{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,23]],"date-time":"2026-08-23T16:06:58Z","timestamp":1787501218040,"version":"build-2736575974"},"reference-count":67,"publisher":"Frontiers Media SA","license":[{"start":{"date-parts":[[2021,3,18]],"date-time":"2021-03-18T00:00:00Z","timestamp":1616025600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["frontiersin.org"],"crossmark-restriction":true},"short-container-title":["Front. Microbiol."],"abstract":"<jats:p>\n                    Lysostaphin is an effective antimicrobial agent to\n                    <jats:italic>Staphylococcus<\/jats:italic>\n                    , especially for the methicillin-resistant\n                    <jats:italic>Staphylococcus aureus<\/jats:italic>\n                    (MRSA) and multidrug-resistant\n                    <jats:italic>Staphylococcus aureus<\/jats:italic>\n                    (MDRSA). In this study, the seven lysostaphin derived mutants (rLys) were designed to overcome the barrier of glycosylation during expression in\n                    <jats:italic>Pichia pastoris<\/jats:italic>\n                    . Among them, 127A and 127A232Q had highest antimicrobial activity (MIC values 0.07\u20130.3 \u03bcM) to\n                    <jats:italic>S. aureus<\/jats:italic>\n                    than others and the commercial lysostaphins (1\u201315.8 times). There was no glycosylation during the expression in 5-L fermenter level, with the high yield of 1315 mg\/L (127A) and 1141 mg\/L (127A232Q), respectively. Meanwhile, 127A and 127A232Q effectively killed 99.9% of\n                    <jats:italic>S. aureus<\/jats:italic>\n                    at low concentration (1 \u00d7 MIC) within 30 min, without the regrowth of pathogen. They also showed low toxicity, high pH and temperature stability. The results of\n                    <jats:italic>in vivo<\/jats:italic>\n                    therapeutic effect of 127A and 127A232Q against high virulent\n                    <jats:italic>S. aureus<\/jats:italic>\n                    CVCC546 showed that 127A and 127A232Q increased the survival rate of infected mice up to 100% at the dose of 10 mg\/kg than the untreated group, reduced the bacterial translocation by 5-7 log CFU (over 99%) in organs compared to the untreated group and alleviated multiple-organ injuries (liver, kidney and spleen). These data indicated that the non-glycosylated lysostaphin 127A and 127A232Q may be a promising therapeutic agent against MDR staphylococcal infections.\n                  <\/jats:p>","DOI":"10.3389\/fmicb.2021.637662","type":"journal-article","created":{"date-parts":[[2021,3,18]],"date-time":"2021-03-18T03:51:41Z","timestamp":1616039501000},"update-policy":"https:\/\/doi.org\/10.3389\/crossmark-policy","source":"Crossref","is-referenced-by-count":18,"title":["Design and High Expression of Non-glycosylated Lysostaphins in Pichia pastoris and Their Pharmacodynamic Study"],"prefix":"10.3389","volume":"12","author":[{"given":"Wenluan","family":"Shen","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Na","family":"Yang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Da","family":"Teng","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ya","family":"Hao","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xuanxuan","family":"Ma","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ruoyu","family":"Mao","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jianhua","family":"Wang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1965","published-online":{"date-parts":[[2021,3,18]]},"reference":[{"key":"B1","doi-asserted-by":"publisher","first-page":"4789","DOI":"10.1002\/j.1460-2075.1996.tb00859.x","article-title":"Target cell specificity of a bacteriocin molecule: a C-terminal signal directs lysostaphin to the cell wall of Staphylococcus aureus.","volume":"15","author":"Baba","year":"1996","journal-title":"EMBO J."},{"key":"B2","doi-asserted-by":"publisher","first-page":"1384","DOI":"10.1590\/S0103-50532010000700026","article-title":"An inorganic complex that inhibits Mycobacterium tuberculosis enoyl reductase as a prototype of a new class of chemotherapeutic agents to treat tuberculosis.","volume":"21","author":"Basso","year":"2010","journal-title":"J. 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