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This use of wastewater-contaminated streams allows antibiotic-resistant bacteria to potentially cycle back to humans through agricultural products. In this study, we investigated the prevalence of extended-spectrum \u03b2-lactamase (ESBL)-producing <jats:italic>Escherichia coli<\/jats:italic> isolated from produce and irrigation water across 17 provinces of Ecuador. A total of 117 vegetable samples, 119 fruit samples, and 38 irrigation water samples were analyzed. Results showed that 11% of the samples were positive for <jats:italic>E. coli<\/jats:italic> including 11 irrigation water samples (29%), and samples of 13 vegetables (11%), and 11 fruits (9%). Among the 165 <jats:italic>E. coli<\/jats:italic> isolates cultured, 96 (58%) had the ESBL phenotype, and 58% of ESBL producing <jats:italic>E. coli<\/jats:italic> came from irrigation water samples, 11% from vegetables, and 30% from fruits. The <jats:italic>bla<\/jats:italic><jats:sub>CTX\u2013M<\/jats:sub><jats:sub>\u2013<\/jats:sub><jats:sub>55<\/jats:sub>, <jats:italic>bla<\/jats:italic><jats:sub>CTX\u2013M 65<\/jats:sub>, and <jats:italic>bla<\/jats:italic><jats:sub>CTX\u2013M 15<\/jats:sub> genes were the most frequently found gene associated with the ESBL phenotype and coincided with the <jats:italic>bla<\/jats:italic><jats:sub>CTX\u2013M<\/jats:sub> alleles associated with human infections in Ecuador. Three isolates had the mcr-1 gene which is responsible for colistin resistance. This report provides evidence of the potential role of irrigation water in the growing antimicrobial resistance crisis in Ecuador.<\/jats:p>","DOI":"10.3389\/fmicb.2021.709418","type":"journal-article","created":{"date-parts":[[2021,10,4]],"date-time":"2021-10-04T07:27:23Z","timestamp":1633332443000},"update-policy":"https:\/\/doi.org\/10.3389\/crossmark-policy","source":"Crossref","is-referenced-by-count":37,"title":["Extended-Spectrum Beta-Lactamase Producing-Escherichia coli Isolated From Irrigation Waters and Produce in Ecuador"],"prefix":"10.3389","volume":"12","author":[{"given":"Lorena","family":"Montero","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jorge","family":"Irazabal","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Paul","family":"Cardenas","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jay P.","family":"Graham","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gabriel","family":"Trueba","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1965","published-online":{"date-parts":[[2021,10,4]]},"reference":[{"key":"B1","doi-asserted-by":"publisher","first-page":"1857","DOI":"10.4315\/0362-028X.JFP-19-089","article-title":"Antibiotic resistance of Enterobacteriaceae isolated from fresh fruits and vegetables and characterization of their AmpC b-lactamases.","volume":"82","author":"Al-Kharousi","year":"2019","journal-title":"J. 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