{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,29]],"date-time":"2026-09-29T19:39:21Z","timestamp":1790710761427,"version":"4.1.0"},"reference-count":48,"publisher":"Frontiers Media SA","license":[{"start":{"date-parts":[[2019,7,31]],"date-time":"2019-07-31T00:00:00Z","timestamp":1564531200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001659","name":"Deutsche Forschungsgemeinschaft","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100001659","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["frontiersin.org"],"crossmark-restriction":true},"short-container-title":["Front. Microbiol."],"DOI":"10.3389\/fmicb.2019.01740","type":"journal-article","created":{"date-parts":[[2019,7,31]],"date-time":"2019-07-31T14:07:39Z","timestamp":1564582059000},"update-policy":"https:\/\/doi.org\/10.3389\/crossmark-policy","source":"Crossref","is-referenced-by-count":22,"title":["Biomimetic Human Tissue Model for Long-Term Study of Neisseria gonorrhoeae Infection"],"prefix":"10.3389","volume":"10","author":[{"given":"Motaharehsadat","family":"Heydarian","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tao","family":"Yang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Matthias","family":"Schweinlin","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Maria","family":"Steinke","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Heike","family":"Walles","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Thomas","family":"Rudel","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Vera","family":"Kozjak-Pavlovic","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1965","published-online":{"date-parts":[[2019,7,31]]},"reference":[{"key":"B1","doi-asserted-by":"publisher","first-page":"507","DOI":"10.1084\/jem.168.2.507","article-title":"Purification and characterization of eight class 5 outer membrane protein variants from a clone of Neisseria meningitidis serogroup A","volume":"168","author":"Achtman","year":"1988","journal-title":"J. Exp. Med."},{"key":"B2","doi-asserted-by":"publisher","first-page":"636","DOI":"10.1093\/infdis\/173.3.636","article-title":"The pathogenesis of gonococcal urethritis in men: confocal and immunoelectron microscopic analysis of urethral exudates from men infected with Neisseria gonorrhoeae","volume":"173","author":"Apicella","year":"1996","journal-title":"J. Infect. Dis."},{"key":"B3","doi-asserted-by":"publisher","first-page":"836","DOI":"10.1093\/humrep\/16.5.836","article-title":"Endometrial stromal cells regulate epithelial cell growth in vitro: a new co-culture model","volume":"16","author":"Arnold","year":"2001","journal-title":"Hum. Reprod."},{"key":"B4","doi-asserted-by":"publisher","first-page":"864","DOI":"10.1093\/humrep\/deh722","article-title":"A novel organotypic culture model for normal human endometrium: regulation of epithelial cell proliferation by estradiol and medroxyprogesterone acetate","volume":"20","author":"Bl\u00e4uer","year":"2005","journal-title":"Hum. Reprod."},{"key":"B5","doi-asserted-by":"publisher","first-page":"1775","DOI":"10.1242\/dev.148478","article-title":"Development of organoids from mouse and human endometrium showing endometrial epithelium physiology and long-term expandability","volume":"144","author":"Boretto","year":"2017","journal-title":"Development"},{"key":"B6","doi-asserted-by":"publisher","first-page":"8","DOI":"10.1016\/j.jri.2011.08.002","article-title":"Innate immune mediator profiles and their regulation in a novel polarized immortalized epithelial cell model derived from human endocervix","volume":"92","author":"Buckner","year":"2011","journal-title":"J. Reprod. Immunol."},{"key":"B7","doi-asserted-by":"publisher","first-page":"32","DOI":"10.1046\/j.1365-2958.2000.01694.x","article-title":"Interaction of primary human endometrial cells with Neisseria gonorrhoeae expressing green fluorescent protein","volume":"35","author":"Christodoulides","year":"2000","journal-title":"Mol. Microbiol."},{"key":"B8","doi-asserted-by":"publisher","first-page":"a025676","DOI":"10.1101\/cshperspect.a025676","article-title":"Biologic scaffolds","volume":"7","author":"Costa","year":"2017","journal-title":"Cold Spring. Harb. Perspect. Med."},{"key":"B9","doi-asserted-by":"publisher","first-page":"363","DOI":"10.1038\/nrmicro885","article-title":"Type IV pilus structure and bacterial pathogenicity","volume":"2","author":"Craig","year":"2004","journal-title":"Nat. Rev. Microbiol."},{"key":"B10","doi-asserted-by":"publisher","first-page":"127","DOI":"10.1016\/S0165-0378(99)00046-7","article-title":"MUC1\/episialin: a critical barrier in the female reproductive tract","volume":"45","author":"DeSouza","year":"1999","journal-title":"J. Reprod. Immunol."},{"key":"B11","doi-asserted-by":"publisher","first-page":"965","DOI":"10.1128\/CMR.17.4.965-981.2004","article-title":"The molecular mechanisms used by Neisseria gonorrhoeae to initiate infection differ between men and women","volume":"17","author":"Edwards","year":"2004","journal-title":"Clin. Microbiol. Rev."},{"key":"B12","doi-asserted-by":"publisher","first-page":"1042","DOI":"10.1111\/cmi.12099","article-title":"Neisseria gonorrhoeae breaches the apical junction of polarized epithelial cells for transmigration by activating EGFR","volume":"15","author":"Edwards","year":"2013","journal-title":"Cell. Microbiol."},{"key":"B13","doi-asserted-by":"publisher","first-page":"155","DOI":"10.1016\/S0049-0172(81)80001-7","article-title":"Disseminated gonococcal infection (DGI) and gonococcal arthritis (GCA): I. bacteriology, epidemiology, host factors, pathogen factors, and pathology","volume":"10","author":"Eisenstein","year":"1981","journal-title":"Semin. Arthritis. Rheum."},{"key":"B14","doi-asserted-by":"publisher","first-page":"5840","DOI":"10.1128\/IAI.69.9.5840-5848.2001","article-title":"Distinct proinflammatory host responses to Neisseria gonorrhoeae infection in immortalized human cervical and vaginal epithelial cells","volume":"69","author":"Fichorova","year":"2001","journal-title":"Infect. Immun."},{"key":"B15","doi-asserted-by":"publisher","first-page":"7211","DOI":"10.1128\/JB.00724-06","article-title":"AmiC functions as an N-acetylmuramyl-l-alanine amidase necessary for cell separation and can promote autolysis in Neisseria gonorrhoeae","volume":"188","author":"Garcia","year":"2006","journal-title":"J. Bacteriol."},{"key":"B16","doi-asserted-by":"publisher","first-page":"5808","DOI":"10.1128\/IAI.70.10.5808-5815.2002","article-title":"Immortalization of human urethral epithelial cells: a model for the study of the pathogenesis of and the inflammatory cytokine response to Neisseria gonorrhoeae infection","volume":"70","author":"Harvey","year":"2002","journal-title":"Infect. Immun."},{"key":"B17","doi-asserted-by":"publisher","first-page":"8989","DOI":"10.1038\/ncomms9989","article-title":"The Notch and Wnt pathways regulate stemness and differentiation in human fallopian tube organoids","volume":"6","author":"Kessler","year":"2015","journal-title":"Nat. Commun."},{"key":"B18","doi-asserted-by":"publisher","first-page":"4266","DOI":"10.1128\/IAI.00215-06","article-title":"Low-phosphate-dependent invasion resembles a general way for Neisseria gonorrhoeae to enter host cells","volume":"74","author":"K\u00fchlewein","year":"2006","journal-title":"Infect. Immun."},{"key":"B19","doi-asserted-by":"publisher","first-page":"e01049","DOI":"10.1128\/IAI.01049-16","article-title":"Human three-dimensional endometrial epithelial cell model to study host interactions with vaginal bacteria and Neisseria gonorrhoeae","volume":"85","author":"\u0141aniewski","year":"2017","journal-title":"Infect. Immun."},{"key":"B20","doi-asserted-by":"publisher","first-page":"63","DOI":"10.1186\/s13287-017-0500-y","article-title":"Urethral reconstruction with autologous urine-derived stem cells seeded in three-dimensional porous small intestinal submucosa in a rabbit model","volume":"8","author":"Liu","year":"2017","journal-title":"Stem Cell Res. Ther."},{"key":"B21","doi-asserted-by":"publisher","first-page":"1307","DOI":"10.1002\/j.1460-2075.1991.tb07649.x","article-title":"Phase variation of the opacity outer membrane protein controls invasion by Neisseria gonorrhoeae into human epithelial cells","volume":"10","author":"Makino","year":"1991","journal-title":"EMBO J."},{"key":"B22","doi-asserted-by":"publisher","first-page":"382","DOI":"10.1016\/S0171-2985(11)80352-9","article-title":"Lipo-oligosaccharides (LOS) of mucosal pathogens: molecular mimicry and host-modification of LOS","volume":"187","author":"Mandrell","year":"1993","journal-title":"Immunobiology"},{"key":"B23","doi-asserted-by":"publisher","first-page":"1973","DOI":"10.1016\/j.humpath.2013.04.009","article-title":"Epithelial-to-mesenchymal transition and stem cells in endometrial cancer","volume":"44","author":"Mirantes","year":"2013","journal-title":"Hum. Pathol."},{"key":"B24","doi-asserted-by":"publisher","first-page":"105","DOI":"10.1111\/j.1574-695X.1996.tb00127.x","article-title":"Molecular mimicry of host structures by bacterial lipopolysaccharides and its contribution to disease","volume":"16","author":"Moran","year":"1996","journal-title":"FEMS Immunol. Med. Microbiol."},{"key":"B25","doi-asserted-by":"publisher","first-page":"e68761","DOI":"10.1371\/journal.pone.0068761","article-title":"Gastrointestinal cell lines form polarized epithelia with an adherent mucus layer when cultured in semi-wet interfaces with mechanical stimulation","volume":"8","author":"Navabi","year":"2013","journal-title":"PLoS ONE"},{"key":"B26","doi-asserted-by":"publisher","first-page":"3538","DOI":"10.1128\/AAC.00325-11","article-title":"Is Neisseria gonorrhoeae initiating a future era of untreatable gonorrhea?: detailed characterization of the first strain with high-level resistance to ceftriaxone","volume":"55","author":"Ohnishi","year":"2011","journal-title":"Antimicrob. Agents Chemother."},{"key":"B27","doi-asserted-by":"publisher","first-page":"1173","DOI":"10.1128\/MCB.06388-11","article-title":"Sam50 functions in mitochondrial intermembrane space bridging and biogenesis of respiratory complexes","volume":"32","author":"Ott","year":"2012","journal-title":"Mol. Cell. Biol."},{"key":"B28","doi-asserted-by":"publisher","first-page":"433","DOI":"10.1128\/IAI.00711-09","article-title":"Mouse strain-dependent differences in susceptibility to Neisseria gonorrhoeae infection and induction of innate immune responses","volume":"78","author":"Packiam","year":"2010","journal-title":"Infect. Immun."},{"key":"B29","doi-asserted-by":"publisher","first-page":"1027","DOI":"10.1089\/ten.tec.2011.0082","article-title":"Raman spectroscopy: a noninvasive analysis tool for the discrimination of human skin cells","volume":"17","author":"Pudlas","year":"2011","journal-title":"Tissue Eng. Part C Methods."},{"key":"B30","doi-asserted-by":"crossref","first-page":"255","DOI":"10.1128\/IAI.8.2.255-263.1973","article-title":"Role of pili in the virulence of Neisseria gonorrhoeae","volume":"8","author":"Punsalang","year":"1973","journal-title":"Infect. Immun."},{"key":"B31","doi-asserted-by":"publisher","first-page":"132","DOI":"10.1095\/biolreprod.112.103366","article-title":"Microbial products alter the expression of membrane-associated mucin and antimicrobial peptides in a three-dimensional human endocervical epithelial cell model","volume":"87","author":"Radtke","year":"2012","journal-title":"Biol. Reprod."},{"key":"B32","doi-asserted-by":"publisher","first-page":"915","DOI":"10.1016\/S0161-5890(99)00114-5","article-title":"The contrasting mechanisms of serum resistance of Neisseria gonorrhoeae and group B Neisseria meningitidis","volume":"36","author":"Ram","year":"1999","journal-title":"Mol. Immunol."},{"key":"B33","doi-asserted-by":"publisher","first-page":"393","DOI":"10.1016\/j.chom.2007.11.002","article-title":"Host glycoprotein Gp96 and scavenger receptor SREC interact with PorB of disseminating Neisseria gonorrhoeae in an epithelial invasion pathway","volume":"2","author":"Rechner","year":"2007","journal-title":"Cell. Host Microbe."},{"key":"B34","doi-asserted-by":"publisher","first-page":"665","DOI":"10.1146\/annurev-micro-090816-093530","article-title":"Neisseria gonorrhoeae: drug resistance, mouse models, and vaccine development","volume":"71","author":"Rice","year":"2017","journal-title":"Annu. Rev. Microbiol."},{"key":"B35","doi-asserted-by":"publisher","first-page":"290","DOI":"10.1016\/j.micinf.2011.11.002","article-title":"Neisseria gonorrhoeae induced disruption of cell junction complexes in epithelial cells of the human genital tract","volume":"14","author":"Rodr\u00edguez-Tirado","year":"2012","journal-title":"Microbes. Infect."},{"key":"B36","doi-asserted-by":"publisher","first-page":"676","DOI":"10.1038\/nmeth.2019","article-title":"Fiji: an open-source platform for biological-image analysis","volume":"9","author":"Schindelin","year":"2012","journal-title":"Nat. Methods"},{"key":"B37","doi-asserted-by":"publisher","first-page":"274","DOI":"10.1186\/1471-2105-11-274","article-title":"A high-level 3D visualization API for Java and ImageJ","volume":"11","author":"Schmid","year":"2010","journal-title":"BMC Bioinformatics"},{"key":"B38","doi-asserted-by":"publisher","first-page":"217","DOI":"10.1007\/s13346-016-0316-9","article-title":"Human barrier models for the in vitro assessment of drug delivery","volume":"7","author":"Schweinlin","year":"2017","journal-title":"Drug Deliv. Transl. Res."},{"key":"B39","doi-asserted-by":"publisher","first-page":"873","DOI":"10.1089\/ten.tec.2016.0101","article-title":"Development of an advanced primary human in vitro model of the small intestine","volume":"22","author":"Schweinlin","year":"2016","journal-title":"Tissue Eng. Part C Methods"},{"key":"B40","first-page":"173","article-title":"Biochemical and biomechanical characterization of porcine small intestinal submucosa (SIS): a mini review","volume":"3","author":"Shi","year":"2013","journal-title":"Int. J. Burns Trauma"},{"key":"B41","doi-asserted-by":"publisher","first-page":"1025","DOI":"10.1016\/j.ijpara.2007.11.002","article-title":"Transient transfection of Echinococcus multilocularis primary cells and complete in vitro regeneration of metacestode vesicles","volume":"38","author":"Spiliotis","year":"2008","journal-title":"Int. J. Parasitol."},{"key":"B42","doi-asserted-by":"publisher","first-page":"107","DOI":"10.1177\/2211068214561025","article-title":"TEER measurement techniques for in vitro barrier model systems","volume":"20","author":"Srinivasan","year":"2015","journal-title":"J. Lab. Autom."},{"key":"B43","doi-asserted-by":"publisher","first-page":"e0134342","DOI":"10.1371\/journal.pone.0134342","article-title":"Expression of opacity proteins interferes with the transmigration of Neisseria gonorrhoeae across polarized epithelial cells","volume":"10","author":"Stein","year":"2015","journal-title":"PLoS ONE"},{"key":"B44","doi-asserted-by":"publisher","first-page":"7355","DOI":"10.1016\/j.biomaterials.2014.05.031","article-title":"An engineered 3D human airway mucosa model based on an SIS scaffold","volume":"35","author":"Steinke","year":"2014","journal-title":"Biomaterials"},{"key":"B45","doi-asserted-by":"publisher","first-page":"e1006269","DOI":"10.1371\/journal.ppat.1006269","article-title":"Neisseria gonorrhoeae infects the human endocervix by activating non-muscle myosin II-mediated epithelial exfoliation","volume":"13","author":"Wang","year":"2017","journal-title":"PLoS Pathog."},{"key":"B46","doi-asserted-by":"publisher","first-page":"1448","DOI":"10.1038\/sj.ki.5002579","article-title":"Apical junctional complexes and cell polarity","volume":"72","author":"Wang","year":"2007","journal-title":"Kidney Int."},{"key":"B47","doi-asserted-by":"publisher","first-page":"e1002344","DOI":"10.1371\/journal.pmed.1002344","article-title":"Antimicrobial resistance in Neisseria gonorrhoeae: global surveillance and a call for international collaborative action","volume":"14","author":"Wi","year":"2017","journal-title":"PLoS Med."},{"key":"B48","doi-asserted-by":"publisher","first-page":"1317","DOI":"10.1016\/j.biomaterials.2010.10.006","article-title":"Human urine-derived stem cells seeded in a modified 3D porous small intestinal submucosa scaffold for urethral tissue engineering","volume":"32","author":"Wu","year":"2011","journal-title":"Biomaterials"}],"container-title":["Frontiers in Microbiology"],"original-title":[],"link":[{"URL":"https:\/\/www.frontiersin.org\/article\/10.3389\/fmicb.2019.01740\/full","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2021,1,15]],"date-time":"2021-01-15T16:06:02Z","timestamp":1610726762000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.frontiersin.org\/article\/10.3389\/fmicb.2019.01740\/full"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2019,7,31]]},"references-count":48,"alternative-id":["10.3389\/fmicb.2019.01740"],"URL":"https:\/\/doi.org\/10.3389\/fmicb.2019.01740","relation":{},"ISSN":["1664-302X"],"issn-type":[{"value":"1664-302X","type":"electronic"}],"subject":[],"published":{"date-parts":[[2019,7,31]]},"article-number":"1740"}}