{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,18]],"date-time":"2026-08-18T04:18:30Z","timestamp":1787026710244,"version":"build-2736575974"},"reference-count":16,"publisher":"Frontiers Media SA","license":[{"start":{"date-parts":[[2019,8,27]],"date-time":"2019-08-27T00:00:00Z","timestamp":1566864000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100005416","name":"Norges Forskningsr\u00e5d","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100005416","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["frontiersin.org"],"crossmark-restriction":true},"short-container-title":["Front. Microbiol."],"DOI":"10.3389\/fmicb.2019.01939","type":"journal-article","created":{"date-parts":[[2019,8,27]],"date-time":"2019-08-27T08:58:43Z","timestamp":1566896323000},"update-policy":"https:\/\/doi.org\/10.3389\/crossmark-policy","source":"Crossref","is-referenced-by-count":25,"title":["A New Protocol for Molecular Detection of Cyclospora cayetanensis as Contaminants of Berry Fruits"],"prefix":"10.3389","volume":"10","author":[{"given":"Tamirat T.","family":"Temesgen","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kristoffer R.","family":"Tysnes","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lucy J.","family":"Robertson","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1965","published-online":{"date-parts":[[2019,8,27]]},"reference":[{"key":"B1","doi-asserted-by":"publisher","first-page":"497","DOI":"10.1016\/j.fm.2018.07.013","article-title":"Evaluation of the U.S. food and drug administration validated method for detection of Cyclospora cayetanensis in high-risk fresh produce matrices and a method modification for a prepared dish.","volume":"76","author":"Almeria","year":"2018","journal-title":"Food Microbiol."},{"key":"B2","doi-asserted-by":"publisher","first-page":"1101","DOI":"10.15585\/mmwr.mm6739a6","article-title":"Notes from the field: multiple cyclosporiasis outbreaks-United States, 2018.","volume":"67","author":"Casillas","year":"2018","journal-title":"MMWR Morb. Mortal Wkly. Rep."},{"key":"B3","author":"Chacin-Bonilla","year":"2017","journal-title":"\u201c"},{"key":"B4","doi-asserted-by":"publisher","first-page":"1348","DOI":"10.4315\/0362-028X.JFP-13-381","article-title":"Efficacy of wash solutions in recovering Cyclospora cayetanensis, Cryptosporidium parvum, and Toxoplasma gondii from basil.","volume":"77","author":"Chandra","year":"2014","journal-title":"J. Food Prot."},{"key":"B5","doi-asserted-by":"publisher","first-page":"88","DOI":"10.1016\/j.exppara.2016.03.022","article-title":"Surface binding properties of aged and fresh (recently excreted) Toxoplasma gondii oocysts.","volume":"165","author":"Harito","year":"2016","journal-title":"Exp. Parasitol."},{"key":"B6","doi-asserted-by":"publisher","first-page":"103","DOI":"10.1590\/s0074-02762005000200001","article-title":"The genus Cyclospora (Apicomplexa: Eimeriidae), with a description of Cyclospora schneideri n.sp. in the snake Anilius scytale (Aniliidae) from Amazonian Brazil: a review.","volume":"100","author":"Lainson","year":"2005","journal-title":"Mem\u00f3rias do Instituto Oswaldo Cruz"},{"key":"B7","doi-asserted-by":"publisher","first-page":"4354","DOI":"10.1128\/AEM.00032-08","article-title":"Highly sensitive and specific PCR assay for reliable detection of Cyclospora cayetanensis oocysts.","volume":"74","author":"Lalonde","year":"2008","journal-title":"Appl. Environ. Microbiol."},{"key":"B8","doi-asserted-by":"publisher","first-page":"170","DOI":"10.1016\/j.fm.2017.08.008","article-title":"Interlaboratory validation of an improved method for detection of Cyclospora cayetanensis in produce using a real-time PCR assay.","volume":"69","author":"Murphy","year":"2018","journal-title":"Food Microbiol."},{"key":"B9","doi-asserted-by":"publisher","first-page":"1133","DOI":"10.4315\/0362-028X.JFP-16-492","article-title":"Evaluation of an improved U.S. Food and drug administration method for the detection of Cyclospora cayetanensis in produce using real-time PCR.","volume":"80","author":"Murphy","year":"2017","journal-title":"J. Food Prot."},{"key":"B10","doi-asserted-by":"publisher","first-page":"3","DOI":"10.1016\/S1386-6532(02)00197-X","article-title":"Clinical virology in real time.","volume":"25","author":"Niesters","year":"2002","journal-title":"J. Clin. Virol."},{"key":"B11","doi-asserted-by":"publisher","first-page":"1475","DOI":"10.1016\/S0020-7519(01)00283-1","article-title":"Sequence variability in the first internal transcribed spacer region within and among Cyclospora species is consistent with polyparasitism.","volume":"31","author":"Olivier","year":"2001","journal-title":"Int. J. Parasitol."},{"key":"B12","doi-asserted-by":"publisher","first-page":"914","DOI":"10.1645\/GE-2505.1","article-title":"Intragenomic sequence variation of the ITS-1 region within a single flow-cytometry\u2013counted Cyclospora cayetanensis Oocysts.","volume":"96","author":"Riner","year":"2010","journal-title":"J. Parasitol."},{"key":"B13","doi-asserted-by":"publisher","first-page":"1014","DOI":"10.1111\/j.1365-2672.2012.05384.x","article-title":"PCR inhibitors \u2013 occurrence, properties and removal.","volume":"113","author":"Schrader","year":"2012","journal-title":"J. Appl. Microbiol."},{"key":"B14","doi-asserted-by":"publisher","first-page":"51","DOI":"10.1016\/j.mimet.2012.11.001","article-title":"Assessment of three commercial DNA extraction kits and a laboratory-developed method for detecting Cryptosporidium and Cyclospora in raspberry wash, basil wash and pesto.","volume":"92","author":"Shields","year":"2013","journal-title":"J. Microbiol. Methods"},{"key":"B15","doi-asserted-by":"publisher","first-page":"277","DOI":"10.1016\/s0167-7012(03)00036-8","article-title":"Sensitivity of PCR detection of Cyclospora cayetanensis in raspberries, basil, and mesclun lettuce.","volume":"54","author":"Steele","year":"2003","journal-title":"J. Microbiol. Methods"},{"key":"B16","doi-asserted-by":"publisher","first-page":"199","DOI":"10.1078\/1438-4221-52","article-title":"Detection of Cyclospora cayetanensis in travellers returning from the tropics and subtropics using microscopy and real-time PCR.","volume":"293","author":"Verweij","year":"2003","journal-title":"Int. J. Med. Microbiol."}],"container-title":["Frontiers in Microbiology"],"original-title":[],"link":[{"URL":"https:\/\/www.frontiersin.org\/article\/10.3389\/fmicb.2019.01939\/full","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2019,9,23]],"date-time":"2019-09-23T18:40:02Z","timestamp":1569264002000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.frontiersin.org\/article\/10.3389\/fmicb.2019.01939\/full"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2019,8,27]]},"references-count":16,"alternative-id":["10.3389\/fmicb.2019.01939"],"URL":"https:\/\/doi.org\/10.3389\/fmicb.2019.01939","relation":{},"ISSN":["1664-302X"],"issn-type":[{"value":"1664-302X","type":"electronic"}],"subject":[],"published":{"date-parts":[[2019,8,27]]},"article-number":"1939"}}