{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,1]],"date-time":"2026-09-01T04:56:06Z","timestamp":1788238566657,"version":"build-2803163510"},"reference-count":54,"publisher":"Frontiers Media SA","license":[{"start":{"date-parts":[[2021,2,10]],"date-time":"2021-02-10T00:00:00Z","timestamp":1612915200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100004573","name":"Ministry of Agriculture of the People's Republic of China","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100004573","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["31722044"],"award-info":[{"award-number":["31722044"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["frontiersin.org"],"crossmark-restriction":true},"short-container-title":["Front. Microbiol."],"abstract":"<jats:p>Protein lysine 2-hydroxyisobutyrylation (K<jats:sub><jats:italic>hib<\/jats:italic><\/jats:sub>), a new type of post-translational modification, occurs in histones and non-histone proteins and plays an important role in almost all aspects of both eukaryotic and prokaryotic living cells. <jats:italic>Fusarium oxysporum<\/jats:italic>, a soil-borne fungal pathogen, can cause disease in more than 150 plants. However, little is currently known about the functions of K<jats:sub><jats:italic>hib<\/jats:italic><\/jats:sub> in this plant pathogenic fungus. Here, we report a systematic analysis of 2-hydroxyisobutyrylated proteins in <jats:italic>F. oxysporum<\/jats:italic>. In this study, 3782 K<jats:sub><jats:italic>hib<\/jats:italic><\/jats:sub> sites in 1299 proteins were identified in <jats:italic>F. oxysporum<\/jats:italic>. The bioinformatics analysis showed that 2-hydroxyisobutyrylated proteins are involved in different biological processes and functions and are located in diverse subcellular localizations. The enrichment analysis revealed that K<jats:sub><jats:italic>hib<\/jats:italic><\/jats:sub> participates in a variety of pathways, including the ribosome, oxidative phosphorylation, and proteasome pathways. The protein interaction network analysis showed that 2-hydroxyisobutyrylated protein complexes are involved in diverse interactions. Notably, several 2-hydroxyisobutyrylated proteins, including three kinds of protein kinases, were involved in the virulence or conidiation of <jats:italic>F. oxysporum<\/jats:italic>, suggesting that K<jats:sub><jats:italic>hib<\/jats:italic><\/jats:sub> plays regulatory roles in pathogenesis. Moreover, our study shows that there are different K<jats:sub><jats:italic>hib<\/jats:italic><\/jats:sub> levels of <jats:italic>F. oxysporum<\/jats:italic> in conidial and mycelial stages. These findings provide evidence of K<jats:sub><jats:italic>hib<\/jats:italic><\/jats:sub> in <jats:italic>F. oxysporum<\/jats:italic>, an important filamentous plant pathogenic fungus, and serve as a resource for further exploration of the potential functions of K<jats:sub><jats:italic>hib<\/jats:italic><\/jats:sub> in <jats:italic>Fusarium<\/jats:italic> species and other filamentous pathogenic fungi.<\/jats:p>","DOI":"10.3389\/fmicb.2021.623735","type":"journal-article","created":{"date-parts":[[2021,2,10]],"date-time":"2021-02-10T17:31:50Z","timestamp":1612978310000},"update-policy":"https:\/\/doi.org\/10.3389\/crossmark-policy","source":"Crossref","is-referenced-by-count":9,"title":["Proteome-Wide Analysis of Lysine 2-Hydroxyisobutyrylated Proteins in Fusarium oxysporum"],"prefix":"10.3389","volume":"12","author":[{"given":"Hengwei","family":"Qian","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lulu","family":"Wang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xianliang","family":"Ma","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xingling","family":"Yi","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Baoshan","family":"Wang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wenxing","family":"Liang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1965","published-online":{"date-parts":[[2021,2,10]]},"reference":[{"key":"B1","doi-asserted-by":"publisher","DOI":"10.3390\/ijms20040923","article-title":"Molecular identification and disease management of date palm sudden decline syndrome in the United Arab Emirates.","volume":"20","author":"Alwahshi","year":"2019","journal-title":"Int. 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