{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,23]],"date-time":"2026-04-23T07:48:34Z","timestamp":1776930514573,"version":"3.51.2"},"reference-count":32,"publisher":"Frontiers Media SA","license":[{"start":{"date-parts":[[2021,10,14]],"date-time":"2021-10-14T00:00:00Z","timestamp":1634169600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["frontiersin.org"],"crossmark-restriction":true},"short-container-title":["Front. Microbiol."],"abstract":"<jats:p>Gonorrhea is a sexually transmitted disease caused by the host-adapted human pathogen, <jats:italic>Neisseria gonorrhoeae<\/jats:italic>. The morbidity is increasing and poses a major public health concern, especially in resource-scarce regions. Therefore, a rapid, visual, sensitive, specific, cost-saving, and simple assay for <jats:italic>N. gonorrhoeae<\/jats:italic> detection is critical for prompt treatment and the prevention of further transmission. Here, for the first time, we report a novel assay called the multiple cross displacement amplification combined with gold nanoparticle-based lateral flow biosensor (MCDA-LFB), which we constructed for the rapid and visual identification of <jats:italic>N. gonorrhoeae<\/jats:italic> in clinical samples. We successfully devised a set of MCDA primers based on the <jats:italic>N. gonorrhoeae<\/jats:italic>-specific gene, <jats:italic>orf1<\/jats:italic>. Optimal assay conditions were determined at 67\u00b0C, including genomic DNA preparation (\u223c15 min), MCDA amplification (30 min), and LFB reading (\u223c2 min), which can be completed within 50 min. The limit of detection (LoD) of the assay was 20 copies\/test (in a 25-\u03bcl reaction mixture). Assay specificity was 100%, with no cross-reactions with other pathogens. Thus, our <jats:italic>N. gonorrhoeae<\/jats:italic>-MCDA-LFB is a rapid, specific, visual, cost-saving, and easy-to-use assay for <jats:italic>N. gonorrhoeae<\/jats:italic> diagnostics, and may have great potential for point-of-care (POC) testing in clinical settings, especially in resource-limited regions.<\/jats:p>","DOI":"10.3389\/fmicb.2021.747140","type":"journal-article","created":{"date-parts":[[2021,10,14]],"date-time":"2021-10-14T08:26:12Z","timestamp":1634199972000},"update-policy":"https:\/\/doi.org\/10.3389\/crossmark-policy","source":"Crossref","is-referenced-by-count":7,"title":["A Nanoparticle-Based Biosensor Combined With Multiple Cross Displacement Amplification for the Rapid and Visual Diagnosis of Neisseria gonorrhoeae in Clinical Application"],"prefix":"10.3389","volume":"12","author":[{"given":"Xu","family":"Chen","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Liming","family":"Huang","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Qingxue","family":"Zhou","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yan","family":"Tan","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xuhong","family":"Tan","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Shilei","family":"Dong","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1965","published-online":{"date-parts":[[2021,10,14]]},"reference":[{"key":"B1","doi-asserted-by":"publisher","first-page":"400","DOI":"10.12688\/f1000research.13600.1","article-title":"Epidemiological, behavioural, and clinical factors associated with antimicrobial-resistant gonorrhoea: a review.","volume":"7","author":"Abraha","year":"2018","journal-title":"F1000Res"},{"key":"B2","doi-asserted-by":"publisher","first-page":"5758387","DOI":"10.1155\/2016\/5758387","article-title":"Extragenital infections caused by Chlamydia trachomatis and Neisseria gonorrhoeae: a review of the literature.","volume":"2016","author":"Chan","year":"2016","journal-title":"Infect. 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