{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,23]],"date-time":"2026-07-23T17:47:45Z","timestamp":1784828865342,"version":"3.55.0"},"reference-count":110,"publisher":"Frontiers Media SA","license":[{"start":{"date-parts":[[2021,5,25]],"date-time":"2021-05-25T00:00:00Z","timestamp":1621900800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["frontiersin.org"],"crossmark-restriction":true},"short-container-title":["Front. Microbiol."],"abstract":"<jats:p>\n                    <jats:italic>Borrelia burgdorferi<\/jats:italic>\n                    , the causative agent of Lyme disease, traverses through vastly distinct environments between the tick vector and the multiple phases of the mammalian infection that requires genetic adaptation for the progression of pathogenesis. Borrelial gene expression is highly responsive to changes in specific environmental signals that initiate the RpoS regulon for mammalian adaptation, but the mechanism(s) for direct detection of environmental cues has yet to be identified. Secondary messenger cyclic adenosine monophosphate (cAMP) produced by adenylate cyclase is responsive to environmental signals, such as carbon source and pH, in many bacterial pathogens to promote virulence by altering gene regulation.\n                    <jats:italic>B. burgdorferi<\/jats:italic>\n                    encodes a single non-toxin class IV adenylate cyclase (\n                    <jats:italic>bb0723<\/jats:italic>\n                    ,\n                    <jats:italic>cyaB<\/jats:italic>\n                    ). This study investigates\n                    <jats:italic>cyaB<\/jats:italic>\n                    expression along with its influence on borrelial virulence regulation and mammalian infectivity. Expression of\n                    <jats:italic>cyaB<\/jats:italic>\n                    was specifically induced with co-incubation of mammalian host cells that was not observed with cultivated tick cells suggesting that\n                    <jats:italic>cyaB<\/jats:italic>\n                    expression is influenced by cellular factor(s) unique to mammalian cell lines. The 3\u2032 end of\n                    <jats:italic>cyaB<\/jats:italic>\n                    also encodes a small RNA, SR0623, in the same orientation that overlaps with\n                    <jats:italic>bb0722<\/jats:italic>\n                    . The differential processing of\n                    <jats:italic>cyaB<\/jats:italic>\n                    and SR0623 transcripts may alter the ability to influence function in the form of virulence determinant regulation and infectivity. Two independent\n                    <jats:italic>cyaB<\/jats:italic>\n                    deletion B31 strains were generated in 5A4-NP1 and ML23 backgrounds and complemented with the\n                    <jats:italic>cyaB<\/jats:italic>\n                    ORF alone that truncates SR0623,\n                    <jats:italic>cyaB<\/jats:italic>\n                    with intact SR0623, or\n                    <jats:italic>cyaB<\/jats:italic>\n                    with a mutagenized full-length SR0623 to evaluate the influence on transcriptional and posttranscriptional regulation of borrelial virulence factors and infectivity. In the absence of\n                    <jats:italic>cyaB<\/jats:italic>\n                    , the expression and production of\n                    <jats:italic>ospC<\/jats:italic>\n                    was significantly reduced, while the protein levels for BosR and DbpA were substantially lower than parental strains. Infectivity studies with both independent\n                    <jats:italic>cyaB<\/jats:italic>\n                    mutants demonstrated an attenuated phenotype with reduced colonization of tissues during early disseminated infection. This work suggests that\n                    <jats:italic>B. burgdorferi<\/jats:italic>\n                    utilizes\n                    <jats:italic>cyaB<\/jats:italic>\n                    and potentially cAMP as a regulatory pathway to modulate borrelial gene expression and protein production to promote borrelial virulence and dissemination in the mammalian host.\n                  <\/jats:p>","DOI":"10.3389\/fmicb.2021.676192","type":"journal-article","created":{"date-parts":[[2021,5,25]],"date-time":"2021-05-25T09:17:44Z","timestamp":1621934264000},"update-policy":"https:\/\/doi.org\/10.3389\/crossmark-policy","source":"Crossref","is-referenced-by-count":6,"title":["The Borrelia burgdorferi Adenylate Cyclase, CyaB, Is Important for Virulence Factor Production and Mammalian Infection"],"prefix":"10.3389","volume":"12","author":[{"given":"Vanessa M.","family":"Ante","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lauren C.","family":"Farris","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Elizabeth P.","family":"Saputra","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Allie J.","family":"Hall","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nathaniel S.","family":"O\u2019Bier","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Adela S.","family":"Oliva Ch\u00e1vez","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Richard T.","family":"Marconi","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Meghan C.","family":"Lybecker","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jenny A.","family":"Hyde","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1965","published-online":{"date-parts":[[2021,5,25]]},"reference":[{"key":"B1","doi-asserted-by":"publisher","first-page":"775","DOI":"10.1093\/nar\/gkw1180","article-title":"In vivo expression technology and 5\u2032 end mapping of the borrelia burgdorferi transcriptome identify novel RNAs expressed during mammalian infection.","volume":"45","author":"Adams","year":"2017","journal-title":"Nucleic Acids Res."},{"key":"B2","doi-asserted-by":"publisher","DOI":"10.3390\/molecules25102462","article-title":"The world of cyclic dinucleotides in bacterial behavior.","volume":"25","author":"Aline Dias da","year":"2020","journal-title":"Molecules"},{"key":"B3","doi-asserted-by":"publisher","first-page":"43","DOI":"10.1146\/annurev-micro-020518-115907","article-title":"Posttranscription initiation control of gene expression mediated by bacterial RNA-binding proteins.","volume":"73","author":"Babitzke","year":"2019","journal-title":"Ann. 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