126\/scisignal.2004780","article-title":"Yeast osmosensors Hkr1 and Msb2 activate the Hog1 MAPK cascade by different mechanisms.","volume":"7","author":"Tanaka","year":"2014","journal-title":"Sci. Signal"},{"key":"B62","doi-asserted-by":"crossref","first-page":"385","DOI":"10.1016\/j.mib.2005.06.017","article-title":"Aspergillus fumigatus: saprophyte or pathogen?","volume":"8","author":"Tekaia","year":"2005","journal-title":"Curr. Opin. Microbiol."},{"key":"B63","doi-asserted-by":"publisher","first-page":"267","DOI":"10.1146\/annurev-phyto-102313-050143","article-title":"Protein kinases in plant-pathogenic fungi: conserved regulators of infection.","volume":"52","author":"Turra","year":"2014","journal-title":"Annu. Rev. Phytopathol."},{"key":"B64","doi-asserted-by":"publisher","DOI":"10.15252\/msb.20145112","article-title":"Phosphoproteomic analyses reveal novel cross-modulation mechanisms between two signaling pathways in yeast.","volume":"10","author":"Vaga","year":"2014","journal-title":"Mol. Syst. Biol."},{"key":"B65","doi-asserted-by":"crossref","first-page":"618","DOI":"10.1016\/j.fgb.2007.09.006","article-title":"The mitogen-activated protein kinase MpkA of Aspergillus fumigatus regulates cell wall signaling and oxidative stress response.","volume":"45","author":"Valiante","year":"2008","journal-title":"Fungal Genet. Biol."},{"key":"B66","doi-asserted-by":"publisher","first-page":"909","DOI":"10.1016\/j.fgb.2009.08.005","article-title":"The MpkA MAP kinase module regulates cell wall integrity signaling and pyomelanin formation in Aspergillus fumigatus.","volume":"46","author":"Valiante","year":"2009","journal-title":"Fungal Genet. Biol."},{"key":"B67","doi-asserted-by":"publisher","DOI":"10.3389\/fmicb.2015.00325","article-title":"The Aspergillus fumigatus cell wall integrity signaling pathway: drug target, compensatory pathways, and virulence.","volume":"6","author":"Valiante","year":"","journal-title":"Front. Microbiol."},{"key":"B68","doi-asserted-by":"publisher","first-page":"5145","DOI":"10.1128\/AAC.00683-15","article-title":"Hitting the caspofungin salvage pathway of human-pathogenic fungi with the novel lasso peptide humidimycin (MDN-0010).","volume":"59","author":"Valiante","year":"","journal-title":"Antimicrob. Agents Chemother."},{"key":"B69","doi-asserted-by":"publisher","first-page":"1378","DOI":"10.1094\/MPMI-04-13-0125-R","article-title":"The ATF\/CREB transcription factor Atf1 is essential for full virulence, deoxynivalenol production, and stress tolerance in the cereal pathogen Fusarium graminearum.","volume":"26","author":"Van Nguyen","year":"2013","journal-title":"Mol. Plant Microbe Interact."},{"key":"B70","doi-asserted-by":"crossref","first-page":"1570","DOI":"10.1128\/EC.00085-07","article-title":"Response regulators SrrA and SskA are central components of a phosphorelay system involved in stress signal transduction and asexual sporulation in Aspergillus nidulans.","volume":"6","author":"Vargas-Perez","year":"2007","journal-title":"Eukaryot. Cell"},{"key":"B71","doi-asserted-by":"publisher","first-page":"42","DOI":"10.1111\/mmi.12919","article-title":"High osmolarity glycerol response PtcB phosphatase is important for Aspergillus fumigatus virulence.","volume":"96","author":"Winkelstr\u00f6ter","year":"","journal-title":"Mol. Microbiol."},{"key":"B72","doi-asserted-by":"publisher","first-page":"1525","DOI":"10.1534\/g3.115.016766","article-title":"Systematic global analysis of genes encoding protein phosphatases in Aspergillus fumigatus.","volume":"5","author":"Winkelstr\u00f6ter","year":"","journal-title":"G3"},{"key":"B73","doi-asserted-by":"publisher","first-page":"4714","DOI":"10.1021\/pr900415b","article-title":"FPPI: Fusarium graminearum protein-protein interaction database.","volume":"8","author":"Zhao","year":"2009","journal-title":"J. Proteome Res."}],"updated-by":[{"DOI":"10.3389\/fmicb.2026.1893398","type":"correction","label":"Correction","source":"publisher","updated":{"date-parts":[[2026,6,23]],"date-time":"2026-06-23T00:00:00Z","timestamp":1782172800000}}],"container-title":["Frontiers in Microbiology"],"original-title":[],"link":[{"URL":"https:\/\/www.frontiersin.org\/articles\/10.3389\/fmicb.2019.00918\/full","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,7,9]],"date-time":"2026-07-09T10:57:06Z","timestamp":1783594626000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.frontiersin.org\/articles\/10.3389\/fmicb.2019.00918\/full"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2019,5,7]]},"references-count":73,"alternative-id":["10.3389\/fmicb.2019.00918"],"URL":"https:\/\/doi.org\/10.3389\/fmicb.2019.00918","relation":{},"ISSN":["1664-302X"],"issn-type":[{"value":"1664-302X","type":"electronic"}],"subject":[],"published":{"date-parts":[[2019,5,7]]},"article-number":"918"}}