{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,15]],"date-time":"2026-10-15T01:23:16Z","timestamp":1792027396830,"version":"4.4.0"},"reference-count":70,"publisher":"Frontiers Media SA","license":[{"start":{"date-parts":[[2021,2,4]],"date-time":"2021-02-04T00:00:00Z","timestamp":1612396800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100013142","name":"Key Research and Development Project of Hainan Province","doi-asserted-by":"publisher","award":["ZDYF2020088"],"award-info":[{"award-number":["ZDYF2020088"]}],"id":[{"id":"10.13039\/501100013142","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["31660744"],"award-info":[{"award-number":["31660744"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["frontiersin.org"],"crossmark-restriction":true},"short-container-title":["Front. Microbiol."],"abstract":"<jats:p>Protease-producing bacteria play vital roles in degrading organic matter of aquaculture system, while the knowledge of diversity and bacterial community structure of protease-producing bacteria is limited in this system, especially in the tropical region. Herein, 1,179 cultivable protease-producing bacterial strains that belonged to Actinobacteria, Firmicutes, and Proteobacteria were isolated from tropical aquaculture systems, of which the most abundant genus was <jats:italic>Bacillus<\/jats:italic>, followed by <jats:italic>Vibrio<\/jats:italic>. The diversity and relative abundance of protease-producing bacteria in sediment were generally higher than those in water. Twenty-one genera from sediment and 16 genera from water were identified, of which <jats:italic>Bacillus<\/jats:italic> dominated by <jats:italic>Bacillus hwajinpoensis<\/jats:italic> in both and <jats:italic>Vibrio<\/jats:italic> dominated by <jats:italic>Vibrio owensii<\/jats:italic> in water were the dominant genera. The unique genera in sediment or water accounted for tiny percentage may play important roles in the stability of community structure. Eighty <jats:italic>V. owensii<\/jats:italic> isolates were clustered into four clusters (ET-1\u2013ET-4) at 58% of similarity by ERIC-PCR (enterobacterial repetitive intergenic consensus-polymerase chain reaction), which was identified as a novel branch of <jats:italic>V. owensii<\/jats:italic>. Additionally, <jats:italic>V. owensii<\/jats:italic> strains belonged to ET-3 and ET-4 were detected in most aquaculture ponds without outbreak of epidemics, indicating that these protease-producing bacteria may be used as potential beneficial bacteria for wastewater purification. Environmental variables played important roles in shaping protease-producing bacterial diversity and community structure in aquaculture systems. In sediment, dissolved oxygen (DO), chemical oxygen demand (COD), and salinity as the main factors positively affected the distributions of dominant genus (<jats:italic>Vibrio<\/jats:italic>) and unique genera (<jats:italic>Planococcus<\/jats:italic> and <jats:italic>Psychrobacter<\/jats:italic>), whereas temperature negatively affected that of <jats:italic>Bacillus<\/jats:italic> (except <jats:italic>B. hwajinpoensis<\/jats:italic>). In water, <jats:italic>Alteromonas<\/jats:italic> as unique genus and <jats:italic>Photobacterium<\/jats:italic> were negatively affected by NO<jats:sub>3<\/jats:sub><jats:sup>\u2212<\/jats:sup>-N and NO<jats:sub>2<\/jats:sub><jats:sup>\u2212<\/jats:sup>-N, respectively, whereas pH as the main factor positively affected the distribution of <jats:italic>Photobacterium<\/jats:italic>. These findings will lay a foundation for the development of protease-producing bacterial agents for wastewater purification and the construction of an environment-friendly tropical aquaculture model.<\/jats:p>","DOI":"10.3389\/fmicb.2021.638129","type":"journal-article","created":{"date-parts":[[2021,2,4]],"date-time":"2021-02-04T05:35:58Z","timestamp":1612416958000},"update-policy":"https:\/\/doi.org\/10.3389\/crossmark-policy","source":"Crossref","is-referenced-by-count":30,"title":["Community Structure of Protease-Producing Bacteria Cultivated From Aquaculture Systems: Potential Impact of a Tropical Environment"],"prefix":"10.3389","volume":"12","author":[{"given":"Yali","family":"Wei","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jun","family":"Bu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hao","family":"Long","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiang","family":"Zhang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiaoni","family":"Cai","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Aiyou","family":"Huang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wei","family":"Ren","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhenyu","family":"Xie","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1965","published-online":{"date-parts":[[2021,2,4]]},"reference":[{"key":"ref1","doi-asserted-by":"publisher","first-page":"2016","DOI":"10.1111\/j.1365-2672.2007.03445.x","article-title":"Diversity of tetracycline resistance genes in bacteria from aquaculture sources in Australia","volume":"103","author":"Akinbowale","year":"2010","journal-title":"J. Appl. Microbiol."},{"key":"ref2","doi-asserted-by":"publisher","first-page":"467","DOI":"10.1111\/jfd.12257","article-title":"Isolation and screening of probiotic candidates from marron, C herax cainii (Austin, 2002) gastrointestinal tract (GIT) and commercial probiotic products for the use in marron culture","volume":"38","author":"Ambas","year":"2015","journal-title":"J. Fish Dis."},{"key":"ref3","doi-asserted-by":"publisher","first-page":"30","DOI":"10.1016\/j.aqrep.2018.09.004","article-title":"Marine protease-producing bacterium and its potential use as an abalone probiont","volume":"12","author":"Amin","year":"2018","journal-title":"Aquacult. Rep."},{"key":"ref4","doi-asserted-by":"publisher","first-page":"1","DOI":"10.4265\/bio.25.1","article-title":"Postbiotics applications as infectious disease control agent in aquaculture","volume":"25","author":"Ang","year":"2020","journal-title":"Biocontrol Sci."},{"key":"ref5","doi-asserted-by":"publisher","first-page":"488","DOI":"10.1111\/j.1365-2095.2010.00826.x","article-title":"The effect of lactic acid bacteria administration on growth, digestive enzyme activity and gut microbiota in Persian sturgeon (Acipenser persicus) and beluga (Huso huso) fry","volume":"17","author":"Askarian","year":"2011","journal-title":"Aquac. Nutr."},{"key":"ref6","doi-asserted-by":"publisher","first-page":"529","DOI":"10.1016\/j.bjm.2017.01.014","article-title":"Enterobacterial repetitive intergenic consensus (ERIC)-PCR analysis as a reliable evidence for suspected Shigella spp. outbreaks","volume":"49","author":"Bakhshi","year":"2018","journal-title":"Braz. J. Microbiol."},{"key":"ref7","doi-asserted-by":"publisher","first-page":"30","DOI":"10.1016\/j.aquaeng.2012.11.009","article-title":"Insight into bacterial population in aquaculture systems and its implication","volume":"53","author":"Blancheton","year":"2013","journal-title":"Aquac. Eng."},{"key":"ref8","doi-asserted-by":"publisher","first-page":"175","DOI":"10.1111\/j.1574-6968.2009.01850.x","article-title":"Vibrio owensii sp. nov., isolated from cultured crustaceans in Australia","volume":"302","author":"Cano-Gomez","year":"2010","journal-title":"FEMS Microbiol. Lett."},{"key":"ref9","doi-asserted-by":"publisher","first-page":"402","DOI":"10.1016\/j.ijfoodmicro.2011.12.001","article-title":"Molecular typing of Vibrio parahaemolyticus isolates from the middle-east coastline of China","volume":"153","author":"Chen","year":"2012","journal-title":"Int. J. Food Microbiol."},{"key":"ref10","doi-asserted-by":"publisher","first-page":"2681","DOI":"10.13227\/j.hjkx.2016.07.035","article-title":"Screening and nitrogen removing characteristics of heterotrophic nitrification-aerobic denitrification bacteria SLWX2 from sea water","volume":"37","author":"Cheng","year":"2016","journal-title":"Huanjing Kexue."},{"key":"ref11","doi-asserted-by":"publisher","first-page":"012002","DOI":"10.1088\/1755-1315\/266\/1\/012002","article-title":"Isolation and screening of cellulose and organic matter degrading bacteria from aquaculture ponds for improving water quality in aquaculture","volume":"266","author":"Dat","year":"2019","journal-title":"IOP Conf. Se.: Earth and Environ. Sci."},{"key":"ref12","doi-asserted-by":"publisher","first-page":"7774","DOI":"10.1073\/pnas.0803070105","article-title":"A latitudinal diversity gradient in planktonic marine bacteria","volume":"105","author":"Fuhrman","year":"2008","journal-title":"Proc. Natl. Acad. Sci. U. S. A."},{"key":"ref13","doi-asserted-by":"publisher","first-page":"607","DOI":"10.1016\/j.aquaculture.2018.10.032","article-title":"Poly-beta-hydroxybutyrate (PHB)-accumulating Halomonas improves the survival, growth, robustness and modifies the gut microbial composition of Litopenaeus vannamei postlarvae","volume":"500","author":"Gao","year":"2019","journal-title":"Aquaculture"},{"key":"ref14","doi-asserted-by":"publisher","first-page":"289","DOI":"10.1111\/j.1365-2095.2007.00529.x","article-title":"Dietary probiotic supplementation in growth and health of live-bearing ornamental fishes","volume":"14","author":"Ghosh","year":"2008","journal-title":"Aquac. Nutr."},{"key":"ref15","doi-asserted-by":"publisher","first-page":"1","DOI":"10.1016\/j.fsi.2016.06.059","article-title":"Analysis of digital gene expression profiling in hemocytes of white shrimp Litopenaeus vannamei under nitrite stress","volume":"56","author":"Guo","year":"2016","journal-title":"Fish Shellfish Immunol."},{"key":"ref16","doi-asserted-by":"publisher","first-page":"917","DOI":"10.1111\/jam.12886","article-title":"The use of probiotics in aquaculture","volume":"119","author":"Hai","year":"2015","journal-title":"J. Appl. Microbiol."},{"key":"ref17","doi-asserted-by":"publisher","first-page":"fix140","DOI":"10.1093\/femsec\/fix140","article-title":"Biosurfactant from a marine bacterium disrupts biofilms of pathogenic bacteria in a tropical aquaculture system","volume":"93","author":"Hamza","year":"2017","journal-title":"FEMS Microbiol. Ecol."},{"key":"ref18","doi-asserted-by":"publisher","first-page":"2894","DOI":"10.1016\/j.scitotenv.2011.04.030","article-title":"Antibiotic contamination and occurrence of antibiotic-resistant bacteria in aquatic environments of northern Vietnam","volume":"409","author":"Hoa","year":"2011","journal-title":"Sci. Total Environ."},{"key":"ref19","doi-asserted-by":"publisher","first-page":"285","DOI":"10.1111\/j.1574-6941.1999.tb00656.x","article-title":"Marine Pseudoalteromonas species are associated with higher organisms and produce biologically active extracellular agents","volume":"30","author":"Holmstr\u00f6m","year":"1999","journal-title":"FEMS Microbiol. Ecol."},{"key":"ref20","doi-asserted-by":"publisher","first-page":"2432","DOI":"10.1111\/are.14587","article-title":"Comparison of bacterial communities in channel catfish Ictalurus punctatus culture ponds of an industrial ecological purification recirculating aquaculture system","volume":"51","author":"Hucheng","year":"2020","journal-title":"Aquac. Res."},{"key":"ref21","doi-asserted-by":"publisher","first-page":"1285","DOI":"10.1007\/s12562-009-0138-5","article-title":"Colonization and probiotic effects of lactic acid bacteria in the gut of the abalone Haliotis gigantea","volume":"75","author":"Iehata","year":"2009","journal-title":"Fish. Sci."},{"key":"ref22","doi-asserted-by":"publisher","first-page":"321","DOI":"10.1016\/j.vetmic.2006.06.012","article-title":"Virulence and enterobacterial repetitive intergenic consensus PCR of Vibrio alginolyticus strains isolated from Tunisian cultured gilthead sea bream and sea bass outbreaks","volume":"117","author":"Kahla-Nakbi","year":"2006","journal-title":"Vet. Microbiol."},{"key":"ref23","doi-asserted-by":"publisher","first-page":"49","DOI":"10.1016\/j.aquaculture.2010.09.019","article-title":"Alteromonas macleodii 0444 and Neptunomonas sp. 0536, two novel probiotics for hatchery-reared Greenshell \u2122 mussel larvae, Perna canaliculus","volume":"309","author":"Kesarcodi-Watson","year":"2010","journal-title":"Aquaculture"},{"key":"ref24","doi-asserted-by":"publisher","first-page":"114939","DOI":"10.1016\/j.envpol.2020.114939","article-title":"Transcriptomics of Planococcus kocurii O516 reveals the degrading metabolism of sulfamethoxazole in marine aquaculture wastewater","volume":"265","author":"Kong","year":"2020","journal-title":"Environ. Pollut."},{"key":"ref25","doi-asserted-by":"publisher","first-page":"820","DOI":"10.1016\/j.fsi.2019.02.010","article-title":"A review on the application of Bacillus as probiotics in aquaculture","volume":"87","author":"Kuebutornye","year":"2019","journal-title":"Fish Shellfish Immunol."},{"key":"ref26","doi-asserted-by":"publisher","first-page":"109","DOI":"10.1016\/j.aquaculture.2010.09.014","article-title":"Chronic toxicity of nitrate to pacific white shrimp, Litopenaeus vannamei: impacts on survival, growth, antennae length, and pathology","volume":"309","author":"Kuhn","year":"2010","journal-title":"Aquaculture"},{"key":"ref27","doi-asserted-by":"publisher","first-page":"204","DOI":"10.1016\/j.jenvman.2016.08.017","article-title":"Genotoxicity evaluation of tannery effluent treated with newly isolated hexavalent chromium reducing Bacillus cereus","volume":"183","author":"Kumari","year":"2016","journal-title":"J. Environ. Manag."},{"key":"ref28","doi-asserted-by":"publisher","first-page":"37","DOI":"10.1111\/jam.14437","article-title":"Virulence properties of three new Photobacterium species affecting cultured fish","volume":"129","author":"Labella","year":"2020","journal-title":"J. Appl. Microbiol."},{"key":"ref29","doi-asserted-by":"publisher","first-page":"797","DOI":"10.3389\/fmicb.2018.00797","article-title":"Bacterial diversity and nitrogen utilization strategies in the upper layer of the northwestern Pacific Ocean","volume":"9","author":"Li","year":"2018","journal-title":"Front. Microbiol."},{"key":"ref30","doi-asserted-by":"publisher","first-page":"140883","DOI":"10.1016\/j.scitotenv.2020.140883","article-title":"Effectiveness of dredging on internal phosphorus loading in a typical aquacultural lake","volume":"744","author":"Li","year":"2020","journal-title":"Sci. Total Environ."},{"key":"ref31","doi-asserted-by":"publisher","first-page":"109","DOI":"10.1016\/S0022-0981(01)00227-1","article-title":"Acute toxicity of ammonia on Litopenaeus vannamei Boone juveniles at different salinity levels","volume":"259","author":"Lin","year":"2001","journal-title":"J. Exp. Mar. Biol. Ecol."},{"key":"ref32","doi-asserted-by":"publisher","first-page":"193","DOI":"10.1016\/S0044-8486(03)00220-5","article-title":"Acute toxicity of nitrite on Litopenaeus vannamei (Boone) juveniles at different salinity levels","volume":"224","author":"Lin","year":"2003","journal-title":"Aquaculture"},{"key":"ref33","doi-asserted-by":"publisher","first-page":"9","DOI":"10.1016\/j.biortech.2016.01.066","article-title":"Marinobacter strain NNA5, a newly isolated and highly efficient aerobic denitrifier with zero N2O emission","volume":"206","author":"Liu","year":"2016","journal-title":"Bioresour. Technol."},{"key":"ref34","doi-asserted-by":"publisher","first-page":"156","DOI":"10.1016\/j.jip.2018.02.005","article-title":"A Vibrio owensii strain as the causative agent of AHPND in cultured shrimp, Litopenaeus vannamei","volume":"153","author":"Liu","year":"2018","journal-title":"J. Invertebr. Pathol."},{"key":"ref35","doi-asserted-by":"publisher","first-page":"194","DOI":"10.1016\/j.fsi.2017.01.001","article-title":"Evaluation of marine psychrophile, Psychrobacter namhaensis SO89, as a probiotic in Nile tilapia (Oreochromis niloticus) diets","volume":"61","author":"Makled","year":"2017","journal-title":"Fish Shellfish Immunol."},{"key":"ref36","doi-asserted-by":"publisher","first-page":"734516","DOI":"10.1016\/j.aquaculture.2019.734516","article-title":"Effect of aeration intensity on the biofilm nitrification process during the production of the white shrimp Litopenaeus vannamei (Boone, 1931) in Biofloc and clear water systems","volume":"514","author":"Mariane De Morais","year":"2020","journal-title":"Aquaculture"},{"key":"ref37","doi-asserted-by":"publisher","first-page":"2372","DOI":"10.4238\/vol9-4gmr921","article-title":"Diversity of bacterial endophytes in roots of Mexican husk tomato plants (Physalis ixocarpa) and their detection in the rhizosphere","volume":"9","author":"Marquez-Santacruz","year":"2010","journal-title":"Genet. Mol. Res."},{"key":"ref38","doi-asserted-by":"publisher","first-page":"853","DOI":"10.1007\/s00203-013-0937-z","article-title":"Diazotrophic diversity in the Caribbean coral, Montastraea cavernosa","volume":"195","author":"Olson","year":"2013","journal-title":"Arch. Microbiol."},{"key":"ref39","doi-asserted-by":"publisher","first-page":"170","DOI":"10.1006\/geno.2001.6469","article-title":"Characterization of exopolysaccharides produced by Bacillus cereus and Brachybacterium sp. isolated from Asian sea bass (Lates calcarifer). Mal","volume":"8","author":"Orsod","year":"2012","journal-title":"J. Microbiol."},{"key":"ref40","doi-asserted-by":"publisher","first-page":"100637","DOI":"10.1016\/j.genrep.2020.100637","article-title":"Molecular analysis of Proteus mirabilis isolated from urine samples using ERIC-PCR","volume":"19","author":"Pishbin","year":"2020","journal-title":"Gene Reports."},{"key":"ref41","doi-asserted-by":"publisher","first-page":"187","DOI":"10.3354\/ame01431","article-title":"Temperature and substrates as interactive limiting factors for marine heterotrophic bacteria","volume":"23","author":"Pomeroy","year":"2001","journal-title":"Aquat. Microb. Ecol."},{"key":"ref42","doi-asserted-by":"publisher","first-page":"271","DOI":"10.3389\/fmicb.2017.00271","article-title":"Fine flounder (Paralichthys adspersus) microbiome showed important differences between wild and reared specimens","volume":"8","author":"Ramirez","year":"2017","journal-title":"Front. Microbiol."},{"key":"ref43","doi-asserted-by":"publisher","first-page":"1844","DOI":"10.3389\/fmicb.2017.01844","article-title":"The microbiome of Seriola lalandi of wild and aquaculture origin reveals differences in composition and potential function","volume":"8","author":"Ram\u00edrez","year":"2017","journal-title":"Front. Microbiol."},{"key":"ref44","doi-asserted-by":"publisher","first-page":"203","DOI":"10.1007\/s10499-014-9809-z","article-title":"Evaluation of Bacillus amyloliquefaciens on the growth performance, intestinal morphology, hematology and body composition of Nile tilapia, Oreochromis niloticus","volume":"23","author":"Reda","year":"2015","journal-title":"Aquac. Int."},{"key":"ref45","doi-asserted-by":"publisher","first-page":"51","DOI":"10.3390\/md16020051","article-title":"Purification and characterization of a biofilm-degradable dextranase from a marine bacterium","volume":"16","author":"Ren","year":"2018","journal-title":"Mar. Drugs"},{"key":"ref46","doi-asserted-by":"publisher","first-page":"4355","DOI":"10.1021\/acs.jafc.9b01290","article-title":"Crystal structure of GH49 dextranase from Arthrobacter oxidans KQ11: identification of catalytic base and improvement of thermostability using semirational design based on B-factors","volume":"67","author":"Ren","year":"2019","journal-title":"J. Agric. Food Chem."},{"key":"ref47","doi-asserted-by":"publisher","first-page":"301","DOI":"10.1016\/S0044-8486(98)00305-6","article-title":"Effects of a probiotic bacterium on black tiger shrimp Penaeus monodon survival and growth","volume":"167","author":"Rengpipat","year":"1998","journal-title":"Aquaculture"},{"key":"ref48","doi-asserted-by":"publisher","first-page":"829","DOI":"10.1016\/j.aquaculture.2006.06.030","article-title":"Characterisation of the microbiota associated with intestine of Atlantic cod (Gadus morhua L.) - the effect of fish meal, standard soybean meal and a bioprocessed soybean meal","volume":"261","author":"Ringo","year":"2006","journal-title":"Aquaculture"},{"key":"ref49","doi-asserted-by":"publisher","first-page":"175","DOI":"10.3354\/dao069175","article-title":"Molecular fingerprinting of Vibrio tapetis strains using three PCR-based methods: ERIC-PCR, REP-PCR and RAPD","volume":"69","author":"Rodr\u00edguez","year":"2006","journal-title":"Dis. Aquat. Org."},{"key":"ref50","doi-asserted-by":"publisher","first-page":"135","DOI":"10.1016\/j.ijantimicag.2018.03.010","article-title":"Antimicrobial resistance in aquaculture: current knowledge and alternatives to tackle the problem","volume":"52","author":"Santos","year":"2018","journal-title":"Int. J. Antimicrob. Agents"},{"key":"ref51","doi-asserted-by":"publisher","first-page":"221","DOI":"10.1016\/j.micpath.2018.01.038","article-title":"Synthesis of silver nanoparticles from Bacillus brevis (NCIM 2533) and their antibacterial activity against pathogenic bacteria","volume":"116","author":"Saravanan","year":"2018","journal-title":"Microb. Pathog."},{"key":"ref52","doi-asserted-by":"publisher","first-page":"496","DOI":"10.1016\/j.fsi.2015.03.004","article-title":"Improvement of immunity and disease resistance in the Nile tilapia, Oreochromis niloticus, by dietary supplementation with Bacillus amyloliquefaciens","volume":"44","author":"Selim","year":"2015","journal-title":"Fish Shellfish Immunol."},{"key":"ref53","doi-asserted-by":"publisher","first-page":"534","DOI":"10.1007\/s12602-017-9290-x","article-title":"Properties of probiotics Kocuria SM1 and Rhodococcus SM2 isolated from fish guts","volume":"10","author":"Sharifuzzaman","year":"2018","journal-title":"Probiotics Antimicrob. Proteins."},{"key":"ref54","doi-asserted-by":"publisher","first-page":"37","DOI":"10.1016\/j.aquaculture.2016.03.049","article-title":"Effects of protease supplementation in low fish meal pelleted and extruded diets on growth, nutrient retention and digestibility of gibel carp, Carassius auratus gibelio","volume":"460","author":"Shi","year":"2016","journal-title":"Aquaculture"},{"key":"ref55","doi-asserted-by":"publisher","first-page":"37","DOI":"10.1016\/j.biori.2018.03.001","article-title":"Isolation, characterization and identification of extracellular enzyme producer Bacillus licheniformis from municipal wastewater and evaluation of their biodegradability","volume":"2","author":"Sonune","year":"2018","journal-title":"Biotechnol. Res. Inno."},{"key":"ref56","doi-asserted-by":"publisher","first-page":"e9055","DOI":"10.7717\/peerj.9055","article-title":"Diversity of cultivable protease-producing bacteria and their extracellular proteases associated to scleractinian corals","volume":"8","author":"Su","year":"2020","journal-title":"PeerJ."},{"key":"ref57","doi-asserted-by":"publisher","first-page":"415","DOI":"10.1007\/s00248-007-9287-1","article-title":"Endophytic bacterial diversity in rice (Oryza sativa L.) roots estimated by 16S rDNA sequence analysis","volume":"55","author":"Sun","year":"2008","journal-title":"Microb. Ecol."},{"key":"ref58","doi-asserted-by":"publisher","first-page":"637","DOI":"10.5897\/AJMR.9000353","article-title":"Gut microbiota of fast and slow growing grouper Epinephelus coioides","volume":"3","author":"Sun","year":"2009","journal-title":"Afr. J. Microbiol. Res."},{"key":"ref59","doi-asserted-by":"publisher","first-page":"140","DOI":"10.1016\/j.aquaculture.2008.04.020","article-title":"Lactobacillus spp. bacteria as probiotics in gilthead sea bream (Sparus aurata, L.) larvae: effects on growth performance and digestive enzyme activities","volume":"280","author":"Suzer","year":"2008","journal-title":"Aquaculture"},{"key":"ref60","doi-asserted-by":"publisher","first-page":"135","DOI":"10.1264\/jsme2.ME10204","article-title":"Fluoroquinolone (FQ) contamination does not correlate with occurrence of FQ-resistant bacteria in aquatic environments of Vietnam and Thailand","volume":"26","author":"Takasu","year":"2011","journal-title":"Microbes Environ."},{"key":"ref61","doi-asserted-by":"publisher","first-page":"2155","DOI":"10.3389\/fmicb.2018.02155","article-title":"Molecular epidemiology of Photobacterium damselae subsp damselae outbreaks in marine rainbow trout farms reveals extensive horizontal gene transfer and high genetic diversity","volume":"9","author":"Terceti","year":"2018","journal-title":"Front. Microbiol."},{"key":"ref62","doi-asserted-by":"publisher","first-page":"646","DOI":"10.3389\/fmicb.2016.00646","article-title":"Selection of the N-Acylhomoserine lactone-degrading bacterium Alteromonas stellipolaris PQQ-42 and of its potential for biocontrol in aquaculture","volume":"7","author":"Torres","year":"2016","journal-title":"Front. Microbiol."},{"key":"ref63","doi-asserted-by":"publisher","first-page":"655","DOI":"10.1023\/A:1024127100993","article-title":"Probiotic bacteria as biological control agents in aquaculture","volume":"64","author":"Verschuere","year":"2000","journal-title":"Microbiol. Mol. Biol. Rev."},{"key":"ref64","doi-asserted-by":"publisher","first-page":"80","DOI":"10.1016\/j.ecoenv.2019.04.066","article-title":"Influence and characteristics of Bacillus stearothermophilus in ammonia reduction during layer manure composting","volume":"180","author":"Wang","year":"2019","journal-title":"Ecotoxicol. Environ. Saf."},{"key":"ref65","doi-asserted-by":"publisher","first-page":"425","DOI":"10.1007\/s00248-015-0583-x","article-title":"Antibiotics, antibiotic resistance genes, and bacterial community composition in fresh water aquaculture environment in China","volume":"70","author":"Wenguang","year":"2015","journal-title":"Microb. Ecol."},{"key":"ref66","doi-asserted-by":"publisher","first-page":"283","DOI":"10.1016\/j.anifeedsci.2005.09.003","article-title":"Effect of probiotics for common carp (Cyprinus carpio) based on growth performance and digestive enzyme activities","volume":"127","author":"Yanbo","year":"2006","journal-title":"Anim. Feed Sci. Technol."},{"key":"ref67","doi-asserted-by":"publisher","first-page":"001","DOI":"10.4172\/2155-9546.S1-001","article-title":"Probiotic Psychrobacter sp. improved the autochthonous microbial diversity along the gastrointestinal tract of grouper Epinephelus coioides","author":"Yang","year":"2011","journal-title":"J. Aquac. Res. Dev."},{"key":"ref68","doi-asserted-by":"publisher","first-page":"322","DOI":"10.1016\/j.fsi.2018.04.055","article-title":"Probiotic potential of Bacillus velezensis JW: antimicrobial activity against fish pathogenic bacteria and immune enhancement effects on Carassius auratus","volume":"78","author":"Yi","year":"2018","journal-title":"Fish Shellfish Immunol."},{"key":"ref69","doi-asserted-by":"publisher","first-page":"1297","DOI":"10.1016\/S0370-2693(97)01497-4","article-title":"Bacillus marisflavi sp. nov. and Bacillus aquimaris sp. nov., isolated from sea water of a tidal flat of the Yellow Sea in Korea","volume":"53","author":"Yoon","year":"2003","journal-title":"J. Med. Microbiol."},{"key":"ref70","doi-asserted-by":"publisher","first-page":"112","DOI":"10.1111\/1574-6968.12277","article-title":"Isolation and characterization of five lytic bacteriophages infecting a Vibrio strain closely related to Vibrio owensii","volume":"348","author":"Yu","year":"2013","journal-title":"FEMS Microbiol. 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