{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,16]],"date-time":"2026-09-16T12:09:51Z","timestamp":1789560591260,"version":"build-2803163510"},"reference-count":30,"publisher":"Frontiers Media SA","license":[{"start":{"date-parts":[[2021,3,15]],"date-time":"2021-03-15T00:00:00Z","timestamp":1615766400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["frontiersin.org"],"crossmark-restriction":true},"short-container-title":["Front. Microbiol."],"abstract":"<jats:p>The emergence of carbapenem resistance (CR) caused by hydrolytic enzymes called carbapenemases has become a major concern worldwide. So far, CR genes have been widely detected in various bacteria. However, there is no report of CR gene harboring <jats:italic>Comamonas thiooxydans<\/jats:italic>. We first isolated a strain of an IMP-8-producing <jats:italic>C. thiooxydans<\/jats:italic> from a patient with urinary tract infection in China. Species identity was determined using MALDI-TOF MS analysis and carbapenemase-encoding genes were detected using PCR. The complete genomic sequence of <jats:italic>C. thiooxydans<\/jats:italic> was identified using Illumina Novaseq and Oxford Nanopore PromethION. Antimicrobial susceptibility analysis indicated that the <jats:italic>C. thiooxydans<\/jats:italic> strain ZDHYF418 was susceptible to imipenem, intermediate to meropenem, and was resistant to aztreonam, fluoroquinolones, and aminoglycosides. The <jats:italic>bla<\/jats:italic><jats:sub><jats:italic>IMP\u2013<\/jats:italic><\/jats:sub><jats:sub>8<\/jats:sub> gene was chromosomally located, and was part of a Tn402-like class 1 integron characterized by the following structure: <jats:italic>DDE-type integrase\/transposase\/recombinase-tniB-tniQ-recombinase family protein-aac(6\u2032)-Ib-cr-bla<jats:sub><jats:italic>IMP\u2013<\/jats:italic><\/jats:sub><jats:sub>8<\/jats:sub>-intI1<\/jats:italic>. Phylogenetic analysis demonstrated that the closest relative of ZDHYF418 is <jats:italic>C. thiooxydans<\/jats:italic> QYY (accession number: <jats:ext-link>CP053920.1<\/jats:ext-link>). We detected 330 SNP differences between ZDHYF418 and <jats:italic>C. thiooxydans<\/jats:italic> QYY. Strain QYY was isolated from activated sludge in Jilin province, China in 2015. In summary, we isolated a strain of <jats:italic>C. thiooxydans<\/jats:italic> that is able to produce IMP-8 and a novel <jats:italic>bla<\/jats:italic><jats:sub><jats:italic>OXA<\/jats:italic><\/jats:sub>. This is the first time that a CR gene has been identified in <jats:italic>C. thiooxydans<\/jats:italic>. The occurrence of the strain needs to be closely monitored.<\/jats:p>","DOI":"10.3389\/fmicb.2021.585716","type":"journal-article","created":{"date-parts":[[2021,3,15]],"date-time":"2021-03-15T04:28:10Z","timestamp":1615782490000},"update-policy":"https:\/\/doi.org\/10.3389\/crossmark-policy","source":"Crossref","is-referenced-by-count":16,"title":["Emergence of IMP-8-Producing Comamonas thiooxydans Causing Urinary Tract Infection in China"],"prefix":"10.3389","volume":"12","author":[{"given":"Xiaobing","family":"Guo","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Qian","family":"Wang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hao","family":"Xu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiaohong","family":"He","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lihua","family":"Guo","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shuxiu","family":"Liu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Peipei","family":"Wen","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jianjun","family":"Gou","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1965","published-online":{"date-parts":[[2021,3,15]]},"reference":[{"key":"B1","doi-asserted-by":"publisher","DOI":"10.1186\/1471-2164-9-75","article-title":"The RAST server: rapid annotations using subsystems technology.","volume":"9","author":"Aziz","year":"2008","journal-title":"BMC Genomics"},{"key":"B2","doi-asserted-by":"publisher","DOI":"10.1093\/femsle\/fnw166","article-title":"Mutations in NalC induce MexAB-OprM overexpression resulting in high level of aztreonam resistance in environmental isolates of Pseudomonas aeruginosa.","volume":"363","author":"Braz","year":"2016","journal-title":"FEMS Microbiol. 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