{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,14]],"date-time":"2026-10-14T21:12:57Z","timestamp":1792012377990,"version":"4.4.0"},"reference-count":61,"publisher":"Frontiers Media SA","license":[{"start":{"date-parts":[[2020,6,19]],"date-time":"2020-06-19T00:00:00Z","timestamp":1592524800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["frontiersin.org"],"crossmark-restriction":true},"short-container-title":["Front. Microbiol."],"DOI":"10.3389\/fmicb.2020.01375","type":"journal-article","created":{"date-parts":[[2020,6,19]],"date-time":"2020-06-19T03:24:01Z","timestamp":1592537041000},"update-policy":"https:\/\/doi.org\/10.3389\/crossmark-policy","source":"Crossref","is-referenced-by-count":47,"title":["Extensively-Drug Resistant Klebsiella pneumoniae Recovered From Neonatal Sepsis Cases From a Major NICU in Egypt"],"prefix":"10.3389","volume":"11","author":[{"given":"Noha A.","family":"Hassuna","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Reem A.","family":"AbdelAziz","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Amira","family":"Zakaria","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mohammed","family":"Abdelhakeem","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1965","published-online":{"date-parts":[[2020,6,19]]},"reference":[{"key":"B1","doi-asserted-by":"publisher","first-page":"685","DOI":"10.1038\/jp.2008.73","article-title":"Extended-spectrum \u03b2-lactamase producing Klebsiella pneumoniae in neonatal intensive care unit.","volume":"28","author":"Abdel-Hady","year":"2008","journal-title":"J. Perinatol."},{"key":"B2","doi-asserted-by":"publisher","first-page":"196","DOI":"10.1016\/j.jhin.2012.10.017","article-title":"Nosocomial infection surveillance in an Egyptian neonatal intensive care unit.","volume":"83","author":"Abdel-Wahab","year":"2013","journal-title":"J. Hosp. Infect."},{"key":"B3","doi-asserted-by":"publisher","first-page":"1647","DOI":"10.1007\/s10096-018-3294-7","article-title":"Carbapenem-resistant Gram-negative bacteria associated with catheter-related bloodstream infections in three intensive care units in Egypt.","volume":"37","author":"Abdulall","year":"2018","journal-title":"Eur. J. Clin. Microbiol. Infect. Dis."},{"key":"B4","doi-asserted-by":"crossref","first-page":"100","DOI":"10.4103\/ijabmr.IJABMR_333_16","article-title":"Dissemination of multidrug-resistant, class I and II integrons and molecular typing of CTX-M-producing Klebsiella pneumoniae.","volume":"8","author":"Akya","year":"2018","journal-title":"Int. J. Appl. Basic Med. Res."},{"key":"B5","doi-asserted-by":"publisher","DOI":"10.1186\/1476-0711-12-7","article-title":"Phenotypic and genotypic comparison of ESBL production by vaginal Escherichia coli isolates from pregnant and non-pregnant women.","volume":"12","author":"Al-Mayahie","year":"2013","journal-title":"Ann. Clin. Microbiol. Antimicrob."},{"key":"B6","first-page":"91","article-title":"Prevalence of extended spectrum Beta-Lactamase Genes among Escherichia coli and Klebsiella pneumoniae clinical isolates.","volume":"54","author":"Amer","year":"2020","journal-title":"Egypt. J. Microbiol."},{"key":"B7","doi-asserted-by":"crossref","DOI":"10.1186\/s13756-017-0208-x","article-title":"The threat of antimicrobial resistance in developing countries: causes and control strategies.","volume":"6","author":"Ayukekbong","year":"2017","journal-title":"Antimicrob. Resist. Infect. Control"},{"key":"B8","doi-asserted-by":"publisher","first-page":"979","DOI":"10.1093\/jac\/dkl077","article-title":"Molecular epidemiology of extended-spectrum \u03b2-lactamase-producing Klebsiella pneumoniae in a neonatal intensive care unit.","volume":"57","author":"Bagattini","year":"2006","journal-title":"J. Antimicrob. Chemother."},{"key":"B9","doi-asserted-by":"crossref","first-page":"16","DOI":"10.4103\/1755-6783.168709","article-title":"KPC with ESBL: a multistarrer tragedy.","volume":"9","author":"Banerjee","year":"2016","journal-title":"Ann. Trop. Med. Public Health"},{"key":"B10","doi-asserted-by":"publisher","DOI":"10.3389\/fmicb.2017.01230","article-title":"Extensively drug-resistant Klebsiella pneumoniae causing nosocomial bloodstream infections in China: molecular investigation of antibiotic resistance determinants, informing therapy, and clinical outcomes.","volume":"8","author":"Bi","year":"2017","journal-title":"Front. Microbiol."},{"key":"B11","doi-asserted-by":"publisher","first-page":"55","DOI":"10.1086\/421495","article-title":"Emergence of carbapenem-resistant Klebsiella species possessing the class A carbapenem-hydrolyzing KPC-2 and inhibitor-resistant TEM-30 beta-lactamases in New York City.","volume":"39","author":"Bradford","year":"2004","journal-title":"Clin. Infect. Dis."},{"key":"B12","author":"Branswell","year":"2017","journal-title":"). Who Releases list of World\u2019s Dangerous Superbugs."},{"key":"B13","doi-asserted-by":"publisher","first-page":"394","DOI":"10.1093\/jac\/dkm204","article-title":"Multiplex PCR for detection of plasmid-mediated quinolone resistance qnr genes in ESBL-producing enterobacterial isolates.","volume":"60","author":"Cattoir","year":"2007","journal-title":"J. Antimicrob. Chemother."},{"key":"B14","doi-asserted-by":"crossref","DOI":"10.5812\/jjm.14341","article-title":"Molecular characterization of carbapenem-resistant Klebsiella pneumoniae species isolated from a Tertiary Hospital, Ankara, Turkey.","volume":"10","author":"Celikbilek","year":"2017","journal-title":"Jundishapur J. Microbiol."},{"key":"B15","doi-asserted-by":"publisher","DOI":"10.1186\/1471-2180-10-27","article-title":"Molecular epidemiology of multidrug resistant extended spectrum beta-lactamase producing Klebsiella pneumoniae at a Jamaican hospital, 2000-2004.","volume":"10","author":"Christian","year":"2010","journal-title":"BMC Microbiol."},{"key":"B16","doi-asserted-by":"publisher","first-page":"382","DOI":"10.1038\/sj.jp.7211120","article-title":"Nosocomial infection in the NICU: a medical complication or unavoidable problem?","volume":"24","author":"Clark","year":"2004","journal-title":"J. Perinatol."},{"key":"B17","year":"2018","journal-title":"Performance Standards for Antimicrobial Susceptibility Testing."},{"key":"B18","doi-asserted-by":"crossref","first-page":"417","DOI":"10.1128\/MMBR.00016-10","article-title":"Origins and evolution of antibiotic resistance.","volume":"74","author":"Davies","year":"2010","journal-title":"Microbiol. Mol. Biol. Rev."},{"key":"B19","doi-asserted-by":"publisher","DOI":"10.7717\/peerj.2896","article-title":"Panel strain of Klebsiella pneumoniae for beta-lactam antibiotic evaluation: their phenotypic and genotypic characterization.","volume":"5","author":"Dsouza","year":"2017","journal-title":"PeerJ"},{"key":"B20","doi-asserted-by":"publisher","first-page":"1","DOI":"10.1155\/2015\/509484","article-title":"Epidemiology of neonatal sepsis and implicated pathogens: a study from Egypt.","volume":"2015","author":"El-Din","year":"2015","journal-title":"BioMed Res. Int."},{"key":"B21","doi-asserted-by":"publisher","first-page":"629","DOI":"10.1089\/sur.2018.088","article-title":"High prevalence of VIM, KPC, and NDM expression among surgical site infection pathogens in patients having emergency surgery.","volume":"19","author":"El-Kholy","year":"2018","journal-title":"Surg. Infect."},{"key":"B22","doi-asserted-by":"crossref","first-page":"332","DOI":"10.3345\/kjp.2013.56.8.332","article-title":"Early-onset sepsis in a neonatal intensive care unit in Beni Suef, Egypt: bacterial isolates and antibiotic resistance pattern.","volume":"56","author":"Fahmey","year":"2013","journal-title":"Korean J. Pediatr."},{"key":"B23","first-page":"25","article-title":"Antibiotics and bacterial resistance in the 21st century.","volume":"6","author":"Fair","year":"2014","journal-title":"Perspect. Med. Chem."},{"key":"B24","doi-asserted-by":"publisher","first-page":"2041","DOI":"10.20546\/ijcmas.2018.704.235","article-title":"Prevalence of carbapenem resistant klebsiella and proteus clinical isolates: a real threat to the egyptian health care system.","volume":"7","author":"Fanaki","year":"2018","journal-title":"Int. J. Curr. Microbiol. App. Sci."},{"key":"B25","doi-asserted-by":"crossref","first-page":"243","DOI":"10.1007\/s12199-015-0468-0","article-title":"Understanding the contribution of environmental factors in the spread of antimicrobial resistance.","volume":"20","author":"Fletcher","year":"2015","journal-title":"Environ. Health Prevent. Med."},{"key":"B26","doi-asserted-by":"publisher","first-page":"297","DOI":"10.1086\/505397","article-title":"Plasmid-mediated quinolone resistance in non-Typhi serotypes of Salmonella enterica.","volume":"43","author":"Gay","year":"2006","journal-title":"Clin. Infect. Dis."},{"key":"B27","doi-asserted-by":"crossref","first-page":"583","DOI":"10.1007\/s10096-019-03761-2","article-title":"Genetic diversity of carbapenem-resistant Klebsiella pneumoniae causing neonatal sepsis in intensive care unit, Cairo, Egypt.","volume":"39","author":"Ghaith","year":"2019","journal-title":"Eur. J. Clin. Microbiol. Infect. Dis."},{"key":"B28","doi-asserted-by":"publisher","first-page":"813","DOI":"10.1128\/aac.01169-07","article-title":"Clinical and economic impact of common multidrug-resistant gram-negative bacilli.","volume":"52","author":"Giske","year":"2008","journal-title":"Antimicrob. Agents Chemother."},{"key":"B29","doi-asserted-by":"publisher","first-page":"208","DOI":"10.1093\/cid\/cix270","article-title":"Gastrointestinal carriage is a major reservoir of Klebsiella pneumoniae infection in intensive care patients.","volume":"65","author":"Gorrie","year":"2017","journal-title":"Clin. Infect. Dis."},{"key":"B30","doi-asserted-by":"publisher","first-page":"1","DOI":"10.12816\/0046244","article-title":"Co-occurrence of plasmid-mediated quinolone resistance and carbapenemases in Klebsiella pneumoniae Isolates in Assiut. Egypt.","volume":"38","author":"Hammad","year":"2017","journal-title":"Egypt. J. Med. Microbiol."},{"key":"B31","doi-asserted-by":"crossref","first-page":"2720","DOI":"10.3390\/ijerph10072720","article-title":"Optimization of pulse-field gel electrophoresis for subtyping of Klebsiella pneumoniae.","volume":"10","author":"Han","year":"2013","journal-title":"Int. J. Environ. Res. Public Health"},{"key":"B32","doi-asserted-by":"publisher","first-page":"1677","DOI":"10.1093\/cid\/cis275","article-title":"Unmet medical need in infectious diseases.","volume":"54","author":"Hersh","year":"2012","journal-title":"Clin. Infect. Dis."},{"key":"B33","doi-asserted-by":"publisher","first-page":"2039","DOI":"10.1093\/jac\/dky164","article-title":"Emergence of an XDR and carbapenemase-producing hypervirulent Klebsiella pneumoniae strain in Taiwan.","volume":"73","author":"Huang","year":"2018","journal-title":"J. Antimicrob. Chemother."},{"key":"B34","doi-asserted-by":"publisher","first-page":"1260","DOI":"10.1093\/jac\/dkr135","article-title":"NDM-2 carbapenemase in Acinetobacter baumannii from Egypt.","volume":"66","author":"Kaase","year":"2011","journal-title":"J. Antimicrob. Chemother."},{"key":"B35","first-page":"101","article-title":"Detection of carbapenemase encoding genes in Enterobacteriace, Pseudomonas aeruginosa, and Acinetobacter baumanii isolated from patients at Intensive Care Unit Cipto Mangunkusumo Hospital in 2011.","volume":"45","author":"Karuniawati","year":"2013","journal-title":"Acta Med. Indones"},{"key":"B36","doi-asserted-by":"publisher","DOI":"10.3389\/fmed.2018.00225","article-title":"Virulence factors and antibiotic resistance of Klebsiella pneumoniae strains isolated from neonates with sepsis.","volume":"5","author":"Khaertynov","year":"2018","journal-title":"Front. Med."},{"key":"B37","doi-asserted-by":"crossref","DOI":"10.1093\/ofid\/ofy074","article-title":"Case report of an extensively drug-resistant Klebsiella pneumoniae infection with genomic characterization of the strain and review of similar cases in the United States.","volume":"5","author":"Krapp","year":"2018","journal-title":"Open Forum Infect. Dis."},{"key":"B38","doi-asserted-by":"publisher","first-page":"268","DOI":"10.1111\/j.1469-0691.2011.03570.x","article-title":"Multidrug-resistant, extensively drug-resistant and pandrug-resistant bacteria: an international expert proposal for interim standard definitions for acquired resistance.","volume":"18","author":"Magiorakos","year":"2012","journal-title":"Clin. Microbiol. Infect."},{"key":"B39","doi-asserted-by":"publisher","first-page":"e1","DOI":"10.1016\/j.redar.2013.11.012","article-title":"Bugs, hosts and ICU environment: countering pan-resistance in nosocomial microbiota and treating bacterial infections in the critical care setting.","volume":"61","author":"Maseda","year":"2014","journal-title":"Rev. Espanola Anestesiol. Reanimac."},{"key":"B40","article-title":"Detection of Klebsiella pneumoniae carbapenemases and metallo-\u03b2-lactamases among Klebsiella pneumoniae isolates from hospitalized patients at Menoufia University Hospitals.","volume":"29","author":"Melake","year":"2016","journal-title":"Egypt. Menoufia Med. J."},{"key":"B41","doi-asserted-by":"crossref","DOI":"10.1186\/s13756-019-0477-7","article-title":"Evaluation of co-transfer of plasmid-mediated fluoroquinolone resistance genes and blaNDM gene in Enterobacteriaceae causing neonatal septicaemia.","volume":"8","author":"Mitra","year":"2019","journal-title":"Antimicrob. Resist. Infect. Control"},{"key":"B42","doi-asserted-by":"publisher","first-page":"628","DOI":"10.1099\/jmm.0.000473","article-title":"Epidemiological typing of multidrug-resistant Klebsiella pneumoniae, which causes paediatric ventilator-associated pneumonia in Egypt.","volume":"66","author":"Mohamed","year":"2017","journal-title":"J. Med. Microbiol."},{"key":"B43","first-page":"1","article-title":"Prevalence of Carbapenemases among Klebsiella pneumoniae Strains Isolated from Respiratory Tract Specimens.","volume":"38","author":"Mohamed","year":"2016","journal-title":"Egypt. J. Med. Microbiol."},{"key":"B44","doi-asserted-by":"crossref","DOI":"10.1186\/s13756-017-0225-9","article-title":"Emerging antimicrobial resistance in early and late-onset neonatal sepsis.","volume":"6","author":"Mohsen","year":"2017","journal-title":"Antimicrob. Resist. Infect. Control"},{"key":"B45","doi-asserted-by":"publisher","first-page":"590","DOI":"10.1097\/01.inf.0000168804.09875.95","article-title":"Neonatal sepsis in Egypt associated with bacterial contamination of glucose-containing intravenous fluids.","volume":"24","author":"Moore","year":"2005","journal-title":"Pediatr. Infect. Dis. J."},{"key":"B46","first-page":"33","article-title":"Neonatal sepsis: causative bacteria and their resistance to antibiotics.","volume":"22","author":"Muhammad","year":"2010","journal-title":"J. Ayub Med. Coll. Abbott."},{"key":"B47","doi-asserted-by":"crossref","first-page":"4943","DOI":"10.1128\/AAC.00296-11","article-title":"Carbapenems: past, present, and future.","volume":"55","author":"Papp-Wallace","year":"2011","journal-title":"Antimicrob. Agents Chemother."},{"key":"B48","doi-asserted-by":"publisher","first-page":"11","DOI":"10.1177\/2049936117727497","article-title":"Risk factors and outcomes for multidrug-resistant Gram-negative bacilli bacteremia.","volume":"5","author":"Patolia","year":"2018","journal-title":"Ther. Adv. Infect. Dis."},{"key":"B49","doi-asserted-by":"publisher","first-page":"13","DOI":"10.1016\/j.drup.2016.09.001","article-title":"Plasmid-mediated quinolone resistance: two decades on.","volume":"29","author":"Rodr\u00edguez-Mart\u00ednez","year":"2016","journal-title":"Drug Resist. Updates"},{"key":"B50","doi-asserted-by":"publisher","first-page":"675","DOI":"10.1055\/s-0034-1393936","article-title":"Risk factors and outcomes of late-onset bacterial sepsis in preterm neonates born at < 32 weeks\u2019 gestation.","volume":"32","author":"Shah","year":"2015","journal-title":"Am. J. Perinatol."},{"key":"B51","doi-asserted-by":"crossref","first-page":"192","DOI":"10.4103\/ijmr.IJMR_2092_17","article-title":"Plasmid-mediated fluoroquinolone resistance associated with extra-intestinal Escherichia coli isolates from hospital samples.","volume":"149","author":"Shetty","year":"2019","journal-title":"Indian J. Med. Res."},{"key":"B52","doi-asserted-by":"publisher","first-page":"5407","DOI":"10.1128\/jcm.41.12.5407-5413.2003","article-title":"PCR typing of genetic determinants for Metallo-\u03b2-Lactamases and integrases carried by gram-negative bacteria Isolated in Japan, with Focus on the Class 3 Integron.","volume":"41","author":"Shibata","year":"2003","journal-title":"J. Clin. Microbiol."},{"key":"B53","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0116421","article-title":"Molecular characterization of Klebsiella pneumoniae carbapenemase (Kpc)-producing Enterobacteriaceae in Ontario.","volume":"9","author":"Tijet","year":"2014","journal-title":"PLoS One"},{"key":"B54","doi-asserted-by":"publisher","first-page":"1290","DOI":"10.1128\/jcm.38.3.1290-1292.2000","article-title":"Outbreak of infections caused by Pseudomonas aeruginosa producing VIM-1 carbapenemase in Greece.","volume":"38","author":"Tsakris","year":"2000","journal-title":"J. Clin. Microbiol."},{"key":"B55","doi-asserted-by":"publisher","first-page":"577","DOI":"10.1089\/mdr.2014.0226","article-title":"The role of OmpK35, OmpK36 porins, and production of \u03b2-lactamases on imipenem susceptibility in Klebsiella pneumoniae clinical isolates, Cairo, Egypt.","volume":"21","author":"Wassef","year":"2015","journal-title":"Microb. Drug Resist."},{"key":"B56","doi-asserted-by":"publisher","first-page":"525","DOI":"10.1016\/j.mib.2005.08.016","article-title":"\u03b2-Lactam antibiotic resistance: a current structural perspective.","volume":"8","author":"Wilke","year":"2005","journal-title":"Curr. Opin. Microbiol."},{"key":"B57","year":"2017","journal-title":"Antimicrobial Resistance: Global Report on Surveillance."},{"key":"B58","doi-asserted-by":"publisher","first-page":"135","DOI":"10.1016\/s0140-6736(08)60106-3","article-title":"Clinical signs that predict severe illness in children under age 2 months: a multicentre study.","volume":"371","year":"2008","journal-title":"Lancet"},{"key":"B59","doi-asserted-by":"publisher","first-page":"1787","DOI":"10.1099\/jmm.0.001093","article-title":"Modified PFGE protocol for improving typeability of DNA degradation susceptible nosocomial Klebsiella pneumoniae.","volume":"68","author":"Zakaria","year":"2019","journal-title":"J. Med. Microbiol."},{"key":"B60","doi-asserted-by":"publisher","first-page":"1080","DOI":"10.1099\/jmm.0.010017-0","article-title":"Relevance of resistance levels to carbapenems and integron-borne blaIMP-1, blaIMP-7, blaIMP-10 and blaVIM-2 in clinical isolates of Pseudomonas aeruginosa.","volume":"58","author":"Zhao","year":"2009","journal-title":"J. Med. Microbiol."},{"key":"B61","doi-asserted-by":"publisher","DOI":"10.3389\/fmicb.2017.00371","article-title":"Defining and evaluating a core genome multilocus sequence typing scheme for whole-genome sequence-based typing of Klebsiella pneumoniae.","volume":"8","author":"Zhou","year":"2017","journal-title":"Front. Microbiol."}],"container-title":["Frontiers in Microbiology"],"original-title":[],"link":[{"URL":"https:\/\/www.frontiersin.org\/article\/10.3389\/fmicb.2020.01375\/full","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2021,3,20]],"date-time":"2021-03-20T21:54:58Z","timestamp":1616277298000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.frontiersin.org\/article\/10.3389\/fmicb.2020.01375\/full"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2020,6,19]]},"references-count":61,"alternative-id":["10.3389\/fmicb.2020.01375"],"URL":"https:\/\/doi.org\/10.3389\/fmicb.2020.01375","relation":{},"ISSN":["1664-302X"],"issn-type":[{"value":"1664-302X","type":"electronic"}],"subject":[],"published":{"date-parts":[[2020,6,19]]},"article-number":"1375"}}