{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,31]],"date-time":"2026-10-31T01:03:10Z","timestamp":1793408590134,"version":"4.5.0"},"reference-count":44,"publisher":"Frontiers Media SA","license":[{"start":{"date-parts":[[2021,9,1]],"date-time":"2021-09-01T00:00:00Z","timestamp":1630454400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["frontiersin.org"],"crossmark-restriction":true},"short-container-title":["Front. Microbiol."],"abstract":"<jats:p>The study investigated the impact of fermented cottonseed meal (FCSM) on growth performance, immunity and antioxidant properties, nutrient digestibility, and gut microbiota of weaned piglets by replacing soybean meal with FCSM in the diet. The experimental piglets were fed with either the soybean meal diet (SBM group) or fermented cottonseed meal diet (FCSM group) for 14days after weaning. The digestibility of dry matter (DM), organic matter (OM), crude protein (CP), gross energy (GE), amino acids and nitrogen was higher in the FCSM diet than those in the SBM diet (<jats:italic>p<\/jats:italic>&amp;lt;0.05). The piglets in the FCSM group showed greater growth performance and lower diarrhea rate than those in the SBM group (<jats:italic>p<\/jats:italic>&amp;lt;0.05). The concentration of serum immunoglobulin G (IgG) and antioxidase, intestinal and hepatic antioxidase were increased and the concentration of malondialdehyde (MDA) in the serum was decreased in those piglets in the FCSM group compared to those piglets in the SBM group (<jats:italic>p<\/jats:italic>&amp;lt;0.05). The piglets in the FCSM group had a higher concentration of volatile fatty acids (VFAs) in their ileum and cecum and a higher Simpson index of ileum than piglets in the SBM group (<jats:italic>p<\/jats:italic>&amp;lt;0.05). The relative abundance of <jats:italic>Lactobacillus<\/jats:italic> and <jats:italic>[Ruminococcus]_torques_group<\/jats:italic> in ileum and <jats:italic>Intestinibacter<\/jats:italic>, <jats:italic>norank_f_Muribaculaceae<\/jats:italic>, <jats:italic>unclassified_o_Lactobacillales<\/jats:italic> and <jats:italic>[Eubacterium]_coprostanoligenes_group<\/jats:italic> in cecum were enhanced in piglets fed with the FCSM diet, whereas the relative abundance of <jats:italic>Sarcina<\/jats:italic> and <jats:italic>Terrisporobacter<\/jats:italic> were increased in piglets fed with the SBM diet. Overall, FCSM replacing SBM improved the growth performance, immunity and antioxidant properties, and nutrient digestibility; possibly <jats:italic>via<\/jats:italic> the alterant gut microbiota and its metabolism of weaned piglets.<\/jats:p><jats:p><jats:fig><jats:label>Graphical Abstract<\/jats:label><jats:caption><jats:p>Fermented cottonseed meal as a partial replacement for soybean meal could improve the growth performance, immunity and antioxidant properties, and nutrient digestibility by altering the gut microbiota profile of weaned piglets. SBM, soybean meal; FCSM, fermented cottonseed meal.<\/jats:p><\/jats:caption><\/jats:fig><\/jats:p>","DOI":"10.3389\/fmicb.2021.734389","type":"journal-article","created":{"date-parts":[[2021,11,4]],"date-time":"2021-11-04T14:50:57Z","timestamp":1636037457000},"update-policy":"https:\/\/doi.org\/10.3389\/crossmark-policy","source":"Crossref","is-referenced-by-count":40,"title":["Fermented Cottonseed Meal as a Partial Replacement for Soybean Meal Could Improve the Growth Performance, Immunity and Antioxidant Properties, and Nutrient Digestibility by Altering the Gut Microbiota Profile of Weaned Piglets"],"prefix":"10.3389","volume":"12","author":[{"given":"Xueling","family":"Gu","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhiqing","family":"Li","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jing","family":"Wang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jiashun","family":"Chen","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Qian","family":"Jiang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nian","family":"Liu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiang","family":"Liu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fan","family":"Zhang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bie","family":"Tan","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hao","family":"Li","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiaokang","family":"Ma","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1965","published-online":{"date-parts":[[2021,9,1]]},"reference":[{"key":"ref1","doi-asserted-by":"publisher","first-page":"379","DOI":"10.1016\/j.aninu.2020.08.001","article-title":"Opportunities of prebiotics for the intestinal health of monogastric animals","volume":"6","author":"Azad","year":"2020","journal-title":"Anim. Nutr."},{"key":"ref2","doi-asserted-by":"publisher","first-page":"994","DOI":"10.1111\/are.12040","article-title":"Effects of partial substitution of dietary fish meal by fermented soybean meal on growth performance, amino acid and biochemical parameters of juvenile black sea bream acanthopagrus schlegeli","volume":"45","author":"Azarm","year":"2014","journal-title":"Aquac. Res."},{"key":"ref3","doi-asserted-by":"publisher","first-page":"1688","DOI":"10.3389\/fmicb.2017.01688","article-title":"The maturing development of gut microbiota in commercial piglets during the weaning transition","volume":"8","author":"Chen","year":"2017","journal-title":"Front. Microbiol."},{"key":"ref4","doi-asserted-by":"publisher","first-page":"680","DOI":"10.1111\/jpn.12122","article-title":"Effects of sodium butyrate on growth performance, haematological and immunological characteristics of weanling piglets","volume":"98","author":"Fang","year":"2014","journal-title":"J. Anim. Physiol. An. N."},{"key":"ref5","doi-asserted-by":"publisher","first-page":"72","DOI":"10.1016\/j.aninu.2020.06.010","article-title":"Isomaltooligosaccharide and bacillus regulate the duration of farrowing and weaning-estrous interval in sows during the perinatal period by changing the gut microbiota of sows","volume":"7","author":"Gu","year":"2021","journal-title":"Anim. Nutr."},{"key":"ref6","doi-asserted-by":"publisher","first-page":"9","DOI":"10.13257\/j.cnki.21-1104\/s.2017.04.003","article-title":"Effects of fermented cottonseed meal on production performance and health status of nursery pig, growing pig and finishing pig","volume":"4","author":"Guan","year":"2017","journal-title":"Swine Production"},{"key":"ref7","doi-asserted-by":"publisher","first-page":"187","DOI":"10.1016\/j.vetmic.2019.02.003","article-title":"Lactobacillus johnsonii L531 reduces pathogen load and helps maintain short-chain fatty acid levels in the intestines of pigs challenged with salmonella enterica Infantis","volume":"230","author":"He","year":"2019","journal-title":"Vet. Microbiol."},{"key":"ref8","doi-asserted-by":"publisher","first-page":"174","DOI":"10.1038\/nature09944","article-title":"Enterotypes of the human gut microbiome","volume":"473","author":"Jamet","year":"2011","journal-title":"Nature"},{"key":"ref9","doi-asserted-by":"publisher","first-page":"18","DOI":"10.1016\/j.anifeedsci.2015.12.002","article-title":"Dietary fiber and protein fermentation in the intestine of swine and their interactive effects on gut health and on the environment: a review","volume":"212","author":"Jha","year":"2016","journal-title":"Anim. Feed Sci. Technol."},{"key":"ref10","doi-asserted-by":"publisher","first-page":"503","DOI":"10.1007\/s00253-012-4497-y","article-title":"Biotechnology of non-saccharomyces yeasts-the basidiomycetes","volume":"97","author":"Johnson","year":"2013","journal-title":"Appl. Microbiol. Biotechnol."},{"key":"ref11","doi-asserted-by":"publisher","first-page":"282","DOI":"10.2527\/jas1977.442282x","article-title":"Evaluation of acid-insoluble ash as a natural marker in ruminant digestibility studies","volume":"44","author":"Keulen","year":"1977","journal-title":"J. Anim. Sci."},{"key":"ref12","doi-asserted-by":"publisher","first-page":"311","DOI":"10.1080\/09540105.2013.791969","article-title":"Identification of a second major antigenic epitope in the \u03b1-subunit of soy \u03b2-conglycinin","volume":"25","author":"Kim","year":"2014","journal-title":"Food Agric. Immunol."},{"key":"ref13","doi-asserted-by":"publisher","first-page":"15","DOI":"10.1080\/09064702.2013.877520","article-title":"Effect of dietary supplementation of combination of probiotics on the growth performance and immune response of broiler chickens","volume":"13","author":"Latesh","year":"2013","journal-title":"Anim. Nutr. Feed. Technol."},{"key":"ref14","doi-asserted-by":"publisher","first-page":"639039","DOI":"10.3389\/fvets.2021.639039","article-title":"Enzyme-treated soybean meal replacing extruded full-fat soybean affects nitrogen digestibility, cecal fermentation characteristics and bacterial community of newly weaned piglets","volume":"8","author":"Li","year":"2021","journal-title":"Front. Vet. Sci."},{"key":"ref15","doi-asserted-by":"publisher","first-page":"161","DOI":"10.1186\/s40168-020-00917-7","article-title":"Spatial heterogeneity of bacterial colonization across different gut segments following inter-species microbiota transplantation","volume":"8","author":"Li","year":"2020","journal-title":"Microbiome"},{"key":"ref16","doi-asserted-by":"publisher","first-page":"494","DOI":"10.1111\/j.1749-7345.2011.00491.x","article-title":"A microbial fermentation of soybean and cottonseed meal increases antioxidant activity and gossypol detoxification in diets for Nile tilapia, Oreochromis niloticus","volume":"42","author":"Lim","year":"2011","journal-title":"J. World Aquacult. Soc."},{"key":"ref17","doi-asserted-by":"publisher","first-page":"5533210","DOI":"10.1155\/2021\/5533210","article-title":"Influences of selenium-enriched yeast on growth performance, immune function, and antioxidant capacity in weaned pigs exposure to oxidative stress","volume":"2021","author":"Liu","year":"2021","journal-title":"Biomed. Res. Int."},{"key":"ref18","doi-asserted-by":"publisher","first-page":"582","DOI":"10.1093\/ps\/86.3.582","article-title":"Relative toxicity of gossypol enantiomers in laying and broiler breeder hens","volume":"86","author":"Lordelo","year":"2007","journal-title":"Poult. Sci."},{"key":"ref19","doi-asserted-by":"publisher","first-page":"2620","DOI":"10.3389\/fmicb.2019.02620","article-title":"Effect of fermented corn-soybean meal on serum immunity, the expression of genes related to gut immunity, gut microbiota, and bacterial metabolites in grower-finisher pigs","volume":"10","author":"Lu","year":"2019","journal-title":"Front. Microbiol."},{"key":"ref20","doi-asserted-by":"publisher","first-page":"39","DOI":"10.1016\/j.livsci.2019.04.016","article-title":"Effects of replacing soybean meal, soy protein concentrate, fermented soybean meal or fish meal with enzyme-treated soybean meal on growth performance, nutrient digestibility, antioxidant capacity, immunity and intestinal morphology in weaned pigs","volume":"225","author":"Ma","year":"2019","journal-title":"Livest. Sci."},{"key":"ref21","doi-asserted-by":"publisher","first-page":"47","DOI":"10.1016\/j.anifeedsci.2018.12.003","article-title":"Comparative effects of enzymolytic soybean meal and antibiotics in diets on growth performance, antioxidant capacity, immunity, and intestinal barrier function in weaned pigs","volume":"248","author":"Ma","year":"2019","journal-title":"Anim. Feed Sci. Technol."},{"key":"ref22","doi-asserted-by":"publisher","first-page":"119","DOI":"10.2141\/jpsa.44.119","article-title":"Cottonseed meal in poultry diets: a review","volume":"44","author":"Nagalakshmi","year":"2007","journal-title":"J. Poult. Sci."},{"key":"ref23","doi-asserted-by":"publisher","first-page":"255","DOI":"10.1080\/09637486.2018.1499711","article-title":"Dietary protein source and butyrylated high-amylose maize starch included in a high-protein diet determines the urinary metabolome of rats","volume":"70","author":"Najafabadi","year":"2019","journal-title":"Int. J. Food Sci. Nutr."},{"key":"ref24","doi-asserted-by":"publisher","first-page":"350","DOI":"10.3906\/vet-1410-65","article-title":"Effects of fermented cottonseed meal on the growth performance, apparent digestibility, carcass traits, and meat composition in yellow-feathered broilers","volume":"39","author":"Nie","year":"2015","journal-title":"Turk. J. V. Anim. Sci."},{"key":"ref25","doi-asserted-by":"publisher","first-page":"4345","DOI":"10.1007\/s00253-020-10538-7","article-title":"Cottonseed meal fermented by Candida tropical reduces the fat deposition in white-feather broilers through cecum bacteria-host metabolic cross-talk","volume":"104","author":"Niu","year":"2020","journal-title":"Appl. Microbiol. Biotechnol."},{"key":"ref44","year":"2012"},{"key":"ref26","doi-asserted-by":"publisher","first-page":"430","DOI":"10.1128\/IAI.02245-14","article-title":"Neutralization of mitochondrial superoxide by superoxide dismutase 2 promotes bacterial clearance and regulates phagocyte numbers in zebrafish","volume":"83","author":"Peterman","year":"2015","journal-title":"Infect. Immun."},{"key":"ref27","doi-asserted-by":"publisher","first-page":"54","DOI":"10.1016\/j.anifeedsci.2018.09.005","article-title":"The impact of reducing dietary crude protein and increasing total dietary fiber on hindgut fermentation, the methanogen community and gas emission in growing pigs","volume":"245","author":"Seradj","year":"2018","journal-title":"Anim. Feed Sci. Technol."},{"key":"ref28","doi-asserted-by":"publisher","first-page":"813","DOI":"10.1080\/09540105.2015.1036356","article-title":"Expression of cytokines in chicken peripheral blood mononuclear cells after stimulation by probiotic bacteria and campylobacter jejuni in vitro","volume":"26","author":"Sta\u0161ov\u00e1","year":"2015","journal-title":"Food Agric. Immunol."},{"key":"ref29","doi-asserted-by":"publisher","first-page":"282","DOI":"10.1016\/j.livsci.2007.01.019","article-title":"Definition of apparent, true, and standardized ileal digestibility of amino acids in pigs","volume":"109","author":"Stein","year":"2007","journal-title":"Livest. Sci."},{"key":"ref30","doi-asserted-by":"publisher","first-page":"987","DOI":"10.1007\/s11250-012-0322-y","article-title":"Effects of dietary inclusion of fermented cottonseed meal on growth, cecal microbial population, small intestinal morphology, and digestive enzyme activity of broilers","volume":"45","author":"Sun","year":"2013","journal-title":"Trop. Anim. Health Prod."},{"key":"ref31","doi-asserted-by":"publisher","first-page":"264","DOI":"10.1080\/19476337.2014.948072","article-title":"Chemical composition and in vitro antioxidant property of peptides produced from cottonseed meal by solid-state fermentation","volume":"13","author":"Sun","year":"2014","journal-title":"CYTA J. Food"},{"key":"ref32","doi-asserted-by":"publisher","first-page":"473","DOI":"10.1017\/S0043933916000258","article-title":"The use of cottonseed meal as a protein source for poultry: an updated review","volume":"72","author":"\u015awi\u0105tkiewicz","year":"2016","journal-title":"Worlds Poult. Sci. J."},{"key":"ref33","doi-asserted-by":"publisher","first-page":"393","DOI":"10.5713\/ajas.2011.11381","article-title":"Effects of replacement of soybean meal by fermented cottonseed meal on growth performance, serum biochemical parameters and immune function of yellow-feathered broilers","volume":"25","author":"Tang","year":"2012","journal-title":"Asian Aust. J. Anim. Sci."},{"key":"ref34","doi-asserted-by":"publisher","first-page":"76","DOI":"10.1093\/jas\/skz122.141","article-title":"Effect of sodium butyrate (SB) on growth performance and complete blood cell count in nursery pigs: two facility study","volume":"97","author":"Tsai","year":"2019","journal-title":"J. Anim. Sci."},{"key":"ref35","doi-asserted-by":"publisher","first-page":"310","DOI":"10.3390\/nu9030310","article-title":"Dietary protein and amino acid supplementation in inflammatory bowel disease course: what impact on the colonic mucosa","volume":"9","author":"Vidal-Lletj\u00f3s","year":"2017","journal-title":"Nutrients"},{"key":"ref36","doi-asserted-by":"publisher","first-page":"725","DOI":"10.1080\/09540105.2017.1311308","article-title":"Effects of fermented cottonseed meal on growth performance, serum biochemical parameters, immune functions, antioxidative abilities, and cecal microflora in broilers","volume":"28","author":"Wang","year":"2017","journal-title":"Food Agric. Immunol."},{"key":"ref37","doi-asserted-by":"publisher","first-page":"79","DOI":"10.1186\/s40104-019-0387-9","article-title":"Low-protein diets supplemented with casein hydrolysate favor the microbiota and enhance the mucosal humoral immunity in the colon of pigs","volume":"10","author":"Wang","year":"2019","journal-title":"J. Anim. Sci. Biotechnol."},{"key":"ref38","doi-asserted-by":"publisher","first-page":"109","DOI":"10.1186\/s40168-019-0721-7","article-title":"Longitudinal investigation of the swine gut microbiome from birth to market reveals stage and growth performance associated bacteria","volume":"7","author":"Wang","year":"2019","journal-title":"Microbiome"},{"key":"ref39","doi-asserted-by":"publisher","first-page":"26","DOI":"10.1186\/s13567-020-00754-4","article-title":"Anti-inflammatory effects of Lactobacillus johnsonii L531 in a pig model of Salmonella Infantis infection involves modulation of CCR6 + T cell responses and ER stress","volume":"51","author":"Yang","year":"2020","journal-title":"Vet. Res."},{"key":"ref40","doi-asserted-by":"publisher","first-page":"690","DOI":"10.1631\/jzus.2006.B0690","article-title":"Effect of selected fungi on the reduction of gossypol levels and nutritional value during solid substrate fermentation of cottonseed meal","volume":"7","author":"Zhang","year":"2006","journal-title":"J. Zhejiang Univ. Sci. B"},{"key":"ref41","doi-asserted-by":"publisher","first-page":"176","DOI":"10.1016\/j.anifeedsci.2006.06.003","article-title":"Development of a microbial fermentation process for detoxification of gossypol in cottonseed meal","volume":"135","author":"Zhang","year":"2007","journal-title":"Anim. Feed Sci. Technol."},{"key":"ref42","doi-asserted-by":"publisher","first-page":"7995","DOI":"10.1021\/acs.jafc.8b02545","article-title":"Dietary fiber increases the butyrate-producing bacteria and improves growth performance of weaned piglets","volume":"66","author":"Zhao","year":"2018","journal-title":"J. Agric. Food Chem."},{"key":"ref43","doi-asserted-by":"publisher","first-page":"39","DOI":"10.1016\/j.foodres.2016.08.042","article-title":"Formation of taste-active amino acids, amino acid derivatives and peptides in food fermentations-a review","volume":"89","author":"Zhao","year":"2016","journal-title":"Food Res. 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