{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,11]],"date-time":"2026-03-11T17:52:42Z","timestamp":1773251562950,"version":"3.50.1"},"reference-count":28,"publisher":"Frontiers Media SA","license":[{"start":{"date-parts":[[2022,1,6]],"date-time":"2022-01-06T00:00:00Z","timestamp":1641427200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["frontiersin.org"],"crossmark-restriction":true},"short-container-title":["Front. Microbiol."],"abstract":"<jats:p>A rectal swab sample was collected from a patient with Guillain\u2013Barr\u00e9 syndrome and enriched in lysogeny broth. Carbapenem-resistant bacteria were selected by China Blue agar plates containing 0.3 \u03bcg\/ml meropenem. Carbapenemase-producing <jats:italic>Klebsiella michiganensis<\/jats:italic> was identified and characterized by matrix-assisted laser desorption\/ionization time-of-flight (MALDI-TOF), immune colloidal gold technique, a conjugation experiment, PCR analysis, and antimicrobial susceptibility testing. The genome of <jats:italic>K. michiganensis<\/jats:italic> was determined by whole genome sequencing. Antimicrobial susceptibility testing showed that the <jats:italic>K. michiganensis<\/jats:italic> was resistant to imipenem, meropenem, ertapenem, cefmetazole, ceftazidime, cefotaxime, piperacillin\/tazobactam, sulbactam\/cefoperazone, ceftazidime\/avibactam, cefepime, and aztreonam while susceptible to polymyxin B, ciprofloxacin, tigecycline, and amikacin. Immune colloidal gold technique suggested that this strain co-produced three different carbapenemases [<jats:italic>Klebsiella pneumoniae<\/jats:italic> carbapenemase (KPC), New Delhi metallo-beta-lactamase (NDM), and Imipenem (IMP)]. Whole genome sequencing analysis indicated that this strain belonged to ST91, and <jats:italic>bla<\/jats:italic><jats:sub>KPC\u20132<\/jats:sub>, <jats:italic>bla<\/jats:italic><jats:sub>NDM\u20131<\/jats:sub>, and <jats:italic>bla<\/jats:italic><jats:sub>IMP\u20134<\/jats:sub> were carried on different conjugative plasmids. Besides, the co-existence and transferability of <jats:italic>bla<\/jats:italic><jats:sub>KPC\u20132<\/jats:sub>, <jats:italic>bla<\/jats:italic><jats:sub>NDM\u20131<\/jats:sub>, and <jats:italic>bla<\/jats:italic><jats:sub>IMP\u20134<\/jats:sub> in <jats:italic>K. michiganensis<\/jats:italic> facilitates the potential horizontal dissemination and nosocomial spread of resistance genes among multidrug-resistant organisms.<\/jats:p>","DOI":"10.3389\/fmicb.2021.762509","type":"journal-article","created":{"date-parts":[[2022,1,6]],"date-time":"2022-01-06T06:43:05Z","timestamp":1641451385000},"update-policy":"https:\/\/doi.org\/10.3389\/crossmark-policy","source":"Crossref","is-referenced-by-count":17,"title":["Emergence and Genomic Characterization of a KPC-2-, NDM-1-, and IMP-4-Producing Klebsiella michiganensis Isolate"],"prefix":"10.3389","volume":"12","author":[{"given":"Yanyan","family":"Zhang","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Danxia","family":"Gu","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xuemei","family":"Yang","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yuchen","family":"Wu","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Congcong","family":"Liu","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Zhangqi","family":"Shen","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Rong","family":"Zhang","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1965","published-online":{"date-parts":[[2022,1,6]]},"reference":[{"key":"B1","doi-asserted-by":"publisher","DOI":"10.1186\/1471-2164-12-402","article-title":"BLAST Ring Image Generator (BRIG): simple prokaryote genome comparisons.","volume":"12","author":"Alikhan","year":"2011","journal-title":"BMC Genomics"},{"key":"B2","doi-asserted-by":"publisher","first-page":"310","DOI":"10.1016\/j.jgar.2021.03.027","article-title":"First report of a bla(VIM-1) metallo-\u03b2-lactamase-possessing Klebsiella michiganensis.","volume":"25","author":"Campos-Madueno","year":"2021","journal-title":"J. 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