{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,17]],"date-time":"2026-10-17T00:35:21Z","timestamp":1792197321671,"version":"4.4.0"},"reference-count":34,"publisher":"Frontiers Media SA","license":[{"start":{"date-parts":[[2021,6,21]],"date-time":"2021-06-21T00:00:00Z","timestamp":1624233600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["frontiersin.org"],"crossmark-restriction":true},"short-container-title":["Front. Microbiol."],"abstract":"<jats:p><jats:italic>Lactiplantibacillus plantarum<\/jats:italic> are probiotic bacteria, maintaining the integrity of the gastrointestinal epithelial barrier, and preventing the infection of pathogenic bacteria. Exopolysaccharides (EPSs) are often involved in the probiotic property of <jats:italic>L. plantarum<\/jats:italic>. Here, we identified a new EPS-synthetic gene cluster, <jats:italic>cpsWc<\/jats:italic>, carrying 13 genes, laid on a large plasmid in a well-characterized probiotic <jats:italic>L. plantarum<\/jats:italic> strain LTC-113. The <jats:italic>cpsWc<\/jats:italic> gene cluster was horizontally acquired from <jats:italic>Weissella cibaria<\/jats:italic>, enhancing the biofilm formation ability of the host strain and its tolerance to harsh environmental stresses, including heat, acid, and bile. Transfer of <jats:italic>cpsWc<\/jats:italic> also conferred the probiotic properties to other <jats:italic>L. plantarum<\/jats:italic> strains. Moreover, <jats:italic>cpsWc<\/jats:italic> strengthened the adhesion of LTC-113 to intestinal epithelial cells. Both the <jats:italic>cpsWc<\/jats:italic>-carrying LTC-113 and its EPSs <jats:italic>per se<\/jats:italic> effectively attenuated the LPS-induced pro-inflammatory effect of intestinal epithelial cells, and inhibited the adhesion of pathogenic bacteria, such as <jats:italic>S. typhimurium<\/jats:italic> and <jats:italic>E. coli<\/jats:italic> by exclusion and competition. The newly identified <jats:italic>cpsWc<\/jats:italic> gene cluster emphasized the contribution of mobile EPS-synthetic element on the probiotic activity of <jats:italic>L. plantarum<\/jats:italic>, and shed a light on the engineering of probiotic bacteria.<\/jats:p>","DOI":"10.3389\/fmicb.2021.692957","type":"journal-article","created":{"date-parts":[[2021,6,21]],"date-time":"2021-06-21T15:53:38Z","timestamp":1624290818000},"update-policy":"https:\/\/doi.org\/10.3389\/crossmark-policy","source":"Crossref","is-referenced-by-count":22,"title":["Horizontally Acquired Polysaccharide-Synthetic Gene Cluster From Weissella cibaria Boosts the Probiotic Property of Lactiplantibacillus plantarum"],"prefix":"10.3389","volume":"12","author":[{"given":"Yuqi","family":"Gao","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mingze","family":"Niu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiaohui","family":"Yu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tingting","family":"Bao","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhaowei","family":"Wu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xin","family":"Zhao","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1965","published-online":{"date-parts":[[2021,6,21]]},"reference":[{"key":"B1","doi-asserted-by":"publisher","DOI":"10.1016\/j.micpath.2019.103677","article-title":"Inhibition of Escherichia coli adhesion to human intestinal Caco-2 cells by probiotic candidate Lactobacillus plantarum strain L15.","volume":"136","author":"Alizadeh Behbahani","year":"2019","journal-title":"Microb. 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