{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,13]],"date-time":"2026-05-13T04:43:53Z","timestamp":1778647433966,"version":"3.51.4"},"reference-count":55,"publisher":"Frontiers Media SA","license":[{"start":{"date-parts":[[2020,7,3]],"date-time":"2020-07-03T00:00:00Z","timestamp":1593734400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/100000002","name":"National Institutes of Health","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["frontiersin.org"],"crossmark-restriction":true},"short-container-title":["Front. Microbiol."],"DOI":"10.3389\/fmicb.2020.01496","type":"journal-article","created":{"date-parts":[[2020,7,3]],"date-time":"2020-07-03T08:15:26Z","timestamp":1593764126000},"update-policy":"https:\/\/doi.org\/10.3389\/crossmark-policy","source":"Crossref","is-referenced-by-count":9,"title":["In vivo Characterization of Plasmodium berghei P47 (Pbs47) as a Malaria Transmission-Blocking Vaccine Target"],"prefix":"10.3389","volume":"11","author":[{"given":"Lampouguin","family":"Yenkoidiok-Douti","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Gaspar E.","family":"Canepa","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ana Beatriz F.","family":"Barletta","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Carolina","family":"Barillas-Mury","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1965","published-online":{"date-parts":[[2020,7,3]]},"reference":[{"key":"B1","doi-asserted-by":"publisher","first-page":"984","DOI":"10.1126\/science.1235264","article-title":"The human malaria parasite Pfs47 gene mediates evasion of the mosquito mmune system.","volume":"340","author":"Alvaro","year":"2013","journal-title":"Science"},{"key":"B2","doi-asserted-by":"publisher","first-page":"409","DOI":"10.1016\/j.ijpara.2016.10.002","article-title":"The s48\/45 six-cysteine proteins: mediators of interaction throughout the Plasmodium life cycle.","volume":"47","author":"Arredondo","year":"2017","journal-title":"Int. J. Parasitol."},{"key":"B3","doi-asserted-by":"publisher","first-page":"1","DOI":"10.1038\/ncomms2840","article-title":"Transmission-blocking interventions eliminate malaria from laboratory populations.","volume":"4","author":"Blagborough","year":"2013","journal-title":"Nat. Commun."},{"key":"B4","doi-asserted-by":"publisher","first-page":"3252","DOI":"10.1016\/j.vaccine.2016.05.007","article-title":"Transmission blocking potency and immunogenicity of a plant-produced Pvs25-based subunit vaccine against Plasmodium vivax.","volume":"34","author":"Blagborough","year":"2016","journal-title":"Vaccine"},{"key":"B5","doi-asserted-by":"publisher","DOI":"10.1038\/srep19234","article-title":"Plug-and-display: decoration of virus-like particles via isopeptide bonds for modular immunization.","volume":"6","author":"Brune","year":"2016","journal-title":"Sci. Rep."},{"key":"B6","doi-asserted-by":"publisher","DOI":"10.1038\/s41541-018-0065-5","article-title":"Antibody targeting of a specific region of Pfs47 blocks Plasmodium falciparum malaria transmission.","volume":"3","author":"Canepa","year":"2018","journal-title":"NPJ Vaccines"},{"key":"B7","doi-asserted-by":"publisher","DOI":"10.1128\/mBio.01627-18","article-title":"crossm transmission stages of Plasmodium vivax (Human Malaria Parasite) and P. berghei (Murine Malaria Parasite).","author":"Cao","year":"2018"},{"key":"B8","doi-asserted-by":"publisher","first-page":"5865","DOI":"10.1016\/j.vaccine.2018.08.033","article-title":"Safety and immunogenicity of a plant-produced Pfs25 virus-like particle as a transmission blocking vaccine against malaria: a Phase 1 dose-escalation study in healthy adults.","volume":"36","author":"Chichester","year":"2018","journal-title":"Vaccine"},{"key":"B9","doi-asserted-by":"publisher","first-page":"1199","DOI":"10.1038\/s41590-018-0228-6","article-title":"Malaria prevention: from immunological concepts to effective vaccines and protective antibodies.","volume":"19","author":"Cockburn","year":"2018","journal-title":"Nat. Immunol."},{"key":"B10","doi-asserted-by":"publisher","first-page":"1285","DOI":"10.1038\/sj.embor.7400831","article-title":"Anopheles and Plasmodium: from laboratory models to natural systems in the field.","volume":"7","author":"Cohuet","year":"2006","journal-title":"EMBO Rep."},{"key":"B11","doi-asserted-by":"publisher","DOI":"10.1371\/journal.ppat.1002401","article-title":"The role of animal models for research on severe malaria.","volume":"8","author":"Craig","year":"2012","journal-title":"PLoS Pathog."},{"key":"B12","doi-asserted-by":"publisher","first-page":"190","DOI":"10.1111\/imr.12828","article-title":"Immunity against sexual stage Plasmodium falciparum and Plasmodium vivax parasites.","volume":"293","author":"de Jong","year":"2020","journal-title":"Immunol. Rev."},{"key":"B13","doi-asserted-by":"publisher","DOI":"10.1371\/journal.ppat.0020052","article-title":"Anopheles gambiae immune responses to human and rodent Plasmodium parasite species.","volume":"2","author":"Dong","year":"2006","journal-title":"PLoS Pathog."},{"key":"B14","doi-asserted-by":"publisher","first-page":"44","DOI":"10.1016\/j.pep.2015.09.021","article-title":"Rapid analysis of protein expression and solubility with the SpyTag-SpyCatcher system.","volume":"117","author":"Dovala","year":"2016","journal-title":"Protein. Expr. Purif."},{"key":"B15","doi-asserted-by":"publisher","first-page":"43","DOI":"10.1016\/j.chom.2018.06.008","article-title":"Malaria vaccines: recent advances and new horizons.","volume":"24","author":"Draper","year":"2018","journal-title":"Cell Host Microbe"},{"key":"B16","doi-asserted-by":"publisher","first-page":"44","DOI":"10.1016\/j.coviro.2016.03.001","article-title":"Engineering virus-like particles as vaccine platforms.","volume":"18","author":"Frietze","year":"2016","journal-title":"Curr. Opin. Virol."},{"key":"B17","doi-asserted-by":"publisher","first-page":"6405","DOI":"10.1016\/j.vaccine.2019.09.004","article-title":"Virus-like particle-display of the enterotoxigenic Escherichia coli heat-stable toxoid STh-A14T elicits neutralizing antibodies in mice.","volume":"37","author":"Govasli","year":"2019","journal-title":"Vaccine"},{"key":"B18","doi-asserted-by":"publisher","first-page":"695.e4","DOI":"10.1016\/j.immuni.2018.08.012","article-title":"B cells are the dominant antigen-presenting cells that activate naive CD4+ T cells upon immunization with a virus-derived nanoparticle antigen.","volume":"49","author":"Hong","year":"2018","journal-title":"Immunity"},{"key":"B19","doi-asserted-by":"publisher","first-page":"71","DOI":"10.1006\/jsbi.1996.0013","article-title":"Computer visualization of three-dimensional image data using IMOD.","volume":"116","author":"Kremer","year":"1996","journal-title":"J. Struct. Biol."},{"key":"B20","doi-asserted-by":"publisher","DOI":"10.1186\/1745-7580-2-2","article-title":"Improved method for predicting linear B-cell epitopes.","volume":"2","author":"Larsen","year":"2006","journal-title":"Immunome Res."},{"key":"B21","doi-asserted-by":"publisher","first-page":"1","DOI":"10.1186\/s12936-016-1464-6","article-title":"Assessment of Pfs25 expressed from multiple soluble expression platforms for use as transmission-blocking vaccine candidates.","volume":"15","author":"Lee","year":"2016","journal-title":"Malar J."},{"key":"B22","doi-asserted-by":"publisher","first-page":"1","DOI":"10.1038\/s41598-017-03798-3","article-title":"Nanoassembly routes stimulate conflicting antibody quantity and quality for transmission-blocking malaria vaccines.","volume":"7","author":"Leneghan","year":"2017","journal-title":"Sci. Rep."},{"key":"B23","doi-asserted-by":"publisher","first-page":"1","DOI":"10.1038\/s41467-018-06340-9","article-title":"Structural basis for recognition of the malaria vaccine candidate Pfs48\/45 by a transmission blocking antibody.","volume":"9","author":"Lennartz","year":"2018","journal-title":"Nat. Commun."},{"key":"B24","doi-asserted-by":"publisher","first-page":"309","DOI":"10.1016\/j.jmb.2013.10.021","article-title":"Structural analysis and optimization of the covalent association between SpyCatcher and a peptide tag.","volume":"426","author":"Li","year":"2014","journal-title":"J. Mol. Biol."},{"key":"B25","doi-asserted-by":"publisher","first-page":"19913","DOI":"10.1074\/jbc.M116.732305","article-title":"Structural and immunological characterization of recombinant 6-cysteine domains of the Plasmodium falciparum sexual stage protein Pfs230.","volume":"291","author":"MacDonald","year":"2016","journal-title":"J. Biol. Chem."},{"key":"B26","doi-asserted-by":"publisher","first-page":"1","DOI":"10.1038\/s41598-018-33236-x","article-title":"The Plasmodium falciparum male gametocyte protein P230p, a paralog of P230, is vital for ookinete formation and mosquito transmission.","volume":"8","author":"Marin-Mogollon","year":"2018","journal-title":"Sci. Rep."},{"key":"B27","doi-asserted-by":"publisher","DOI":"10.1038\/s41467-019-11980-6","article-title":"Potent antibody lineage against malaria transmission elicited by human vaccination with Pfs25.","volume":"10","author":"McLeod","year":"2019","journal-title":"Nat. Commun."},{"key":"B28","doi-asserted-by":"publisher","DOI":"10.3389\/fimmu.2017.01998","article-title":"Assessment of antibodies induced by multivalent transmission-blocking malaria vaccines.","volume":"8","author":"Menon","year":"2018","journal-title":"Front. Immunol."},{"key":"B29","doi-asserted-by":"publisher","first-page":"1","DOI":"10.1186\/1475-2875-6-107","article-title":"Transmission-blocking activity induced by malaria vaccine candidates Pfs25\/Pvs25 is a direct and predictable function of antibody titer.","volume":"6","author":"Miura","year":"2007","journal-title":"Malar J."},{"key":"B30","doi-asserted-by":"publisher","first-page":"168","DOI":"10.1016\/j.mib.2017.11.029","article-title":"Plasmodium P47: a key gene for malaria transmission by mosquito vectors.","volume":"40","author":"Molina-Cruz","year":"2017","journal-title":"Curr. Opin. Microbiol."},{"key":"B31","doi-asserted-by":"publisher","DOI":"10.1186\/s12915-014-0086-0","article-title":"A comprehensive evaluation of rodent malaria parasite genomes and gene expression.","volume":"12","author":"Otto","year":"2014","journal-title":"BMC Med."},{"key":"B32","doi-asserted-by":"publisher","DOI":"10.1128\/IAI00486-17","article-title":"Evaluation of a Plasmodium-specific carier protein to enhance production of recombinant Pf s25 a leading transmission blocking vaccine candidate.","volume":"86","author":"Parzych","year":"2018","journal-title":"Infect. Immun."},{"key":"B33","doi-asserted-by":"publisher","DOI":"10.1038\/nrdp.2017.50","article-title":"Malaria.","volume":"3","author":"Phillips","year":"2017","journal-title":"Nat. Rev. Dis. Prim."},{"key":"B34","doi-asserted-by":"publisher","first-page":"94","DOI":"10.1016\/j.cbpa.2015.10.002","article-title":"Secrets of a covalent interaction for biomaterials and biotechnology: SpyTag and SpyCatcher.","volume":"29","author":"Reddington","year":"2015","journal-title":"Curr. Opin. Chem. Biol."},{"key":"B35","doi-asserted-by":"publisher","first-page":"969","DOI":"10.1016\/S1473-3099(18)30344-X","article-title":"Safety and immunogenicity of Pfs25H-EPA\/Alhydrogel, a transmission-blocking vaccine against Plasmodium falciparum: a randomised, double-blind, comparator-controlled, dose-escalation study in healthy malian adults.","volume":"18","author":"Sagara","year":"2018","journal-title":"Lancet Infect. Dis."},{"key":"B36","doi-asserted-by":"publisher","first-page":"7476","DOI":"10.1016\/j.vaccine.2015.08.073","article-title":"Transmission blocking malaria vaccines: assays and candidates in clinical development.","volume":"33","author":"Sauerwein","year":"2015","journal-title":"Vaccine"},{"key":"B37","doi-asserted-by":"publisher","DOI":"10.1038\/s41541-019-0121-9","article-title":"Outer membrane protein complex as a carrier for malaria transmission blocking antigen Pfs230.","volume":"4","author":"Scaria","year":"2019","journal-title":"NPJ Vaccines"},{"key":"B38","doi-asserted-by":"publisher","first-page":"90","DOI":"10.1016\/j.ijpddr.2017.01.004","article-title":"Blocking transmission of vector-borne diseases.","volume":"7","author":"Schorderet-Weber","year":"2017","journal-title":"Int. J. Parasitol. Drugs. Drug. Resist."},{"key":"B39","doi-asserted-by":"publisher","DOI":"10.3389\/fimmu.2019.01256","article-title":"Pfs230 and Pfs48\/45 fusion proteins elicit strong transmission-blocking antibody responses against Plasmodium falciparum.","volume":"10","author":"Singh","year":"2019","journal-title":"Front. Immunol."},{"key":"B40","doi-asserted-by":"publisher","first-page":"3726","DOI":"10.1016\/j.vaccine.2017.05.054","article-title":"Improving the malaria transmission-blocking activity of a Plasmodium falciparum 48\/45 based vaccine antigen by SpyTag\/SpyCatcher mediated virus-like display.","volume":"35","author":"Singh","year":"2017","journal-title":"Vaccine"},{"key":"B41","doi-asserted-by":"publisher","first-page":"644","DOI":"10.1590\/0074-0276130597","article-title":"The Plasmodium bottleneck: malaria parasite losses in the mosquito vector.","volume":"109","author":"Smith","year":"2014","journal-title":"Mem. Inst. Oswaldo. Cruz."},{"key":"B42","year":"2020","journal-title":"How Much Blood can I take from a Mouse without Endangering its Health?"},{"key":"B43","doi-asserted-by":"publisher","first-page":"2623","DOI":"10.1016\/j.vaccine.2014.03.020","article-title":"A multi-stage malaria vaccine candidate targeting both transmission and asexual parasite life-cycle stages.","volume":"32","author":"Theisen","year":"2014","journal-title":"Vaccine"},{"key":"B44","doi-asserted-by":"publisher","first-page":"1","DOI":"10.1172\/jci.insight.120692","article-title":"TLR-adjuvanted nanoparticle vaccines differentially influence the quality and longevity of responses to malaria antigen Pfs25.","volume":"3","author":"Thompson","year":"2018","journal-title":"JCI Insight"},{"key":"B45","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0009809","article-title":"Versatile virus-like particle carrier for epitope based vaccines.","volume":"5","author":"Tissot","year":"2010","journal-title":"PLoS One"},{"key":"B46","doi-asserted-by":"publisher","DOI":"10.1038\/s41598-017-05917-6","article-title":"Plasmodium berghei P47 is essential for ookinete protection from the Anopheles gambiae complement-like response.","volume":"7","author":"Ukegbu","year":"2017","journal-title":"Sci. Rep."},{"key":"B47","first-page":"1","article-title":"Reversing the incidence of Malaria 2000\u20132015.","year":"2015","journal-title":"WHO Glob. Malar. Program"},{"key":"B48","doi-asserted-by":"publisher","first-page":"153","DOI":"10.1016\/S0092-8674(01)00199-4","article-title":"A central role for P48\/45 in malaria parasite male gamete fertility.","volume":"104","author":"Van Dijk","year":"2001","journal-title":"Cell"},{"key":"B49","doi-asserted-by":"publisher","DOI":"10.1371\/journal.ppat.1000853","article-title":"Three members of the 6-cys protein family of Plasmodium play a role in gamete fertility.","volume":"6","author":"van Dijk","year":"2010","journal-title":"PLoS Pathog"},{"key":"B50","doi-asserted-by":"publisher","first-page":"133","DOI":"10.1080\/14760584.2019.1561289","article-title":"Malaria vaccines in the eradication era: current status and future perspectives.","volume":"18","author":"Wilson","year":"2019","journal-title":"Expert. Rev. Vaccines"},{"key":"B51","year":"2019","journal-title":"World Malaria Report 2019."},{"key":"B52","doi-asserted-by":"publisher","first-page":"759","DOI":"10.1021\/acsbiomaterials.9b01255","article-title":"Integrating biomaterials and immunology to improve vaccines against infectious diseases.","volume":"6","author":"Yenkoidiok-Douti","year":"2020","journal-title":"ACS Biomater. Sci. Eng."},{"key":"B53","doi-asserted-by":"publisher","DOI":"10.1038\/s41598-019-53208-z","article-title":"Engineering a virus-like particle as an antigenic platform for a Pfs47-targeted malaria transmission-blocking vaccine.","volume":"9","author":"Yenkoidiok-Douti","year":"2019","journal-title":"Sci. Rep."},{"key":"B54","doi-asserted-by":"publisher","DOI":"10.3389\/fimmu.2019.00730","article-title":"Adeno-associated virus as an effective malaria booster vaccine following adenovirus priming.","volume":"10","author":"Yusuf","year":"2019","journal-title":"Front. Immunol."},{"key":"B55","doi-asserted-by":"publisher","first-page":"E690","DOI":"10.1073\/pnas.1115485109","article-title":"Peptide tag forming a rapid covalent bond to a protein, through engineering a bacterial adhesin.","volume":"109","author":"Zakeri","year":"2012","journal-title":"Proc. Natl. Acad. Sci. U.S.A."}],"container-title":["Frontiers in Microbiology"],"original-title":[],"link":[{"URL":"https:\/\/www.frontiersin.org\/article\/10.3389\/fmicb.2020.01496\/full","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2020,7,3]],"date-time":"2020-07-03T08:15:28Z","timestamp":1593764128000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.frontiersin.org\/article\/10.3389\/fmicb.2020.01496\/full"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2020,7,3]]},"references-count":55,"alternative-id":["10.3389\/fmicb.2020.01496"],"URL":"https:\/\/doi.org\/10.3389\/fmicb.2020.01496","relation":{},"ISSN":["1664-302X"],"issn-type":[{"value":"1664-302X","type":"electronic"}],"subject":[],"published":{"date-parts":[[2020,7,3]]},"article-number":"1496"}}