{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,9,27]],"date-time":"2025-09-27T13:56:59Z","timestamp":1758981419049,"version":"3.41.2"},"reference-count":30,"publisher":"Frontiers Media SA","license":[{"start":{"date-parts":[[2021,7,21]],"date-time":"2021-07-21T00:00:00Z","timestamp":1626825600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["frontiersin.org"],"crossmark-restriction":true},"short-container-title":["Front. Microbiol."],"abstract":"<jats:sec><jats:title>Background<\/jats:title><jats:p><jats:italic>Stephanoascus ciferrii<\/jats:italic> is a heterothallic ascomycetous yeast-like fungus. Recently, the concept of <jats:italic>S. ciferrii<\/jats:italic> complex has been proposed and it consists of <jats:italic>S. ciferrii<\/jats:italic>, <jats:italic>Candida allociferrii<\/jats:italic>, and <jats:italic>Candida mucifera<\/jats:italic>. We aimed to identify 32 strains of <jats:italic>S. ciferrii<\/jats:italic> complex isolated from patients with chronic suppurative otitis media (CSOM) at the species level and analyze the morphology and antifungal susceptibility profiles of the three species.<\/jats:p><\/jats:sec><jats:sec><jats:title>Method<\/jats:title><jats:p>The sequencing of the internal transcribed spacer (ITS) region and matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF MS) were used to identify <jats:italic>S. ciferrii<\/jats:italic> complex species. The SARAMIS software was used for cluster analysis of the mass spectra. All the strains were cultured on Sabouraud dextrose agar (SDA) and CHROM plates for 7 days. In the meantime, colonies of the 32 strains went through Gram staining. The Sensititre YeastOne YO10 colorimetric panel was used for the antifungal susceptibility analysis.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>There were 10 strains of <jats:italic>C. allociferrii<\/jats:italic> (31.25%), six strains of <jats:italic>C. mucifera<\/jats:italic> (18.75%), and 16 strains of <jats:italic>S. ciferrii<\/jats:italic> (50%) in the 32 strains of <jats:italic>S. ciferrii<\/jats:italic> complex according to the sequencing of the ITS region. MALDI-TOF MS could identify <jats:italic>S. ciferrii<\/jats:italic> but showed no results for <jats:italic>C. allociferrii<\/jats:italic> and <jats:italic>C. mucifera<\/jats:italic>. The cluster analysis of the mass spectra by SARAMIS indicated that the MALDI-TOF MS could distinguish the three species. The morphology characteristics of the three species were similar. As for antifungal susceptibility, <jats:italic>S. ciferrii<\/jats:italic> and <jats:italic>C. mucifera<\/jats:italic> tended to have high fluconazole MICs compared with <jats:italic>C. allociferrii<\/jats:italic>. <jats:italic>C. mucifera<\/jats:italic> and <jats:italic>C. allociferrii<\/jats:italic> had relatively low flucytosine MICs while <jats:italic>S. ciferrii<\/jats:italic> owned high flucytosine MICs. Besides, <jats:italic>C. mucifera<\/jats:italic> tended to have a higher MIC value than <jats:italic>S. ciferrii<\/jats:italic> for amphotericin B and <jats:italic>C. allociferrii<\/jats:italic> for anidulafungin, micafungin, and caspofungin.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>The antifungal susceptibility profiles of the three species of <jats:italic>S. ciferrii<\/jats:italic> complex had their own characteristics. Besides, more mass spectra of <jats:italic>C. allociferrii<\/jats:italic> and <jats:italic>C. mucifera<\/jats:italic> are needed to construct the reference database for <jats:italic>S. ciferrii<\/jats:italic> complex species, enabling MALDI-TOF MS to identify <jats:italic>S. ciferrii<\/jats:italic> complex at species level.<\/jats:p><\/jats:sec>","DOI":"10.3389\/fmicb.2021.680060","type":"journal-article","created":{"date-parts":[[2021,7,21]],"date-time":"2021-07-21T10:30:42Z","timestamp":1626863442000},"update-policy":"https:\/\/doi.org\/10.3389\/crossmark-policy","source":"Crossref","is-referenced-by-count":6,"title":["Identification and Antifungal Susceptibility Analysis of Stephanoascus ciferrii Complex Species Isolated From Patients With Chronic Suppurative Otitis Media"],"prefix":"10.3389","volume":"12","author":[{"given":"Penghao","family":"Guo","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Zhongwen","family":"Wu","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Pingjuan","family":"Liu","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yili","family":"Chen","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kang","family":"Liao","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yaqin","family":"Peng","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yuting","family":"He","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1965","published-online":{"date-parts":[[2021,7,21]]},"reference":[{"key":"B1","doi-asserted-by":"crossref","first-page":"1236","DOI":"10.1099\/jmm.0.001236","article-title":"Implication of efflux pumps and ERG11 genes in resistance of clinical Trichosporon asahii isolates to fluconazole.","volume":"70","author":"Abbes","year":"2021","journal-title":"J. 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