{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,1]],"date-time":"2026-10-01T21:04:31Z","timestamp":1790888671566,"version":"4.1.0"},"reference-count":72,"publisher":"Frontiers Media SA","license":[{"start":{"date-parts":[[2020,12,8]],"date-time":"2020-12-08T00:00:00Z","timestamp":1607385600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100003074","name":"Agencia Nacional de Promoci\u00f3n Cient\u00edfica y Tecnol\u00f3gica","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100003074","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001691","name":"Japan Society for the Promotion of Science","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100001691","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["frontiersin.org"],"crossmark-restriction":true},"short-container-title":["Front. Microbiol."],"abstract":"<jats:p>Potential probiotic or immunobiotic effects of lactic acid bacteria (LAB) isolated from the milk of the South American camelid llama (<jats:italic>Lama glama<\/jats:italic>) have not been reported in published studies. The aim of the present work was to isolate beneficial LAB from llama milk that can be used as potential probiotics active against bacterial pathogens. LAB strains were isolated from llama milk samples. <jats:italic>In vitro<\/jats:italic> functional characterization of the strains was performed by evaluating the resistance against gastrointestinal conditions and inhibition of the pathogen growth. Additionally, the adhesive and immunomodulatory properties of the strains were assessed. The functional studies were complemented with a comparative genomic evaluation and <jats:italic>in vivo<\/jats:italic> studies in mice. <jats:italic>Ligilactobacillus salivarius<\/jats:italic> TUCO-L2 showed enhanced probiotic\/immunobiotic potential compared to that of other tested strains. The TUCO-L2 strain was resistant to pH and high bile salt concentrations and demonstrated antimicrobial activity against Gram-negative intestinal pathogens and adhesion to mucins and epithelial cells. <jats:italic>L. salivarius<\/jats:italic> TUCO-L2 modulated the innate immune response triggered by Toll-like receptor (TLR)-4 activation in intestinal epithelial cells. This effect involved differential regulation of the expression of inflammatory cytokines and chemokines mediated by the modulation of the negative regulators of the TLR signaling pathway. Moreover, the TUCO-L2 strain enhanced the resistance of mice to <jats:italic>Salmonella<\/jats:italic> infection. This is the first report on the isolation and characterization of a potential probiotic\/immunobiotic strain from llama milk. The <jats:italic>in vitro, in vivo<\/jats:italic>, and <jats:italic>in silico<\/jats:italic> investigation performed in this study reveals several research directions that are needed to characterize the TUCO-L2 strain in detail to position this strain as a probiotic or immunobiotic that can be used against infections in humans or animals, including llama.<\/jats:p>","DOI":"10.3389\/fmicb.2020.608752","type":"journal-article","created":{"date-parts":[[2020,12,8]],"date-time":"2020-12-08T04:54:39Z","timestamp":1607403279000},"update-policy":"https:\/\/doi.org\/10.3389\/crossmark-policy","source":"Crossref","is-referenced-by-count":23,"title":["Functional and Genomic Characterization of Ligilactobacillus salivarius TUCO-L2 Isolated From Lama glama Milk: A Promising Immunobiotic Strain to Combat Infections"],"prefix":"10.3389","volume":"11","author":[{"given":"Sandra","family":"Quilodr\u00e1n-Vega","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Leonardo","family":"Albarracin","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Flavia","family":"Mansilla","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lorena","family":"Arce","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Binghui","family":"Zhou","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Md Aminul","family":"Islam","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mikado","family":"Tomokiyo","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Imad","family":"Al Kassaa","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yoshihito","family":"Suda","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Haruki","family":"Kitazawa","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Julio","family":"Villena","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1965","published-online":{"date-parts":[[2020,12,8]]},"reference":[{"key":"B1","doi-asserted-by":"publisher","first-page":"270","DOI":"10.1134\/S0003683813030174","article-title":"Inhibition of foodborne pathogens by a bacteriocin-like substance produced by a novel strain of Lactobacillus acidophilus isolated from camel milk","volume":"49","author":"Abo-Amer","year":"2013","journal-title":"Appl. Biochem. Microbiol."},{"key":"B2","doi-asserted-by":"publisher","first-page":"543","DOI":"10.3389\/fimmu.2020.00543","article-title":"Efficient selection of new immunobiotic strains with antiviral effects in local and distal mucosal sites by using porcine intestinal epitheliocytes","volume":"11","author":"Albarracin","year":"","journal-title":"Front. Immunol."},{"key":"B3","doi-asserted-by":"publisher","first-page":"57","DOI":"10.3389\/fimmu.2017.00057","article-title":"Transcriptomic analysis of the innate antiviral immune response in porcine intestinal epithelial cells: influence of immunobiotic lactobacilli","volume":"8","author":"Albarracin","year":"2017","journal-title":"Front. Immunol."},{"key":"B4","doi-asserted-by":"publisher","first-page":"e00784","DOI":"10.1128\/MRA.00784-20","article-title":"Draft genome sequence of Ligilactobacillus salivarius TUCO-L2, isolated from Lama glama milk","volume":"9","author":"Albarracin","year":"","journal-title":"Microbiol. Resour. Announc."},{"key":"B5","doi-asserted-by":"publisher","first-page":"1516","DOI":"10.1128\/AEM.02176-10","article-title":"Influence of two-component signal transduction systems of Lactobacillus casei BL23 on tolerance to stress conditions","volume":"77","author":"Alc\u00e1ntara","year":"2011","journal-title":"Appl. Environ. Microbiol."},{"key":"B6","doi-asserted-by":"publisher","first-page":"677","DOI":"10.1016\/j.it.2018.04.002","article-title":"The intestinal epithelium: central coordinator of mucosal immunity","volume":"39","author":"Allaire","year":"2018","journal-title":"Trends Immunol."},{"key":"B7","doi-asserted-by":"publisher","first-page":"e00975","DOI":"10.1128\/MRA.00975-18","article-title":"Draft genome sequences of Lactobacillus salivarius A3iob and Lactobacillus johnsonii CRL1647, novel potential probiotic strains for honeybees (Apis mellifera L.)","volume":"7","author":"Audisio","year":"2018","journal-title":"Microbiol. Resour. Announc."},{"key":"B8","doi-asserted-by":"publisher","first-page":"1674","DOI":"10.1016\/j.bbamcr.2013.10.019","article-title":"Selective transport by SecA2: an expanding family of customized motor proteins","volume":"1843","author":"Bensing","year":"2014","journal-title":"Biochim. Biophys. Acta"},{"key":"B9","doi-asserted-by":"publisher","first-page":"1035","DOI":"10.1002\/cphy.c120027","article-title":"Bile formation and secretion","volume":"3","author":"Boyer","year":"2013","journal-title":"Compr. Physiol."},{"key":"B10","doi-asserted-by":"publisher","first-page":"225","DOI":"10.1590\/S1517-83822011000100028","article-title":"Correlation between API 50 CH and multiplex polymerase chain reaction for the identification of vaginal lactobacilli in isolates","volume":"42","author":"Brolazo","year":"2011","journal-title":"Braz. J. Microbiol"},{"key":"B11","doi-asserted-by":"publisher","first-page":"273","DOI":"10.3389\/fmicb.2018.00273","article-title":"Identification of new factors modulating adhesion abilities of the pioneer commensal bacterium Streptococcus salivarius","volume":"9","author":"Couvigny","year":"2018","journal-title":"Front. Microbiol."},{"key":"B12","doi-asserted-by":"publisher","first-page":"3579","DOI":"10.1111\/1462-2920.13853","article-title":"Three glycosylated serine-rich repeat proteins play a pivotal role in adhesion and colonization of the pioneer commensal bacterium, Streptococcus salivarius","volume":"19","author":"Couvigny","year":"2017","journal-title":"Environ. Microbiol."},{"key":"B13","doi-asserted-by":"publisher","first-page":"589","DOI":"10.1016\/S0309-1740(03)00174-8","article-title":"Meat and carcass quality from Peruvian llama (Lama glama) and alpaca (Lama pacos)","volume":"66","author":"Cristofanelli","year":"2004","journal-title":"Meat. Sci."},{"key":"B14","doi-asserted-by":"publisher","first-page":"107674","DOI":"10.1016\/j.celrep.2020.107674","article-title":"Lactobacilli have a niche in the human nose","volume":"31","author":"De Boeck","year":"2020","journal-title":"Cell Rep."},{"key":"B15","doi-asserted-by":"publisher","first-page":"6421","DOI":"10.3168\/jds.2012-5522","article-title":"Factors determining milk quality and implications for production structure under somatic cell count standard modification","volume":"95","author":"Dong","year":"2012","journal-title":"J. Dairy Sci."},{"key":"B16","doi-asserted-by":"publisher","first-page":"1792","DOI":"10.1093\/nar\/gkh340","article-title":"MUSCLE: multiple sequence alignment with high accuracy and high throughput","volume":"32","author":"Edgar","year":"2004","journal-title":"Nucleic Acids Res."},{"key":"B17","doi-asserted-by":"publisher","first-page":"12553","DOI":"10.1038\/s41598-018-31006-3","article-title":"In vitro and in vivo evidences for innate immune stimulators lactic acid bacterial starters isolated from fermented camel dairy products","volume":"8","author":"Elbanna","year":"2018","journal-title":"Sci. Rep."},{"key":"B18","doi-asserted-by":"publisher","first-page":"23","DOI":"10.1016\/j.sbi.2014.07.002","article-title":"Structural insights into bacterial recognition of intestinal mucins","volume":"28","author":"Etzold","year":"2014","journal-title":"Curr. Opin. Struct. Biol."},{"key":"B19","doi-asserted-by":"publisher","first-page":"888","DOI":"10.1111\/1462-2920.12377","article-title":"Structural basis for adaptation of lactobacilli to gastrointestinal mucus","volume":"16","author":"Etzold","year":"2014","journal-title":"Environ. Microbiol."},{"key":"B20","unstructured":"2019"},{"key":"B21","doi-asserted-by":"publisher","first-page":"779","DOI":"10.1038\/nrmicro2874","article-title":"Emerging themes in SecA2-mediated protein export","volume":"10","author":"Feltcher","year":"2012","journal-title":"Nat. Rev. Microbiol."},{"key":"B22","doi-asserted-by":"publisher","first-page":"e1004057","DOI":"10.1371\/journal.pgen.1004057","article-title":"Molecular characterization of host-specific biofilm formation in a vertebrate gut symbiont","volume":"9","author":"Frese","year":"2013","journal-title":"PLoS Genet."},{"key":"B23","doi-asserted-by":"publisher","first-page":"578","DOI":"10.1002\/9781118534168.ch26","article-title":"Camel Milk,","author":"Fukuda","year":"2013","journal-title":"Milk and Dairy Products in Human Nutrition"},{"key":"B24","doi-asserted-by":"publisher","first-page":"1376","DOI":"10.3389\/fimmu.2019.01376","article-title":"Evaluation of the immunomodulatory activities of the probiotic strain Lactobacillus fermentum UCO-979C","volume":"10","author":"Garcia-Castillo","year":"2019","journal-title":"Front. Immunol."},{"key":"B25","doi-asserted-by":"publisher","first-page":"1854","DOI":"10.3390\/ijms17111854","article-title":"Use of atomic force microscopy to study the multi-modular interaction of bacterial adhesins to mucins","volume":"17","author":"Gunning","year":"2016","journal-title":"Int. J. Mol. Sci."},{"key":"B26","doi-asserted-by":"publisher","first-page":"e000115","DOI":"10.1099\/mgen.0.000115","article-title":"Phylogenomics and comparative genomics of Lactobacillus salivarius, a mammalian gut commensal","volume":"3","author":"Harris","year":"2017","journal-title":"Microb. Genomics"},{"key":"B27","doi-asserted-by":"publisher","first-page":"169","DOI":"10.1186\/s12859-015-0611-3","article-title":"InteractiVenn: a web-based tool for the analysis of sets through Venn diagrams","volume":"16","author":"Heberle","year":"2015","journal-title":"BMC Bioinformatics"},{"key":"B28","doi-asserted-by":"publisher","first-page":"371","DOI":"10.1016\/j.lwt.2012.01.034","article-title":"Lactic acid bacteria diversity of African raw and fermented camel milk products reveals a highly competitive, potentially health-threatening predominant microflora","volume":"47","author":"Jans","year":"2012","journal-title":"LWT"},{"key":"B29","doi-asserted-by":"publisher","first-page":"17193","DOI":"10.1073\/pnas.0908876106","article-title":"Comparative genomic analysis of Lactobacillus rhamnosus GG reveals pili containing a human-mucus binding protein","volume":"106","author":"Kankainen","year":"2009","journal-title":"Proc. Natl. Acad. Sci. U.S.A."},{"key":"B30","doi-asserted-by":"publisher","first-page":"2178","DOI":"10.3389\/fimmu.2018.02178","article-title":"Genomic characterization of Lactobacillus delbrueckii TUA4408L and evaluation of the antiviral activities of its extracellular polysaccharides in porcine intestinal epithelial cells","volume":"9","author":"Kanmani","year":"","journal-title":"Front. Immunol."},{"key":"B31","doi-asserted-by":"publisher","first-page":"253","DOI":"10.1016\/j.molimm.2017.07.009","article-title":"Exopolysaccharides from Lactobacillus delbrueckii OLL1073R-1 modulate innate antiviral immune response in porcine intestinal epithelial cells","volume":"93","author":"Kanmani","year":"","journal-title":"Mol. Immunol."},{"key":"B32","doi-asserted-by":"publisher","first-page":"81","DOI":"10.1016\/j.micres.2006.10.008","article-title":"Characterization of lactic acid bacteria isolated from the one humped camel milk produced in Morocco","volume":"164","author":"Khedid","year":"2009","journal-title":"Microbiol. Res."},{"key":"B33","doi-asserted-by":"publisher","first-page":"769","DOI":"10.3920\/BM2016.0095","article-title":"Modulation of porcine intestinal epitheliocytes immunetranscriptome response by Lactobacillus jensenii TL2937","volume":"7","author":"Kobayashi","year":"2016","journal-title":"Benef. Microbes"},{"key":"B34","doi-asserted-by":"publisher","first-page":"357","DOI":"10.1038\/mi.2017.55","article-title":"Bacteroidales recruit IL-6-producing intraepithelial lymphocytes in the colon to promote barrier integrity","volume":"11","author":"Kuhn","year":"2018","journal-title":"Mucosal. Immunol."},{"key":"B35","doi-asserted-by":"publisher","first-page":"1547","DOI":"10.1093\/molbev\/msy096","article-title":"MEGA X: molecular evolutionary genetics analysis across computing platforms","volume":"35","author":"Kumar","year":"2018","journal-title":"Mol. Biol. Evol."},{"key":"B36","doi-asserted-by":"publisher","first-page":"848","DOI":"10.15381\/rivep.v29i3.14015","article-title":"Alpaca cheese: a new alternative","volume":"29","author":"Larico","year":"2018","journal-title":"Rev. Investig. Vet. del Peru"},{"key":"B37","doi-asserted-by":"publisher","first-page":"136","DOI":"10.3390\/ijms19010136","article-title":"How sweet are our gut beneficial bacteria? A focus on protein glycosylation","volume":"19","author":"Latousakis","year":"2018","journal-title":"Lactobacillus. Int. J. Mol. Sci."},{"key":"B38","doi-asserted-by":"publisher","first-page":"48","DOI":"10.1016\/j.micres.2017.08.008","article-title":"Comparative genomics of Lactobacillus salivarius strains focusing on their host adaptation","volume":"205","author":"Lee","year":"2017","journal-title":"Microbiol. Res."},{"key":"B39","doi-asserted-by":"publisher","first-page":"167","DOI":"10.1128\/CMR.00080-13","article-title":"Anti-infective activities of Lactobacillus strains in the human intestinal microbiota: From probiotics to gastrointestinal anti- infectious biotherapeutic agents","volume":"27","author":"Li\u00e9vin-Le Moal","year":"2014","journal-title":"Clin. Microbiol. Rev."},{"key":"B40","doi-asserted-by":"publisher","first-page":"276","DOI":"10.1016\/j.foodcont.2014.03.020","article-title":"Purification and partial characterization of a novel bacteriocin produced by Lactobacillus casei TN-2 isolated from fermented camel milk (Shubat) of Xinjiang Uygur Autonomous region, China","volume":"43","author":"L\u00fc","year":"2014","journal-title":"Food Control"},{"key":"B41","doi-asserted-by":"publisher","first-page":"15611","DOI":"10.1073\/pnas.0607117103","article-title":"Comparative genomics of the lactic acid bacteria","volume":"103","author":"Makarova","year":"2006","journal-title":"Proc. Natl. Acad. Sci. U.S.A."},{"key":"B42","doi-asserted-by":"publisher","first-page":"4539","DOI":"10.1128\/AEM.00284-08","article-title":"Analysis of vaginal lactobacilli from healthy and infected Brazilian women","volume":"74","author":"Martinez","year":"2008","journal-title":"Appl. Environ. Microbiol."},{"key":"B43","doi-asserted-by":"publisher","first-page":"167","DOI":"10.3390\/microorganisms7060167","article-title":"Isolation and immunocharacterization of Lactobacillus salivarius from the intestine of wakame-fed pigs to develop novel \u201cimmunosynbiotics.\u201d","volume":"7","author":"Masumizu","year":"2019","journal-title":"Microorganisms"},{"key":"B44","doi-asserted-by":"publisher","first-page":"6463","DOI":"10.1007\/s00253-019-09978-7","article-title":"Adhesion mechanisms mediated by probiotics and prebiotics and their potential impact on human health","volume":"103","author":"Monteagudo-Mera","year":"2019","journal-title":"Appl. Microbiol. Biotechnol"},{"key":"B45","doi-asserted-by":"publisher","first-page":"134","DOI":"10.1016\/j.bbagen.2007.11.006","article-title":"Toll-like receptor 4 and cytokine expression involved in functional immune response in an originally established porcine intestinal epitheliocyte cell line","volume":"1780","author":"Moue","year":"2008","journal-title":"Biochim. Biophys. Acta"},{"key":"B46","doi-asserted-by":"publisher","first-page":"861","DOI":"10.1111\/j.1365-2958.2004.04025.x","article-title":"A suf operon requirement for Fe-S cluster assembly during iron starvation in Escherichia coli","volume":"52","author":"Outten","year":"2004","journal-title":"Mol. Microbiol."},{"key":"B47","doi-asserted-by":"publisher","first-page":"3691","DOI":"10.1093\/bioinformatics\/btv421","article-title":"Roary: rapid large-scale prokaryote pan genome analysis","volume":"31","author":"Page","year":"2015","journal-title":"Bioinformatics"},{"key":"B48","doi-asserted-by":"publisher","first-page":"e0124963","DOI":"10.1371\/journal.pone.0124963","article-title":"Molecular characterization of the llamas (Lama glama) casein cluster genes transcripts (CSN1S1, CSN2, CSN1S2, CSN3) and regulatory regions","volume":"10","author":"Pauciullo","year":"2015","journal-title":"PLoS ONE"},{"key":"B49","doi-asserted-by":"publisher","first-page":"93","DOI":"10.1016\/S0921-4488(99)00127-3","article-title":"Carcass characteristics of llamas (Lama glama) reared in Central Chile","volume":"37","author":"P\u00e9rez","year":"2000","journal-title":"Small Rumin. Res."},{"key":"B50","doi-asserted-by":"publisher","first-page":"356","DOI":"10.1016\/j.meatsci.2006.07.010","article-title":"Meat fatty acid composition of llama (Lama glama) reared in the Andean highlands","volume":"75","author":"Polidori","year":"2007","journal-title":"Meat Sci."},{"key":"B51","doi-asserted-by":"publisher","first-page":"514","DOI":"10.1139\/cjm-2015-0811","article-title":"Isolation of lactic acid bacteria from swine milk and characterization of potential probiotic strains with antagonistic effects against swine-associated gastrointestinal pathogens","volume":"62","author":"Quilodr\u00e1n-Vega","year":"2016","journal-title":"Can. J. Microbiol."},{"key":"B52","doi-asserted-by":"publisher","first-page":"954","DOI":"10.1128\/AEM.01687-10","article-title":"Genomic diversity of Lactobacillus salivarius","volume":"77","author":"Raftis","year":"2011","journal-title":"Appl. Environ. Microbiol."},{"key":"B53","doi-asserted-by":"publisher","first-page":"100560","DOI":"10.1016\/j.nmni.2019.100560","article-title":"Distribution and antimicrobial activity of lactic acid bacteria from raw camel milk","volume":"30","author":"Rahmeh","year":"2019","journal-title":"New Microbes New Infect."},{"key":"B54","first-page":"406","article-title":"The neighbor-joining method: a new method for reconstructing phylogenetic trees","volume":"4","author":"Saitou","year":"1987","journal-title":"Mol. Biol. Evol."},{"key":"B55","doi-asserted-by":"publisher","first-page":"402","DOI":"10.3389\/fmicb.2015.00402","article-title":"The pho regulon: a huge regulatory network in bacteria","volume":"6","author":"Santos-Beneit","year":"2015","journal-title":"Front. Microbiol."},{"key":"B56","doi-asserted-by":"publisher","first-page":"2068","DOI":"10.1093\/bioinformatics\/btu153","article-title":"Prokka: rapid prokaryotic genome annotation","volume":"30","author":"Seemann","year":"2014","journal-title":"Bioinformatics"},{"key":"B57","first-page":"796","article-title":"Camel milk for food allergies in children","volume":"7","author":"Shabo","year":"2005","journal-title":"Isr. Med. Assoc. J."},{"key":"B58","doi-asserted-by":"publisher","first-page":"276","DOI":"10.1128\/IAI.05729-11","article-title":"Immunobiotic Lactobacillus jensenii elicits anti-inflammatory activity in porcine intestinal epithelial cells by modulating negative regulators of the toll-like receptor signaling pathway","volume":"80","author":"Shimazu","year":"2012","journal-title":"Infect. Immun."},{"key":"B59","doi-asserted-by":"publisher","first-page":"13249","DOI":"10.1073\/pnas.231473998","article-title":"IL-1\u03b2-converting enzyme (caspase-1) in intestinal inflammation","volume":"98","author":"Siegmund","year":"2001","journal-title":"Proc. Natl. Acad. Sci. U.S.A."},{"key":"B60","doi-asserted-by":"publisher","first-page":"1","DOI":"10.1186\/1471-2172-15-24","article-title":"Immunobiotic Lactobacillus jensenii as immune-health promoting factor to improve growth performance and productivity in post-weaning pigs","volume":"15","author":"Suda","year":"2014","journal-title":"BMC Immunol."},{"key":"B61","doi-asserted-by":"publisher","first-page":"11030","DOI":"10.1073\/pnas.0404206101","article-title":"Prospects for inferring very large phylogenies by using the neighbor-joining method","volume":"101","author":"Tamura","year":"2004","journal-title":"Proc. Natl. Acad. Sci. U.S.A."},{"key":"B62","doi-asserted-by":"publisher","first-page":"865","DOI":"10.3389\/fmicb.2017.00865","article-title":"Bacteriocin-producing Enterococcus faecium LCW 44: A high potential probiotic candidate from raw camel milk","volume":"8","author":"Vimont","year":"2017","journal-title":"Front. Microbiol."},{"key":"B63","doi-asserted-by":"publisher","first-page":"2080","DOI":"10.1002\/mnfr.201400218","article-title":"Lactobacillus delbrueckii TUA4408L and its extracellular polysaccharides attenuate enterotoxigenic Escherichia coli- induced inflammatory response in porcine intestinal epitheliocytes via toll-like receptor-2 and 4","volume":"58","author":"Wachi","year":"2014","journal-title":"Mol. Nutr. Food Res."},{"key":"B64","doi-asserted-by":"publisher","first-page":"1167","DOI":"10.1007\/s10295-015-1634-5","article-title":"Functional role of oppA encoding an oligopeptide-binding protein from Lactobacillus salivarius Ren in bile tolerance","volume":"42","author":"Wang","year":"2015","journal-title":"J. Ind. Microbiol. Biotechnol."},{"key":"B65","unstructured":"WarnesG. R.\n            BolkerB.\n            GorjancG.\n            GrothendieckG.\n            KorosecA.\n            LumleyT.\n          CRAN - Package gdata2017"},{"key":"B66","doi-asserted-by":"publisher","first-page":"1023","DOI":"10.1186\/s12864-015-2216-7","article-title":"The pan-genome of Lactobacillus reuteri strains originating from the pig gastrointestinal tract","volume":"16","author":"Wegmann","year":"2015","journal-title":"BMC Genomics"},{"key":"B67","doi-asserted-by":"publisher","first-page":"903","DOI":"10.1007\/s11250-019-02197-2","article-title":"Camelids: new players in the international animal production context","volume":"52","author":"Zarrin","year":"2020","journal-title":"Trop. Anim. Health Prod."},{"key":"B68","doi-asserted-by":"publisher","first-page":"221","DOI":"10.1039\/C9FO02116G","article-title":"Screening of: Lactobacillus salivarius strains from the feces of Chinese populations and the evaluation of their effects against intestinal inflammation in mice","volume":"11","author":"Zhai","year":"2020","journal-title":"Food Func."},{"key":"B69","doi-asserted-by":"publisher","first-page":"106","DOI":"10.3168\/jds.2019-17023","article-title":"Analyses of physicochemical properties, bacterial microbiota, and lactic acid bacteria of fresh camel milk collected in inner Mongolia","volume":"103","author":"Zhao","year":"2020","journal-title":"J. Dairy Sci."},{"key":"B70","doi-asserted-by":"publisher","first-page":"2782","DOI":"10.1099\/ijsem.0.004107","article-title":"A taxonomic note on the genus Lactobacillus: description of 23 novel genera, emended description of the genus Lactobacillus Beijerinck 1901, and union of Lactobacillaceae and Leuconostocaceae","volume":"70","author":"Zheng","year":"2020","journal-title":"Int. J. Syst. Evol. Microbiol."},{"key":"B71","doi-asserted-by":"publisher","first-page":"1659","DOI":"10.3390\/microorganisms8111659","article-title":"Selection of Immunobiotic Ligilactobacillus salivarius strains from the intestinal tract of wakame-fed pigs: functional and genomic studies","volume":"8","author":"Zhou","year":"2020","journal-title":"Microorganisms"},{"key":"B72","doi-asserted-by":"publisher","first-page":"286","DOI":"10.3389\/fmicb.2016.00286","article-title":"EPS-SJ exopolisaccharide produced by the strain Lactobacillus paracasei subsp. paracasei BGSJ2-8 is involved in adhesion to epithelial intestinal cells and decrease on E. coli association to Caco-2 cells","volume":"7","author":"\u017divkovi\u0107","year":"2016","journal-title":"Front. Microbiol."}],"container-title":["Frontiers in Microbiology"],"original-title":[],"link":[{"URL":"https:\/\/www.frontiersin.org\/articles\/10.3389\/fmicb.2020.608752\/full","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2020,12,8]],"date-time":"2020-12-08T04:54:52Z","timestamp":1607403292000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.frontiersin.org\/articles\/10.3389\/fmicb.2020.608752\/full"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2020,12,8]]},"references-count":72,"alternative-id":["10.3389\/fmicb.2020.608752"],"URL":"https:\/\/doi.org\/10.3389\/fmicb.2020.608752","relation":{},"ISSN":["1664-302X"],"issn-type":[{"value":"1664-302X","type":"electronic"}],"subject":[],"published":{"date-parts":[[2020,12,8]]},"article-number":"608752"}}