{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,3]],"date-time":"2026-07-03T00:12:04Z","timestamp":1783037524539,"version":"3.54.6"},"reference-count":73,"publisher":"Frontiers Media SA","license":[{"start":{"date-parts":[[2020,12,11]],"date-time":"2020-12-11T00:00:00Z","timestamp":1607644800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["frontiersin.org"],"crossmark-restriction":true},"short-container-title":["Front. Microbiol."],"abstract":"<jats:p><jats:italic>Listeria monocytogenes<\/jats:italic>(<jats:italic>Lm<\/jats:italic>) is a foodborne zoonotic pathogen that causes listeriosis with a mortality rate of 20\u201330%. Serovar 4b and 1\/2b isolates account for most of listeriosis outbreaks, however, no listeriosis vaccine is available for either prophylactic or therapeutic use. Here, we developed a triple-virulence-genes deletion vaccine strain, and evaluated its safety, immunogenicity, and cross-protective efficiency. The virulence of NTSN\u0394<jats:italic>actA<\/jats:italic>\/<jats:italic>plcB<\/jats:italic>\/<jats:italic>orfX<\/jats:italic>was reduced 794-folds compared with the parental strain. Additionally, it was completely eliminated in mice at day 7 post infection and no obvious pathological changes were observed in the organs of mice after prime-boost immunization for 23 days. These results proved that the safety of the<jats:italic>Lm<\/jats:italic>vaccine strain remarkably increased. More importantly, the NTSN\u0394<jats:italic>actA<\/jats:italic>\/<jats:italic>plcB<\/jats:italic>\/<jats:italic>orfX<\/jats:italic>strain stimulated higher anti-Listeriolysin O (LLO) antibodies, induced significantly higher expression of IFN-\u03b3, TNF-\u03b1, IL-17, and IL-6 than the control group, and afforded 100% protection against serovar 4b and 1\/2b challenges. Taken together, our research demonstrates that the triple-genes-deletion vaccine has high safety, can elicit strong Th1 type immune response, and affords efficient cross-protection against two serovar<jats:italic>Lm<\/jats:italic>strains. It is a promising vaccine for prevention of listeriosis.<\/jats:p>","DOI":"10.3389\/fmicb.2020.569544","type":"journal-article","created":{"date-parts":[[2020,12,11]],"date-time":"2020-12-11T06:09:45Z","timestamp":1607666985000},"update-policy":"https:\/\/doi.org\/10.3389\/crossmark-policy","source":"Crossref","is-referenced-by-count":12,"title":["A Cross-Protective Vaccine Against 4b and 1\/2b Listeria monocytogenes"],"prefix":"10.3389","volume":"11","author":[{"given":"Fanzeng","family":"Meng","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tengfei","family":"Zhu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hao","family":"Yao","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhiting","family":"Ling","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Youwei","family":"Feng","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Guo","family":"Li","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jing","family":"Li","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xinyu","family":"Sun","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jiaqi","family":"Chen","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chuang","family":"Meng","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xin\u2019an","family":"Jiao","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yuelan","family":"Yin","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1965","published-online":{"date-parts":[[2020,12,11]]},"reference":[{"key":"B1","doi-asserted-by":"publisher","DOI":"10.1038\/srep01809","article-title":"Prominent role for T cell-derived tumour necrosis factor for sustained control of Mycobacterium tuberculosis infection.","volume":"3","author":"Allie","year":"2013","journal-title":"Sci"},{"key":"B2","doi-asserted-by":"publisher","first-page":"310","DOI":"10.1016\/j.it.2017.01.006","article-title":"IL-17 signaling: the yin and the yang.","volume":"38","author":"Amatya","year":"2017","journal-title":"Trends Immunology."},{"key":"B3","doi-asserted-by":"publisher","first-page":"848","DOI":"10.1017\/S0950268816003083","article-title":"Multistate outbreak of Listeria monocytogenes infections linked to whole apples used in commercially produced, prepackaged caramel apples: United States, 2014-2015.","volume":"145","author":"Angelo","year":"2017","journal-title":"Epidemiol. 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