{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,13]],"date-time":"2026-10-13T18:55:38Z","timestamp":1791917738954,"version":"4.3.4"},"reference-count":58,"publisher":"Frontiers Media SA","license":[{"start":{"date-parts":[[2020,11,3]],"date-time":"2020-11-03T00:00:00Z","timestamp":1604361600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["31760261"],"award-info":[{"award-number":["31760261"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["31660035"],"award-info":[{"award-number":["31660035"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["frontiersin.org"],"crossmark-restriction":true},"short-container-title":["Front. Microbiol."],"abstract":"<jats:p>Colibacillosis is an economically important infectious disease in poultry, caused by avian pathogenic <jats:italic>Escherichia coli<\/jats:italic> (APEC). <jats:italic>Salmonella enterica<\/jats:italic> serovar Enteritidis (<jats:italic>S.<\/jats:italic> Enteritidis) is a major cause of food-borne diseases in human circulated through poultry-derived products, including meat and chicken eggs. Vaccine control is the mainstream approach for combating these infections, but it is difficult to create a vaccine for the broad-spectrum protection of poultry due to multiple serotypes of these pathogens. Our previous studies have shown that outer membrane vesicles (OMVs) derived from <jats:italic>S. enterica<\/jats:italic> serovar Typhimurium mutants with a remodeled outer membrane could induce cross-protection against heteroserotypic <jats:italic>Salmonella<\/jats:italic> infection. Therefore, in this study, we further evaluated the potential of broad-spectrum vaccines based on major outer membrane protein (OMP)-deficient OMVs, including \u0394<jats:italic>ompA<\/jats:italic>, \u0394<jats:italic>ompC<\/jats:italic>, and \u0394<jats:italic>ompD<\/jats:italic>, and determined the protection effectiveness of these candidate vaccines in murine and chicken infection models. The results showed that \u0394<jats:italic>ompA<\/jats:italic> led to an increase in the production of OMVs. Notably, \u0394<jats:italic>ompA<\/jats:italic>\u0394<jats:italic>ompC<\/jats:italic>\u0394<jats:italic>ompD<\/jats:italic> OMVs showed significantly better cross-protection against <jats:italic>S. enterica<\/jats:italic> serovar Choleraesuis, <jats:italic>S.<\/jats:italic> Enteritidis, APEC O78, and <jats:italic>Shigella flexneri<\/jats:italic> 2a than did other <jats:italic>omp<\/jats:italic>-deficient OMVs, with the exception of \u0394<jats:italic>ompA<\/jats:italic> OMVs. Subsequently, we verified the results in the chicken model, in which \u0394<jats:italic>ompA<\/jats:italic>\u0394<jats:italic>ompC<\/jats:italic>\u0394<jats:italic>ompD<\/jats:italic> OMVs elicited significant cross-protection against <jats:italic>S.<\/jats:italic> Enteritidis and APEC O78 infections. These findings further confirmed the feasibility of improving the immunogenicity of OMVs by remodeling the outer membrane and provide a new perspective for the development of broad-spectrum vaccines based on OMVs.<\/jats:p>","DOI":"10.3389\/fmicb.2020.588952","type":"journal-article","created":{"date-parts":[[2020,11,3]],"date-time":"2020-11-03T05:47:49Z","timestamp":1604382469000},"update-policy":"https:\/\/doi.org\/10.3389\/crossmark-policy","source":"Crossref","is-referenced-by-count":32,"title":["Immunization With Outer Membrane Vesicles Derived From Major Outer Membrane Protein-Deficient Salmonella Typhimurium Mutants for Cross Protection Against Salmonella Enteritidis and Avian Pathogenic Escherichia coli O78 Infection in Chickens"],"prefix":"10.3389","volume":"11","author":[{"given":"Yuxuan","family":"Chen","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kaiwen","family":"Jie","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Biaoxian","family":"Li","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Haiyan","family":"Yu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Huan","family":"Ruan","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jing","family":"Wu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiaotian","family":"Huang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Qiong","family":"Liu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1965","published-online":{"date-parts":[[2020,11,3]]},"reference":[{"key":"ref1","doi-asserted-by":"publisher","first-page":"7692","DOI":"10.4049\/jimmunol.179.11.7692","article-title":"Membrane vesicles are immunogenic facsimiles of Salmonella Typhimurium that potently activate dendritic cells, prime B and T cell responses, and stimulate protective immunity in vivo","volume":"179","author":"Alaniz","year":"2007","journal-title":"J. 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