{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,1]],"date-time":"2026-09-01T17:36:57Z","timestamp":1788284217195,"version":"build-2803163510"},"reference-count":68,"publisher":"Frontiers Media SA","license":[{"start":{"date-parts":[[2021,4,9]],"date-time":"2021-04-09T00:00:00Z","timestamp":1617926400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001807","name":"Funda\u00e7\u00e3o de Amparo \u00e0 Pesquisa do Estado de S\u00e3o Paulo","doi-asserted-by":"publisher","award":["2018\/19539-0"],"award-info":[{"award-number":["2018\/19539-0"]}],"id":[{"id":"10.13039\/501100001807","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["frontiersin.org"],"crossmark-restriction":true},"short-container-title":["Front. Microbiol."],"abstract":"<jats:p>The pandemic <jats:italic>Escherichia coli<\/jats:italic> sequence type 131 (ST131) carrying plasmid-mediated colistin resistance <jats:italic>mcr<\/jats:italic> genes has emerged worldwide causing extraintestinal infections, with lineages belonging to three major clades (A, B, and C). Clade B is the most prevalent in animals, contaminating associated meat products, and can be transmitted zoonotically. However, the <jats:italic>bla<\/jats:italic><jats:sub>CTX\u2013M\u201315<\/jats:sub> gene has only been associated with C2 subclade so far. In this study, we performed a genomic investigation of an <jats:italic>E. coli<\/jats:italic> (strain S802) isolated from a kale crop in Brazil, which exhibited a multidrug-resistant (MDR) profile to clinically significant antimicrobials (i.e., polymyxin, broad-spectrum cephalosporins, aminoglycosides, and fluoroquinolones). Whole-genome sequencing analysis revealed that the S802 strain belonged to serotype O25:H4, ST131\/CC131, phylogenetic group B2, and virotype D5. Furthermore, S802 carried the clade B-associated <jats:italic>fimH22<\/jats:italic> allele, genes encoding resistance to clinically important antimicrobials, metals, and biocides, and was phylogenetically related to human, avian, and swine ST131-<jats:italic>H<\/jats:italic>22 strains. Additionally, IncHI2-IncQ1, IncF [F2:A-:B1], and ColE1-like plasmids were identified harboring <jats:italic>mcr-1.1<\/jats:italic>, <jats:italic>bla<\/jats:italic><jats:sub>CTX\u2013M\u201315<\/jats:sub>, and <jats:italic>qnrB19<\/jats:italic>, respectively. The emergence of the <jats:italic>E. coli<\/jats:italic> ST131-<jats:italic>H<\/jats:italic>22 sublineage carrying <jats:italic>mcr-1.1<\/jats:italic>, <jats:italic>bla<\/jats:italic><jats:sub>CTX\u2013M\u201315<\/jats:sub>, and <jats:italic>qnrB19<\/jats:italic> in agricultural soil represents a threat to food and environmental safety. Therefore, a One Health approach to genomic surveillance studies is required to effectively detect and limit the spread of antimicrobial-resistant bacteria and their resistance genes.<\/jats:p>","DOI":"10.3389\/fmicb.2021.659900","type":"journal-article","created":{"date-parts":[[2021,4,9]],"date-time":"2021-04-09T04:31:57Z","timestamp":1617942717000},"update-policy":"https:\/\/doi.org\/10.3389\/crossmark-policy","source":"Crossref","is-referenced-by-count":32,"title":["Colistin-Resistant mcr-1-Positive Escherichia coli ST131-H22 Carrying blaCTX\u2013M\u201315 and qnrB19 in Agricultural Soil"],"prefix":"10.3389","volume":"12","author":[{"given":"Ralf","family":"Lopes","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jo\u00e3o Pedro Rueda","family":"Furlan","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lucas David Rodrigues","family":"dos Santos","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Inara Fernanda Lage","family":"Gallo","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Eliana Guedes","family":"Stehling","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1965","published-online":{"date-parts":[[2021,4,9]]},"reference":[{"key":"B1","doi-asserted-by":"publisher","first-page":"3380","DOI":"10.1093\/bioinformatics\/btw493","article-title":"plasmidSPAdes: assembling plasmids from whole genome sequencing data.","volume":"32","author":"Antipov","year":"2016","journal-title":"Bioinformatics"},{"key":"B2","doi-asserted-by":"publisher","first-page":"192","DOI":"10.1016\/j.ijfoodmicro.2017.06.020","article-title":"Characterization of antibiotic resistant and pathogenic Escherichia coli in irrigation water and vegetables in household farms.","volume":"257","author":"Ara\u00fajo","year":"2017","journal-title":"Int. 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