{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,26]],"date-time":"2026-09-26T13:04:23Z","timestamp":1790427863236,"version":"4.1.0"},"reference-count":44,"publisher":"Frontiers Media SA","license":[{"start":{"date-parts":[[2020,4,21]],"date-time":"2020-04-21T00:00:00Z","timestamp":1587427200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100009401","name":"Medizinischen Fakult\u00e4t, Ludwig-Maximilians-Universit\u00e4t M\u00fcnchen","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100009401","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["frontiersin.org"],"crossmark-restriction":true},"short-container-title":["Front. Microbiol."],"DOI":"10.3389\/fmicb.2020.00690","type":"journal-article","created":{"date-parts":[[2020,4,21]],"date-time":"2020-04-21T05:43:40Z","timestamp":1587447820000},"update-policy":"https:\/\/doi.org\/10.3389\/crossmark-policy","source":"Crossref","is-referenced-by-count":7,"title":["A Novel Rapid Sample Preparation Method for MALDI-TOF MS Permits Borrelia burgdorferi Sensu Lato Species and Isolate Differentiation"],"prefix":"10.3389","volume":"11","author":[{"given":"Anna-Cathrine","family":"Neumann-Cip","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Volker","family":"Fingerle","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gabriele","family":"Margos","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Reinhard K.","family":"Straubinger","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Evelyn","family":"Overzier","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sebastian","family":"Ulrich","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Andreas","family":"Wieser","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1965","published-online":{"date-parts":[[2020,4,21]]},"reference":[{"key":"B1","first-page":"521","article-title":"Isolation and cultivation of Lyme disease spirochetes.","volume":"57","author":"Barbour","year":"1984","journal-title":"The Yale journal of biology and medicine"},{"key":"B2","doi-asserted-by":"publisher","first-page":"1614","DOI":"10.1111\/j.1469-0691.2010.03311.x","article-title":"Matrix-assisted laser desorption ionization time-of-flight mass spectrometry, a revolution in clinical microbial identification.","volume":"16","author":"Bizzini","year":"2010","journal-title":"Clin Microbiol Infect"},{"key":"B3","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0185430","article-title":"Assessment of MALDI-TOF MS biotyping for Borrelia burgdorferi sl detection in Ixodes ricinus.","volume":"12","author":"Boyer","year":"2017","journal-title":"PLoS One"},{"key":"B4","doi-asserted-by":"publisher","DOI":"10.3389\/fmicb.2019.01923","article-title":"The RpoS Gatekeeper in Borrelia burgdorferi: An Invariant Regulatory Scheme That Promotes Spirochete Persistence in Reservoir Hosts and Niche Diversity.","volume":"10","author":"Caimano","year":"2019","journal-title":"Frontiers in Microbiology"},{"key":"B5","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0088895","article-title":"Identification of Borrelia species after creation of an in-house MALDI-TOF MS database.","volume":"9","author":"Calderaro","year":"2014","journal-title":"PLoS One"},{"key":"B6","doi-asserted-by":"publisher","DOI":"10.1186\/s12864-017-3553-5","article-title":"Plasmid diversity and phylogenetic consistency in the Lyme disease agent Borrelia burgdorferi.","volume":"18","author":"Casjens","year":"2017","journal-title":"BMC Genomics"},{"key":"B7","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0033280","article-title":"Genome stability of Lyme disease spirochetes: comparative genomics of Borrelia burgdorferi plasmids.","volume":"7","author":"Casjens","year":"2012","journal-title":"PLoS One"},{"key":"B8","doi-asserted-by":"publisher","first-page":"380","DOI":"10.1111\/j.1574-6976.2011.00298.x","article-title":"Applications of MALDI-TOF mass spectrometry in clinical diagnostic microbiology.","volume":"36","author":"Croxatto","year":"2012","journal-title":"FEMS Microbiology Reviews"},{"key":"B9","doi-asserted-by":"publisher","first-page":"59","DOI":"10.1007\/pl00008257","article-title":"Differential expression of outer surface proteins A and C by individual Borrelia burgdorferi in different genospecies.","volume":"189","author":"Fingerle","year":"2000","journal-title":"Med Microbiol Immunol"},{"key":"B10","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pntd.0002984","article-title":"MALDI-TOF mass spectrometry detection of pathogens in vectors: the Borrelia crocidurae\/Ornithodoros sonrai paradigm.","volume":"8","author":"Fotso Fotso","year":"2014","journal-title":"PLoS Negl Trop Dis"},{"key":"B11","doi-asserted-by":"publisher","first-page":"72","DOI":"10.1016\/j.meegid.2018.09.013","article-title":"Genome-wide analysis of Borrelia turcica and \u2018Candidatus Borrelia tachyglossi\u2019 shows relapsing fever-like genomes with unique genomic links to Lyme disease Borrelia.","volume":"66","author":"Gofton","year":"2018","journal-title":"Infect Genet Evol"},{"key":"B12","doi-asserted-by":"publisher","first-page":"1157","DOI":"10.1016\/j.ttbdis.2019.06.013","article-title":"First investigations on serum resistance and sensitivity of Borrelia turcica.","volume":"10","author":"Hepner","year":"2019","journal-title":"Ticks and Tick-borne Diseases"},{"key":"B13","doi-asserted-by":"publisher","first-page":"509","DOI":"10.1111\/mmi.12882","article-title":"Stage-specific global alterations in the transcriptomes of Lyme disease spirochetes during tick feeding and following mammalian host adaptation.","volume":"95","author":"Iyer","year":"2015","journal-title":"Molecular microbiology"},{"key":"B14","doi-asserted-by":"publisher","DOI":"10.1186\/s13071-016-1627-x","article-title":"Novel Borrelia species detected in echidna ticks, Bothriocroton concolor, in Australia.","volume":"9","author":"Loh","year":"2016","journal-title":"Parasites & Vectors"},{"key":"B15","doi-asserted-by":"publisher","first-page":"219","DOI":"10.1080\/10408363.2018.1450353","article-title":"Laboratory diagnosis of Lyme borreliosis: Current state of the art and future perspectives.","volume":"55","author":"Lohr","year":"2018","journal-title":"Crit Rev Clin Lab Sci"},{"key":"B16","doi-asserted-by":"publisher","first-page":"1952","DOI":"10.1017\/s0031182012000741","article-title":"Phylogeography of Lyme borreliosis-group spirochetes and methicillin-resistant Staphylococcus aureus.","volume":"139","author":"Margos","year":"2012","journal-title":"Parasitology"},{"key":"B17","doi-asserted-by":"publisher","DOI":"10.1016\/j.ttbdis.2019.101335","article-title":"Controversies in bacterial taxonomy: The example of the genus Borrelia.","volume":"11","author":"Margos","year":"2019","journal-title":"Ticks and Tick-borne Diseases"},{"key":"B18","doi-asserted-by":"publisher","first-page":"8730","DOI":"10.1073\/pnas.0800323105","article-title":"MLST of housekeeping genes captures geographic population structure and suggests a European origin of Borrelia burgdorferi.","volume":"105","author":"Margos","year":"2008","journal-title":"Proc Natl Acad Sci U S A"},{"key":"B19","doi-asserted-by":"publisher","first-page":"1545","DOI":"10.1016\/j.meegid.2011.07.022","article-title":"Population genetics, taxonomy, phylogeny and evolution of Borrelia burgdorferi sensu lato.","volume":"11","author":"Margos","year":"2011","journal-title":"Infect Genet Evol"},{"key":"B20","doi-asserted-by":"publisher","first-page":"191","DOI":"10.1128\/jb.57.2.191-206.1949","article-title":"A study of Borrelia Anserina infection (Spirochetosis) in Turkeys.","volume":"57","author":"McNeil","year":"1948","journal-title":"Journal of Bacteriology"},{"key":"B21","doi-asserted-by":"publisher","DOI":"10.1155\/2012\/595427","article-title":"Evaluating frequency, diagnostic quality, and cost of Lyme borreliosis testing in Germany: a retrospective model analysis.","volume":"2012","author":"Muller","year":"2012","journal-title":"Clin Dev Immunol"},{"key":"B22","doi-asserted-by":"publisher","first-page":"881","DOI":"10.1021\/acs.analchem.8b03646","article-title":"Identifying Dormant Growth State of Mycobacteria by Orthogonal Analytical Approaches on a Single Cell and Ensemble Basis.","volume":"91","author":"Neumann","year":"2019","journal-title":"Analytical Chemistry"},{"key":"B23","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.71.4.1689-1705.2003","article-title":"Profiling of Temperature-Induced Changes in Borrelia burgdorferi Gene Expression by Using Whole Genome Arrays.","volume":"71","author":"Ojaimi","year":"2003","journal-title":"Infection and Immunity"},{"key":"B24","doi-asserted-by":"publisher","first-page":"80","DOI":"10.1046\/j.1469-0691.2001.00200.x","article-title":"Tick-borne bacterial diseases emerging in Europe.","volume":"7","author":"Parola","year":"2001","journal-title":"Clin Microbiol Infect"},{"key":"B25","doi-asserted-by":"publisher","first-page":"S191","DOI":"10.1017\/s0031182003004694","article-title":"Lyme borreliosis in Europe and North America.","author":"Piesman","year":"2004","journal-title":"Parasitology"},{"key":"B26","doi-asserted-by":"publisher","first-page":"1251","DOI":"10.1128\/jcm.31.5.1251-1255.1993","article-title":"Standardization of medium for culturing Lyme disease spirochetes.","volume":"31","author":"Pollack","year":"1993","journal-title":"J Clin Microbiol"},{"key":"B27","doi-asserted-by":"publisher","first-page":"112","DOI":"10.1016\/s0176-6724(86)80110-9","article-title":"European Borrelia burgdorferi isolated from humans and ticks culture conditions and antibiotic susceptibility.","volume":"263","author":"Preac-Mursic","year":"1986","journal-title":"Zentralbl Bakteriol Mikrobiol Hyg A"},{"key":"B28","doi-asserted-by":"publisher","first-page":"556","DOI":"10.1016\/S1473-3099(15)00464-8","article-title":"Identification of a novel pathogenic Borrelia species causing Lyme borreliosis with unusually high spirochaetaemia: a descriptive study.","volume":"16","author":"Pritt","year":"2016","journal-title":"Lancet Infect Dis"},{"key":"B29","doi-asserted-by":"publisher","first-page":"87","DOI":"10.1038\/nrmicro2714","article-title":"Of ticks, mice and men: understanding the dual-host lifestyle of Lyme disease spirochaetes.","volume":"10","author":"Radolf","year":"2012","journal-title":"Nat Rev Microbiol"},{"key":"B30","doi-asserted-by":"publisher","DOI":"10.1186\/1471-2180-12-185","article-title":"Leptospira spp. strain identification by MALDI TOF MS is an equivalent tool to 16S rRNA gene sequencing and multi locus sequence typing (MLST).","volume":"12","author":"Rettinger","year":"2012","journal-title":"BMC Microbiol"},{"key":"B31","doi-asserted-by":"publisher","first-page":"1","DOI":"10.15585\/mmwr.ss6622a1","article-title":"Surveillance for Lyme Disease - United States, 2008-2015.","volume":"66","author":"Schwartz","year":"2017","journal-title":"MMWR Surveill Summ"},{"key":"B32","doi-asserted-by":"publisher","first-page":"543","DOI":"10.1086\/600885","article-title":"Ongoing Revolution in Bacteriology: Routine Identification of Bacteria by Matrix-Assisted Laser Desorption Ionization Time-of-Flight Mass Spectrometry.","volume":"49","author":"Seng","year":"2009","journal-title":"Clinical Infectious Diseases"},{"key":"B33","doi-asserted-by":"publisher","DOI":"10.3389\/fmicb.2015.00791","article-title":"MALDI-TOF mass spectrometry: an emerging technology for microbial identification and diagnosis.","volume":"6","author":"Singhal","year":"2015","journal-title":"Front Microbiol"},{"key":"B34","author":"Sohail","year":"2004","journal-title":"Bruker Daltonics MALDI Theory Mass Spectrometry, information brochure."},{"key":"B35","doi-asserted-by":"publisher","first-page":"450","DOI":"10.1097\/QCO.0b013e32832ee880","article-title":"Lyme borreliosis: a European perspective on diagnosis and clinical management.","volume":"22","author":"Stanek","year":"2009","journal-title":"Curr Opin Infect Dis"},{"key":"B36","doi-asserted-by":"publisher","first-page":"115","DOI":"10.1056\/nejm200107123450207","article-title":"Lyme disease.","volume":"345","author":"Steere","year":"2001","journal-title":"N Engl J Med"},{"key":"B37","doi-asserted-by":"publisher","first-page":"4535","DOI":"10.1128\/iai.63.11.4535-4539.1995","article-title":"Temperature-related differential expression of antigens in the Lyme disease spirochete, Borrelia burgdorferi.","volume":"63","author":"Stevenson","year":"1995","journal-title":"Infect Immun"},{"key":"B38","doi-asserted-by":"publisher","first-page":"2731","DOI":"10.1093\/molbev\/msr121","article-title":"MEGA5: molecular evolutionary genetics analysis using maximum likelihood, evolutionary distance, and maximum parsimony methods.","volume":"28","author":"Tamura","year":"2011","journal-title":"Mol Biol Evol"},{"key":"B39","doi-asserted-by":"publisher","first-page":"341","DOI":"10.1099\/jmm.0.000695","article-title":"MALDI-TOF mass spectrometry and high-consequence bacteria: safety and stability of biothreat bacterial sample testing in clinical diagnostic laboratories.","volume":"67","author":"Tracz","year":"2018","journal-title":"J Med Microbiol"},{"key":"B40","doi-asserted-by":"publisher","first-page":"7565","DOI":"10.1007\/s00216-016-9800-9","article-title":"Identification of Stachybotrys spp. by MALDI-TOF mass spectrometry.","volume":"408","author":"Ulrich","year":"2016","journal-title":"Analytical and bioanalytical chemistry"},{"key":"B41","doi-asserted-by":"publisher","first-page":"965","DOI":"10.1007\/s00253-011-3783-4","article-title":"MALDI-TOF MS in microbiological diagnostics-identification of microorganisms and beyond (mini review).","volume":"93","author":"Wieser","year":"2012","journal-title":"Appl Microbiol Biotechnol"},{"key":"B42","doi-asserted-by":"publisher","DOI":"10.1186\/s13071-014-0623-2","article-title":"RNA-Seq-based analysis of changes in Borrelia burgdorferi gene expression linked to pathogenicity.","volume":"8","author":"Wu","year":"2015","journal-title":"Parasites & Vectors"},{"key":"B43","doi-asserted-by":"publisher","first-page":"549","DOI":"10.2217\/fmb.16.5","article-title":"Emerging tools for identification of arthropod vectors.","volume":"11","author":"Yssouf","year":"2016","journal-title":"Future Microbiol"},{"key":"B44","doi-asserted-by":"publisher","first-page":"153","DOI":"10.1016\/j.cimid.2014.05.002","article-title":"Identification of flea species using MALDI-TOF\/MS.","volume":"37","author":"Yssouf","year":"2014","journal-title":"Comp Immunol Microbiol Infect Dis"}],"container-title":["Frontiers in Microbiology"],"original-title":[],"link":[{"URL":"https:\/\/www.frontiersin.org\/article\/10.3389\/fmicb.2020.00690\/full","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2020,4,21]],"date-time":"2020-04-21T05:43:42Z","timestamp":1587447822000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.frontiersin.org\/article\/10.3389\/fmicb.2020.00690\/full"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2020,4,21]]},"references-count":44,"alternative-id":["10.3389\/fmicb.2020.00690"],"URL":"https:\/\/doi.org\/10.3389\/fmicb.2020.00690","relation":{},"ISSN":["1664-302X"],"issn-type":[{"value":"1664-302X","type":"electronic"}],"subject":[],"published":{"date-parts":[[2020,4,21]]},"article-number":"690"}}