{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,27]],"date-time":"2026-05-27T19:30:08Z","timestamp":1779910208157,"version":"3.53.1"},"reference-count":58,"publisher":"Frontiers Media SA","license":[{"start":{"date-parts":[[2019,7,2]],"date-time":"2019-07-02T00:00:00Z","timestamp":1562025600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["frontiersin.org"],"crossmark-restriction":true},"short-container-title":["Front. Microbiol."],"DOI":"10.3389\/fmicb.2019.01504","type":"journal-article","created":{"date-parts":[[2019,7,2]],"date-time":"2019-07-02T04:45:21Z","timestamp":1562042721000},"update-policy":"https:\/\/doi.org\/10.3389\/crossmark-policy","source":"Crossref","is-referenced-by-count":13,"title":["Identification of a Novel Plasmid Lineage Associated With the Dissemination of Metallo-\u03b2-Lactamase Genes Among Pseudomonads"],"prefix":"10.3389","volume":"10","author":[{"given":"Vincenzo","family":"Di Pilato","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alberto","family":"Antonelli","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tommaso","family":"Giani","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lucia","family":"Henrici De Angelis","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gian Maria","family":"Rossolini","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Simona","family":"Pollini","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1965","published-online":{"date-parts":[[2019,7,2]]},"reference":[{"key":"B1","doi-asserted-by":"publisher","first-page":"273","DOI":"10.1016\/j.jgar.2018.08.002","article-title":"Clinical cases of VIM-producing Pseudomonas mendocina from two burned patients.","volume":"14","author":"Almuzara","year":"2018","journal-title":"J. Glob. Antimicrob. Resist."},{"key":"B2","doi-asserted-by":"publisher","first-page":"e2091","DOI":"10.1128\/AAC.02091-2017","article-title":"Molecular Characterization of IMP-1-Producing Enterobacter cloacae Complex Isolates in Tokyo.","volume":"23","author":"Aoki","year":"2018","journal-title":"Antimicrob. Agents Chemother."},{"key":"B3","doi-asserted-by":"publisher","DOI":"10.1186\/1471-2164-9-75","article-title":"The RAST server: rapid annotations using subsystems technology.","volume":"9","author":"Aziz","year":"2008","journal-title":"BMC Genomics"},{"key":"B4","doi-asserted-by":"publisher","first-page":"455","DOI":"10.1089\/cmb.2012.0021","article-title":"SPAdes: a new genome assembly algorithm and its applications to single-cell sequencing.","volume":"19","author":"Bankevich","year":"2012","journal-title":"J. Comput. Biol."},{"key":"B5","doi-asserted-by":"publisher","first-page":"14073","DOI":"10.1073\/pnas.96.24.14073","article-title":"Global GacA-steered control of cyanide and exoprotease production in Pseudomonas fluorescens involves specific ribosome binding sites.","volume":"96","author":"Blumer","year":"1999","journal-title":"Proc. Natl. Acad. Sci. U.S.A."},{"key":"B6","doi-asserted-by":"publisher","first-page":"1060","DOI":"10.1093\/jac\/dks526","article-title":"Complete sequence of broad-host-range plasmid pNOR-2000 harbouring the metallo-\u03b2-lactamase gene blaV IM-2 from Pseudomonas aeruginosa.","volume":"68","author":"Bonnin","year":"2013","journal-title":"J. Antimicrob. Chemother."},{"key":"B7","doi-asserted-by":"publisher","first-page":"e2532","DOI":"10.1128\/AAC.02532-16","article-title":"Characterization of the pJB12 plasmid from Pseudomonas aeruginosa reveals Tn6352, a novel putative transposon associated with mobilization of the blaV IM-2-harboring In58 integron.","volume":"61","author":"Botelho","year":"2017","journal-title":"Antimicrob. Agents Chemother."},{"key":"B8","doi-asserted-by":"publisher","first-page":"873","DOI":"10.1093\/jac\/dkx517","article-title":"Two decades of blaV IM-2-producing Pseudomonas aeruginosa dissemination: an interplay between mobile genetic elements and successful clones.","volume":"73","author":"Botelho","year":"2018","journal-title":"J. Antimicrob. Chemother."},{"key":"B9","doi-asserted-by":"publisher","first-page":"67","DOI":"10.1007\/s00253-005-0119-112","article-title":"Genetic organization of a plasmid from an industrial wastewater bioreactor.","volume":"71","author":"Bramucci","year":"2006","journal-title":"Appl. Microbiol. Biotechnol."},{"key":"B10","doi-asserted-by":"publisher","first-page":"2227","DOI":"10.1128\/AAC.01707-1708","article-title":"Resistance Plasmid Families in Enterobacteriaceae.","volume":"53","author":"Carattoli","year":"2009","journal-title":"Antimicrob. Agents Chemother."},{"key":"B11","doi-asserted-by":"publisher","first-page":"219","DOI":"10.1016\/j.mimet.2005.03.018","article-title":"Identification of plasmids by PCR-based replicon typing.","volume":"63","author":"Carattoli","year":"2005","journal-title":"J. Microbiol. Methods"},{"key":"B12","doi-asserted-by":"publisher","first-page":"467","DOI":"10.1046\/j.1365-2958.2000.01986.x","article-title":"Control of plasmid DNA replication by iterons: no longer paradoxical.","volume":"37","author":"Chattoraj","year":"2000","journal-title":"Mol. Microbiol."},{"key":"B13","doi-asserted-by":"publisher","first-page":"732","DOI":"10.1128\/jb.175.3.732-740.1993","article-title":"Nucleotide sequence analysis of a transposon (Tn5393) carrying streptomycin resistance genes in Erwinia amylovora and other gram-negative bacteria.","volume":"175","author":"Chiou","year":"1993","journal-title":"J. Bacteriol."},{"key":"B14","doi-asserted-by":"publisher","first-page":"67","DOI":"10.1093\/jac\/dkt311","article-title":"Characterization of plasmid pAX22, encoding VIM-1 metallo-\u03b2-lactamase, reveals a new putative mechanism of In70 integron mobilization.","volume":"69","author":"Di Pilato","year":"2014","journal-title":"J. Antimicrob. Chemother."},{"key":"B15","doi-asserted-by":"publisher","first-page":"1583","DOI":"10.1128\/AAC.04047-4014","article-title":"Tn6249, a new Tn6162 transposon derivative carrying a double-integron platform and involved with acquisition of the blaV IM-1 metallo-\u03b2-lactamase gene in Pseudomonas aeruginosa.","volume":"59","author":"Di Pilato","year":"2015","journal-title":"Antimicrob. Agents Chemother."},{"key":"B16","doi-asserted-by":"publisher","first-page":"1522","DOI":"10.1128\/aac.47.5.1522-1528.2003","article-title":"IMP-12, a new plasmid-encoded metallo-\u03b2-lactamase from a Pseudomonas putida clinical isolate.","volume":"47","author":"Docquier","year":"2003","journal-title":"Antimicrob. Agents Chemother."},{"key":"B17","doi-asserted-by":"publisher","first-page":"351","DOI":"10.2165\/00003495-200767030-200767033","article-title":"The epidemiology, pathogenesis and treatment of Pseudomonas aeruginosa Infections.","volume":"67","author":"Driscoll","year":"2007","journal-title":"Drugs"},{"key":"B18","doi-asserted-by":"publisher","first-page":"274","DOI":"10.1093\/jac\/dkn470","article-title":"Characterization of plasmids harbouring qnrS1, qnrB2 and qnrB19 genes in Salmonella.","volume":"63","author":"Garc\u00eda-Fern\u00e1ndez","year":"2009","journal-title":"J. Antimicrob. Chemother."},{"key":"B19","doi-asserted-by":"publisher","first-page":"1987","DOI":"10.1093\/jac\/dkr225","article-title":"Multilocus sequence typing of IncN plasmids.","volume":"66","author":"Garc\u00eda-Fern\u00e1ndez","year":"2011","journal-title":"J. Antimicrob. Chemother."},{"key":"B20","doi-asserted-by":"publisher","first-page":"657","DOI":"10.1111\/j.1574-6976.2009.00168.x","article-title":"The diversity of conjugative relaxases and its application in plasmid classification.","volume":"33","author":"Garcill\u00e1n-Barcia","year":"2009","journal-title":"FEMS Microbiol. Rev."},{"key":"B21","doi-asserted-by":"publisher","first-page":"664","DOI":"10.1093\/jac\/dkx453","article-title":"Italian nationwide survey on Pseudomonas aeruginosa from invasive infections: activity of ceftolozane\/tazobactam and comparators, and molecular epidemiology of carbapenemase producers.","volume":"73","author":"Giani","year":"2018","journal-title":"J. Antimicrob. Chemother."},{"key":"B22","doi-asserted-by":"publisher","first-page":"2216","DOI":"10.1128\/AAC.06005-11","article-title":"VIM-1-producing Pseudomonas mosselii isolates in Italy, predating known VIM-producing index strains.","volume":"56","author":"Giani","year":"2012","journal-title":"Antimicrob. Agents Chemother."},{"key":"B23","doi-asserted-by":"publisher","first-page":"W181","DOI":"10.1093\/nar\/gkn179","article-title":"The CGView Server: a comparative genomics tool for circular genomes.","volume":"36","author":"Grant","year":"2008","journal-title":"Nucleic Acids Res."},{"key":"B24","doi-asserted-by":"publisher","first-page":"856","DOI":"10.1046\/j.1462-2920.2002.00305.x","article-title":"Complete sequence of the IncP-9 TOL plasmid pWW0 from Pseudomonas putida.","volume":"4","author":"Greated","year":"2002","journal-title":"Environ. Microbiol."},{"key":"B25","doi-asserted-by":"publisher","first-page":"5715","DOI":"10.1093\/nar\/gku194","article-title":"Key components of the eight classes of type IV secretion systems involved in bacterial conjugation or protein secretion.","volume":"42","author":"Guglielmini","year":"2014","journal-title":"Nucleic Acids Res."},{"key":"B26","doi-asserted-by":"publisher","first-page":"4728","DOI":"10.1128\/JB.187.14.4728-4738.2005","article-title":"The IncP-6 Plasmid Rms149 consists of a small mobilizable backbone with multiple large insertions.","volume":"187","author":"Haines","year":"2005","journal-title":"J. Bacteriol."},{"key":"B27","doi-asserted-by":"publisher","first-page":"iii2","DOI":"10.1093\/jac\/dky027","article-title":"Treatment of infections caused by multidrug-resistant Gram-negative bacteria: report of the British society for antimicrobial chemotherapy\/healthcare infection society\/british infection association joint working party.","volume":"73","author":"Hawkey","year":"2018","journal-title":"J. Antimicrob. Chemother."},{"key":"B28","doi-asserted-by":"publisher","first-page":"W236","DOI":"10.1093\/nar\/gkw370","article-title":"Evolview v2: an online visualization and management tool for customized and annotated phylogenetic trees.","volume":"44","author":"He","year":"2016","journal-title":"Nucleic Acids Res."},{"key":"B29","doi-asserted-by":"crossref","DOI":"10.1128\/9781555817381.ch42","article-title":"\u201cPseudomonas,\u201d in","author":"H\u00f8iby","year":"2015","journal-title":"Manual of Clinical Microbiology"},{"key":"B30","doi-asserted-by":"publisher","first-page":"81","DOI":"10.3947\/ic.2015.47.2.81","article-title":"Epidemiology and characteristics of metallo-\u03b2-lactamase-producing Pseudomonas aeruginosa.","volume":"47","author":"Hong","year":"2015","journal-title":"Infect. Chemother."},{"key":"B31","first-page":"655","article-title":"\u201cSimilarity analysis of DNAs,\u201d in","author":"Johnson","year":"1994","journal-title":"Methods for General and Molecular Bacteriology"},{"key":"B32","doi-asserted-by":"publisher","first-page":"43","DOI":"10.1016\/j.plasmid.2012.03.001","article-title":"Expansion of the IncX plasmid family for improved identification and typing of novel plasmids in drug-resistant Enterobacteriaceae.","volume":"68","author":"Johnson","year":"2012","journal-title":"Plasmid"},{"key":"B33","doi-asserted-by":"publisher","first-page":"1584","DOI":"10.1128\/aac.43.7.1584","article-title":"Cloning and characterization of blaV IM, a new integron-borne metallo-\u03b2-lactamase gene from a Pseudomonas aeruginosa clinical isolate.","volume":"43","author":"Lauretti","year":"1999","journal-title":"Antimicrob. Agents Chemother."},{"key":"B34","doi-asserted-by":"publisher","first-page":"3099","DOI":"10.1128\/AAC.01093-1097","article-title":"Complete sequence of p07-406, a 24,179-base-pair plasmid harboring the blaV IM-7 metallo-\u03b2-lactamase gene in a Pseudomonas aeruginosa isolate from the United States.","volume":"52","author":"Li","year":"2008","journal-title":"Antimicrob. Agents Chemother."},{"key":"B35","doi-asserted-by":"publisher","DOI":"10.3389\/fmicb.2019.00404","article-title":"Identification of diverse integron and plasmid structures carrying a novel carbapenemase among Pseudomonas Species.","volume":"10","author":"Liapis","year":"2019","journal-title":"Front. Microbiol."},{"key":"B36","doi-asserted-by":"publisher","first-page":"143","DOI":"10.1007\/978-3-319-75241-9_6","article-title":"Coupling proteins in type IV secretion.","volume":"413","author":"Llosa","year":"2017","journal-title":"Curr. Top. Microbiol. Immuno."},{"key":"B37","doi-asserted-by":"publisher","first-page":"4051","DOI":"10.1128\/JCM.40.11.4051-4055.2002","article-title":"Nosocomial infections caused by multidrug-resistant isolates of Pseudomonas putida producing VIM-1 metallo-\u03b2-Lactamase.","volume":"40","author":"Lombardi","year":"2002","journal-title":"J. Clin. Microbiol."},{"key":"B38","doi-asserted-by":"publisher","first-page":"2169","DOI":"10.1128\/AAC.06048-6011","article-title":"Diverse mobilized class 1 integrons are common in the chromosomes of pathogenic Pseudomonas aeruginosa clinical isolates.","volume":"56","author":"Martinez","year":"2012","journal-title":"Antimicrob. Agents Chemother."},{"key":"B39","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0081604","article-title":"Antibiotic resistance determinants in a Pseudomonas putida strain isolated from a Hospital.","volume":"9","author":"Molina","year":"2014","journal-title":"PLoS One"},{"key":"B40","doi-asserted-by":"publisher","first-page":"1334","DOI":"10.1128\/AAC.04639-14","article-title":"Isolation of VIM-2-producing Pseudomonas monteilii clinical strains disseminated in a tertiary Hospital in northern Spain.","volume":"59","author":"Ocampo-Sosa","year":"2015","journal-title":"Antimicrob. Agents Chemother."},{"key":"B41","doi-asserted-by":"publisher","first-page":"423","DOI":"10.1016\/j.dib.2017.04.024","article-title":"A curated dataset of complete Enterobacteriaceae plasmids compiled from the NCBI nucleotide database.","volume":"12","author":"Orlek","year":"2017","journal-title":"Data Brief"},{"key":"B42","doi-asserted-by":"publisher","first-page":"3691","DOI":"10.1093\/bioinformatics\/btv421","article-title":"Roary: rapid large-scale prokaryote pan genome analysis.","volume":"31","author":"Page","year":"2015","journal-title":"Bioinformatics"},{"key":"B43","doi-asserted-by":"publisher","first-page":"e88","DOI":"10.1128\/CMR.00088-17","article-title":"Mobile genetic elements associated with antimicrobial resistance.","volume":"31","author":"Partridge","year":"2018","journal-title":"Clin. Microbiol. Rev."},{"key":"B44","doi-asserted-by":"publisher","first-page":"106","DOI":"10.1016\/j.meegid.2017.10.026","article-title":"The current status on the taxonomy of Pseudomonas revisited: an update.","volume":"57","author":"Peix","year":"2018","journal-title":"Infect. Genet. Evol."},{"key":"B45","doi-asserted-by":"publisher","first-page":"5929","DOI":"10.1128\/AAC.02372-2314","article-title":"Extensively drug-resistant Pseudomonas aeruginosa isolates containing blaV IM-2 and elements of Salmonella genomic island 2: a new genetic resistance determinant in Northeast Ohio.","volume":"58","author":"Perez","year":"2014","journal-title":"Antimicrob. Agents Chemother."},{"key":"B46","doi-asserted-by":"publisher","DOI":"10.1098\/rsob.150175","article-title":"Genomic islands 1 and 2 play key roles in the evolution of extensively drug-resistant ST235 isolates of Pseudomonas aeruginosa.","volume":"6","author":"Roy Chowdhury","year":"2016","journal-title":"Open Biol."},{"key":"B47","doi-asserted-by":"publisher","first-page":"e1954","DOI":"10.1128\/AAC.01954-1916","article-title":"Plasmids of Distinct IncK Lineages Show Compatible Phenotypes.","volume":"61","author":"Rozwandowicz","year":"2017","journal-title":"Antimicrob. Agents Chemother."},{"key":"B48","doi-asserted-by":"publisher","first-page":"3000","DOI":"10.1093\/jac\/dkv222","article-title":"Sequencing of plasmids pAMBL1 and pAMBL2 from Pseudomonas aeruginosa reveals a blaV IM-1 amplification causing high-level carbapenem resistance.","volume":"70","author":"San Millan","year":"2015","journal-title":"J. Antimicrob. Chemother."},{"key":"B49","doi-asserted-by":"publisher","first-page":"2929","DOI":"10.1099\/mic.0.2008\/017939-17930","article-title":"Diversity of IncP-9 plasmids of Pseudomonas.","volume":"154","author":"Sevastsyanovich","year":"2008","journal-title":"Microbiology"},{"key":"B50","doi-asserted-by":"publisher","DOI":"10.3389\/fmicb.2015.00242","article-title":"Genomics of microbial plasmids: classification and identification based on replication and transfer systems and host taxonomy.","volume":"6","author":"Shintani","year":"2015","journal-title":"Front. Microbiol."},{"key":"B51","doi-asserted-by":"publisher","first-page":"434","DOI":"10.1128\/MMBR.00020-10","article-title":"Mobility of Plasmids.","volume":"74","author":"Smillie","year":"2010","journal-title":"Microbiol. Mol. Biol. Rev."},{"key":"B52","doi-asserted-by":"publisher","first-page":"1009","DOI":"10.1093\/bioinformatics\/btr039","article-title":"Easyfig: a genome comparison visualizer.","volume":"27","author":"Sullivan","year":"2011","journal-title":"Bioinformatics"},{"key":"B53","doi-asserted-by":"publisher","first-page":"87","DOI":"10.1093\/jac\/dki150","article-title":"The genetic background for streptomycin resistance in Escherichia coli influences the distribution of MICs.","volume":"56","author":"Sunde","year":"2005","journal-title":"J. Antimicrob. Chemother."},{"key":"B54","doi-asserted-by":"publisher","first-page":"6045","DOI":"10.1128\/JB.00277-210","article-title":"Predicting plasmid promiscuity based on genomic signature.","volume":"192","author":"Suzuki","year":"2010","journal-title":"J. Bacteriol."},{"key":"B55","doi-asserted-by":"publisher","first-page":"7017","DOI":"10.1128\/AAC.02934-14","article-title":"A blaV IM-2 plasmid disseminating in extensively drug-Resistant clinical Pseudomonas aeruginosa and Serratia marcescens isolates.","volume":"58","author":"Vilacoba","year":"2014","journal-title":"Antimicrob. Agents Chemother."},{"key":"B56","doi-asserted-by":"publisher","first-page":"451","DOI":"10.1016\/j.ijantimicag.2014.02.006","article-title":"Multiclonal spread of VIM-1-producing Enterobacter cloacae isolates associated with In624 and In488 integrons located in an IncHI2 plasmid.","volume":"43","author":"Villa","year":"2014","journal-title":"Int. J. Antimicrob. Agents"},{"key":"B57","doi-asserted-by":"publisher","first-page":"2518","DOI":"10.1093\/jac\/dkq347","article-title":"Replicon sequence typing of IncF plasmids carrying virulence and resistance determinants.","volume":"65","author":"Villa","year":"2010","journal-title":"J. Antimicrob. Chemother."},{"key":"B58","doi-asserted-by":"publisher","first-page":"2224","DOI":"10.1128\/AAC.45.8.2224-2228.2001","article-title":"Metallo-\u03b2-lactamases in clinical Pseudomonas isolates in Taiwan and identification of VIM-3, a novel variant of the VIM-2 enzyme.","volume":"45","author":"Yan","year":"2001","journal-title":"Antimicrob. Agents Chemother."}],"container-title":["Frontiers in Microbiology"],"original-title":[],"link":[{"URL":"https:\/\/www.frontiersin.org\/article\/10.3389\/fmicb.2019.01504\/full","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2021,1,9]],"date-time":"2021-01-09T02:25:09Z","timestamp":1610159109000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.frontiersin.org\/article\/10.3389\/fmicb.2019.01504\/full"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2019,7,2]]},"references-count":58,"alternative-id":["10.3389\/fmicb.2019.01504"],"URL":"https:\/\/doi.org\/10.3389\/fmicb.2019.01504","relation":{},"ISSN":["1664-302X"],"issn-type":[{"value":"1664-302X","type":"electronic"}],"subject":[],"published":{"date-parts":[[2019,7,2]]},"article-number":"1504"}}