{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,12]],"date-time":"2026-09-12T11:49:34Z","timestamp":1789213774519,"version":"build-2803163510"},"reference-count":105,"publisher":"Frontiers Media SA","license":[{"start":{"date-parts":[[2021,1,21]],"date-time":"2021-01-21T00:00:00Z","timestamp":1611187200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100004901","name":"Funda\u00e7\u00e3o de Amparo \u00e0 Pesquisa do Estado de Minas Gerais","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100004901","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["frontiersin.org"],"crossmark-restriction":true},"short-container-title":["Front. Microbiol."],"abstract":"<jats:p>The combination of sulfadiazine and pyrimethamine plus folinic acid is the conventional treatment for congenital toxoplasmosis. However, this classical treatment presents teratogenic effects and bone marrow suppression. In this sense, new therapeutic strategies are necessary to reduce these effects and improve the control of infection. In this context, biogenic silver nanoparticles (AgNp-Bio) appear as a promising alternative since they have antimicrobial, antiviral, and antiparasitic activity. The purpose of this study to investigate the action of AgNp-Bio in BeWo cells, HTR-8\/SVneo cells and villous explants and its effects against <jats:italic>Toxoplasma gondii<\/jats:italic> infection. Both cells and villous explants were treated with different concentrations of AgNp-Bio or combination of sulfadiazine + pyrimethamine (SDZ + PYZ) in order to verify the viability. After, cells and villi were infected and treated with AgNp-Bio or SDZ + PYZ in different concentrations to ascertain the parasite proliferation and cytokine production profile. AgNp-Bio treatment did not reduce the cell viability and villous explants. Significant reduction was observed in parasite replication in both cells and villous explants treated with silver nanoparticles and classical treatment. The AgNp-Bio treatment increased of IL-4 and IL-10 by BeWo cells, while HTR8\/SVneo cells produced macrophage migration inhibitory factor (MIF) and IL-4. In the presence of <jats:italic>T. gondii<\/jats:italic>, the treatment induced high levels of MIF production by BeWo cells and IL-6 by HTR8SV\/neo. In villous explants, the AgNp-Bio treatment downregulated production of IL-4, IL-6, and IL-8 after infection. In conclusion, AgNp-Bio can decrease <jats:italic>T. gondii<\/jats:italic> infection in trophoblast cells and villous explants. Therefore, this treatment demonstrated the ability to reduce the <jats:italic>T. gondii<\/jats:italic> proliferation with induction of inflammatory mediators in the cells and independent of mediators in chorionic villus which we consider the use of AgNp-Bio promising in the treatment of toxoplasmosis in BeWo and HTR8\/SVneo cell models and in chorionic villi.<\/jats:p>","DOI":"10.3389\/fmicb.2020.623947","type":"journal-article","created":{"date-parts":[[2021,1,21]],"date-time":"2021-01-21T06:16:42Z","timestamp":1611209802000},"update-policy":"https:\/\/doi.org\/10.3389\/crossmark-policy","source":"Crossref","is-referenced-by-count":24,"title":["Biogenic Silver Nanoparticles Can Control Toxoplasma gondii Infection in Both Human Trophoblast Cells and Villous Explants"],"prefix":"10.3389","volume":"11","author":[{"given":"Idessania Nazareth","family":"Costa","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mayara","family":"Ribeiro","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Priscila","family":"Silva Franco","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rafaela Jos\u00e9","family":"da Silva","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Th\u00e1dia Evelyn","family":"de Ara\u00fajo","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Iliana Claudia Balga","family":"Mili\u00e1n","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Luana Carvalho","family":"Luz","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"P\u00e2mela Mendon\u00e7a","family":"Guirelli","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gerson","family":"Nakazato","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jos\u00e9 Roberto","family":"Mineo","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tiago W. 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Res."},{"key":"ref6","doi-asserted-by":"publisher","first-page":"e01866","DOI":"10.1128\/AAC.01866-16","article-title":"Genetic evidence for cytochrome b Qi site inhibition by 4 (1H)-quinolone-3-diarylethers and Antimycin in Toxoplasma gondii","volume":"61","author":"Alday","year":"2017","journal-title":"Antimicrob. Agents Chemother."},{"key":"ref7","first-page":"2705","article-title":"Antileishmanial effect of silver nanoparticles and their enhanced antiparasitic activity under ultraviolet light","volume-title":"Int. J. Nanomed.","author":"Allahverdiyev","year":"2011"},{"key":"ref8","doi-asserted-by":"publisher","first-page":"1559","DOI":"10.1007\/s00436-019-06257-2","article-title":"Susceptibility of human villous (BeWo) and extravillous (HTR-8\/SVneo) trophoblast cells to Toxoplasma gondii infection is modulated by intracellular iron availability","volume":"118","author":"Almeida","year":"2019","journal-title":"Parasitol. Res."},{"key":"ref9","doi-asserted-by":"publisher","first-page":"333","DOI":"10.1007\/s13205-017-0961-9","article-title":"Synthesis, characterization and biological applications of mycosynthesized silver nanoparticles","volume":"7","author":"Anbazhagan","year":"2017","journal-title":"3"},{"key":"ref10","doi-asserted-by":"publisher","first-page":"973","DOI":"10.1016\/j.placenta.2013.09.005","article-title":"Differential apoptosis in BeWo cells after infection with highly (RH) or moderately (ME49) virulent strains of Toxoplasma gondii is related to the cytokine profile secreted, the death receptor Fas expression and phosphorylated ERK1\/2 expression","volume":"34","author":"Angeloni","year":"2013","journal-title":"Placenta"},{"key":"ref11","doi-asserted-by":"publisher","first-page":"372","DOI":"10.3109\/21691401.2016.1160403","article-title":"Green synthesis of silver nanoparticles using Rheum palmatum root extract and their antibacterial activity against Staphylococcus aureus and Pseudomonas aeruginosa","volume":"45","author":"Arokiyaraj","year":"2017","journal-title":"Artif. Cells Nanomed. Biotechnol."},{"key":"ref12","first-page":"279","article-title":"Cytotoxicity and genotoxicity of silver nanoparticles in human cells","volume-title":"ACS Nano","author":"Asharani","year":"2009"},{"key":"ref13","doi-asserted-by":"publisher","first-page":"1603","DOI":"10.1007\/s00436-017-5458-2","article-title":"Nanomedicine advances in toxoplasmosis: diagnostic, treatment, and vaccine applications","volume":"116","author":"Assolini","year":"2017","journal-title":"Parasitol. Res."},{"key":"ref14","doi-asserted-by":"publisher","first-page":"407","DOI":"10.7883\/yoken.JJID.2014.422","article-title":"Severe toxoplasmic hepatitis in an immunocompetent patient","volume":"68","author":"Atilla","year":"2015","journal-title":"Jpn. J. Infect. 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Immunol."},{"key":"ref19","doi-asserted-by":"publisher","first-page":"151","DOI":"10.1007\/s001090050204","article-title":"Regulation of the immune response by macrophage migration inhibitory factor: biological and structural features","volume":"76","author":"Bernhagen","year":"1998","journal-title":"J. Mol. Med."},{"key":"ref20","first-page":"97","article-title":"Nanobiosciences: a contemporary approach in antiparasitic drugs","volume":"4","author":"Bhardwaj","year":"2012","journal-title":"Mol. Cell. Pharmacol."},{"key":"ref22","doi-asserted-by":"publisher","first-page":"248","DOI":"10.1016\/0003-2697(76)90527-3","article-title":"A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding","volume":"72","author":"Bradford","year":"1976","journal-title":"Anal. Biochem."},{"key":"ref23","doi-asserted-by":"publisher","first-page":"2193","DOI":"10.4049\/jimmunol.167.4.2193","article-title":"Toxoplasma gondii tachyzoites inhibit proinflammatory cytokine induction in infected macrophages by preventing nuclear translocation of the transcription factor NF-\u03baB","volume":"167","author":"Butcher","year":"2001","journal-title":"J. Immunol."},{"key":"ref24","doi-asserted-by":"publisher","first-page":"2848","DOI":"10.1128\/AEM.70.5.2848-2853.2004","article-title":"Use of aqueous silver to enhance inactivation of coliphage MS-2 by UV disinfection","volume":"70","author":"Butkus","year":"2004","journal-title":"Appl. Environ. 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Macromol."},{"key":"ref28","doi-asserted-by":"publisher","first-page":"240","DOI":"10.1016\/j.placenta.2012.12.006","article-title":"Trophoblast cells are able to regulate monocyte activity to control Toxoplasma gondii infection","volume":"34","author":"Castro","year":"2013","journal-title":"Placenta"},{"key":"ref29","doi-asserted-by":"publisher","first-page":"1","DOI":"10.1186\/1479-5876-12-132","article-title":"Azithromycin is able to control Toxoplasma gondii infection in human villous explants","volume":"132","author":"Castro-Filice","year":"2014","journal-title":"J. Transl. 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Microbiol."},{"key":"ref32","doi-asserted-by":"publisher","first-page":"473","DOI":"10.1016\/j.pt.2017.02.007","article-title":"The toxoplasma parasitophorous vacuole: an evolving host-parasite frontier","volume":"33","author":"Clough","year":"2017","journal-title":"Trends Parasitol."},{"key":"ref33","doi-asserted-by":"publisher","first-page":"884","DOI":"10.1016\/j.placenta.2009.08.002","article-title":"Azithromycin inhibits vertical transmission of Toxoplasma gondii in Calomys callosus (rodentia: cricetidae)","volume":"10","author":"Costa","year":"2009","journal-title":"Placenta"},{"key":"ref34","doi-asserted-by":"publisher","first-page":"7","DOI":"10.3389\/fcimb.2017.00340","article-title":"Enrofloxacin and Toltrazuril are able to reduce Toxoplasma gondii growth in human BeWo trophoblastic cells and villous explants from human third trimester pregnancy","volume":"26","author":"da Silva","year":"2017","journal-title":"Front. Cell. Infect. Microbiol."},{"key":"ref35","doi-asserted-by":"publisher","first-page":"121","DOI":"10.1007\/s13205-013-0130-8","article-title":"Extracellular synthesis of silver nanoparticles by the Bacillus strain CS 11 isolated from industrialized area","volume":"4","author":"Das","year":"2014","journal-title":"Biotech"},{"key":"ref36","doi-asserted-by":"publisher","first-page":"767","DOI":"10.1016\/j.colsurfb.2014.08.018","article-title":"Green synthesis, characterization and anti-inflammatory activity of silver nanoparticles using European black elderberry fruits extract","volume":"122","author":"David","year":"2014","journal-title":"Colloids Surf. B Bioint."},{"key":"ref37","doi-asserted-by":"publisher","first-page":"36","DOI":"10.1002\/cbin.11222","article-title":"Experimental models of maternal\u2013fetal interface and their potential use for nanotechnology applications","volume":"44","author":"de Ara\u00fajo","year":"2019","journal-title":"Cell Biol. Int."},{"key":"ref38","doi-asserted-by":"publisher","first-page":"30","DOI":"10.1016\/j.biopha.2016.03.004","article-title":"Utilization of nanoparticle technology in rheumatoid arthritis treatment","volume":"80","author":"Dolati","year":"2016","journal-title":"Biomed. Pharmacother."},{"key":"ref39","doi-asserted-by":"publisher","first-page":"1375","DOI":"10.1017\/S0031182012000765","article-title":"Toxoplasmosis in humans and animals in Brazil: high prevalence, high burden of disease, and epidemiology","volume":"139","author":"Dubey","year":"2012","journal-title":"Parasitology"},{"key":"ref40","doi-asserted-by":"publisher","first-page":"e00057","DOI":"10.1128\/CMR.00057-17","article-title":"Treatment of toxoplasmosis: historical perspective, animal models, and current clinical practice","volume":"31","author":"Dunay","year":"2018","journal-title":"Clin. Microbiol. Rev."},{"key":"ref41","doi-asserted-by":"publisher","first-page":"789","DOI":"10.1016\/j.nano.2015.11.016","article-title":"Silver nanoparticles: a new view on mechanistic aspects on antimicrobial activity","volume":"12","author":"Dur\u00e1n","year":"2016","journal-title":"Nanomedicine"},{"key":"ref42","doi-asserted-by":"publisher","first-page":"8","DOI":"10.1186\/1477-3155-3-8","article-title":"Mechanistic aspects of biosynthesis of silver nanoparticles by several Fusarium oxysporum strains","volume":"3","author":"Dur\u00e1n","year":"2005","journal-title":"J. Nanobiotechnology"},{"key":"ref44","doi-asserted-by":"publisher","first-page":"46","DOI":"10.1016\/j.actatropica.2017.10.027","article-title":"Biogenic silver nanoparticles inducing Leishmania amazonensis promastigote and amastigote death in vitro","volume":"178","author":"Fanti","year":"2018","journal-title":"Acta Trop."},{"key":"ref45","doi-asserted-by":"publisher","first-page":"50","DOI":"10.2353\/ajpath.2008.070432","article-title":"Macrophage migration inhibitory factor is upregulated in human first-trimester placenta stimulated by soluble antigen of Toxoplasma gondii, resulting in increased monocyte adhesion on villous explants","volume":"172","author":"Ferro","year":"2008","journal-title":"Am. J. Pathol."},{"key":"ref46","doi-asserted-by":"publisher","first-page":"3661","DOI":"10.1096\/fj.08-111666","article-title":"Macrophage migration inhibitory factor (MIF) is critical for the host resistance against Toxoplasma gondii","volume":"22","author":"Flores","year":"2008","journal-title":"FASEB J."},{"key":"ref47","doi-asserted-by":"publisher","first-page":"743","DOI":"10.1007\/s00204-010-0545-5","article-title":"Cytotoxicity and genotoxicity of silver nanoparticles in the human lung cancer cell line, A549","volume":"85","author":"Foldbjerg","year":"2011","journal-title":"Arch. Toxicol."},{"key":"ref48","doi-asserted-by":"publisher","first-page":"838","DOI":"10.1016\/j.placenta.2011.08.012","article-title":"Azithromycin and spiramycin induce anti-inflammatory response in human trophoblastic (BeWo) cells infected by Toxoplasma gondii but are able to control infection","volume":"32","author":"Franco","year":"2011","journal-title":"Placenta"},{"key":"ref49","first-page":"2655","article-title":"Experimental infection of Calomys callosus with atypical strains of toxoplasma gondii shows gender differences in severity of infection","volume-title":"Parasitol. Res.","author":"Franco","year":"2014"},{"key":"ref50","doi-asserted-by":"publisher","first-page":"30","DOI":"10.1016\/j.exppara.2014.05.005","article-title":"Chitosan and silver nanoparticles: promising anti-Toxoplasma agents","volume":"143","author":"Gaafar","year":"2014","journal-title":"Exp. Parasitol."},{"key":"ref51","doi-asserted-by":"publisher","first-page":"1","DOI":"10.1016\/j.colcom.2018.02.005","article-title":"Comparative stress physiology analysis of biologically and chemically synthesized silver nanoparticles on Solanum lycopersicum L","volume":"24","author":"Girilal","year":"2018","journal-title":"Colloids Surf. B Bioint."},{"key":"ref52","first-page":"2792","article-title":"Effect of macrophage migration inhibitory factor (MIF) in human placental explants infected with Toxoplasma gondii depends on gestational age","volume-title":"Am. J. Pathol.","author":"Gomes","year":"2011"},{"key":"ref53","doi-asserted-by":"publisher","first-page":"528","DOI":"10.1093\/molehr\/gat023","article-title":"Cytokine IL-6 secretion by trophoblasts regulated via sphingosine-1-phosphate receptor 2 involving rho\/rho-kinase and Rac1 signaling pathways","volume":"19","author":"Goyal","year":"2013","journal-title":"Mol. Hum. Reprod."},{"key":"ref54","doi-asserted-by":"publisher","first-page":"373","DOI":"10.1186\/1556-276X-9-373","article-title":"Enhanced antibacterial and anti-biofilm activities of silver nanoparticles against gram-negative and gram-positive bacteria","volume":"9","author":"Gurunathan","year":"2014","journal-title":"Nanoscale Res. Lett."},{"key":"ref56","doi-asserted-by":"publisher","first-page":"174","DOI":"10.1016\/j.molimm.2017.06.020","article-title":"The role of monocytes in models of infection by protozoan parasites","volume":"88","author":"Hu","year":"2017","journal-title":"Mol. Immunol."},{"key":"ref57","doi-asserted-by":"publisher","first-page":"975","DOI":"10.1016\/j.tiv.2005.06.034","article-title":"In vitro toxicity of nanoparticles in BRL 3A rat liver cells","volume":"19","author":"Hussain","year":"2005","journal-title":"Toxicol. In Vitro"},{"key":"ref58","doi-asserted-by":"publisher","first-page":"38","DOI":"10.1007\/s11274-018-2420-0","article-title":"Decanethiol functionalized silver nanoparticles are new powerful leishmanicidals in vitro","volume":"34","author":"Isaac-M\u00e1rquez","year":"2018","journal-title":"World J. Microbiol. Biotechnol."},{"key":"ref59","doi-asserted-by":"publisher","first-page":"231","DOI":"10.1046\/j.1365-3024.1998.00152.x","article-title":"A protective role for IL-6 during early infection with toxoplasma gondii","volume":"20","author":"Jebbari","year":"1998","journal-title":"Parasite Immunol."},{"key":"ref60","doi-asserted-by":"publisher","first-page":"1","DOI":"10.1016\/j.molbiopara.2009.11.005","article-title":"Characterization of a leucine aminopeptidase from Toxoplasma gondii","volume":"170","author":"Jia","year":"2010","journal-title":"Mol. Biochem. Parasitol."},{"key":"ref61","doi-asserted-by":"publisher","first-page":"1337","DOI":"10.1055\/s-0036-1582397","article-title":"Inflammation in complicated pregnancy and its outcome","volume":"33","author":"Kalagiri","year":"2016","journal-title":"Am. J. Perinatol."},{"key":"ref62","doi-asserted-by":"publisher","first-page":"353","DOI":"10.1016\/j.colsurfb.2012.06.024","article-title":"Pharmacokinetics of curcumin-loaded PLGA and PLGA\u2013PEG blend nanoparticles after oral administration in rats","volume":"101","author":"Khalil","year":"2013","journal-title":"Colloids Surf. B Bioint."},{"key":"ref63","doi-asserted-by":"publisher","first-page":"1163","DOI":"10.3390\/nano5031163","article-title":"Nanotoxicity: an interplay of oxidative stress, inflammation and cell death","volume":"5","author":"Khanna","year":"2015","journal-title":"Nano"},{"key":"ref65","doi-asserted-by":"publisher","first-page":"362","DOI":"10.1016\/j.jfo.2010.03.002","article-title":"Toxoplasma and pregnancy","volume":"33","author":"Kodjikian","year":"2010","journal-title":"J. Fr. Ophtalmol."},{"key":"ref66","doi-asserted-by":"publisher","first-page":"191","DOI":"10.1111\/j.1447-0756.2008.00963.x","article-title":"Toll-like receptors and pregnancy: trophoblast as modulators of the immune response","volume":"35","author":"Koga","year":"2009","journal-title":"J. Obstet. Gynaecol."},{"key":"ref67","doi-asserted-by":"publisher","first-page":"259","DOI":"10.1016\/S0924-8579(01)00390-9","article-title":"Birth outcome following maternal use of fluoroquinolones","volume":"18","author":"Larsen","year":"2001","journal-title":"Int. J. Antimicrob. Agents"},{"key":"ref68","doi-asserted-by":"publisher","first-page":"e97775","DOI":"10.1371\/journal.pone.0097775","article-title":"A meta-analysis on risk of adverse pregnancy outcomes in Toxoplasma gondii infection","volume":"9","author":"Li","year":"2014","journal-title":"PLoS One"},{"key":"ref69","doi-asserted-by":"publisher","first-page":"92","DOI":"10.1016\/j.cplett.2010.01.027","article-title":"Effect of surface coating on the toxicity of silver nanomaterials on human skin keratinocytes","volume":"487","author":"Lu","year":"2010","journal-title":"Chem. Phys. Lett."},{"key":"ref70","doi-asserted-by":"publisher","first-page":"107853","DOI":"10.1016\/j.exppara.2020.107853","article-title":"Biogenic silver nanoparticles reduce adherence, infection, and proliferation of Toxoplasma gondii RH strain in HeLa cells without inflammatory mediators induction","volume":"211","author":"Machado","year":"2020","journal-title":"Exp. Parasitol."},{"key":"ref71","doi-asserted-by":"publisher","first-page":"77","DOI":"10.1016\/j.preteyeres.2013.12.005","article-title":"Ocular toxoplasmosis past, present and new aspects of an old disease","volume":"39","author":"Maenz","year":"2014","journal-title":"Prog. Retin. Eye Res."},{"key":"ref72","doi-asserted-by":"publisher","first-page":"1535","DOI":"10.1515\/BC.2006.191","article-title":"Leucine aminopeptidases: diversity in structure and function","volume":"387","author":"Matsui","year":"2006","journal-title":"Biol. Chem."},{"key":"ref74","first-page":"852","article-title":"Increased Toxoplasma gondii intracellular proliferation in human Extravillous Trophoblast cells (HTR8\/ SVneo line) is sequentially triggered by MIF, ERK1\/2, and COX-2","volume-title":"Front. Microbiol.","author":"Milian","year":"2019"},{"key":"ref75","doi-asserted-by":"publisher","first-page":"439","DOI":"10.1016\/j.placenta.2004.10.002","article-title":"Human placental explants in culture: approaches and assessments","volume":"26","author":"Miller","year":"2005","journal-title":"Placenta"},{"key":"ref76","doi-asserted-by":"publisher","first-page":"13","DOI":"10.2217\/fmb.11.147","article-title":"IL-6 signaling SOCS critical for IL-12 host response to Toxoplasma gondii","volume":"7","author":"Mirpuri","year":"2012","journal-title":"Future Microbiol."},{"key":"ref500","doi-asserted-by":"publisher","first-page":"865","DOI":"10.1166\/jnn.2018.13966","article-title":"In vivo; in vitro interaction of silver nanoparticles with leucine aminopeptidase from human and Plasmodium falciparum","volume":"18","author":"Mnkandhla","year":"2018","journal-title":"J. Nanosci. Nanotechnol."},{"key":"ref77","doi-asserted-by":"publisher","first-page":"25","DOI":"10.3389\/fmicb.2017.00025","article-title":"A systematic review of in vitro and in vivo activities of anti-toxoplasma drugs na compounds","volume":"8","author":"Montazeri","year":"2017","journal-title":"Front. Microbiol."},{"key":"ref78","doi-asserted-by":"publisher","first-page":"554","DOI":"10.1086\/590149","article-title":"Management of Toxoplasma gondii infection during pregnancy","volume":"47","author":"Montoya","year":"2008","journal-title":"Clin. Infect. Dis."},{"key":"ref80","doi-asserted-by":"publisher","first-page":"55","DOI":"10.1016\/0022-1759(83)90303-4","article-title":"Rapid colorimetric assay for cellular growth and survival: application to proliferation and cytotoxicity assays","volume":"65","author":"Mosmann","year":"1983","journal-title":"J. Immunol. Methods"},{"key":"ref81","doi-asserted-by":"publisher","first-page":"17322","DOI":"10.1039\/C6NR06749B","article-title":"A 3D co-culture microtissue model of the human placenta for nanotoxicity assessment","volume":"8","author":"Muoth","year":"2016","journal-title":"Nanoscale"},{"key":"ref82","doi-asserted-by":"publisher","first-page":"823","DOI":"10.3390\/nano5031163","article-title":"Nanotoxicology: an emerging discipline evolving from studies of ultrafine particles","volume":"113","author":"Oberd\u00f6rster","year":"2005","journal-title":"Environ. Health Perspect."},{"key":"ref83","doi-asserted-by":"publisher","first-page":"691","DOI":"10.1016\/j.placenta.2005.06.006","article-title":"BeWo trophoblasts are unable to control replication of Toxoplasma gondii, even in the presence of exogenous IFN gamma","volume":"27","author":"Oliveira","year":"2006","journal-title":"Placenta"},{"key":"ref84","doi-asserted-by":"publisher","first-page":"385","DOI":"10.3389\/fmicb.2014.00385","article-title":"Maternal and congenital toxoplasmosis, currently available and novel therapies in horizon","volume":"5","author":"Oz","year":"2014","journal-title":"Front. Microbiol."},{"key":"ref86","doi-asserted-by":"publisher","first-page":"27021","DOI":"10.1007\/s11356-018-2620-z","article-title":"Inactivation of influenza a virus via exposure to silver nanoparticle-decorated silica hybrid composites","volume":"25","author":"Park","year":"2018","journal-title":"Environ. Sci. Pollut. Res. Int."},{"key":"ref88","doi-asserted-by":"publisher","first-page":"214","DOI":"10.1016\/j.siny.2007.01.011","article-title":"Toxoplasmosis","volume":"12","author":"Petersen","year":"2007","journal-title":"Semin. Fetal Neonatal Med."},{"key":"ref89","doi-asserted-by":"publisher","first-page":"e0005222","DOI":"10.1371\/journal.pntd.0005222","article-title":"Congenital toxoplasmosis in France and the United States: one parasite, two diverging approaches","volume":"11","author":"Peyron","year":"2017","journal-title":"PLoS Negl. Trop. Dis."},{"key":"ref90","doi-asserted-by":"publisher","first-page":"22","DOI":"10.1016\/j.jtemb.2015.11.004","article-title":"Biological production of silver nanoparticles by soil isolated bacteria and preliminary study of their cytotoxicity and cutaneous wound healing efficiency in rat","volume":"34","author":"Pourali","year":"2016","journal-title":"J. Trace Elem. Med. Biol."},{"key":"ref91","doi-asserted-by":"publisher","first-page":"1","DOI":"10.1016\/j.jri.2012.05.004","article-title":"Interleukin-6 in pregnancy and gestational disorders","volume":"95","author":"Prins","year":"2012","journal-title":"J. Reprod. Immunol."},{"key":"ref92","doi-asserted-by":"publisher","first-page":"353","DOI":"10.1016\/j.parint.2015.05.004","article-title":"In vitro antiparasitic activity of microbial pigments and their combination with phytosynthesized metal nanoparticles","volume":"64","author":"Rahul","year":"2015","journal-title":"Parasitol. Int."},{"key":"ref93","doi-asserted-by":"publisher","first-page":"872","DOI":"10.1002\/anie.200802585","article-title":"Nanomedicine: challenge and perspectives","volume":"48","author":"Riehemann","year":"2009","journal-title":"Angew. Chem. Int. Ed. Eng."},{"key":"ref94","doi-asserted-by":"publisher","first-page":"29","DOI":"10.1016\/J.jab.2017.10.009","article-title":"Proliferation of Toxoplasma gondii (RH strain) is inhibited by the combination of pravastatin and simvastatin with low concentrations of conventional drugs used in toxoplasmosis","volume":"16","author":"Sanfelice","year":"2018","journal-title":"J. Appl. Biomed."},{"key":"ref95","doi-asserted-by":"publisher","first-page":"75","DOI":"10.1016\/j.exppara.2017.07.009","article-title":"Activity of rosuvastatin in tachyzoites of Toxoplasma gondii (RH strain) in HeLa cells","volume":"181","author":"Sanfelice","year":"2017","journal-title":"Exp. Parasitol."},{"key":"ref96","doi-asserted-by":"publisher","first-page":"156","DOI":"10.5101\/nbe.v10i2.p165-173","article-title":"Extracellular biosynthesis of silver nanoparticles using fungi and their antibacterial activity","volume":"10","author":"Sangui\u00f1edo","year":"2018","journal-title":"Nano Biomed. Eng."},{"key":"ref97","doi-asserted-by":"publisher","first-page":"760","DOI":"10.3389\/fmicb.2016.00760","article-title":"Synergistic and additive effect of oregano essential oil and biological silver nanoparticles against multidrug-resistant bacterial strains","volume":"7","author":"Scandorieiro","year":"2016","journal-title":"Front. Microbiol."},{"key":"ref98","doi-asserted-by":"publisher","first-page":"186","DOI":"10.2174\/1574891x10666150410120321","article-title":"Anti-Toxoplasma activity of natural products: a review","volume":"9","author":"Sep\u00falveda-Arias","year":"2014","journal-title":"Recent Pat. Antiinfect. Drug Discov."},{"key":"ref99","doi-asserted-by":"publisher","first-page":"S66","DOI":"10.1086\/338000","article-title":"Suppression of NF-\u03baB activation by infection with Toxoplasma gondii","volume":"185","author":"Shapira","year":"2002","journal-title":"sis."},{"key":"ref100","doi-asserted-by":"publisher","first-page":"881","DOI":"10.1007\/s00253-018-9488-1","article-title":"Green synthesis of silver nanoparticles from medicinal plants and evaluation of their antiviral potential against chikungunya virus","volume":"103","author":"Sharma","year":"2019","journal-title":"Appl. Microbiol. Biotechnol."},{"key":"ref101","doi-asserted-by":"publisher","first-page":"141","DOI":"10.1016\/j.parint.2007.01.008","article-title":"Maternal-fetal transmission of Toxoplasma gondii in interferon-gamma deficient pregnant mice","volume":"56","author":"Shiono","year":"2007","journal-title":"Parasitol. Int."},{"key":"ref102","doi-asserted-by":"publisher","first-page":"225103","DOI":"10.1088\/0957-4484\/18\/22\/225103","article-title":"Characterization of enhanced antibacterial effects of novel silver nanoparticles","volume":"18","author":"Shrivastava","year":"2007","journal-title":"Nanotechnology"},{"key":"ref103","doi-asserted-by":"publisher","first-page":"473","DOI":"10.1016\/j.tiv.2014.01.008","article-title":"Silver nanoparticles induced changes in the expression of NF-\u03baB related genes are cell type specific and related to the basal activity of NF-\u03baB","volume":"28","author":"St\u0119pkowski","year":"2014","journal-title":"Toxicol. In Vitro"},{"key":"ref104","doi-asserted-by":"publisher","first-page":"161","DOI":"10.1111\/acps.12423","article-title":"Beyond the association Toxoplasma gondii in schizophrenia, bipolar disorder, and addiction: systematic review and meta-analysis","volume":"132","author":"Sutterland","year":"2015","journal-title":"Acta Psychiatr. Scand."},{"key":"ref105","doi-asserted-by":"publisher","first-page":"348","DOI":"10.1016\/j.exppara.2010.03.009","article-title":"Toxoplasma gondii: impaired maturation and pro-inflammatory response of dendritic cells in MIF-deficient mice favors susceptibility to infection","volume":"126","author":"Terrazas","year":"2010","journal-title":"Exp. Parasitol."},{"key":"ref106","doi-asserted-by":"publisher","first-page":"867","DOI":"10.3390\/molecules21070867","article-title":"Advanced nanobiomaterials: vaccines, diagnosis and treatment of infectious diseases","volume":"21","author":"Torres-Santiago","year":"2016","journal-title":"Molecules"},{"key":"ref107","doi-asserted-by":"publisher","first-page":"1099","DOI":"10.1016\/j.drudis.2018.01.048","article-title":"Nanomedicine as a potential approach to empower the new strategies for the treatment of preeclampsia","volume":"23","author":"Valero","year":"2018","journal-title":"Drug Discov. Today"},{"key":"ref108","doi-asserted-by":"publisher","first-page":"257","DOI":"10.1007\/s40089-016-0192-9","article-title":"Fungal mediated silver nanoparticle synthesis using robust experimental design and its application in cotton fabric","volume":"6","author":"Velhal","year":"2016","journal-title":"Int Nano Lett."},{"key":"ref109","doi-asserted-by":"publisher","first-page":"1129","DOI":"10.1002\/cmdc.200900049","article-title":"Further evidence of the anti-inflammatory effects of silver nanoparticles","volume":"4","author":"Wong","year":"2009","journal-title":"ChemMedChem"},{"key":"ref110","doi-asserted-by":"publisher","first-page":"109","DOI":"10.1038\/nri3598","article-title":"Innate immunity to Toxoplasma gondii infection","volume":"14","author":"Yarovinsky","year":"2014","journal-title":"Nat. Rev. Immunol."},{"key":"ref111","doi-asserted-by":"publisher","first-page":"1534","DOI":"10.3390\/ijms17091534","article-title":"Silver nanoparticles: synthesis, characterization, properties, applications, and therapeutic approaches","volume":"17","author":"Zhang","year":"2016","journal-title":"Int. J. Mol. Sci."},{"key":"ref112","doi-asserted-by":"publisher","first-page":"526","DOI":"10.1016\/j.talanta.2007.06.014","article-title":"Study on the interaction between silver nanoparticles and nucleic acids in the presence of cetyltrimethylammonium bromide and its analytical application","volume":"74","author":"Zheng","year":"2008","journal-title":"Talanta"}],"container-title":["Frontiers in Microbiology"],"original-title":[],"link":[{"URL":"https:\/\/www.frontiersin.org\/articles\/10.3389\/fmicb.2020.623947\/full","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2021,1,21]],"date-time":"2021-01-21T06:16:46Z","timestamp":1611209806000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.frontiersin.org\/articles\/10.3389\/fmicb.2020.623947\/full"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2021,1,21]]},"references-count":105,"alternative-id":["10.3389\/fmicb.2020.623947"],"URL":"https:\/\/doi.org\/10.3389\/fmicb.2020.623947","relation":{},"ISSN":["1664-302X"],"issn-type":[{"value":"1664-302X","type":"electronic"}],"subject":[],"published":{"date-parts":[[2021,1,21]]},"article-number":"623947"}}