{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,7,30]],"date-time":"2025-07-30T16:03:25Z","timestamp":1753891405646,"version":"3.41.2"},"reference-count":49,"publisher":"Frontiers Media SA","license":[{"start":{"date-parts":[[2021,4,12]],"date-time":"2021-04-12T00:00:00Z","timestamp":1618185600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100012166","name":"National Key Research and Development Program of China","doi-asserted-by":"publisher","award":["2017YFD0500800"],"award-info":[{"award-number":["2017YFD0500800"]}],"id":[{"id":"10.13039\/501100012166","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100012453","name":"China Agricultural Research System","doi-asserted-by":"publisher","award":["CARS-42-17"],"award-info":[{"award-number":["CARS-42-17"]}],"id":[{"id":"10.13039\/501100012453","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100012438","name":"Sichuan Veterinary Medicine and Drug Innovation Group of China Agricultural Research System","doi-asserted-by":"publisher","award":["SCCXTD-2020-18"],"award-info":[{"award-number":["SCCXTD-2020-18"]}],"id":[{"id":"10.13039\/501100012438","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["frontiersin.org"],"crossmark-restriction":true},"short-container-title":["Front. Microbiol."],"abstract":"<jats:p>Duck hepatitis A virus type 1 (DHAV-1) is one of the most deadly pathogens that endanger the duck industry. Most viruses usually turn off host translation after infection to facilitate viral replication and translation. For the first time report to our knowledge, DHAV-1 can induce eIF2\u03b1 phosphorylation and inhibit cellular translation in duck embryo fibroblasts (DEFs). Moreover, the activity of DHAV-1 in the cells caused obvious eIF2\u03b1 phosphorylation, which has nothing to do with the viral protein. Subsequently, we screened two kinases (PERK and GCN2) that affect eIF2\u03b1 phosphorylation through inhibitors and shRNA. Notably, the role of GCN2 in other picornaviruses has not been reported. In addition, when the phosphorylation of eIF2\u03b1 induced by DHAV-1 is inhibited, the translation efficiency of DEFs restores to a normal level, indicating that DHAV-1 induced cellular translation shutoff is dependent on eIF2\u03b1 phosphorylation.<\/jats:p>","DOI":"10.3389\/fmicb.2021.624540","type":"journal-article","created":{"date-parts":[[2021,4,12]],"date-time":"2021-04-12T05:17:52Z","timestamp":1618204672000},"update-policy":"https:\/\/doi.org\/10.3389\/crossmark-policy","source":"Crossref","is-referenced-by-count":6,"title":["Duck Hepatitis A Virus Type 1 Induces eIF2\u03b1 Phosphorylation-Dependent Cellular Translation Shutoff via PERK\/GCN2"],"prefix":"10.3389","volume":"12","author":[{"given":"Yuanzhi","family":"Liu","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Anchun","family":"Cheng","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mingshu","family":"Wang","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sai","family":"Mao","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xumin","family":"Ou","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Qiao","family":"Yang","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ying","family":"Wu","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Qun","family":"Gao","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mafeng","family":"Liu","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Shaqiu","family":"Zhang","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Juan","family":"Huang","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Renyong","family":"Jia","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Dekang","family":"Zhu","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Shun","family":"Chen","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xinxin","family":"Zhao","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yanling","family":"Yu","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yunya","family":"Liu","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ling","family":"Zhang","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Bin","family":"Tian","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Leichang","family":"Pan","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1965","published-online":{"date-parts":[[2021,4,12]]},"reference":[{"key":"ref1","doi-asserted-by":"publisher","first-page":"7683","DOI":"10.1093\/nar\/gkt563","article-title":"The eIF2\u03b1\/ATF4 pathway is essential for stress-induced autophagy gene expression","volume":"41","author":"B\u2019Chir","year":"2013","journal-title":"Nucleic Acids Res."},{"key":"ref2","doi-asserted-by":"publisher","first-page":"1730","DOI":"10.1038\/sj.emboj.7601073","article-title":"Antiviral effect of the mammalian translation initiation factor 2alpha kinase GCN2 against RNA viruses","volume":"25","author":"Berlanga","year":"2006","journal-title":"EMBO J."},{"key":"ref3","doi-asserted-by":"publisher","first-page":"e1008250","DOI":"10.1371\/journal.ppat.1008250","article-title":"Norovirus infection results in eIF2\u03b1 independent host translation shut-off and remodels the G3BP1 interactome evading stress granule formation","volume":"16","author":"Brocard","year":"2020","journal-title":"PLoS Pathog."},{"key":"ref4","doi-asserted-by":"publisher","first-page":"780","DOI":"10.1007\/s11262-016-1364-4","article-title":"The 2A2 protein of duck hepatitis a virus type 1 induces apoptosis in primary cell culture","volume":"52","author":"Cao","year":"2016","journal-title":"Virus Genes"},{"key":"ref5","doi-asserted-by":"publisher","first-page":"284","DOI":"10.3389\/fcimb.2017.00284","article-title":"dsRNA binding domain of PKR is proteolytically released by enterovirus A71 to facilitate viral replication","volume":"7","author":"Chang","year":"2017","journal-title":"Front. 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