{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,14]],"date-time":"2026-10-14T23:22:24Z","timestamp":1792020144784,"version":"4.4.0"},"reference-count":43,"publisher":"Frontiers Media SA","license":[{"start":{"date-parts":[[2020,7,21]],"date-time":"2020-07-21T00:00:00Z","timestamp":1595289600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["frontiersin.org"],"crossmark-restriction":true},"short-container-title":["Front. Microbiol."],"DOI":"10.3389\/fmicb.2020.01696","type":"journal-article","created":{"date-parts":[[2020,7,21]],"date-time":"2020-07-21T03:14:54Z","timestamp":1595301294000},"update-policy":"https:\/\/doi.org\/10.3389\/crossmark-policy","source":"Crossref","is-referenced-by-count":145,"title":["Clinical Validation of Two Recombinase-Based Isothermal Amplification Assays (RPA\/RAA) for the Rapid Detection of African Swine Fever Virus"],"prefix":"10.3389","volume":"11","author":[{"given":"Xiaoxu","family":"Fan","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lin","family":"Li","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yonggang","family":"Zhao","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yutian","family":"Liu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chunju","family":"Liu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Qinghua","family":"Wang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yaqin","family":"Dong","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shujuan","family":"Wang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tianying","family":"Chi","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fangfang","family":"Song","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chengyou","family":"Sun","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yingli","family":"Wang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dengchuriya","family":"Ha","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yang","family":"Zhao","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jingyue","family":"Bao","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiaodong","family":"Wu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhiliang","family":"Wang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1965","published-online":{"date-parts":[[2020,7,21]]},"reference":[{"key":"B1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0129682","article-title":"Recombinase polymerase amplification assay for rapid diagnostics of dengue infection.","volume":"10","author":"Abd El Wahed","year":"2015","journal-title":"PLoS One"},{"key":"B2","doi-asserted-by":"publisher","first-page":"693","DOI":"10.1007\/s00705-002-0946-8","article-title":"Genotyping field strains of African swine fever virus by partial p72 gene characterisation.","volume":"148","author":"Bastos","year":"2003","journal-title":"Arch. Virol."},{"key":"B3","doi-asserted-by":"publisher","first-page":"35","DOI":"10.1016\/j.talanta.2018.02.095","article-title":"Chimeric DNA\/LNA-based biosensor for the rapid detection of African swine fever virus.","volume":"184","author":"Biagetti","year":"2018","journal-title":"Talanta"},{"key":"B4","doi-asserted-by":"publisher","DOI":"10.1186\/1471-2180-13-83","article-title":"Rapid plasmid replicon typing by real time PCR melting curve analysis.","volume":"13","author":"Boot","year":"2013","journal-title":"BMC Microbiol."},{"key":"B5","doi-asserted-by":"publisher","first-page":"341","DOI":"10.4269\/ajtmh.16-0601","article-title":"Recombinase polymerase amplification compared to real-time polymerase chain reaction test for the detection of Fasciola hepatica in Human Stool.","volume":"96","author":"Cabada","year":"2017","journal-title":"Am. J. Trop. Med. Hyg."},{"key":"B6","doi-asserted-by":"publisher","first-page":"90","DOI":"10.1016\/j.diagmicrobio.2017.10.005","article-title":"Use of a rapid reverse-transcription recombinase aided amplification assay for respiratory syncytial virus detection.","volume":"90","author":"Chen","year":"2018","journal-title":"Diagn. Microbiol. Infect. Dis."},{"key":"B7","doi-asserted-by":"publisher","first-page":"2309","DOI":"10.1039\/c6lc00329j","article-title":"A centrifugal direct recombinase polymerase amplification (direct-RPA) microdevice for multiplex and real-time identification of food poisoning bacteria.","volume":"16","author":"Choi","year":"2016","journal-title":"Lab Chip"},{"key":"B8","doi-asserted-by":"publisher","first-page":"67","DOI":"10.1016\/j.jviromet.2011.01.009","article-title":"Real-time target-specific detection of loop-mediated isothermal amplification for white spot syndrome virus using fluorescence energy transfer-based probes.","volume":"173","author":"Chou","year":"2011","journal-title":"J. Virol. Methods"},{"key":"B9","doi-asserted-by":"publisher","DOI":"10.3390\/plants8050139","article-title":"Application of loop-mediated isothermal amplification in an early warning system for epidemics of an externally sourced plant virus.","volume":"8","author":"Congdon","year":"2019","journal-title":"Plants"},{"key":"B10","doi-asserted-by":"publisher","first-page":"5615","DOI":"10.1021\/ac5011298","article-title":"Quantification of HIV-1 DNA using real-time recombinase polymerase amplification.","volume":"86","author":"Crannell","year":"2014","journal-title":"Anal. Chem."},{"key":"B11","doi-asserted-by":"publisher","first-page":"168","DOI":"10.1016\/j.virusres.2012.09.013","article-title":"Laboratory methods to study African swine fever virus.","volume":"173","author":"de Leon","year":"2013","journal-title":"Virus Res."},{"key":"B12","doi-asserted-by":"crossref","first-page":"34","DOI":"10.1016\/j.antiviral.2019.02.018","article-title":"African swine fever.","volume":"165","author":"Dixon","year":"2019","journal-title":"Antiviral Res."},{"key":"B13","doi-asserted-by":"publisher","first-page":"145","DOI":"10.1016\/0021-9975(89)90125-4","article-title":"Infection of pigs with the Cameroon isolate (Cam\/82) of African swine fever virus.","volume":"100","author":"Ekue","year":"1989","journal-title":"J. Comp. Pathol."},{"key":"B14","doi-asserted-by":"crossref","DOI":"10.2807\/1560-7917.ES.2015.20.44.30053","article-title":"Development and deployment of a rapid recombinase polymerase amplification Ebola virus detection assay in Guinea in 2015.","volume":"20","author":"Faye","year":"2015","journal-title":"Euro Surveill."},{"key":"B15","doi-asserted-by":"publisher","DOI":"10.3390\/v9050103","article-title":"African swine fever virus: a review.","volume":"9","author":"Galindo","year":"2017","journal-title":"Viruses"},{"key":"B16","doi-asserted-by":"publisher","first-page":"304","DOI":"10.1016\/j.snb.2018.07.164","article-title":"Cross-priming amplification combined with immunochromatographic strip for rapid on-site detection of African swine fever virus.","volume":"274","author":"Gao","year":"2018","journal-title":"Sens. Actuators B Chem."},{"key":"B17","doi-asserted-by":"crossref","first-page":"2131","DOI":"10.3201\/eid2411.181274","article-title":"Molecular characterization of African swine fever virus, China, 2018.","volume":"24","author":"Ge","year":"2018","journal-title":"Emerg. Infect. Dis."},{"key":"B18","doi-asserted-by":"publisher","first-page":"31","DOI":"10.1016\/j.mimet.2018.12.017","article-title":"Real-time recombinase polymerase amplification assay for the rapid and sensitive detection of Campylobacter jejuni in food samples.","volume":"157","author":"Geng","year":"2019","journal-title":"J. Microbiol. Methods"},{"key":"B19","doi-asserted-by":"publisher","first-page":"817","DOI":"10.1016\/j.jmoldx.2017.06.007","article-title":"Evaluation and clinical validation of two field-deployable reverse transcription-insulated isothermal PCR assays for the detection of the middle east respiratory syndrome-Coronavirus.","volume":"19","author":"Go","year":"2017","journal-title":"J. Mol. Diagn."},{"key":"B20","first-page":"3270","article-title":"[Establishment of a real-time fluorescent recombinase polymerase amplification (RPA) for the detection of African swine fever virus].","volume":"44","author":"Ha","year":"2017","journal-title":"China Anim. Husbandry Vet. Med."},{"key":"B21","doi-asserted-by":"publisher","first-page":"1135","DOI":"10.3855\/jidc.11549","article-title":"A quite sensitive fluorescent loop-mediated isothermal amplification for rapid detection of respiratory syncytial virus.","volume":"13","author":"Hu","year":"2019","journal-title":"J. Infect. Dev. Ctries."},{"key":"B22","doi-asserted-by":"publisher","first-page":"68","DOI":"10.1016\/j.jviromet.2009.11.034","article-title":"Detection of African swine fever virus by loop-mediated isothermal amplification.","volume":"164","author":"James","year":"2010","journal-title":"J. Virol. Methods"},{"key":"B23","doi-asserted-by":"publisher","first-page":"53","DOI":"10.1016\/s0166-0934(02)00189-1","article-title":"Development of a TaqMan PCR assay with internal amplification control for the detection of African swine fever virus.","volume":"107","author":"King","year":"2003","journal-title":"J. Virol. Methods"},{"key":"B24","doi-asserted-by":"publisher","first-page":"31","DOI":"10.1039\/c8an01621f","article-title":"Review: a comprehensive summary of a decade development of the recombinase polymerase amplification.","volume":"144","author":"Li","year":"2018","journal-title":"Analyst"},{"key":"B25","doi-asserted-by":"publisher","first-page":"14","DOI":"10.1016\/j.mcp.2019.03.007","article-title":"Direct detection of Actinobacillus pleuropneumoniae in swine lungs and tonsils by real-time recombinase polymerase amplification assay.","volume":"45","author":"Li","year":"2019","journal-title":"Mol. Cell Probes"},{"key":"B26","doi-asserted-by":"crossref","DOI":"10.1038\/s41598-018-35636-5","article-title":"Development of real-time reverse transcription recombinase polymerase amplification (RPA) for rapid detection of peste des petits ruminants virus in clinical samples and its comparison with real-time PCR test.","volume":"8","author":"Li","year":"2018","journal-title":"Sci. Rep."},{"key":"B27","doi-asserted-by":"publisher","first-page":"908","DOI":"10.1111\/tbed.13103","article-title":"Overcoming the challenges of pen-side molecular diagnosis of African swine fever to support outbreak investigations under field conditions.","volume":"66","author":"Liu","year":"2019","journal-title":"Transbound. Emerg. Dis."},{"key":"B28","doi-asserted-by":"publisher","first-page":"312","DOI":"10.3201\/eid2102.140649","article-title":"Comparative analysis of African swine fever virus genotypes and serogroups.","volume":"21","author":"Malogolovkin","year":"","journal-title":"Emerg. Infect. Dis."},{"key":"B29","doi-asserted-by":"publisher","first-page":"866","DOI":"10.1099\/jgv.0.000024","article-title":"African swine fever virus CD2v and C-type lectin gene loci mediate serological specificity.","volume":"96","author":"Malogolovkin","year":"","journal-title":"J. Gen. Virol."},{"key":"B30","doi-asserted-by":"publisher","DOI":"10.3389\/fmicb.2019.01004","article-title":"Rapid and sensitive recombinase polymerase amplification combined with lateral flow strip for detecting African swine fever virus.","volume":"10","author":"Miao","year":"2019","journal-title":"Front. Microbiol."},{"key":"B31","doi-asserted-by":"crossref","first-page":"159","DOI":"10.1016\/S0368-1742(21)80031-4","article-title":"On a form of swine fever occurring in British East Africa (Kenya Colony).","volume":"34","author":"Montgomery","year":"1921","journal-title":"J. Comp. Pathol."},{"key":"B32","doi-asserted-by":"crossref","first-page":"379","DOI":"10.1111\/tbed.12295","article-title":"African swine fever diagnosis adapted to tropical conditions by the use of dried-blood filter papers.","volume":"63","author":"Randriamparany","year":"2016","journal-title":"Transbound. Emerg. Dis."},{"key":"B33","doi-asserted-by":"crossref","first-page":"3082","DOI":"10.1039\/c2lc40423k","article-title":"A paper and plastic device for performing recombinase polymerase amplification of HIV DNA.","volume":"12","author":"Rohrman","year":"2012","journal-title":"Lab Chip"},{"key":"B34","doi-asserted-by":"crossref","first-page":"41","DOI":"10.1016\/j.tvjl.2017.12.025","article-title":"African swine fever: a re-emerging viral disease threatening the global pig industry.","volume":"233","author":"Sanchez-Cordon","year":"2018","journal-title":"Vet. J."},{"key":"B35","doi-asserted-by":"publisher","DOI":"10.1186\/s12879-019-3814-9","article-title":"A rapid and sensitive recombinase aided amplification assay to detect hepatitis B virus without DNA extraction.","volume":"19","author":"Shen","year":"2019","journal-title":"BMC Infect. Dis."},{"key":"B36","doi-asserted-by":"crossref","DOI":"10.3390\/v12010019","article-title":"Loop-mediated isothermal amplification (LAMP) for the Diagnosis of Zika Virus: a review.","volume":"12","author":"Silva","year":"2019","journal-title":"Viruses"},{"key":"B37","doi-asserted-by":"crossref","first-page":"258","DOI":"10.1039\/C4AN01683A","article-title":"Real-time microfluidic recombinase polymerase amplification for the toxin B gene of Clostridium difficile on a SlipChip platform.","volume":"140","author":"Tsaloglou","year":"2015","journal-title":"Analyst"},{"key":"B38","first-page":"308","article-title":"A recombinase polymerase amplification-based assay for rapid detection of African swine fever virus.","volume":"81","author":"Wang","year":"","journal-title":"Can. J. Vet. Res."},{"key":"B39","doi-asserted-by":"crossref","first-page":"2293","DOI":"10.1007\/s00705-017-3368-3","article-title":"Development of a real-time recombinase polymerase amplification assay for rapid and sensitive detection of porcine circovirus 2.","volume":"162","author":"Wang","year":"","journal-title":"Arch. Virol."},{"key":"B40","doi-asserted-by":"crossref","DOI":"10.1038\/srep42903","article-title":"Polymerase cross-linking spiral reaction (PCLSR) for detection of African swine fever virus (ASFV) in pigs and wild boars.","volume":"7","author":"Wozniakowski","year":"2017","journal-title":"Sci. Rep."},{"key":"B41","doi-asserted-by":"crossref","first-page":"827","DOI":"10.24425\/pjvs.2018.125597","article-title":"Comparison of loop-mediated isothermal amplification (LAMP) and cross-priming amplification (CPA) for detection of African swine fever virus.","volume":"21","author":"Wozniakowski","year":"2018","journal-title":"Pol. J. Vet. Sci."},{"key":"B42","doi-asserted-by":"crossref","first-page":"202","DOI":"10.1016\/j.mimet.2017.06.011","article-title":"Rapid detection of Salmonella with recombinase aided amplification.","volume":"139","author":"Zhang","year":"2017","journal-title":"J. Microbiol. Methods"},{"key":"B43","doi-asserted-by":"crossref","first-page":"1482","DOI":"10.1111\/tbed.12989","article-title":"Emergence of African Swine Fever in China, 2018.","volume":"65","author":"Zhou","year":"2018","journal-title":"Transbound. Emerg. Dis."}],"container-title":["Frontiers in Microbiology"],"original-title":[],"link":[{"URL":"https:\/\/www.frontiersin.org\/article\/10.3389\/fmicb.2020.01696\/full","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2024,8,10]],"date-time":"2024-08-10T08:35:02Z","timestamp":1723278902000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.frontiersin.org\/article\/10.3389\/fmicb.2020.01696\/full"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2020,7,21]]},"references-count":43,"alternative-id":["10.3389\/fmicb.2020.01696"],"URL":"https:\/\/doi.org\/10.3389\/fmicb.2020.01696","relation":{},"ISSN":["1664-302X"],"issn-type":[{"value":"1664-302X","type":"electronic"}],"subject":[],"published":{"date-parts":[[2020,7,21]]},"article-number":"1696"}}