{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,13]],"date-time":"2026-10-13T22:14:33Z","timestamp":1791929673718,"version":"4.3.4"},"reference-count":47,"publisher":"Frontiers Media SA","license":[{"start":{"date-parts":[[2021,6,16]],"date-time":"2021-06-16T00:00:00Z","timestamp":1623801600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["frontiersin.org"],"crossmark-restriction":true},"short-container-title":["Front. Microbiol."],"abstract":"The purpose of this study was to investigate the prevalence, antimicrobial resistance, virulence genes, and genetic diversity of Campylobacter<\/jats:italic> spp. along the yellow-feathered broiler slaughtering line in Southern China from December 2018 to June 2019. A total of 157 Campylobacter<\/jats:italic> spp. isolates were identified from 1,102 samples (including 53.6% (75\/140) of live chicken anal swab samples, 27.5% (44\/160) of defeathering samples, 18.1% (29\/160) of evisceration samples, 2.1% (3\/140) of washing samples, 1.4% (2\/140) of chilling samples, and 1.1% (4\/362) of environmental samples). The prevalence of Campylobacter<\/jats:italic> spp. was 14.2%, including 43.9% Campylobacter jejuni<\/jats:italic>, 53.5% Campylobacter coli<\/jats:italic>, and 2.5% other Campylobacter<\/jats:italic> species. The highest antimicrobial resistance rate was found to be against sulfamethoxazole (138\/157, 87.9%), and 90.4% (142\/157) of the isolates were multidrug resistant (MDR). Examination of resistance-related genes revealed the double base mutated Thr-86-Ile, which informed ACA-TTA, with an Arg-79-Lys substitution in gyrA<\/jats:italic>. Eleven virulence-associated genes (cadF<\/jats:italic>, cdtA<\/jats:italic>, cdtB<\/jats:italic>, ciaB<\/jats:italic>, flaA<\/jats:italic>, imaA<\/jats:italic>, dnaJ<\/jats:italic>, plaA<\/jats:italic>, virB11<\/jats:italic>, racR<\/jats:italic>, and cdtC<\/jats:italic>) were also detected by a polymerase chain reaction (PCR) analysis, and cadF<\/jats:italic> (81.5%) was the most prevalent. Based on an analysis of pulsed-field gel electrophoresis (PFGE) results, we found that Campylobacter<\/jats:italic> spp. could be cross-contaminated throughout the entire slaughtering line. These results show that it is imperative to study the Campylobacter<\/jats:italic> spp. from the yellow-feathered broiler along the slaughtering line in China to develop preventative and treatment measures for the poultry industry, as well as food safety and public health.<\/jats:p>","DOI":"10.3389\/fmicb.2021.682741","type":"journal-article","created":{"date-parts":[[2021,6,16]],"date-time":"2021-06-16T09:40:40Z","timestamp":1623836440000},"update-policy":"https:\/\/doi.org\/10.3389\/crossmark-policy","source":"Crossref","is-referenced-by-count":18,"title":["Highly Prevalent Multidrug-Resistant Campylobacter spp. Isolated From a Yellow-Feathered Broiler Slaughterhouse in South China"],"prefix":"10.3389","volume":"12","author":[{"given":"Jie","family":"Bai","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhengquan","family":"Chen","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kaijian","family":"Luo","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fanliang","family":"Zeng","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiaoyun","family":"Qu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hongxia","family":"Zhang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kaifeng","family":"Chen","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Qijie","family":"Lin","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Haishan","family":"He","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ming","family":"Liao","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jianmin","family":"Zhang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1965","published-online":{"date-parts":[[2021,6,16]]},"reference":[{"key":"B1","doi-asserted-by":"publisher","first-page":"22","DOI":"10.1016\/j.jgar.2018.02.019","article-title":"Fluoroquinolone resistance and gyrA mutations in Campylobacter jejuni and Campylobacter coli isolated from chicken in Egypt.","volume":"13","author":"Abd El-Tawab","year":"2018","journal-title":"J. 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