{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,29]],"date-time":"2026-09-29T04:11:27Z","timestamp":1790655087171,"version":"4.1.0"},"reference-count":94,"publisher":"Frontiers Media SA","license":[{"start":{"date-parts":[[2020,12,11]],"date-time":"2020-12-11T00:00:00Z","timestamp":1607644800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["frontiersin.org"],"crossmark-restriction":true},"short-container-title":["Front. Microbiol."],"abstract":"<jats:p>An<jats:italic>Enterococcus durans<\/jats:italic>strain, designated OSY-EGY, was previously isolated from artisanal cheese. In this work, comparative genomic and phenotypic analyses were utilized to assess the safety characteristics and probiotic traits of the bacterium. The comparative genomic analysis revealed that the strain is distantly related to potentially pathogenic<jats:italic>Enterococcus<\/jats:italic>spp. The genome was devoid of genes encoding acquired antibiotic resistance or marker virulence factors associated with<jats:italic>Enterococcus<\/jats:italic>spp. Phenotypically, the bacterium is susceptible to vancomycin, ampicillin, tetracycline, chloramphenicol, and aminoglycosides and does not have any hemolytic or gelatinase activity, or cytotoxic effect on Caco-2 cells. Altogether, these findings confirm the lack of hazardous traits in<jats:italic>E. durans<\/jats:italic>OSY-EGY. Mining<jats:italic>E. durans<\/jats:italic>OSY-EGY genome, for probiotic-related sequences, revealed genes associated with acid and bile salts tolerance, adhesion, competitiveness, antioxidant activitiy, antimicrobial activity, essential amino acids production, and vitamins biosynthesis. Phenotypically,<jats:italic>E. durans<\/jats:italic>OSY-EGY was tolerant to acidic pH (3.0), and presence of 0.3% bile salts. The bacterium showed adhesion capability to Caco-2 cells, cholesterol-lowering effect, DPPH scavenging activity, and antimicrobial activity against several Gram-positive pathogenic bacteria. Based on the current work, we propose that<jats:italic>E. durans<\/jats:italic>OSY-EGY is a potentially safe strain with desirable probiotic and antimicrobial traits. Thus, the investigated strain could be a promising candidate for several industrial applications.<\/jats:p>","DOI":"10.3389\/fmicb.2020.608314","type":"journal-article","created":{"date-parts":[[2020,12,11]],"date-time":"2020-12-11T05:18:10Z","timestamp":1607663890000},"update-policy":"https:\/\/doi.org\/10.3389\/crossmark-policy","source":"Crossref","is-referenced-by-count":43,"title":["Assessment of Safety and Probiotic Traits of Enterococcus durans OSY-EGY, Isolated From Egyptian Artisanal Cheese, Using Comparative Genomics and Phenotypic Analyses"],"prefix":"10.3389","volume":"11","author":[{"given":"Walaa E.","family":"Hussein","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ahmed G.","family":"Abdelhamid","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Diana","family":"Rocha-Mendoza","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Israel","family":"Garc\u00eda-Cano","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ahmed E.","family":"Yousef","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1965","published-online":{"date-parts":[[2020,12,11]]},"reference":[{"key":"B1","doi-asserted-by":"publisher","DOI":"10.1186\/1471-2164-12-402","article-title":"BLAST ring image generator (BRIG): simple prokaryote genome comparisons.","volume":"12","author":"Alikhan","year":"2011","journal-title":"BMC Genom."},{"key":"B2","doi-asserted-by":"publisher","first-page":"844","DOI":"10.5851\/kosfa.2019.e57","article-title":"Potential evaluation and health fostering intrinsic traits of novel probiotic strain Enterococcus durans F3 isolated from the gut of fresh water fish catla catla.","volume":"39","author":"Alshammari","year":"2019","journal-title":"Food Sci. Anim. Resour."},{"key":"B3","doi-asserted-by":"publisher","first-page":"457","DOI":"10.1590\/S1516-89132013000300014","article-title":"The genus Enterococcus as probiotic: safety concerns.","volume":"56","author":"Ara\u00fajo","year":"2013","journal-title":"Braz. Arch. Biol. Technol."},{"key":"B4","doi-asserted-by":"publisher","DOI":"10.3389\/fmicb.2019.00881","article-title":"Probiotic potential and safety evaluation of Enterococcus faecalis OB14 and OB15, isolated from traditional Tunisian testouri cheese and rigouta, using physiological and genomic analysis.","volume":"10","author":"Baccouri","year":"2019","journal-title":"Front. Microbiol."},{"key":"B5","doi-asserted-by":"publisher","first-page":"801","DOI":"10.1111\/1541-4337.12212","article-title":"Biogenic amines in dairy products: origin, incidence, and control means.","volume":"15","author":"Benkerroum","year":"2016","journal-title":"Compr. Rev. Food Sci. Food Saf."},{"key":"B6","doi-asserted-by":"publisher","DOI":"10.1186\/s12866-017-0962-1","article-title":"Comparative genomics of Enterococcus spp. isolated from bovine feces.","volume":"17","author":"Beukers","year":"2017","journal-title":"BMC Microbiol."},{"key":"B7","doi-asserted-by":"publisher","first-page":"597","DOI":"10.1006\/fmic.2001.0435","article-title":"Enterococci vs non-lactic acid microflora as hygiene indicators for sweetened yoghurt.","volume":"18","author":"Birollo","year":"2001","journal-title":"Food Microbiol."},{"key":"B8","doi-asserted-by":"publisher","first-page":"402","DOI":"10.1128\/AEM.06588-11","article-title":"Salivaricin D, a novel intrinsically trypsin-resistant lantibiotic from Streptococcus salivarius 5M6c isolated from a healthy infant.","volume":"78","author":"Birri","year":"2012","journal-title":"Appl. Environ. Microbiol."},{"key":"B9","doi-asserted-by":"publisher","first-page":"W36","DOI":"10.1093\/nar\/gkx319","article-title":"antiSMASH 4.0-improvements in chemistry prediction and gene cluster boundary identification.","volume":"45","author":"Blin","year":"2018","journal-title":"Nucleic Acids Res."},{"key":"B10","doi-asserted-by":"publisher","DOI":"10.1038\/s41598-018-30729-7","article-title":"Colicin FY inhibits pathogenic Yersinia enterocolitica in mice.","volume":"8","author":"Bos\u00e1k","year":"2018","journal-title":"Sci. Rep."},{"key":"B11","doi-asserted-by":"publisher","first-page":"25","DOI":"10.1016\/S0023-6438(95)80008-5","article-title":"Use of a free radical method to evaluate antioxidant activity.","volume":"28","author":"Brand-Williams","year":"1995","journal-title":"LWT Food Sci. Technol."},{"key":"B12","doi-asserted-by":"publisher","first-page":"2038","DOI":"10.1002\/pmic.200600773","article-title":"Proteome phenotyping of acid stress-resistant mutants of Lactococcus lactis MG1363.","volume":"7","author":"Budin-Verneuil","year":"2007","journal-title":"Proteomics"},{"key":"B13","doi-asserted-by":"publisher","DOI":"10.1186\/s13099-017-0162-4","article-title":"Strain-specific probiotic properties of lactic acid bacteria and their interference with human intestinal pathogens invasion.","volume":"9","author":"Campana","year":"2017","journal-title":"Gut Pathog."},{"key":"B14","doi-asserted-by":"publisher","first-page":"289","DOI":"10.1007\/s13213-017-1258-2","article-title":"In vitro assessment of safety and probiotic potential characteristics of Lactobacillus strains isolated from water buffalo mozzarella cheese.","volume":"67","author":"Casarotti","year":"2017","journal-title":"Ann. Microbiol."},{"key":"B15","doi-asserted-by":"publisher","first-page":"D325","DOI":"10.1093\/nar\/gki008","article-title":"VFDB: a reference database for bacterial virulence factors.","volume":"33","author":"Chen","year":"2005","journal-title":"Nucleic Acids Res."},{"key":"B16","doi-asserted-by":"publisher","first-page":"659","DOI":"10.1109\/TVLSI.2012.2196060","article-title":"Reconfigurable accelerator for the word-matching stage of BLASTN.","volume":"21","author":"Chen","year":"2013","journal-title":"IEEE Trans. Very Larg. Scale Integr. Syst."},{"key":"B17","doi-asserted-by":"publisher","first-page":"210","DOI":"10.1016\/J.LWT.2015.01.019","article-title":"Cholesterol-lowering effects of a putative probiotic strain Lactobacillus plantarum EM isolated from kimchi.","volume":"62","author":"Choi","year":"2015","journal-title":"LWT Food Sci. Technol."},{"key":"B18","doi-asserted-by":"publisher","first-page":"1065","DOI":"10.1007\/s00217-007-0632-x","article-title":"Adhesion and aggregation properties of probiotic and pathogen strains.","volume":"226","author":"Collado","year":"2008","journal-title":"Eur. Food Res. Technol."},{"key":"B19","doi-asserted-by":"publisher","first-page":"438","DOI":"10.1046\/j.1365-2672.2000.00845.x","article-title":"Adhesion, autoaggregation and hydrophobicity of 13 strains of Bifidobacterium longum.","volume":"31","author":"Del Re","year":"2000","journal-title":"Lett. Appl. Microbiol."},{"key":"B20","doi-asserted-by":"publisher","first-page":"275","DOI":"10.1007\/s12263-011-0226-x","article-title":"Antibiotic resistance determinants in the interplay between food and gut microbiota.","volume":"6","author":"Devirgiliis","year":"2011","journal-title":"Genes Nutr."},{"key":"B21","doi-asserted-by":"publisher","first-page":"533","DOI":"10.1016\/J.IJMM.2007.02.008","article-title":"Comparative genomic analysis for the presence of potential Enterococcal virulence factors in the probiotic Enterococcus faecalis strain Symbioflor 1.","volume":"297","author":"Domann","year":"2007","journal-title":"Int. J. Med. Microbiol."},{"key":"B22","doi-asserted-by":"publisher","first-page":"11971","DOI":"10.1073\/pnas.1002601107","article-title":"Delivery mode shapes the acquisition and structure of the initial microbiota across multiple body habitats in newborns.","volume":"107","author":"Dominguez-Bello","year":"2010","journal-title":"Proc. Natl. Acad. Sci. U.S.A."},{"key":"B23","doi-asserted-by":"publisher","first-page":"1628","DOI":"10.1128\/AEM.67.4.1628-1635.2001","article-title":"Molecular screening of Enterococcus virulence determinants and potential for genetic exchange between food and medical isolates.","volume":"67","author":"Eaton","year":"2001","journal-title":"Appl. Environ. Microbiol."},{"key":"B24","doi-asserted-by":"publisher","DOI":"10.2903\/j.efsa.2011.2393","article-title":"EFSA panel on biological hazards (BIOHAZ). Scientific opinion on risk based control of biogenic amine formation in fermented foods.","volume":"9","year":"2011","journal-title":"EFSA J."},{"key":"B25","doi-asserted-by":"publisher","first-page":"2740","DOI":"10.2903\/j.efsa.2012.2740","article-title":"EFSA panel on additives and products or substances used in animal feed (FEEDAP). Guidance on the assessment of bacterial susceptibility to antimicrobials of human and veterinary importance.","volume":"10","year":"","journal-title":"EFSA J."},{"key":"B26","doi-asserted-by":"publisher","DOI":"10.2903\/j.efsa.2012.2682","article-title":"EFSA panel on additives and products or substances used in animal feed (FEEDAP). Guidance on the safety assessment of Enterococcus faecium in animal.","volume":"10","year":"","journal-title":"EFSA J."},{"key":"B27","article-title":"Probiotics in food: health and nutritional properties and guidelines for evaluation.","volume":"85","year":"2001","journal-title":"Food Nutr. Pap."},{"key":"B28","doi-asserted-by":"publisher","first-page":"1","DOI":"10.1016\/j.ijfoodmicro.2005.06.026","article-title":"The role and application of Enterococci in food and health.","volume":"106","author":"Foulqui\u00e9 Moreno","year":"2006","journal-title":"Int. J. Food Microbiol."},{"key":"B29","doi-asserted-by":"publisher","first-page":"1","DOI":"10.1016\/S0168-1605(99)00007-0","article-title":"Enterococci at the crossroads of food safety?","volume":"47","author":"Franz","year":"1999","journal-title":"Int. J. Food Microbiol."},{"key":"B30","doi-asserted-by":"publisher","first-page":"125","DOI":"10.1016\/J.IJFOODMICRO.2011.08.014","article-title":"Enterococci as probiotics and their implications in food safety.","volume":"151","author":"Franz","year":"2011","journal-title":"Int. J. Food Microbiol."},{"key":"B31","doi-asserted-by":"publisher","first-page":"S15","DOI":"10.1016\/j.ijfoodmicro.2010.02.031","article-title":"Probiotics and prebiotics in animal feeding for safe food production.","volume":"141","author":"Gagg\u00eca","year":"2010","journal-title":"Int. J. Food Microbiol."},{"key":"B32","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.02033-16","article-title":"Characterization of class IIa bacteriocin resistance in Enterococcus faecium.","volume":"61","author":"Geldart","year":"2017","journal-title":"Antimicrob. Agents Chemother."},{"key":"B33","doi-asserted-by":"publisher","DOI":"10.1186\/1741-7007-4-41","article-title":"Composition-based statistics and translated nucleotide searches: improving the TBLASTN module of BLAST.","volume":"4","author":"Gertz","year":"2006","journal-title":"BMC Biol."},{"key":"B34","doi-asserted-by":"publisher","DOI":"10.1186\/s12864-018-5043-9","article-title":"Comparative genome analysis reveals key genetic factors associated with probiotic property in Enterococcus faecium strains.","volume":"19","author":"Ghattargi","year":"2018","journal-title":"BMC Genom."},{"key":"B35","doi-asserted-by":"publisher","DOI":"10.1186\/1471-2180-9-165","article-title":"Persistence of colicinogenic Escherichia coli in the mouse gastrointestinal tract.","volume":"9","author":"Gillor","year":"2009","journal-title":"BMC Microbiol."},{"key":"B36","doi-asserted-by":"publisher","first-page":"163","DOI":"10.1111\/j.1574-6976.2002.tb00608.x","article-title":"Enterococci from foods.","volume":"26","author":"Giraffa","year":"2002","journal-title":"FEMS Microbiol. Rev."},{"key":"B37","doi-asserted-by":"publisher","first-page":"732","DOI":"10.4315\/0362-028X-60.6.732","article-title":"Enterococci isolated from dairy products: a review of risks and potential technological use.","volume":"60","author":"Giraffa","year":"1997","journal-title":"J. Food Prot."},{"key":"B38","doi-asserted-by":"publisher","first-page":"W52","DOI":"10.1093\/nar\/gkm360","article-title":"CRISPRFinder: a web tool to identify clustered regularly interspaced short palindromic repeats.","volume":"35","author":"Grissa","year":"2007","journal-title":"Nucleic Acids Res."},{"key":"B39","doi-asserted-by":"publisher","first-page":"358","DOI":"10.1016\/B978-0-12-384730-0.00396-7","article-title":"Indicator organisms","author":"Halkman","year":"2014","journal-title":"Encyclopedia of Food Microbiology"},{"key":"B40","doi-asserted-by":"publisher","DOI":"10.1186\/1471-2180-11-63","article-title":"Comparative proteomic analysis of Lactobacillus plantarum for the identification of key proteins in bile tolerance.","volume":"11","author":"Hamon","year":"2011","journal-title":"BMC Microbiol."},{"key":"B41","doi-asserted-by":"publisher","DOI":"10.3389\/fmicb.2018.01791","article-title":"The genus Enterococcus: between probiotic potential and safety concerns\u2014an update.","volume":"9","author":"Hanchi","year":"2018","journal-title":"Front. Microbiol."},{"key":"B42","doi-asserted-by":"publisher","first-page":"168","DOI":"10.1128\/AEM.02023-2026","article-title":"Isolation and identification of a Paenibacillus polymyxa strain that coproduces a novel lantibiotic and polymyxin.","volume":"73","author":"He","year":"2007","journal-title":"Appl. Environ. Microbiol."},{"key":"B43","doi-asserted-by":"publisher","first-page":"1963","DOI":"10.1128\/jcm.40.6.1963-1971.2002","article-title":"Multilocus sequence typing scheme for Enterococcus faecium.","volume":"40","author":"Homan","year":"2002","journal-title":"J. Clin. Microbiol."},{"key":"B44","doi-asserted-by":"crossref","DOI":"10.1128\/MRA.00303-19","article-title":"Draft genome sequence of Enterococcus durans OSY-EGY, a multiple-antimicrobial-peptide producer isolated from Egyptian hard cheese.","volume":"8","author":"Hussein","year":"2019","journal-title":"Microbiol. Resour. Announc."},{"key":"B45","doi-asserted-by":"publisher","DOI":"10.3389\/fmicb.2016.01504","article-title":"Stuck in the middle: fibronectin-binding proteins in Gram-positive bacteria.","volume":"7","author":"Hymes","year":"2016","journal-title":"Front. Microbiol."},{"key":"B46","doi-asserted-by":"publisher","first-page":"189","DOI":"10.3412\/jsb.72.189","article-title":"Pathogenicity of Enterococci.","volume":"72","author":"Ike","year":"2017","journal-title":"Nippon Saikingaku Zasshi"},{"key":"B47","doi-asserted-by":"publisher","first-page":"141","DOI":"10.1007\/s12602-014-9166-2","article-title":"Probiotic properties of lactic acid bacteria isolated from water-buffalo mozzarella cheese.","volume":"6","author":"Jeronymo-Ceneviva","year":"2014","journal-title":"Probiot. Antimicrob. Proteins"},{"key":"B48","doi-asserted-by":"publisher","first-page":"D566","DOI":"10.1093\/nar\/gkw1004","article-title":"CARD 2017: expansion and model-centric curation of the comprehensive antibiotic resistance database.","volume":"45","author":"Jia","year":"2017","journal-title":"Nucleic Acids Res."},{"key":"B49","doi-asserted-by":"publisher","first-page":"D590","DOI":"10.1093\/nar\/gky962","article-title":"New approach for understanding genome variations in KEGG.","volume":"47","author":"Kanehisa","year":"2019","journal-title":"Nucleic Acids Res."},{"key":"B50","doi-asserted-by":"publisher","first-page":"255","DOI":"10.1016\/S0168-1605(03)00188-0","article-title":"Safety aspects of Enterococci from the medical point of view.","volume":"88","author":"Kayser","year":"2003","journal-title":"Int. J. Food Microbiol."},{"key":"B51","doi-asserted-by":"publisher","first-page":"412","DOI":"10.1007\/s13197-011-0251-1","article-title":"Genesis and development of DPPH method of antioxidant assay.","volume":"48","author":"Kedare","year":"2011","journal-title":"J. Food Sci. Technol."},{"key":"B52","doi-asserted-by":"publisher","first-page":"1","DOI":"10.1016\/J.IJFOODMICRO.2010.03.005","article-title":"Enterocins in food preservation.","volume":"141","author":"Khan","year":"2010","journal-title":"Int. J. Food Microbiol."},{"key":"B53","doi-asserted-by":"publisher","first-page":"269","DOI":"10.1016\/S0168-1605(03)00190-9","article-title":"Occurrence and spread of antibiotic resistances in Enterococcus faecium.","volume":"88","author":"Klare","year":"2003","journal-title":"Int. J. Food Microbiol."},{"key":"B54","doi-asserted-by":"publisher","DOI":"10.1186\/1297-9716-43-58","article-title":"Feeding of the probiotic bacterium Enterococcus faecium NCIMB 10415 differentially affects shedding of enteric viruses in pigs.","volume":"43","author":"Kreuzer","year":"2012","journal-title":"Vet. Res."},{"key":"B55","doi-asserted-by":"publisher","DOI":"10.1128\/genomeA.00265-13","article-title":"Draft genome sequence of the tyramine producer Enterococcus durans strain IPLA 655.","volume":"1","author":"Ladero","year":"2013","journal-title":"Genome Announc."},{"key":"B56","doi-asserted-by":"publisher","first-page":"2334","DOI":"10.1128\/JB.00259-12","article-title":"Comparative analysis of the first complete Enterococcus faecium genome.","volume":"194","author":"Lam","year":"2012","journal-title":"J. Bacteriol."},{"key":"B57","doi-asserted-by":"publisher","first-page":"454","DOI":"10.1016\/j.mib.2006.07.001","article-title":"Identification of high-risk enterococcal clonal complexes: global dispersion and antibiotic resistance.","volume":"9","author":"Leavis","year":"2006","journal-title":"Curr. Opin. Microbiol."},{"key":"B58","doi-asserted-by":"publisher","DOI":"10.1186\/s13099-018-0260-y","article-title":"Complete genome sequence of Enterococcus durans KLDS6.0933, a potential probiotic strain with high cholesterol removal ability.","volume":"10","author":"Li","year":"","journal-title":"Gut Pathog."},{"key":"B59","doi-asserted-by":"publisher","DOI":"10.3389\/fmicb.2018.01943","article-title":"Evaluating the safety of potential probiotic Enterococcus durans KLDS6.0930 using whole genome sequencing and oral toxicity study.","volume":"9","author":"Li","year":"","journal-title":"Front. Microbiol."},{"key":"B60","doi-asserted-by":"publisher","DOI":"10.1186\/1471-2180-12-23","article-title":"The tyrosyl-tRNA synthetase like gene located in the tyramine biosynthesis cluster of Enterococcus durans is transcriptionally regulated by tyrosine concentration and extracellular pH.","volume":"12","author":"Linares","year":"2012","journal-title":"BMC Microbiol."},{"key":"B61","doi-asserted-by":"publisher","first-page":"49","DOI":"10.1016\/J.JBIOTEC.2015.11.012","article-title":"Complete genome sequence of Enterococcus durans KLDS6.0930, a strain with probiotic properties.","volume":"217","author":"Liu","year":"2016","journal-title":"J. Biotechnol."},{"key":"B62","article-title":"\u201cFamily IV. Enterococcaceae fam. nov.,\u201d","author":"Ludwig","year":"2009","journal-title":"Bergey\u2019s Manual of Systematic Bacteriology, The Firmicutes"},{"key":"B63","doi-asserted-by":"publisher","first-page":"1843","DOI":"10.1126\/science.1165771","article-title":"CRISPR interference limits horizontal gene transfer in Staphylococci by targeting DNA.","volume":"322","author":"Marraffini","year":"2008","journal-title":"Science"},{"key":"B64","doi-asserted-by":"publisher","first-page":"W182","DOI":"10.1093\/nar\/gkm321","article-title":"KAAS: an automatic genome annotation and pathway reconstruction server.","volume":"35","author":"Moriya","year":"2007","journal-title":"Nucleic Acids Res."},{"key":"B65","doi-asserted-by":"publisher","first-page":"710","DOI":"10.1056\/NEJM200003093421007","article-title":"Vancomycin-resistant Enterococcal infections.","volume":"342","author":"Murray","year":"2000","journal-title":"N. Engl. J. Med."},{"key":"B66","doi-asserted-by":"publisher","first-page":"328","DOI":"10.1046\/j.1525-1381.1999.99241.x","article-title":"Enterococci: new aspects of an old organism.","volume":"111","author":"Murray","year":"1999","journal-title":"Proc. Assoc. Am. Phys."},{"key":"B67","doi-asserted-by":"publisher","first-page":"1044","DOI":"10.1099\/jmm.0.074161-0","article-title":"Probiotic assessment of Enterococcus durans 6HL and Lactococcus lactis 2HL isolated from vaginal microflora.","volume":"63","author":"Nami","year":"2014","journal-title":"J. Med. Microbiol."},{"key":"B68","doi-asserted-by":"publisher","DOI":"10.3389\/fmicb.2019.00300","article-title":"Probiotic properties of Enterococcus isolated from artisanal dairy products.","volume":"10","author":"Nami","year":"2019","journal-title":"Front. Microbiol."},{"key":"B69","doi-asserted-by":"publisher","first-page":"43","DOI":"10.1016\/j.jgg.2014.07.002","article-title":"First complete genome sequence of a probiotic Enterococcus faecium strain T-110 and its comparative genome analysis with pathogenic and non-pathogenic Enterococcus faecium genomes.","volume":"42","author":"Natarajan","year":"2015","journal-title":"J. Genet. Genom."},{"key":"B70","doi-asserted-by":"publisher","first-page":"2071","DOI":"10.1126\/SCIENCE.1080613","article-title":"Role of mobile DNA in the evolution of vancomycin-resistant Enterococcus faecalis.","volume":"299","author":"Paulsen","year":"2003","journal-title":"Science"},{"key":"B71","doi-asserted-by":"publisher","first-page":"251","DOI":"10.1016\/j.foodcont.2013.09.055","article-title":"Probiotic potential, antimicrobial and antioxidant activities of Enterococcus durans strain LAB18s.","volume":"37","author":"Pieniz","year":"2014","journal-title":"Food Control"},{"key":"B72","doi-asserted-by":"publisher","first-page":"2335","DOI":"10.2741\/1400","article-title":"Enterococcal virulence - pathogenicity island of Enterococcus faecalis.","volume":"9","author":"Pillar","year":"2004","journal-title":"Front. Biosci."},{"key":"B73","doi-asserted-by":"publisher","DOI":"10.1186\/1471-2180-12-135","article-title":"Complete genome sequence of Enterococcus faecium strain TX16 and comparative genomic analysis of Enterococcus faecium genomes.","volume":"12","author":"Qin","year":"2012","journal-title":"BMC Microbiol."},{"key":"B74","doi-asserted-by":"publisher","first-page":"22","DOI":"10.1016\/J.FM.2013.03.010","article-title":"Strain-specific probiotics properties of Lactobacillus fermentum, Lactobacillus plantarum and Lactobacillus brevis isolates from Brazilian food products.","volume":"36","author":"Ramos","year":"2013","journal-title":"Food Microbiol."},{"key":"B75","doi-asserted-by":"publisher","first-page":"29","DOI":"10.1111\/j.1574-6968.1980.tb05599.x","article-title":"Adherence of bacteria to hydrocarbons: a simple method for measuring cell-surface hydrophobicity.","volume":"9","author":"Rosenberg","year":"1980","journal-title":"FEMS Microbiol. Lett."},{"key":"B76","doi-asserted-by":"crossref","first-page":"364","DOI":"10.1016\/S0022-2275(20)36896-6","article-title":"Determination of cholesterol using o-phthalaldehyde.","volume":"14","author":"Rudel","year":"1973","journal-title":"J. Lipid Res."},{"key":"B77","doi-asserted-by":"publisher","first-page":"2220","DOI":"10.1128\/JCM.02596-05","article-title":"Multilocus sequence typing scheme for Enterococcus faecalis reveals hospital-adapted genetic complexes in a background of high rates of recombination.","volume":"44","author":"Ruiz-Garbajosa","year":"2006","journal-title":"J. Clin. Microbiol."},{"key":"B78","doi-asserted-by":"publisher","first-page":"563","DOI":"10.1016\/S0958-6946(98)00077-6","article-title":"Clinical applications of probiotic bacteria.","volume":"8","author":"Salminen","year":"1998","journal-title":"Int. Dairy J."},{"key":"B79","doi-asserted-by":"publisher","first-page":"164","DOI":"10.4161\/gmic.1.3.12127","article-title":"Safety assessment of probiotics for human use.","volume":"1","author":"Sanders","year":"2010","journal-title":"Gut Microb."},{"key":"B80","doi-asserted-by":"publisher","first-page":"459","DOI":"10.1016\/J.IDAIRYJ.2010.02.005","article-title":"Evaluation of metabolic activities of Enterococci isolated from pecorino abruzzese cheese.","volume":"20","author":"Serio","year":"2010","journal-title":"Int. Dairy J."},{"key":"B81","doi-asserted-by":"publisher","first-page":"415","DOI":"10.1128\/iai.59.1.415-420.1991","article-title":"Nucleotide sequence of the gelatinase gene (gelE) from Enterococcus faecalis subsp. liquefaciens.","volume":"59","author":"Su","year":"1991","journal-title":"Infect. Immun."},{"key":"B82","doi-asserted-by":"publisher","first-page":"17","DOI":"10.17470\/NF-019-0003","article-title":"Enterococcus as probiotics: what is the advantage?","volume":"1","author":"Suvorov","year":"2019","journal-title":"Nutrafoods"},{"key":"B83","first-page":"201","article-title":"Changes in Parmesan cheese during ripening?: microflora - coliforms, Enterococci, anaerobes, Propionibacteria and Staphylococci.","volume":"41","author":"Thompson","year":"1986","journal-title":"Milchwissenschaft"},{"key":"B84","doi-asserted-by":"publisher","first-page":"4332","DOI":"10.1172\/JCI43918","article-title":"Vancomycin-resistant Enterococcus domination of intestinal microbiota is enabled by antibiotic treatment in mice and precedes bloodstream invasion in humans.","volume":"120","author":"Ubeda","year":"2010","journal-title":"J. Clin. Invest."},{"key":"B85","doi-asserted-by":"publisher","first-page":"157","DOI":"10.1007\/s00203-010-0668-3","article-title":"Horizontal gene transfer amongst probiotic lactic acid bacteria and other intestinal microbiota: what are the possibilities? A review.","volume":"193","author":"van Reenen","year":"2011","journal-title":"Arch. Microbiol."},{"key":"B86","doi-asserted-by":"publisher","DOI":"10.1186\/1471-2164-11-239","article-title":"Pyrosequencing-based comparative genome analysis of the nosocomial pathogen Enterococcus faecium and identification of a large transferable pathogenicity island.","volume":"11","author":"van Schaik","year":"2010","journal-title":"BMC Genomics"},{"key":"B87","doi-asserted-by":"publisher","first-page":"956","DOI":"10.3168\/jds.S0022-0302(93)77422-6","article-title":"Relationship among bile tolerance, bile salt deconjugation, and assimilation of cholesterol by Lactobacillus acidophilus.","volume":"76","author":"Walker","year":"1993","journal-title":"J. Dairy Sci."},{"key":"B88","doi-asserted-by":"publisher","DOI":"10.1186\/s12866-015-0515-4","article-title":"In silico identification of bacteriocin gene clusters in the gastrointestinal tract, based on the Human Microbiome Project\u2019s reference genome database.","volume":"15","author":"Walsh","year":"2011","journal-title":"BMC Microbiol."},{"key":"B89","doi-asserted-by":"publisher","DOI":"10.3390\/nu9050521","article-title":"Antioxidant properties of probiotic bacteria.","volume":"9","author":"Wang","year":"2017","journal-title":"Nutrients"},{"key":"B90","doi-asserted-by":"publisher","first-page":"81","DOI":"10.1556\/EuJMI-D-14-00039","article-title":"Virulence genes in a probiotic Escherichia coli product with a recorded long history of safe use.","volume":"5","author":"Wassenaar","year":"2015","journal-title":"Eur. J. Microbiol. Immunol."},{"key":"B91","doi-asserted-by":"publisher","DOI":"10.1186\/1471-2334-11-80","article-title":"IS element IS16 as a molecular screening tool to identify hospital-associated strains of Enterococcus faecium.","volume":"11","author":"Werner","year":"2011","journal-title":"BMC Infect. Dis."},{"key":"B92","author":"Wikler","year":"2006","journal-title":"Methods for Dilution Antimicrobial Susceptibility Tests for Bacteria that Grow Aerobically: Approved Standard."},{"key":"B93","doi-asserted-by":"publisher","first-page":"434","DOI":"10.1111\/j.1472-765X.2009.02684.x","article-title":"Assessment of cell surface properties and adhesion potential of selected probiotic strains.","volume":"49","author":"Xu","year":"2009","journal-title":"Lett. Appl. Microbiol."},{"key":"B94","doi-asserted-by":"publisher","first-page":"95","DOI":"10.1016\/J.MICRES.2016.12.009","article-title":"Comparative genomic analysis of the genus Enterococcus.","volume":"196","author":"Zhong","year":"2017","journal-title":"Microbiol. Res."}],"container-title":["Frontiers in Microbiology"],"original-title":[],"link":[{"URL":"https:\/\/www.frontiersin.org\/articles\/10.3389\/fmicb.2020.608314\/full","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2021,4,17]],"date-time":"2021-04-17T06:59:39Z","timestamp":1618642779000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.frontiersin.org\/articles\/10.3389\/fmicb.2020.608314\/full"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2020,12,11]]},"references-count":94,"alternative-id":["10.3389\/fmicb.2020.608314"],"URL":"https:\/\/doi.org\/10.3389\/fmicb.2020.608314","relation":{},"ISSN":["1664-302X"],"issn-type":[{"value":"1664-302X","type":"electronic"}],"subject":[],"published":{"date-parts":[[2020,12,11]]},"article-number":"608314"}}