{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,4]],"date-time":"2026-06-04T22:05:06Z","timestamp":1780610706021,"version":"3.54.1"},"reference-count":50,"publisher":"Frontiers Media SA","license":[{"start":{"date-parts":[[2020,1,31]],"date-time":"2020-01-31T00:00:00Z","timestamp":1580428800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100003995","name":"Natural Science Foundation of Anhui Province","doi-asserted-by":"publisher","award":["Grant No. KJ2015A330"],"award-info":[{"award-number":["Grant No. KJ2015A330"]}],"id":[{"id":"10.13039\/501100003995","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100014718","name":"Innovative Research Group Project of the National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["81471983"],"award-info":[{"award-number":["81471983"]}],"id":[{"id":"10.13039\/100014718","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["frontiersin.org"],"crossmark-restriction":true},"short-container-title":["Front. Microbiol."],"DOI":"10.3389\/fmicb.2019.03151","type":"journal-article","created":{"date-parts":[[2020,1,31]],"date-time":"2020-01-31T10:12:33Z","timestamp":1580465553000},"update-policy":"https:\/\/doi.org\/10.3389\/crossmark-policy","source":"Crossref","is-referenced-by-count":10,"title":["Toxoplasma gondii ROP16I Deletion: The Exacerbated Impact on Adverse Pregnant Outcomes in Mice"],"prefix":"10.3389","volume":"10","author":[{"given":"Wen","family":"Cui","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Cong","family":"Wang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Qingli","family":"Luo","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tian","family":"Xing","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jilong","family":"Shen","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wei","family":"Wang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1965","published-online":{"date-parts":[[2020,1,31]]},"reference":[{"key":"B1","doi-asserted-by":"publisher","first-page":"1853","DOI":"10.1095\/biolreprod66.6.1853","article-title":"Differential regulation and function of the Fas\/Fas ligand system in human trophoblast cells.","volume":"66","author":"Aschkenazi","year":"2002","journal-title":"Biol. Reprod."},{"key":"B2","doi-asserted-by":"publisher","first-page":"607","DOI":"10.1128\/mmbr.64.3.607-623.2000","article-title":"Lytic cycle of Toxoplasma gondii.","volume":"64","author":"Black","year":"2000","journal-title":"Microbiol. Mol. Biol. Rev."},{"key":"B3","doi-asserted-by":"publisher","first-page":"2071","DOI":"10.1084\/jem.20130103","article-title":"A Toxoplasma dense granule protein, GRA24, modulates the early immune response to infection by promoting a direct and sustained host p38 MAPK activation.","volume":"210","author":"Braun","year":"2013","journal-title":"J. Exp. Med."},{"key":"B4","doi-asserted-by":"publisher","DOI":"10.3389\/fimmu.2014.00606","article-title":"M1\/M2 macrophage polarity in normal and complicated pregnancy.","volume":"5","author":"Brown","year":"2014","journal-title":"Front. Immunol."},{"key":"B5","doi-asserted-by":"publisher","DOI":"10.1371\/journal.ppat.1002236","article-title":"Toxoplasma gondii rhoptry kinase ROP16 activates STAT3 and STAT6 resulting in cytokine inhibition and arginase-1-dependent growth control.","volume":"7","author":"Butcher","year":"2011","journal-title":"PLoS Pathog."},{"key":"B6","doi-asserted-by":"publisher","DOI":"10.1095\/biolreprod.112.099465","article-title":"Maternal decidual macrophages inhibit NK cell killing of invasive cytotrophoblasts during human pregnancy.","volume":"88","author":"Co","year":"2013","journal-title":"Biol. Reprod."},{"key":"B7","doi-asserted-by":"publisher","first-page":"309","DOI":"10.1182\/blood-2005-02-0666","article-title":"CD11c- and CD11b-expressing mouse leukocytes transport single Toxoplasma gondii tachyzoites to the brain.","volume":"107","author":"Courret","year":"2006","journal-title":"Blood"},{"key":"B8","doi-asserted-by":"publisher","first-page":"1","DOI":"10.1016\/j.vetpar.2009.02.026","article-title":"Toxoplasmosis in sheep\u2013the last 20 years.","volume":"163","author":"Dubey","year":"2009","journal-title":"Vet. Parasitol."},{"key":"B9","doi-asserted-by":"publisher","first-page":"1566","DOI":"10.1128\/JCM.39.4.1566-1570.2001","article-title":"Genotypic characterization of Toxoplasma gondii strains associated with human toxoplasmosis in Spain: direct analysis from clinical samples.","volume":"39","author":"Fuentes","year":"2001","journal-title":"J. Clin. Microbiol."},{"key":"B10","doi-asserted-by":"publisher","DOI":"10.3389\/fimmu.2017.00467","article-title":"Uterine natural killer cells: functional distinctions and influence on pregnancy in humans and mice.","volume":"8","author":"Gaynor","year":"2017","journal-title":"Front. Immunol."},{"key":"B11","doi-asserted-by":"publisher","first-page":"615","DOI":"10.1128\/CMR.00005-17","article-title":"Toxoplasma effectors targeting host signaling and transcription.","volume":"30","author":"Hakimi","year":"2017","journal-title":"Clin. Microbiol. Rev."},{"key":"B12","doi-asserted-by":"publisher","first-page":"1877","DOI":"10.1017\/S0031182009990941","article-title":"Evidence for high levels of vertical transmission in Toxoplasma gondii.","volume":"136","author":"Hide","year":"2009","journal-title":"Parasitology"},{"key":"B13","doi-asserted-by":"publisher","first-page":"766","DOI":"10.1038\/nrmicro2858","article-title":"Modulation of innate immunity by Toxoplasma gondii virulence effectors.","volume":"10","author":"Hunter","year":"2012","journal-title":"Nat. Rev. Microbiol."},{"key":"B14","doi-asserted-by":"publisher","first-page":"472","DOI":"10.1016\/j.chom.2011.04.015","article-title":"Toxoplasma polymorphic effectors determine macrophage polarization and intestinal inflammation.","volume":"9","author":"Jensen","year":"2011","journal-title":"Cell Host Microbe"},{"key":"B15","doi-asserted-by":"publisher","first-page":"425","DOI":"10.1111\/j.1600-0897.2008.00595.x","article-title":"Evolution of non-cytotoxic uterine natural killer cells.","volume":"59","author":"Kalkunte","year":"2008","journal-title":"Am. J. Reprod. Immunol."},{"key":"B16","doi-asserted-by":"publisher","first-page":"4178","DOI":"10.4049\/jimmunol.174.7.4178","article-title":"p38 MAPK autophosphorylation drives macrophage IL-12 production during intracellular infection.","volume":"174","author":"Kim","year":"2005","journal-title":"J. Immunol."},{"key":"B17","doi-asserted-by":"publisher","first-page":"210","DOI":"10.1016\/j.micpath.2015.10.002","article-title":"IL-10 regulate decidual Tregs apoptosis contributing to the abnormal pregnancy with Toxoplasma gondii infection.","volume":"89","author":"Lao","year":"2015","journal-title":"Microb. Pathog."},{"key":"B18","doi-asserted-by":"publisher","first-page":"11423","DOI":"10.1073\/pnas.0601438103","article-title":"Globalization and the population structure of Toxoplasma gondii.","volume":"103","author":"Lehmann","year":"2006","journal-title":"Proc. Natl. Acad. Sci. U.S.A."},{"key":"B19","doi-asserted-by":"publisher","first-page":"717","DOI":"10.1111\/j.1365-3024.2009.01122.x","article-title":"Dysregulation of macrophage signal transduction by Toxoplasma gondii: past progress and recent advances.","volume":"31","author":"Leng","year":"2009","journal-title":"Parasite Immunol."},{"key":"B20","doi-asserted-by":"publisher","DOI":"10.3389\/fimmu.2017.01013","article-title":"Decidual macrophage functional polarization during abnormal pregnancy due to Toxoplasma gondii: role for LILRB4.","volume":"8","author":"Li","year":"2017","journal-title":"Front. Immunol."},{"key":"B21","doi-asserted-by":"publisher","first-page":"444","DOI":"10.1046\/j.8755-8920.2003.00112.x","article-title":"Cytokine secretion patterns of NK cells and macrophages in early human pregnancy decidua and blood: implications for suppressor macrophages in decidua.","volume":"50","author":"Lidstrom","year":"2003","journal-title":"Am. J. Reprod. Immunol."},{"key":"B22","doi-asserted-by":"publisher","first-page":"3019","DOI":"10.1007\/s00436-013-3475-3","article-title":"Trophoblast apoptosis through polarization of macrophages induced by Chinese Toxoplasma gondii isolates with different virulence in pregnant mice.","volume":"112","author":"Liu","year":"2013","journal-title":"Parasitol Res."},{"key":"B23","doi-asserted-by":"publisher","first-page":"183","DOI":"10.1016\/j.micpath.2018.08.043","article-title":"Decidual macrophage M1 polarization contributes to adverse pregnancy induced by Toxoplasma gondii PRU strain infection.","volume":"124","author":"Liu","year":"2018","journal-title":"Microb. Pathog."},{"key":"B24","doi-asserted-by":"publisher","first-page":"1435","DOI":"10.1093\/infdis\/jiu265","article-title":"Adoptive transfer of Treg cells counters adverse effects of Toxoplasma gondii infection on pregnancy.","volume":"210","author":"Liu","year":"2014","journal-title":"J. Infect. Dis."},{"key":"B25","doi-asserted-by":"publisher","DOI":"10.1155\/2013\/709810","article-title":"HIV-associated central nervous system disease in patients admitted at the Douala General Hospital between 2004 and 2009: a retrospective study.","volume":"2013","author":"Luma","year":"2013","journal-title":"AIDS Res. Treat."},{"key":"B26","doi-asserted-by":"publisher","first-page":"487","DOI":"10.1016\/j.pt.2011.08.001","article-title":"Toxoplasma gondii effectors are master regulators of the inflammatory response.","volume":"27","author":"Melo","year":"2011","journal-title":"Trends Parasitol."},{"key":"B27","doi-asserted-by":"publisher","first-page":"1965","DOI":"10.1016\/S0140-6736(04)16412-X","article-title":"Toxoplasmosis.","volume":"363","author":"Montoya","year":"2004","journal-title":"Lancet"},{"key":"B28","doi-asserted-by":"publisher","first-page":"554","DOI":"10.1086\/590149","article-title":"Management of Toxoplasma gondii infection during pregnancy.","volume":"47","author":"Montoya","year":"2008","journal-title":"Clin. Infect. Dis."},{"key":"B29","doi-asserted-by":"publisher","DOI":"10.1186\/1477-7827-1-119","article-title":"Potential role of macrophages as immunoregulators of pregnancy.","volume":"1","author":"Mor","year":"2003","journal-title":"Reprod. Biol. Endocrinol."},{"key":"B30","doi-asserted-by":"publisher","first-page":"460","DOI":"10.1111\/j.1600-0897.2010.00813.x","article-title":"The contribution of macrophages to normal and pathological pregnancies.","volume":"63","author":"Nagamatsu","year":"2010","journal-title":"Am. J. Reprod. Immunol."},{"key":"B31","doi-asserted-by":"publisher","first-page":"39","DOI":"10.1080\/jmf.13.1.39.44","article-title":"Maternal serum of women with pre-eclampsia reduces trophoblast cell viability: evidence for an increased sensitivity to Fas-mediated apoptosis.","volume":"13","author":"Neale","year":"2003","journal-title":"J. Matern. Fetal Neonatal Med."},{"key":"B32","first-page":"1158","article-title":"Toxoplasmosis in HIV\/AIDS: a living legacy.","volume":"40","author":"Nissapatorn","year":"2009","journal-title":"Southeast Asian J. Trop. Med. Public Health"},{"key":"B33","doi-asserted-by":"publisher","first-page":"1895","DOI":"10.1017\/S0031182007000200","article-title":"Cellular and molecular physiopathology of congenital toxoplasmosis: the dual role of IFN-gamma.","volume":"134","author":"Pfaff","year":"2007","journal-title":"Parasitology"},{"key":"B34","doi-asserted-by":"publisher","first-page":"359","DOI":"10.1016\/j.meegid.2011.12.010","article-title":"Molecular genotyping of Toxoplasma gondii from Central and South America revealed high diversity within and between populations.","volume":"12","author":"Rajendran","year":"2012","journal-title":"Infect. Genet. Evol."},{"key":"B35","doi-asserted-by":"publisher","first-page":"1143","DOI":"10.1038\/labinvest.3780326","article-title":"Macrophage-induced apoptosis limits endovascular trophoblast invasion in the uterine wall of preeclamptic women.","volume":"81","author":"Reister","year":"2001","journal-title":"Lab. Invest."},{"key":"B36","doi-asserted-by":"publisher","first-page":"535","DOI":"10.1080\/08820130802191375","article-title":"The role of macrophages in utero-placental interactions during normal and pathological pregnancy.","volume":"37","author":"Renaud","year":"2008","journal-title":"Immunol. Invest."},{"key":"B37","doi-asserted-by":"publisher","first-page":"418","DOI":"10.1128\/IAI.05899-11","article-title":"Tissue barriers of the human placenta to infection with Toxoplasma gondii.","volume":"80","author":"Robbins","year":"2012","journal-title":"Infect. Immun."},{"key":"B38","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0214310","article-title":"Type I ROP16 regulates retinal inflammatory responses during ocular toxoplasmosis.","volume":"14","author":"Rochet","year":"2019","journal-title":"PLoS One"},{"key":"B39","doi-asserted-by":"publisher","first-page":"195","DOI":"10.1084\/jem.20100717","article-title":"Strain-specific activation of the NF-kappaB pathway by GRA15, a novel Toxoplasma gondii dense granule protein.","volume":"208","author":"Rosowski","year":"2011","journal-title":"J. Exp. Med."},{"key":"B40","doi-asserted-by":"publisher","first-page":"1780","DOI":"10.1126\/science.1133690","article-title":"Polymorphic secreted kinases are key virulence factors in toxoplasmosis.","volume":"314","author":"Saeij","year":"2006","journal-title":"Science"},{"key":"B41","doi-asserted-by":"publisher","first-page":"1535","DOI":"10.1093\/cid\/cit557","article-title":"Clinical features and outcomes in patients with disseminated toxoplasmosis admitted to intensive care: a multicenter study.","volume":"57","author":"Schmidt","year":"2013","journal-title":"Clin. Infect. Dis."},{"key":"B42","doi-asserted-by":"publisher","first-page":"453","DOI":"10.1017\/S0031182013001844","article-title":"Geographical patterns of Toxoplasma gondii genetic diversity revealed by multilocus PCR-RFLP genotyping.","volume":"141","author":"Shwab","year":"2014","journal-title":"Parasitology"},{"key":"B43","doi-asserted-by":"publisher","DOI":"10.3389\/fimmu.2018.01222","article-title":"Toxoplasma Chinese 1 strain of WH3\u0394rop16I\/III\/gra15II genetic background contributes to abnormal pregnant outcomes in murine model.","volume":"9","author":"Wang","year":"2018","journal-title":"Front. Immunol."},{"key":"B44","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0053483","article-title":"Genotypes andmouse virulence of Toxoplasma gondii isolates from animals and humans in China.","volume":"8","author":"Wang","year":"2013","journal-title":"PLoS One"},{"key":"B45","doi-asserted-by":"publisher","first-page":"703","DOI":"10.1007\/s10096-013-2017-3","article-title":"Do the placental barrier, parasite genotype and Toll-like receptor polymorphisms contribute to the course of primary infection with various Toxoplasma gondii genotypes in pregnant women?","volume":"33","author":"Wujcicka","year":"2014","journal-title":"Eur. J. Clin. Microbiol. Infect. Dis"},{"key":"B46","doi-asserted-by":"publisher","first-page":"51","DOI":"10.1016\/j.exppara.2015.04.008","article-title":"Toxoplasma gondii isolate with genotype Chinese 1 triggers trophoblast apoptosis through oxidative stress and mitochondrial dysfunction in mice.","volume":"154","author":"Xu","year":"2015","journal-title":"Exp. Parasitol."},{"key":"B47","doi-asserted-by":"publisher","first-page":"2747","DOI":"10.1084\/jem.20091703","article-title":"A single polymorphic amino acid on Toxoplasma gondii kinase ROP16 determines the direct and strain-specific activation of Stat3.","volume":"206","author":"Yamamoto","year":"2009","journal-title":"J. Exp. Med."},{"key":"B48","doi-asserted-by":"publisher","first-page":"109","DOI":"10.1038\/nri3598","article-title":"Innate immunity to Toxoplasma gondii infection.","volume":"14","author":"Yarovinsky","year":"2014","journal-title":"Nat. Rev. Immunol."},{"key":"B49","doi-asserted-by":"publisher","first-page":"112","DOI":"10.1111\/j.1600-0897.2011.01065.x","article-title":"The Treg\/Th17 imbalance in Toxoplasma gondii-infected pregnant mice.","volume":"67","author":"Zhang","year":"2012","journal-title":"Am. J. Reprod. Immunol."},{"key":"B50","doi-asserted-by":"publisher","DOI":"10.3389\/fmicb.2017.00901","article-title":"The effect of TGF-beta on Treg Cells in adverse pregnancy outcome upon Toxoplasma gondii infection.","volume":"8","author":"Zhao","year":"2017","journal-title":"Front. Microbiol."}],"container-title":["Frontiers in Microbiology"],"original-title":[],"link":[{"URL":"https:\/\/www.frontiersin.org\/article\/10.3389\/fmicb.2019.03151\/full","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2020,1,31]],"date-time":"2020-01-31T10:12:35Z","timestamp":1580465555000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.frontiersin.org\/article\/10.3389\/fmicb.2019.03151\/full"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2020,1,31]]},"references-count":50,"alternative-id":["10.3389\/fmicb.2019.03151"],"URL":"https:\/\/doi.org\/10.3389\/fmicb.2019.03151","relation":{},"ISSN":["1664-302X"],"issn-type":[{"value":"1664-302X","type":"electronic"}],"subject":[],"published":{"date-parts":[[2020,1,31]]},"article-number":"3151"}}