{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,7,30]],"date-time":"2025-07-30T16:01:50Z","timestamp":1753891310149,"version":"3.41.2"},"reference-count":46,"publisher":"Frontiers Media SA","license":[{"start":{"date-parts":[[2021,1,28]],"date-time":"2021-01-28T00:00:00Z","timestamp":1611792000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100012166","name":"National Key Research and Development Program of China","doi-asserted-by":"publisher","award":["2018YFD0500900"],"award-info":[{"award-number":["2018YFD0500900"]}],"id":[{"id":"10.13039\/501100012166","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["frontiersin.org"],"crossmark-restriction":true},"short-container-title":["Front. Microbiol."],"abstract":"<jats:p>BAX inhibitor 1 (BI-1) is an evolutionarily conserved transmembrane protein first identified in a screening process for human proteins that suppress BAX-induced apoptosis in yeast cells. Eukaryotic BI-1 is a cytoprotective protein that suppresses cell death induced by multiple stimuli in eukaryotes. <jats:italic>Brucella<\/jats:italic>, the causative agent of brucellosis that threatens public health and animal husbandry, contains a conserved gene that encodes BI-1-like protein. To explore the role of the <jats:italic>Brucella<\/jats:italic> homolog of BI-1, BrBI, in <jats:italic>Brucella suis<\/jats:italic> S2, we constructed the <jats:italic>brbI<\/jats:italic> deletion mutant strain and its complemented strain. <jats:italic>brbI<\/jats:italic> deletion altered the membrane properties of <jats:italic>Brucella suis<\/jats:italic> S2 and decreased its resistance to acidic pH, H<jats:sub>2<\/jats:sub>O<jats:sub>2<\/jats:sub>, polymyxin B, and lincomycin. Additionally, deleting <jats:italic>brbI<\/jats:italic> led to defective growth, cell division, and viability in <jats:italic>Brucella suis<\/jats:italic> S2. We then revealed the effect of <jats:italic>brbI<\/jats:italic> deletion on the physiological characteristics of <jats:italic>Brucella suis<\/jats:italic> S2 via integrated transcriptomic and proteomic analyses. The integrated analysis showed that <jats:italic>brbI<\/jats:italic> deletion significantly affected the expression of multiple genes at the mRNA and\/or protein levels. Specifically, the affected divisome proteins, FtsB, FtsI, FtsL, and FtsQ, may be the molecular basis of the impaired cell division of the <jats:italic>brbI<\/jats:italic> mutant strain, and the extensively affected membrane proteins and transporter-associated proteins were consistent with the phenotype of the membrane properties\u2019 alterations of the <jats:italic>brbI<\/jats:italic> mutant strain. In conclusion, our results revealed that BrBI is a bacterial cytoprotective protein involved in membrane homeostasis, cell division, and stress resistance in <jats:italic>Brucella suis<\/jats:italic> S2.<\/jats:p>","DOI":"10.3389\/fmicb.2021.632095","type":"journal-article","created":{"date-parts":[[2021,1,28]],"date-time":"2021-01-28T09:04:10Z","timestamp":1611824650000},"update-policy":"https:\/\/doi.org\/10.3389\/crossmark-policy","source":"Crossref","is-referenced-by-count":8,"title":["Integrated Proteomic and Transcriptomic Analyses Reveal the Roles of Brucella Homolog of BAX Inhibitor 1 in Cell Division and Membrane Homeostasis of Brucella suis S2"],"prefix":"10.3389","volume":"12","author":[{"given":"Guangdong","family":"Zhang","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Fangli","family":"Zhong","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Lei","family":"Chen","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Peipei","family":"Qin","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Junmei","family":"Li","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Feijie","family":"Zhi","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Lulu","family":"Tian","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Dong","family":"Zhou","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Pengfei","family":"Lin","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Huatao","family":"Chen","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Keqiong","family":"Tang","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Wei","family":"Liu","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yaping","family":"Jin","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Aihua","family":"Wang","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1965","published-online":{"date-parts":[[2021,1,28]]},"reference":[{"key":"B1","doi-asserted-by":"publisher","first-page":"713","DOI":"10.1128\/IAI.00713-17","article-title":"Brucella abortus Senses the Intracellular Environment through the BvrR\/BvrS Two-Component System, Which Allows B. abortus To Adapt to Its Replicative Niche.","volume":"86","author":"Altamirano-Silva","year":"2018","journal-title":"Infect. 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