{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,13]],"date-time":"2026-10-13T18:55:39Z","timestamp":1791917739479,"version":"4.3.4"},"reference-count":58,"publisher":"Frontiers Media SA","license":[{"start":{"date-parts":[[2020,10,19]],"date-time":"2020-10-19T00:00:00Z","timestamp":1603065600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["frontiersin.org"],"crossmark-restriction":true},"short-container-title":["Front. Microbiol."],"DOI":"10.3389\/fmicb.2020.556795","type":"journal-article","created":{"date-parts":[[2020,10,19]],"date-time":"2020-10-19T04:28:15Z","timestamp":1603081695000},"update-policy":"https:\/\/doi.org\/10.3389\/crossmark-policy","source":"Crossref","is-referenced-by-count":14,"title":["Outer Membrane Vesicles From Brucella melitensis Modulate Immune Response and Induce Cytoskeleton Rearrangement in Peripheral Blood Mononuclear Cells"],"prefix":"10.3389","volume":"11","author":[{"given":"Eric Daniel","family":"Avila-Calder\u00f3n","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ol\u00edn","family":"Medina-Ch\u00e1vez","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Leopoldo","family":"Flores-Romo","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jos\u00e9 Manuel","family":"Hern\u00e1ndez-Hern\u00e1ndez","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Luis","family":"Donis-Maturano","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ahid\u00e9","family":"L\u00f3pez-Merino","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Beatriz","family":"Arellano-Reynoso","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ma. Guadalupe","family":"Aguilera-Arreola","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Enrico A.","family":"Ruiz","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zulema","family":"Gomez-Lunar","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sharon","family":"Witonsky","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Araceli","family":"Contreras-Rodr\u00edguez","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1965","published-online":{"date-parts":[[2020,10,19]]},"reference":[{"key":"B1","doi-asserted-by":"publisher","first-page":"775","DOI":"10.4103\/0377-4929.91499","article-title":"Lymphocyte activation test for diagnosis of seronegative brucellosis in humans.","volume":"54","author":"Almajid","year":"2011","journal-title":"Indian J. Pathol. Microbiol."},{"key":"B2","doi-asserted-by":"publisher","DOI":"10.3389\/fmicb.2017.02217","article-title":"Modulation of microtubule dynamics affects Brucella abortus intracellular survival, pathogen-containing vacuole maturation, and pro-inflammatory cytokine production in infected macrophages.","volume":"8","author":"Alves-Silva","year":"2017","journal-title":"Front. Microbiol."},{"key":"B3","doi-asserted-by":"publisher","DOI":"10.3389\/fmicb.2019.02714","article-title":"Proteomic analysis of membrane blebs of Brucella abortus 2308 and RB51 and their evaluation as an acellular vaccine.","volume":"10","author":"Araiza-Villanueva","year":"2019","journal-title":"Front. Microbiol."},{"key":"B4","doi-asserted-by":"publisher","DOI":"10.1155\/2012\/352493","article-title":"Characterization of outer membrane vesicles from Brucella melitensis and protection induced in mice.","volume":"2012","author":"Avila-Calder\u00f3n","year":"2012","journal-title":"Clin. Dev. Immunol."},{"key":"B5","doi-asserted-by":"publisher","DOI":"10.1155\/2013\/743509","article-title":"A history of the development of Brucella vaccines.","volume":"2013","author":"Avila-Calder\u00f3n","year":"2013","journal-title":"Biomed. Res. Int."},{"key":"B6","doi-asserted-by":"publisher","DOI":"10.3389\/fmicb.2018.02765","article-title":"The outer membrane vesicles of Aeromonas hydrophila ATCC\u00ae 7966TM: a proteomic analysis and effect on host cells.","volume":"9","author":"Avila-Calder\u00f3n","year":"2018","journal-title":"Front. Microbiol."},{"key":"B7","doi-asserted-by":"publisher","first-page":"797","DOI":"10.1111\/apm.12997","article-title":"Induction of specific cell-mediated immune responses and protection in BALB\/c mice by vaccination with outer membrane vesicles from a Brucella melitensis human isolate.","volume":"127","author":"Bagheri-Nejad","year":"2019","journal-title":"APMIS"},{"key":"B8","doi-asserted-by":"publisher","DOI":"10.1371\/journal.ppat.1004853","article-title":"Brucella abortus induces the premature death of human neutrophils through the action of its Lipopolysaccharide.","volume":"11","author":"Barquero-Calvo","year":"2015","journal-title":"PLoS Pathog."},{"key":"B9","doi-asserted-by":"publisher","DOI":"10.1038\/s41598-017-07288-4","article-title":"Bacterial membrane vesicles transport their DNA cargo into host cells.","volume":"7","author":"Bitto","year":"2017","journal-title":"Sci. Rep."},{"key":"B10","doi-asserted-by":"publisher","first-page":"5693","DOI":"10.1128\/IAI.72.10.5693-5703.2004","article-title":"Release of periplasmic proteins of Brucella suis upon acidic shock involves the outer membrane protein Omp25.","volume":"72","author":"Boigegrain","year":"2003","journal-title":"Infect. Immun."},{"key":"B11","doi-asserted-by":"publisher","first-page":"1612","DOI":"10.1016\/j.bbamcr.2013.12.011","article-title":"Protein selection and export via outer membrane vesicles.","volume":"1843","author":"Bonnington","year":"2014","journal-title":"Biochim. Biophys.Acta."},{"key":"B12","doi-asserted-by":"publisher","first-page":"1","DOI":"10.1016\/j.micres.2015.06.012","article-title":"Klebsiella pneumoniae O antigen loss alters the outer membrane protein composition and the selective packaging of proteins into secreted outer membrane vesicles.","volume":"180","author":"Cahill","year":"2015","journal-title":"Microbiol. Res."},{"key":"B13","doi-asserted-by":"publisher","DOI":"10.1186\/1475-2859-5-13","article-title":"Brucella spp. noncanonical LPS: structure, biosynthesis, and interaction with host immune system.","volume":"5","author":"Cardoso","year":"2006","journal-title":"Microb. Cell Fact."},{"key":"B14","doi-asserted-by":"publisher","first-page":"4298","DOI":"10.1021\/pr500411d","article-title":"Proteomic characterization of the outer membrane vesicle of Pseudomonas putida KT2440.","volume":"13","author":"Choi","year":"2014","journal-title":"J. Proteome Res."},{"key":"B15","doi-asserted-by":"publisher","DOI":"10.3389\/fimmu.2017.00708","article-title":"Brucella Omp25 upregulates miR-155, miR-21-5p, and miR-23b to inhibit interleukin-12 production via modulation of programmed death-1 signaling in human monocyte\/macrophages.","volume":"8","author":"Cui","year":"2017","journal-title":"Front. Immunol."},{"key":"B16","doi-asserted-by":"publisher","first-page":"500","DOI":"10.1111\/cmi.12526","article-title":"BigA is a novel adhesin of Brucella that mediates adhesion to epithelial cells.","volume":"18","author":"Czibener","year":"2016","journal-title":"Cell Microbiol."},{"key":"B17","doi-asserted-by":"publisher","first-page":"533","DOI":"10.1016\/j.yexcr.2004.06.024","article-title":"Bcl-2 prevents loss of mitochondria in CCCP-induced apoptosis.","volume":"299","author":"de Graaf","year":"2004","journal-title":"Exp. Cell Res."},{"key":"B18","doi-asserted-by":"publisher","first-page":"171","DOI":"10.1016\/j.micpath.2016.05.007","article-title":"Identification and functional analysis of invasion associated locus B (IalB) in Bartonella species.","volume":"98","author":"Deng","year":"2016","journal-title":"Microb. Pathog."},{"key":"B19","doi-asserted-by":"publisher","first-page":"4759","DOI":"10.1128\/IAI.01184-15","article-title":"CD8+ T cell exhaustion, suppressed gamma interferon production, and delayed memory response induced by chronic Brucella melitensis infection.","volume":"83","author":"Durward-Diioia","year":"2015","journal-title":"Infect. Immun."},{"key":"B20","doi-asserted-by":"publisher","first-page":"3285","DOI":"10.1128\/IAI.01382-05","article-title":"Membrane vesicles shed by Legionella pneumophila inhibit fusion of phagosomes with lysosomes.","volume":"74","author":"Fernandez-Moreira","year":"2006","journal-title":"Infect. Immun."},{"key":"B21","doi-asserted-by":"publisher","first-page":"7727","DOI":"10.1074\/jbc.M115.701540","article-title":"Structural studies of lipopolysaccharide-defective mutants from Brucella melitensis identify a core oligosaccharide critical in virulence.","volume":"291","author":"Fontana","year":"2016","journal-title":"J. Biol. Chem."},{"key":"B22","doi-asserted-by":"publisher","first-page":"609","DOI":"10.1128\/iai.55.3.609-615.1987","article-title":"Release of outer membrane fragments by exponentially growing Brucella melitensis cells.","volume":"55","author":"Gamazo","year":"1987","journal-title":"Infect. Immun."},{"key":"B23","doi-asserted-by":"publisher","first-page":"1419","DOI":"10.1128\/iai.57.5.1419-1426.1989","article-title":"Comparative analyses of proteins extracted by hot saline or released spontaneously into outer membrane blebs from field strains of Brucella ovis and Brucella melitensis.","volume":"57","author":"Gamazo","year":"1989","journal-title":"Infect. Immun."},{"key":"B24","doi-asserted-by":"publisher","first-page":"887","DOI":"10.1016\/j.ajpath.2012.05.029","article-title":"Brucella abortus-infected macrophages modulate T lymphocytes to promote osteoclastogenesis via IL-17.","volume":"181","author":"Giambartolomei","year":"2012","journal-title":"Am. J. Pathol."},{"key":"B25","doi-asserted-by":"publisher","first-page":"355","DOI":"10.1002\/iid3.173","article-title":"Increase of cells expressing PD-1 and PD-L1 and enhancement of IFN-\u03b3 production via PD-1\/PD-L1 blockade in bovine mycoplasmosis.","volume":"5","author":"Goto","year":"2017","journal-title":"Immun. Inflamm. Dis."},{"key":"B26","doi-asserted-by":"publisher","first-page":"81","DOI":"10.1016\/s0955-0674(99)80010-6","article-title":"Microtubules and signal transduction.","volume":"11","author":"Gundersen","year":"1999","journal-title":"Curr.Opin. Cell Biol."},{"key":"B27","doi-asserted-by":"publisher","DOI":"10.1155\/2011\/451694","article-title":"The PD-1\/PD-L1 (B7-H1) pathway in chronic infection-induced cytotoxic T lymphocyte exhaustion.","volume":"2011","author":"Hofmeyer","year":"2011","journal-title":"J. Biomed. Biotechnol."},{"key":"B28","doi-asserted-by":"publisher","first-page":"436","DOI":"10.1111\/1574-695X.12010","article-title":"Pluronic P85 enhances the efficacy of outer membrane vesicles as a subunit vaccine against Brucella melitensis challenge in mice.","volume":"66","author":"Jain-Gupta","year":"2012","journal-title":"FEMS Immunol. Med. Microbiol."},{"key":"B29","doi-asserted-by":"publisher","first-page":"1830","DOI":"10.1002\/eji.201040391","article-title":"IL-6: regulator of Treg\/Th17 balance.","volume":"40","author":"Kimura","year":"2010","journal-title":"Eur. J. Immunol."},{"key":"B30","doi-asserted-by":"publisher","first-page":"680","DOI":"10.1038\/227680a0","article-title":"Cleavage of structural proteins during the assembly of the head of bacteriophage T4.","volume":"227","author":"Laemmli","year":"1970","journal-title":"Nature"},{"key":"B31","doi-asserted-by":"publisher","DOI":"10.3389\/fmicb.2011.00118","article-title":"Genetic and functional diversity of Pseudomonas aeruginosa lipopolysaccharide.","volume":"2","author":"Lam","year":"2011","journal-title":"Front. Microbiol."},{"key":"B32","doi-asserted-by":"publisher","first-page":"1519","DOI":"10.1021\/pr060636a","article-title":"Extensive cell envelope modulation is associated with virulence in Brucella abortus.","volume":"6","author":"Lamontagne","year":"2007","journal-title":"J. Proteome Res."},{"key":"B33","doi-asserted-by":"publisher","first-page":"1594","DOI":"10.1021\/pr800978p","article-title":"Intracellular adaptation of Brucella abortus.","volume":"8","author":"Lamontagne","year":"2009","journal-title":"J. Proteome Res."},{"key":"B34","doi-asserted-by":"publisher","first-page":"60","DOI":"10.1016\/j.mib.2004.12.003","article-title":"Brucella lipopolysaccharide acts as a virulence factor.","volume":"8","author":"Lapaque","year":"2005","journal-title":"Curr.Opin.Microbiol."},{"key":"B35","doi-asserted-by":"publisher","DOI":"10.1007\/s11274-017-2405-4","article-title":"Brucella abortus phosphoglycero mutase and dihydrodipicolinate reductase induce Th1 and Th2-related immune responses.","volume":"34","author":"Li","year":"2018","journal-title":"World J. Microbiol.Biotechnol."},{"key":"B36","doi-asserted-by":"publisher","DOI":"10.1093\/femspd\/ftz050","article-title":"Orally-administered outer-membrane vesicles from Helicobacter pylori reduce H. pylori infection via Th2-biased immune responses in mice.","volume":"77","author":"Liu","year":"2019","journal-title":"Pathog. Dis."},{"key":"B37","doi-asserted-by":"publisher","DOI":"10.3389\/fimmu.2017.02013","article-title":"Brucella down regulates tumor necrosis factor-\u03b1 to promote intracellular survival via Omp25 regulation of different microRNAs in porcine and murine macrophages.","volume":"8","author":"Lou","year":"2018","journal-title":"Front. Immunol."},{"key":"B38","doi-asserted-by":"publisher","first-page":"e00012","DOI":"10.1128\/JB.00012-17","article-title":"Spheres of hope, packets of doom: the good and bad of outer membrane vesicles in interspecies and ecological dynamics.","volume":"199","author":"Lynch","year":"2017","journal-title":"J. Bacteriol."},{"key":"B39","doi-asserted-by":"publisher","first-page":"1120","DOI":"10.1128\/JB.02007-12","article-title":"Production of outer membrane vesicles and outer membrane tubes by Francisella novicida.","volume":"195","author":"McCaig","year":"2013","journal-title":"J. Bacteriol."},{"key":"B40","doi-asserted-by":"publisher","first-page":"241","DOI":"10.1111\/j.1574-6968.2009.01651.x","article-title":"The major outer membrane protein OprG of Pseudomonas aeruginosa contributes to cytotoxicity and forms an anaerobically regulated, cation-selective channel.","volume":"296","author":"McPhee","year":"2009","journal-title":"FEMS Microbiol. Lett."},{"key":"B41","doi-asserted-by":"publisher","first-page":"1306","DOI":"10.1128\/JB.01463-13","article-title":"Influence of O polysaccharides on biofilm development and outer membrane vesicle biogenesis in Pseudomonas aeruginosa PAO1.","volume":"196","author":"Murphy","year":"2014","journal-title":"J. Bacteriol."},{"key":"B42","doi-asserted-by":"publisher","first-page":"S233","DOI":"10.1186\/ar565","article-title":"The paradigm of IL-6: from basic science to medicine.","volume":"3","author":"Naka","year":"2002","journal-title":"Arthritis Res. Suppl."},{"key":"B43","doi-asserted-by":"publisher","first-page":"301","DOI":"10.1016\/j.phrp.2013.10.002","article-title":"Lon mutant of Brucella abortus induces tumor necrosis factor-alpha in murine J774.A1 macrophage.","volume":"4","author":"Park","year":"2013","journal-title":"Osong Public Health Res. Perspect"},{"key":"B44","doi-asserted-by":"publisher","first-page":"1518","DOI":"10.1111\/cmi.12658","article-title":"Bacterial membrane vesicles: biogenesis, immune regulation and pathogenesis.","volume":"18","author":"Pathirana","year":"2016","journal-title":"Cell Microbiol."},{"key":"B45","doi-asserted-by":"publisher","first-page":"719","DOI":"10.1016\/j.vaccine.2019.11.043","article-title":"A lipopolysaccharide-free outer membrane vesicle vaccine protects against Acinetobacter baumannii infection.","volume":"38","author":"Pulido","year":"2020","journal-title":"Vaccine"},{"key":"B46","doi-asserted-by":"publisher","first-page":"109","DOI":"10.1097\/00024382-200202000-00005","article-title":"The actin cytoskeleton: an essential component for enhanced TNF alpha production by adherent monocytes.","volume":"17","author":"Rosengart","year":"2002","journal-title":"Shock"},{"key":"B47","doi-asserted-by":"publisher","first-page":"171","DOI":"10.1016\/0378-1135(91)90091-s","article-title":"Biological properties of RB51; a stable rough strain of Brucella abortus.","volume":"28","author":"Schurig","year":"1991","journal-title":"Vet. Microbiol."},{"key":"B48","doi-asserted-by":"publisher","first-page":"392","DOI":"10.1016\/j.vetmic.2009.06.021","article-title":"Brucellosis: a re-emerging zoonosis.","volume":"140","author":"Seleem","year":"2010","journal-title":"Vet. Microbiol."},{"key":"B49","doi-asserted-by":"publisher","first-page":"90","DOI":"10.1111\/j.1365-2567.2007.02708.x","article-title":"Blockade of endogenous B7-H1 suppresses antibacterial protection after primary Listeria monocytogenes infection.","volume":"123","author":"Seo","year":"2008","journal-title":"Immunology"},{"key":"B50","doi-asserted-by":"publisher","DOI":"10.1186\/1471-2180-8-87","article-title":"Analysis of outer membrane vesicle associated proteins isolated from the plant pathogenic bacterium Xanthomonas campestris pv. Campestris.","volume":"8","author":"Sidhu","year":"2008","journal-title":"BMC Microbiol."},{"key":"B51","doi-asserted-by":"publisher","first-page":"1348","DOI":"10.3906\/sag-1506-59","article-title":"Interlukine-17 and TGF-\u03b2 levels in patients with acute brucellosis before and after treatment.","volume":"46","author":"Sofian","year":"2016","journal-title":"Turk. J. Med. Sci."},{"key":"B52","doi-asserted-by":"publisher","DOI":"10.1186\/s13567-014-0072-70","article-title":"Mutants in the lipopolysaccharide of Brucella ovis are attenuated and protect against B. ovis infection in mice.","volume":"45","author":"Soler-Llor\u00e9ns","year":"2014","journal-title":"Vet. Res."},{"key":"B53","doi-asserted-by":"publisher","first-page":"13","DOI":"10.1038\/s41579-018-0112-2","article-title":"Types and origins of bacterial membrane vesicles.","volume":"17","author":"Toyofuku","year":"2019","journal-title":"Nat. Rev. Microbiol."},{"key":"B54","doi-asserted-by":"publisher","first-page":"639","DOI":"10.1016\/j.micinf.2012.02.004","article-title":"Brucella abortus induces apoptosis of human T lymphocytes.","volume":"14","author":"Vel\u00e1squez","year":"2012","journal-title":"Microbes Infect."},{"key":"B55","doi-asserted-by":"publisher","first-page":"759","DOI":"10.1189\/jlb.4A0416-196R","article-title":"Brucella abortus down-regulates MHC class II by the IL-6-dependent inhibition of CIITA through the down modulation of IFN regulatory factor-1 (IRF-1).","volume":"101","author":"Vel\u00e1squez","year":"2016","journal-title":"J. Leukoc. Biol."},{"key":"B56","doi-asserted-by":"publisher","DOI":"10.1016\/j.vetmic.2019.07.019","article-title":"Immunization with outer membrane vesicles of avian pathogenic Escherichia coli O78 induces protective immunity in chickens.","volume":"236","author":"Wang","year":"2019","journal-title":"Vet. Microbiol."},{"key":"B57","doi-asserted-by":"crossref","first-page":"677","DOI":"10.2460\/ajvr.1996.57.05.677","article-title":"Protection of BALB\/c mice against homologous and heterologous species of Brucella by rough strain vaccines derived from Brucella melitensis and Brucella suisbiovar 4.","volume":"57","author":"Winter","year":"1996","journal-title":"Am. J. Vet. Res."},{"key":"B58","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0056539","article-title":"A potential new pathway for PD-L1 costimulation of the CD8-T cell response to Listeria monocytogenes infection.","volume":"8","author":"Xu","year":"2013","journal-title":"PLoS One"}],"container-title":["Frontiers in Microbiology"],"original-title":[],"link":[{"URL":"https:\/\/www.frontiersin.org\/article\/10.3389\/fmicb.2020.556795\/full","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2024,8,16]],"date-time":"2024-08-16T02:10:28Z","timestamp":1723774228000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.frontiersin.org\/article\/10.3389\/fmicb.2020.556795\/full"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2020,10,19]]},"references-count":58,"alternative-id":["10.3389\/fmicb.2020.556795"],"URL":"https:\/\/doi.org\/10.3389\/fmicb.2020.556795","relation":{},"ISSN":["1664-302X"],"issn-type":[{"value":"1664-302X","type":"electronic"}],"subject":[],"published":{"date-parts":[[2020,10,19]]},"article-number":"556795"}}