{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,18]],"date-time":"2026-08-18T04:18:17Z","timestamp":1787026697764,"version":"build-2736575974"},"reference-count":72,"publisher":"Frontiers Media SA","license":[{"start":{"date-parts":[[2020,8,21]],"date-time":"2020-08-21T00:00:00Z","timestamp":1597968000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["frontiersin.org"],"crossmark-restriction":true},"short-container-title":["Front. Microbiol."],"DOI":"10.3389\/fmicb.2020.01988","type":"journal-article","created":{"date-parts":[[2020,8,21]],"date-time":"2020-08-21T07:55:28Z","timestamp":1597996528000},"update-policy":"https:\/\/doi.org\/10.3389\/crossmark-policy","source":"Crossref","is-referenced-by-count":28,"title":["Development of a New High-Cell Density Fermentation Strategy for Enhanced Production of a Fungus \u03b2-Glucosidase in Pichia pastoris"],"prefix":"10.3389","volume":"11","author":[{"given":"Wancang","family":"Liu","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Haibo","family":"Xiang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tao","family":"Zhang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xu","family":"Pang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jing","family":"Su","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hongyu","family":"Liu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Baiping","family":"Ma","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Liyan","family":"Yu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1965","published-online":{"date-parts":[[2020,8,21]]},"reference":[{"key":"B1","doi-asserted-by":"publisher","first-page":"5301","DOI":"10.1007\/s00253-014-5732-5","article-title":"Protein expression in Pichia pastoris: recent achievements and perspectives for heterologous protein production.","volume":"98","author":"Ahmad","year":"2014","journal-title":"Appl. Microbiol. Biotechnol."},{"key":"B2","doi-asserted-by":"publisher","first-page":"105","DOI":"10.2174\/0929866526666191014124855","article-title":"Recombinant protein production in microalgae: emerging trends.","volume":"27","author":"Ahmad","year":"2020","journal-title":"Protein Peptide Lett."},{"key":"B3","doi-asserted-by":"publisher","first-page":"1509","DOI":"10.1271\/bbb1961.47.1509","article-title":"Oxidation of methanol by the yeast, Pichia pastoris, purification and properties of the formaldehyde dehydrogenase.","volume":"47","author":"Allais","year":"1983","journal-title":"Agr. Biol. Chem."},{"key":"B4","doi-asserted-by":"publisher","first-page":"569","DOI":"10.1016\/j.bios.2014.06.009","article-title":"Enzyme orientation for direct electron transfer in an enzymatic fuel cell with alcohol oxidase and laccase electrodes.","volume":"61","author":"Arrocha","year":"2014","journal-title":"Biosens. Bioelectron."},{"key":"B5","doi-asserted-by":"publisher","first-page":"37","DOI":"10.1002\/yea.805","article-title":"Molecular characterization of the Hansenula polymorpha FLD1 gene encoding formaldehyde dehydrogenase.","volume":"19","author":"Baerends","year":"2002","journal-title":"Yeast"},{"key":"B6","doi-asserted-by":"publisher","first-page":"335","DOI":"10.1038\/nbt.1833","article-title":"Overcoming barriers to membrane protein structure determination.","volume":"29","author":"Bill","year":"2011","journal-title":"Nat. Biotechnol."},{"key":"B7","doi-asserted-by":"publisher","first-page":"9","DOI":"10.1016\/j.sbi.2015.01.005","article-title":"Pichia pastoris as an expression host for membrane protein structural biology.","volume":"32","author":"Byrne","year":"2015","journal-title":"Curr. Opin. Struc. Biol."},{"key":"B8","doi-asserted-by":"publisher","first-page":"1710","DOI":"10.1002\/bit.26947","article-title":"Deregulation of methanol metabolism reverts transcriptional limitations of recombinant Pichia pastoris (Komagataella spp) with multiple expression cassettes under control of the AOX1 promoter.","volume":"116","author":"C\u00e1mara","year":"2019","journal-title":"Biotechnol. Bioeng."},{"key":"B9","doi-asserted-by":"publisher","first-page":"1108","DOI":"10.1016\/j.biotechadv.2011.09.011","article-title":"Production of recombinant proteins by yeast cells.","volume":"30","author":"\u00c7elik","year":"2012","journal-title":"Biotechnol. Adv."},{"key":"B10","doi-asserted-by":"publisher","first-page":"2918","DOI":"10.1016\/j.biortech.2009.01.054","article-title":"Pilot treatment of wastewater from Dioscorea zingiberensis CH Wright production by anaerobic digestion combined with a biological aerated filter.","volume":"100","author":"Cheng","year":"2009","journal-title":"Bioresour. Technol."},{"key":"B11","doi-asserted-by":"publisher","first-page":"298","DOI":"10.1002\/(sici)1097-0290(20000505)68:3<298::aid-bit8>3.0.co;2-k","article-title":"Design of metabolic feed controllers: application to high\u2212density fermentations of Pichia pastoris.","volume":"68","author":"Chung","year":"2000","journal-title":"Biotechnol. Bioeng."},{"key":"B12","doi-asserted-by":"publisher","first-page":"446","DOI":"10.1074\/jbc.m116.755884","article-title":"Structures of the multidrug transporter P-glycoprotein reveal asymmetric ATP binding and the mechanism of polyspecificity.","volume":"292","author":"Esser","year":"2016","journal-title":"J. Biol. Chem."},{"key":"B13","first-page":"11","article-title":"Metabolomics study in methylotrophic yeast: a minireview.","volume":"11","author":"Fam","year":"2017","journal-title":"J. Life Sci."},{"key":"B14","doi-asserted-by":"publisher","first-page":"1541","DOI":"10.1007\/s10295-014-1491-7","article-title":"Enhanced production of Thermomyces lanuginosus lipase in Pichia pastoris via genetic and fermentation strategies.","volume":"41","author":"Fang","year":"2014","journal-title":"J. Ind. Microbiol. Biotechnol."},{"key":"B15","doi-asserted-by":"publisher","first-page":"1329","DOI":"10.1007\/s00253-007-1117-3","article-title":"Purification, characterization, and substrate specificity of a glucoamylase with steroidal saponin-rhamnosidase activity from Curvularia lunata.","volume":"76","author":"Feng","year":"","journal-title":"Appl. Microbiol. Biotechnol."},{"key":"B16","doi-asserted-by":"publisher","first-page":"6796","DOI":"10.1016\/j.tet.2007.04.076","article-title":"The substrate specificity of a glucoamylase with steroidal saponin-rhamnosidase activity from Curvularia lunata.","volume":"63","author":"Feng","year":"","journal-title":"Tetrahedron"},{"key":"B17","doi-asserted-by":"publisher","first-page":"1125","DOI":"10.1007\/s00449-012-0697-1","article-title":"Methanol\/sorbitol co-feeding induction enhanced porcine interferon-\u03b1 production by P. pastoris associated with energy metabolism shift.","volume":"35","author":"Gao","year":"2012","journal-title":"Bioproc. Biosyst. Eng."},{"key":"B18","doi-asserted-by":"publisher","first-page":"493","DOI":"10.1007\/s12010-015-1590-6","article-title":"Improving performance and operational stability of porcine interferon-\u03b1 production by Pichia pastoris with combinational induction strategy of low temperature and methanol\/sorbitol co-feeding.","volume":"176","author":"Gao","year":"2015","journal-title":"Appl. Biochem. Biotechnol."},{"key":"B19","doi-asserted-by":"publisher","first-page":"191","DOI":"10.2217\/fmb.12.133","article-title":"Pichia pastoris: protein production host and model organism for biomedical research.","volume":"8","author":"Gasser","year":"2013","journal-title":"Future Microbiol."},{"key":"B20","doi-asserted-by":"publisher","first-page":"664","DOI":"10.1002\/jctb.4619","article-title":"Methanol feeding strategy design enhances recombinant human growth hormone production by Pichia pastoris.","volume":"91","author":"G\u00fcne\u015f","year":"2016","journal-title":"J. Chem. Technol. Biotechnol."},{"key":"B21","doi-asserted-by":"publisher","first-page":"61","DOI":"10.1006\/prep.1997.0765","article-title":"High-level intracellular expression of hydroxynitrile lyase from the tropical rubber tree Hevea brasiliensis in microbial hosts.","volume":"11","author":"Hasslacher","year":"1997","journal-title":"Protein Expr. Purif."},{"key":"B22","doi-asserted-by":"publisher","first-page":"658","DOI":"10.1007\/s13238-014-0086-4","article-title":"The recombinant expression systems for structure determination of eukaryotic membrane proteins.","volume":"5","author":"He","year":"2014","journal-title":"Protein Cell"},{"key":"B23","doi-asserted-by":"publisher","first-page":"159","DOI":"10.1016\/j.procbio.2017.05.011","article-title":"Transcriptional analysis for carbon metabolism and kinetic modeling for heterologous proteins productions by Pichia pastoris in induction process with methanol\/sorbitol co-feeding.","volume":"59","author":"Jia","year":"2017","journal-title":"Process Biochem."},{"key":"B24","doi-asserted-by":"publisher","first-page":"91","DOI":"10.1016\/j.bej.2010.07.009","article-title":"Enhanced porcine interferon-\u03b1 production by recombinant Pichia pastoris with a combinational control strategy of low induction temperature and high dissolved oxygen concentration.","volume":"52","author":"Jin","year":"2010","journal-title":"Biochem. Eng. J."},{"key":"B25","doi-asserted-by":"publisher","first-page":"236","DOI":"10.1016\/j.jbiotec.2007.04.004","article-title":"Regulation of alcohol oxidase of a recombinant Pichia pastoris Mut+ strain in transient continuous cultures.","volume":"130","author":"Jungo","year":"2007","journal-title":"J. Biotechnol."},{"key":"B26","doi-asserted-by":"publisher","first-page":"5867","DOI":"10.1002\/jcp.29583","article-title":"Pichia pastoris: a highly successful expression system for optimal synthesis of heterologous proteins.","volume":"235","author":"Karbalaei","year":"2020","journal-title":"J. Cell. Physiol."},{"key":"B27","doi-asserted-by":"crossref","DOI":"10.1038\/s41598-017-11819-4","article-title":"Improvement of a fermentation process for the production of two Pf AMA1-DiCo-based malaria vaccine candidates in Pichia pastoris.","volume":"7","author":"Kastilan","year":"2017","journal-title":"Sci. Rep"},{"key":"B28","doi-asserted-by":"publisher","first-page":"455","DOI":"10.1016\/0076-6879(90)88072-i","article-title":"Formaldehyde dehydrogenase from methylotrophic yeasts.","volume":"188","author":"Kato","year":"1990","journal-title":"Method Enzymol."},{"key":"B29","doi-asserted-by":"publisher","first-page":"565","DOI":"10.1007\/s00449-016-1538-4","article-title":"Enhanced truncated-t-PA (CT-b) expression in high-cell-density fed-batch cultures of Pichia pastoris through optimization of a mixed feeding strategy by response surface methodology.","volume":"39","author":"Kazemali","year":"2016","journal-title":"Bioproc. Biosyst. Eng."},{"key":"B30","doi-asserted-by":"publisher","first-page":"25","DOI":"10.1007\/978-4-431-56030-2_2","article-title":"Expression of proteins in insect and mammalian cells.","volume":"147","author":"Kita","year":"2016","journal-title":"Adv. Method Struct. Biol."},{"key":"B31","doi-asserted-by":"publisher","first-page":"288","DOI":"10.1016\/j.procbio.2020.02.025","article-title":"Influence of phosphate anions on the stability of immobilized enzymes. Effect of enzyme nature, immobilization protocol and inactivation conditions.","volume":"95","author":"Kornecki","year":"2020","journal-title":"Process Biochem."},{"key":"B32","doi-asserted-by":"publisher","DOI":"10.1038\/227680a0","article-title":"Cleavage of structural proteins during the assembly of the head of bacteriophage T4.","volume":"227","author":"Laemmli","year":"1970","journal-title":"Nature"},{"key":"B33","doi-asserted-by":"publisher","first-page":"1147","DOI":"10.1016\/s0032-9592(02)00280-7","article-title":"High dissolved oxygen tension enhances heterologous protein expression by recombinant Pichia pastoris.","volume":"38","author":"Lee","year":"2003","journal-title":"Process Biochem."},{"key":"B34","doi-asserted-by":"publisher","first-page":"561","DOI":"10.1038\/nature17666","article-title":"Crystal structure of the human sterol transporter ABCG5\/ABCG8.","volume":"533","author":"Lee","year":"2016","journal-title":"Nature"},{"key":"B35","doi-asserted-by":"publisher","first-page":"77","DOI":"10.1016\/j.pep.2018.07.013","article-title":"High-level expression and purification of active scorpion long-chain neurotoxin Bj\u03b1IT from Pichia pastoris.","volume":"152","author":"Li","year":"2018","journal-title":"Protein Expr. Purif."},{"key":"B36","article-title":"Scaling-up fermentation of Pichia pastoris to demonstration-scale using new methanol-feeding strategy and increased air pressure instead of pure oxygen supplement.","volume":"6","author":"Liu","year":"2016","journal-title":"Sci. Rep."},{"key":"B37","doi-asserted-by":"publisher","first-page":"258","DOI":"10.1080\/07388551.2018.1554620","article-title":"Fed-batch high-cell-density fermentation strategies for Pichia pastoris growth and production.","volume":"39","author":"Liu","year":"","journal-title":"Crit. Rev. Biotechnol."},{"key":"B38","doi-asserted-by":"publisher","first-page":"1226","DOI":"10.1111\/1751-7915.13420","article-title":"Expression of multidrug transporter P-glycoprotein in Pichia pastoris affects the host\u2019s methanol metabolism.","volume":"12","author":"Liu","year":"","journal-title":"Microb. Biotechnol"},{"key":"B39","doi-asserted-by":"publisher","first-page":"867","DOI":"10.1007\/s10295-015-1617-6","article-title":"Pilot studies on scale-up biocatalysis of 7-\u03b2-xylosyl-10-deacetyltaxol and its analogues by an engineered yeast.","volume":"42","author":"Liu","year":"2015","journal-title":"J. Ind. Microbiol. Biotechnol."},{"key":"B40","doi-asserted-by":"publisher","first-page":"1177","DOI":"10.1016\/j.biotechadv.2015.05.008","article-title":"Cultivation strategies to enhance productivity of Pichia pastoris: a review.","volume":"33","author":"Looser","year":"2015","journal-title":"Biotechnol. Adv."},{"key":"B41","doi-asserted-by":"publisher","first-page":"76","DOI":"10.1007\/s12257-011-0201-9","article-title":"A new methanol-feeding strategy for the improved production of \u03b2-galactosidase in high cell-density fed-batch cultures of Pichia pastoris Mut+ strains.","volume":"17","author":"Maghsoudi","year":"2012","journal-title":"Biotechnol. Biopro. Eng."},{"key":"B42","doi-asserted-by":"publisher","first-page":"687","DOI":"10.1002\/9783527807796.ch19","article-title":"Industrial microorganisms: Pichia pastoris.","volume":"1","author":"Mattanovich","year":"2017","journal-title":"Ind. Biotechnol."},{"key":"B43","doi-asserted-by":"publisher","first-page":"229","DOI":"10.1002\/pca.2887","article-title":"New insights into diosgenin biosynthesis pathway and its regulation in Trigonella foenum-graecum L.","volume":"31","author":"Mohammadi","year":"2019","journal-title":"Phytochem. Anal."},{"key":"B44","doi-asserted-by":"publisher","first-page":"315","DOI":"10.1111\/1751-7915.13498","article-title":"Rationale-based selection of optimal operating strategies and gene dosage impact on recombinant protein production in Komagataella phaffii (Pichia pastoris).","volume":"13","author":"Nieto-Taype","year":"2020","journal-title":"Microb. Biotechnol."},{"key":"B45","first-page":"93","article-title":"Heterologous expression of Trichoderma atroviride endochitinase Ech42 in Pichia pastoris at low and high dissolved oxygen tensions.","volume":"13","author":"P\u00e9rez-Mart\u00ednez","year":"2014","journal-title":"Revist. Mexicana Ingenier\u00eda Qu\u00edmica"},{"key":"B46","doi-asserted-by":"publisher","first-page":"307","DOI":"10.1002\/bit.21112","article-title":"Scale up of a viscous fungal fermentation: application of scale-up criteria with regime analysis and operating boundary conditions.","volume":"96","author":"Pollard","year":"2007","journal-title":"Biotechnol. Bioeng."},{"key":"B47","doi-asserted-by":"publisher","first-page":"91","DOI":"10.1016\/j.bej.2010.07.017","article-title":"Bioprocess engineering aspects of heterologous protein production in Pichia pastoris: a review.","volume":"64","author":"Potvin","year":"2012","journal-title":"Biochem. Eng. J."},{"key":"B48","doi-asserted-by":"publisher","first-page":"103","DOI":"10.1016\/j.jbiotec.2003.10.029","article-title":"Expression of a Rhizopus oryzae lipase in Pichia pastoris under control of the nitrogen source-regulated formaldehyde dehydrogenase promoter.","volume":"109","author":"Resina","year":"2004","journal-title":"J. Biotechnol."},{"key":"B49","doi-asserted-by":"publisher","first-page":"1412","DOI":"10.1002\/pro.3668","article-title":"New tools for recombinant protein production in Escherichia coli: a 5-year update.","volume":"28","author":"Rosano","year":"2019","journal-title":"Protein Sci."},{"key":"B50","doi-asserted-by":"publisher","first-page":"157","DOI":"10.1134\/s0026893319020146","article-title":"Strategies for optimizing recombinant protein synthesis in plant cells: classical approaches and new directions.","volume":"53","author":"Rozov","year":"2019","journal-title":"Mol. Biol."},{"key":"B51","doi-asserted-by":"crossref","DOI":"10.1186\/s12934-016-0565-9","article-title":"Functional inclusion bodies produced in the yeast Pichia pastoris.","volume":"15","author":"Rueda","year":"2016","journal-title":"Microb. Cell Fact."},{"key":"B52","doi-asserted-by":"publisher","first-page":"14","DOI":"10.1016\/j.bej.2015.02.027","article-title":"Cloning and expression of l-asparaginase from E. coli in eukaryotic expression system.","volume":"102","author":"Sajitha","year":"2015","journal-title":"Biochem. Eng. J."},{"key":"B53","doi-asserted-by":"publisher","first-page":"344","DOI":"10.1016\/j.jbiotec.2006.09.015","article-title":"A simple method to monitor and control methanol feeding of Pichia pastoris fermentations using mid-IR spectroscopy.","volume":"128","author":"Schenk","year":"2007","journal-title":"J. Biotechnol."},{"key":"B54","doi-asserted-by":"publisher","first-page":"870","DOI":"10.1111\/j.1567-1364.2008.00410.x","article-title":"Posttransformational vector amplification in the yeast Pichia pastoris.","volume":"8","author":"Sunga","year":"2008","journal-title":"FEMS Yeast Res."},{"key":"B55","doi-asserted-by":"crossref","first-page":"1795","DOI":"10.1023\/A:1026228518418","article-title":"On-line monitoring of the methanol concentration in Pichia pastoris cultures producing an heterologous lipase by sequential injection analysis.","volume":"25","author":"Surribas","year":"2003","journal-title":"Biotechnol. Lett."},{"key":"B56","doi-asserted-by":"publisher","first-page":"1297","DOI":"10.1271\/bbb1961.54.1297","article-title":"Screening and identification of Candida methanosorbosa as alcohol oxidase-producing methanol using yeast.","volume":"54","author":"Suye","year":"1990","journal-title":"Agric. Biol. Chem."},{"key":"B57","doi-asserted-by":"publisher","first-page":"285","DOI":"10.1002\/yea.3388","article-title":"Expression of recombinant enhanced green fluorescent protein provides insight into foreign gene-expression differences between Mut+ and MutS strains of Pichia pastoris.","volume":"36","author":"Theron","year":"2019","journal-title":"Yeast"},{"key":"B58","doi-asserted-by":"publisher","first-page":"221","DOI":"10.1016\/j.pnpbp.2014.09.011","article-title":"Acute and chronic ethanol exposure differentially alters alcohol dehydrogenase and aldehyde dehydrogenase activity in the zebrafish liver.","volume":"56","author":"Tran","year":"2015","journal-title":"Prog. Neuro Psychopharmacol. Biol. Psychiatry"},{"key":"B59","doi-asserted-by":"publisher","first-page":"438","DOI":"10.1002\/bit.10587","article-title":"Effect of methanol feeding strategies on production and yield of recombinant mouse endostatin from Pichia pastoris.","volume":"82","author":"Trinh","year":"2003","journal-title":"Biotechnol. Bioeng."},{"key":"B60","article-title":"Heterologous expression of abaecin peptide from Apis mellifera in Pichia pastoris.","volume":"16","author":"Ueira-Vieira","year":"2017","journal-title":"Microb. Cell Fact."},{"key":"B61","doi-asserted-by":"publisher","DOI":"10.1186\/1475-2859-11-103","article-title":"Physiological response of Pichia pastoris GS115 to methanol-induced high level production of the Hepatitis B surface antigen: catabolic adaptation, stress responses, and autophagic processes.","volume":"11","author":"Vanz","year":"2012","journal-title":"Microb. Cell Fact."},{"key":"B62","doi-asserted-by":"publisher","DOI":"10.1016\/j.enzmictec.2019.109465","article-title":"Rational molecular design for improving digestive enzyme resistance of beta-glucosidase from Trichoderma viride based on inhibition of bound state formation.","volume":"133","author":"Wang","year":"2020","journal-title":"Enzyme Microb. Technol."},{"key":"B63","doi-asserted-by":"publisher","first-page":"265","DOI":"10.1016\/j.jclepro.2014.05.041","article-title":"Conversion of steroid saponins into diosgenin by catalytic hydrolysis using acid-functionalized ionic liquid under microwave irradiation.","volume":"79","author":"Wang","year":"2014","journal-title":"J. Clean. Prod."},{"key":"B64","doi-asserted-by":"publisher","first-page":"581","DOI":"10.1080\/10286020.2017.1311872","article-title":"Enhancement of recombinant BmK AngM1 production in Pichia pastoris by regulating gene dosage, co-expressing with chaperones and fermenting in fed-batch mode.","volume":"19","author":"Wang","year":"2017","journal-title":"J. Asian Nat. Prod. Res."},{"key":"B65","doi-asserted-by":"publisher","first-page":"22","DOI":"10.1016\/j.enzmictec.2011.09.003","article-title":"Intracellular expression of Vitreoscilla hemoglobin improves production of Yarrowia lipolytica lipase LIP2 in a recombinant Pichia pastoris.","volume":"50","author":"Wang","year":"2012","journal-title":"Enzyme Microb. Technol."},{"key":"B66","doi-asserted-by":"publisher","first-page":"1087","DOI":"10.1002\/(sici)1097-0061(199908)15:11<1087::aid-yea436>3.0.co;2-f","article-title":"High\u2212yield secretion of recombinant gelatins by Pichia pastoris.","volume":"15","author":"Werten","year":"1999","journal-title":"Yeast"},{"key":"B67","doi-asserted-by":"publisher","first-page":"5519","DOI":"10.1007\/s00253-018-9030-5","article-title":"Isolation of endophytic fungi from Dioscorea zingiberensis CH Wright and application for diosgenin production by solid-state fermentation.","volume":"102","author":"Xiang","year":"2018","journal-title":"Appl. Microbiol. Biotechnol."},{"key":"B68","doi-asserted-by":"publisher","first-page":"182","DOI":"10.1016\/j.biotechadv.2017.11.002","article-title":"Engineering strategies for enhanced production of protein and bio-products in Pichia pastoris: a review.","volume":"36","author":"Yang","year":"2017","journal-title":"Biotechnol. Adv."},{"key":"B69","doi-asserted-by":"publisher","first-page":"1462","DOI":"10.1016\/j.procbio.2013.07.004","article-title":"Impact of NH4+ nitrogen source on the production of Rhizopus oryzae lipase in Pichia pastoris.","volume":"48","author":"Yu","year":"2013","journal-title":"Process Biochem."},{"key":"B70","doi-asserted-by":"publisher","first-page":"1","DOI":"10.1002\/1097-0290(20001005)70:1<1::aid-bit1>3.0.co;2-y","article-title":"Modelling Pichia pastoris growth on methanol and optimizing the production of a recombinant protein, the heavy-chain fragment C of botulinum, serotype A.","volume":"70","author":"Zhang","year":"2000","journal-title":"Biotechnol. Bioeng."},{"key":"B71","doi-asserted-by":"publisher","first-page":"1427","DOI":"10.1016\/j.procbio.2008.07.019","article-title":"Yellow ginger processing wastewater treatment by a hybrid biological process.","volume":"43","author":"Zhao","year":"2008","journal-title":"Process Biochem."},{"key":"B72","doi-asserted-by":"publisher","DOI":"10.1002\/biot.201800694","article-title":"Pichia pastoris as a versatile cell factory for the production of industrial enzymes and chemicals: current status and future perspectives.","volume":"14","author":"Zhu","year":"2019","journal-title":"Biotechnol. J."}],"container-title":["Frontiers in Microbiology"],"original-title":[],"link":[{"URL":"https:\/\/www.frontiersin.org\/article\/10.3389\/fmicb.2020.01988\/full","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2020,8,21]],"date-time":"2020-08-21T07:55:34Z","timestamp":1597996534000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.frontiersin.org\/article\/10.3389\/fmicb.2020.01988\/full"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2020,8,21]]},"references-count":72,"alternative-id":["10.3389\/fmicb.2020.01988"],"URL":"https:\/\/doi.org\/10.3389\/fmicb.2020.01988","relation":{},"ISSN":["1664-302X"],"issn-type":[{"value":"1664-302X","type":"electronic"}],"subject":[],"published":{"date-parts":[[2020,8,21]]},"article-number":"1988"}}