{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,5]],"date-time":"2026-10-05T20:08:21Z","timestamp":1791230901628,"version":"4.1.0"},"reference-count":93,"publisher":"Frontiers Media SA","license":[{"start":{"date-parts":[[2021,12,15]],"date-time":"2021-12-15T00:00:00Z","timestamp":1639526400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["frontiersin.org"],"crossmark-restriction":true},"short-container-title":["Front. Microbiol."],"abstract":"<jats:p>\n                    Excessive, long-term application of chemical fertilizers in sugarcane crops disrupts soil microbial flora and causes environmental pollution and yield decline. The role of endophytic bacteria in improving crop production is now well-documented. In this study, we have isolated and identified several endophytic bacterial strains from the root tissues of five sugarcane species. Among them, eleven Gram-negative isolates were selected and screened for plant growth-promoting characteristics, i.e., production of siderophores, indole-3-acetic acid (IAA), ammonia, hydrogen cyanide (HCN), and hydrolytic enzymes, phosphorus solubilization, antifungal activity against plant pathogens, nitrogen-fixation, 1-aminocyclopropane-1-carboxylic acid deaminase activity, and improving tolerance to different abiotic stresses. These isolates had\n                    <jats:italic>nifH<\/jats:italic>\n                    (11 isolates),\n                    <jats:italic>acdS<\/jats:italic>\n                    (8 isolates), and\n                    <jats:italic>HCN<\/jats:italic>\n                    (11 isolates) genes involved in N-fixation, stress tolerance, and pathogen biocontrol, respectively. Two isolates\n                    <jats:italic>Pantoea cypripedii<\/jats:italic>\n                    AF1and\n                    <jats:italic>Kosakonia arachidis<\/jats:italic>\n                    EF1 were the most potent strains and they colonized and grew in sugarcane plants. Both strains readily colonized the leading Chinese sugarcane variety GT42 and significantly increased the activity of nitrogen assimilation enzymes (glutamine synthetase, NADH glutamate dehydrogenase, and nitrate reductase), chitinase, and endo-glucanase and the content of phytohormones gibberellic acid, indole-3-acetic acid, and abscisic acid. The gene expression analysis of GT42 inoculated with isolates of\n                    <jats:italic>P. cypripedii<\/jats:italic>\n                    AF1 or\n                    <jats:italic>K. arachidis<\/jats:italic>\n                    EF1 showed increased activity of\n                    <jats:italic>nifH<\/jats:italic>\n                    and nitrogen assimilation genes. Also, the inoculated diazotrophs significantly increased plant nitrogen content, which was corroborated by the\n                    <jats:sup>15<\/jats:sup>\n                    N isotope dilution analysis. Collectively, these findings suggest that\n                    <jats:italic>P. cypripedii<\/jats:italic>\n                    and\n                    <jats:italic>K. arachidis<\/jats:italic>\n                    are beneficial endophytes that could be used as a biofertilizer to improve plant nitrogen nutrition and growth of sugarcane. To the best of our knowledge, this is the first report of sugarcane growth enhancement and nitrogen fixation by Gram-negative sugarcane root-associated endophytic bacteria\n                    <jats:italic>P. cypripedii<\/jats:italic>\n                    and\n                    <jats:italic>K. arachidis<\/jats:italic>\n                    . These strains have the potential to be utilized as sugarcane biofertilizers, thus reducing nitrogen fertilizer use and improving disease management.\n                  <\/jats:p>","DOI":"10.3389\/fmicb.2021.774707","type":"journal-article","created":{"date-parts":[[2021,12,15]],"date-time":"2021-12-15T04:31:13Z","timestamp":1639542673000},"update-policy":"https:\/\/doi.org\/10.3389\/crossmark-policy","source":"Crossref","is-referenced-by-count":57,"title":["Root-Derived Endophytic Diazotrophic Bacteria Pantoea cypripedii AF1 and Kosakonia arachidis EF1 Promote Nitrogen Assimilation and Growth in Sugarcane"],"prefix":"10.3389","volume":"12","author":[{"given":"Rajesh Kumar","family":"Singh","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Pratiksha","family":"Singh","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dao-Jun","family":"Guo","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Anjney","family":"Sharma","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dong-Ping","family":"Li","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiang","family":"Li","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Krishan K.","family":"Verma","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mukesh Kumar","family":"Malviya","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiu-Peng","family":"Song","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Prakash","family":"Lakshmanan","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Li-Tao","family":"Yang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yang-Rui","family":"Li","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1965","published-online":{"date-parts":[[2021,12,15]]},"reference":[{"key":"B1","doi-asserted-by":"publisher","first-page":"196","DOI":"10.1111\/1751-7915.12117","article-title":"Siderophores in environmental research: roles and applications.","volume":"7","author":"Ahmed","year":"2014","journal-title":"Microbial Biotechnol."},{"key":"B2","doi-asserted-by":"publisher","first-page":"645","DOI":"10.1007\/s10886-020-01209-y","article-title":"Chemical ecology of multitrophic microbial interactions: plants, insects, microbes and the metabolites that connect them.","volume":"46","author":"Ali","year":"2020","journal-title":"J. Chem. Ecol."},{"key":"B3","doi-asserted-by":"crossref","first-page":"283","DOI":"10.1080\/00380768.2002.10409202","article-title":"Estimation of the nitrogen fixation by sugarcane cultivars NiF-8 using 15N dilution and natural 15N abundance techniques.","volume":"48","author":"Asis","year":"2002","journal-title":"Soil Sci. Plant Nutr."},{"key":"B4","doi-asserted-by":"publisher","first-page":"W147","DOI":"10.1093\/nar\/gkw419","article-title":"Heatmapper: web-enabled heat mapping for all.","volume":"44","author":"Babicki","year":"2016","journal-title":"Nucleic Acids Res."},{"key":"B5","doi-asserted-by":"publisher","first-page":"189","DOI":"10.1139\/w02-007","article-title":"Response of spring rape (Brassica napus L. var. Oleifera) to inoculation with plant growth promoting rhizobacteria containing 1-aminocyclopropane-1-carboxylate deaminase depends on nutrient status of the plant.","volume":"48","author":"Belimov","year":"2002","journal-title":"Can. J. Microbiol."},{"key":"B6","doi-asserted-by":"publisher","first-page":"487","DOI":"10.1111\/1751-7915.12159","article-title":"Vegetable microbiomes: is there a connection among opportunistic infections, human health and our \u201cgut feeling\u201d?","volume":"7","author":"Berg","year":"2014","journal-title":"Microb. Biotechnol."},{"key":"B7","doi-asserted-by":"publisher","DOI":"10.1186\/1475-2859-13-66","article-title":"Biofertilizers function as key player in sustainable agriculture by improving soil fertility, plant tolerance and crop productivity.","volume":"13","author":"Bhardwaj","year":"2014","journal-title":"Microbial Cell Factories"},{"key":"B8","doi-asserted-by":"publisher","first-page":"1383","DOI":"10.1007\/s00425-018-2964-0","article-title":"Plant growth-promoting bacteria Kosakonia radicincitans mediate anti-herbivore defense in Arabidopsis thaliana.","volume":"248","author":"Brock","year":"2018","journal-title":"Planta"},{"key":"B9","doi-asserted-by":"publisher","DOI":"10.1007\/s13205-018-1488-4","article-title":"Characterization of mineral phosphate solubilizing and plant growth promoting bacteria from termite soil of arid region.","volume":"8","author":"Chakdar","year":"2018","journal-title":"3 Biotech"},{"key":"B10","article-title":"Pantoea alhagi, a novel endophytic bacterium with ability to improve growth and drought tolerance in wheat.","volume":"7","author":"Chen","year":"2017","journal-title":"Sci. Rep."},{"key":"B11","doi-asserted-by":"publisher","DOI":"10.3389\/fmicb.2019.02171","article-title":"Identification and characterization of the phosphate-solubilizing bacterium Pantoea sp. S32 in reclamation Soil in Shanxi, China.","volume":"10","author":"Chen","year":"2019","journal-title":"Front. Microbiol."},{"key":"B12","doi-asserted-by":"publisher","first-page":"425","DOI":"10.1007\/s12223-017-0513-z","article-title":"Rhizobia: a potential biocontrol agent for soilborne fungal pathogens.","volume":"62","author":"Das","year":"2017","journal-title":"Folia Microbiol."},{"key":"B13","doi-asserted-by":"publisher","first-page":"671","DOI":"10.1007\/978-94-017-2416-6_55","article-title":"Endophytic diazotrophs in sugar cane, cereals and tuber plants","author":"Dobereiner","year":"1993","journal-title":"New Horizons in Nitrogen Fixation"},{"key":"B14","doi-asserted-by":"publisher","first-page":"7843","DOI":"10.1093\/nar\/17.19.7843","article-title":"Isolation and direct complete nucleotide determination of entire genes. characterization of a gene coding for 16S ribosomal RNA.","volume":"17","author":"Edwards","year":"1989","journal-title":"Nucleic Acids Res."},{"key":"B15","year":"","journal-title":"FAO Agricultural Outlook. Agriculture Statistics."},{"key":"B16","year":"","journal-title":"World Fertilizer Trends and Outlook to 2018."},{"key":"B17","doi-asserted-by":"publisher","first-page":"783","DOI":"10.1111\/j.1558-5646.1985.tb00420.x","article-title":"Confidence limits on phylogenies, an approach using the bootstrap.","volume":"39","author":"Felsenstein","year":"1985","journal-title":"Evolution"},{"key":"B18","doi-asserted-by":"publisher","DOI":"10.1101\/cshperspect.a015669","article-title":"Opportunistic infections\u2013coming to the limits of immune suppression?","volume":"3","author":"Fishman","year":"2013","journal-title":"Cold Spring Harb. Perspect. Med."},{"key":"B19","doi-asserted-by":"crossref","first-page":"375","DOI":"10.1016\/S0021-9258(18)84756-1","article-title":"A colorimetric determination of phosphorus.","volume":"66","author":"Fiske","year":"1925","journal-title":"J. Biol. Chem."},{"key":"B20","doi-asserted-by":"crossref","first-page":"278","DOI":"10.1016\/j.oneear.2019.10.022","article-title":"Agriculture 5.0: reconciling production with planetary health.","volume":"1","author":"Fraser","year":"2019","journal-title":"One Earth"},{"key":"B21","doi-asserted-by":"publisher","first-page":"934","DOI":"10.1093\/pcp\/pct046","article-title":"Cytosolic glutamine synthetase1;2 is responsible for the primary assimilation of ammonium in rice roots.","volume":"54","author":"Funayama","year":"2013","journal-title":"Plant Cell Physiol."},{"key":"B22","doi-asserted-by":"publisher","first-page":"30","DOI":"10.1016\/j.micres.2013.09.009","article-title":"Bacteria with ACC deaminase can promote plant growth and help to feed the world.","volume":"169","author":"Glick","year":"2014","journal-title":"Microbiol. Res."},{"key":"B23","doi-asserted-by":"crossref","first-page":"227","DOI":"10.1080\/07352680701572966","article-title":"Promotion of plant growth by bacterial ACC deaminase.","volume":"26","author":"Glick","year":"2007","journal-title":"Crit. Rev. Plant Sci."},{"key":"B24","doi-asserted-by":"crossref","first-page":"192","DOI":"10.1104\/pp.26.1.192","article-title":"Colorimetric estimation of indole acetic acid.","volume":"26","author":"Gordon","year":"1951","journal-title":"Plant Physiol."},{"key":"B25","doi-asserted-by":"publisher","first-page":"66","DOI":"10.1016\/j.micres.2013.07.004","article-title":"Screening of PGPR from saline desert of Kutch: growth promotion in Arachis hypogea by Bacillus licheniformis A2.","volume":"169","author":"Goswami","year":"2014","journal-title":"Microbiol. Res."},{"key":"B26","doi-asserted-by":"crossref","first-page":"165","DOI":"10.1016\/S1360-1385(03)00051-7","article-title":"Dissecting Arabidopsis lateral root development.","volume":"8","author":"Graham","year":"2003","journal-title":"Trends Plant Sci."},{"key":"B27","doi-asserted-by":"publisher","DOI":"10.3389\/fmicb.2020.580081","article-title":"Complete genome sequence of Enterobacter roggenkampii ED5, a nitrogen fixing plant growth promoting endophytic bacterium with biocontrol and stress tolerance properties, isolated from sugarcane root.","volume":"11","author":"Guo","year":"2020","journal-title":"Front. Microbiol."},{"key":"B28","doi-asserted-by":"publisher","first-page":"1185","DOI":"10.1104\/pp.43.8.1185","article-title":"The acetylene ethylene assay for N fixation, laboratory and field evaluation.","volume":"43","author":"Hardy","year":"1968","journal-title":"Plant Physiol."},{"key":"B29","first-page":"1825","article-title":"Metabolism of 1-aminocyclopropane-1- carboxylic acid.","volume":"42","author":"Honma","year":"1978","journal-title":"Agric. Biol. Chem."},{"key":"B30","first-page":"4321","article-title":"A simple double-layered chrome azurol S agar (SDCASA) plate assay to optimize the production of siderophores by a potential biocontrol agent Bacillus.","volume":"5","author":"Hu","year":"2011","journal-title":"Afr. J. Microbiol. Res."},{"key":"B31","first-page":"82","article-title":"Identification of an antifungal chitinase from a potential biocontrol agent, Bacillus cereus 28-9.","volume":"38","author":"Huang","year":"2005","journal-title":"J. Biochem. Mol. Biol."},{"key":"B32","doi-asserted-by":"crossref","first-page":"197","DOI":"10.1016\/S0378-4290(99)00087-8","article-title":"Nitrogen fixation in endophytic and associative symbiosis.","volume":"65","author":"James","year":"2000","journal-title":"Food Crop Res."},{"key":"B33","doi-asserted-by":"publisher","DOI":"10.1016\/j.bcab.2019.101487","article-title":"Microbial biofertilizers: bioresources and eco-friendly technologies for agricultural and environmental sustainability.","volume":"23","author":"Kour","year":"2020","journal-title":"Biocatal. Agric. Biotechnol."},{"key":"B34","doi-asserted-by":"publisher","DOI":"10.3389\/fmicb.2017.00012","article-title":"Plants assemble species specific bacterial communities from common core taxa in three arcto-alpine climate zones.","volume":"8","author":"Kumar","year":"2017","journal-title":"Front. Microbiol."},{"key":"B35","doi-asserted-by":"publisher","first-page":"1547","DOI":"10.1093\/molbev\/msy096","article-title":"MEGA X, molecular evolutionary genetics analysis across computing platforms.","volume":"35","author":"Kumar","year":"2018","journal-title":"Mol. Biol. Evol."},{"key":"B36","doi-asserted-by":"crossref","first-page":"82","DOI":"10.1016\/j.biteb.2018.06.003","article-title":"Production of siderophores by the bacterium Kosakonia radicincitans and its application to control of phytopathogenic fungi.","volume":"3","author":"Lambrese","year":"2018","journal-title":"Bioresour. Technol. Rep."},{"key":"B37","first-page":"209","article-title":"Role of nitrogen for plant growth and development: a review.","volume":"10","author":"Leghari","year":"2016","journal-title":"Adv. Environ. Biol."},{"key":"B38","doi-asserted-by":"publisher","DOI":"10.3389\/fmicb.2017.01268","article-title":"Genetic diversity of nitrogen-fixing and plant growth promoting Pseudomonas species isolated from sugarcane rhizosphere.","volume":"8","author":"Li","year":"2017","journal-title":"Front. Microbiol."},{"key":"B39","doi-asserted-by":"publisher","first-page":"603","DOI":"10.1007\/s12355-016-0480-488","article-title":"Sugar industry and improved sugarcane farming technologies in China.","volume":"18","author":"Li","year":"2016","journal-title":"Sugar Tech."},{"key":"B40","doi-asserted-by":"publisher","first-page":"178","DOI":"10.1111\/j.1472-765X.2011.03088.x","article-title":"A colorimetric assay of 1- aminocyclopropane-1-carboxylate (ACC) based on ninhydrin reaction for rapid screening of bacteria containing ACC deaminase.","volume":"53","author":"Li","year":"2011","journal-title":"Lett. Appl. Microbiol."},{"key":"B41","doi-asserted-by":"publisher","first-page":"391","DOI":"10.1264\/jsme2.me11275","article-title":"Plant growth-promoting nitrogen-fixing enterobacteria are in association with sugarcane plants growing in Guangxi. China.","volume":"27","author":"Lin","year":"2012","journal-title":"Microbes Environ."},{"key":"B42","doi-asserted-by":"crossref","first-page":"402","DOI":"10.1006\/meth.2001.1262","article-title":"Analysis of relative gene expression data using real-time quantitative PCR and the 2-11Ct method.","volume":"25","author":"Livak","year":"2001","journal-title":"Methods"},{"key":"B43","doi-asserted-by":"crossref","first-page":"142","DOI":"10.1111\/j.1399-3054.1948.tb07118.x","article-title":"Production of hydrocyanic acid by bacteria.","volume":"1","author":"Lorck","year":"1948","journal-title":"Physiol. Plant."},{"key":"B44","doi-asserted-by":"crossref","first-page":"1229","DOI":"10.1016\/j.soilbio.2010.04.014","article-title":"Assessment of the plant growth promotion abilities of six bacterial isolates using Zea mays as indicator plant.","volume":"42","author":"Marques","year":"2010","journal-title":"Soil Biol. Biochem."},{"key":"B45","doi-asserted-by":"crossref","first-page":"155","DOI":"10.1007\/s11104-020-04621-1","article-title":"Both the contribution of soil nitrogen and of biological N2 fixation to sugarcane can increase with the inoculation of diazotrophic bacteria.","volume":"454","author":"Martins","year":"2020","journal-title":"Plant Soil."},{"key":"B46","doi-asserted-by":"crossref","first-page":"60","DOI":"10.2174\/22115501113026660038","article-title":"Biotechnological applications of bacterial endophytes.","volume":"3","author":"Mercado-Blanco","year":"2014","journal-title":"Curr. Biotechnol."},{"key":"B47","doi-asserted-by":"publisher","first-page":"979","DOI":"10.1093\/jexbot\/53.370.979","article-title":"The role of glutamine synthetase and glutamate dehydrogenase in nitrogen assimilation and possibilities for improvement in the nitrogen utilization of crops.","volume":"53","author":"Miflin","year":"2002","journal-title":"J. Exp. Bot."},{"key":"B48","first-page":"27","article-title":"Root associated bacteria\u2014friends or enemies? a review.","volume":"38","author":"Mihalache","year":"2015","journal-title":"Mem. Sci. Sect. Rom. Acad."},{"key":"B49","doi-asserted-by":"crossref","first-page":"253","DOI":"10.1007\/s00374-008-0322-2","article-title":"Biological nitrogen fixation in maize (Zea mays L.) by 15N isotope-dilution and identification of associated culturable diazotrophs.","volume":"45","author":"Monta\u00f1ez","year":"2009","journal-title":"Biol Fert Soils."},{"key":"B50","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0099168","article-title":"New insights into 1-Aminocyclopropane1-carboxylate (ACC) deaminase phylogeny. evolution and ecological significance.","volume":"9","author":"Nascimento","year":"2014","journal-title":"PLoS One"},{"key":"B51","doi-asserted-by":"crossref","DOI":"10.1093\/oso\/9780195135848.001.0001","author":"Nei","year":"2000","journal-title":"Molecular Evolution and Phylogenetics."},{"key":"B52","doi-asserted-by":"publisher","first-page":"421","DOI":"10.1099\/mic.0.000643","article-title":"Azotobacter vinelandii: the source of 100 years of discoveries and many more to come.","volume":"164","author":"Noar","year":"2018","journal-title":"Microbiology"},{"key":"B53","doi-asserted-by":"crossref","first-page":"819","DOI":"10.1016\/j.plantsci.2005.05.031","article-title":"Characterization of glutamine synthetase genes in sugarcane genotypes with different rates of biological nitrogen fixation.","volume":"169","author":"Nogueira","year":"2005","journal-title":"Plant Sci."},{"key":"B54","doi-asserted-by":"crossref","DOI":"10.1007\/s11274-017-2364-9","article-title":"Mechanisms of action of plant growth promoting bacteria.","volume":"33","author":"Olanrewaju","year":"2017","journal-title":"World J. Microbiol. Biotechnol."},{"key":"B55","doi-asserted-by":"publisher","DOI":"10.1016\/j.scitotenv.2020.140682","article-title":"Beneficial features of plant growth-promoting rhizobacteria for improving plant growth and health in challenging conditions: a methodical review.","volume":"743","author":"Ole\u0144ska","year":"2020","journal-title":"Sci. Total Enviorn."},{"key":"B56","doi-asserted-by":"crossref","first-page":"831","DOI":"10.1007\/s10658-017-1325-3","article-title":"Biocontrol of Fusarium wilt of Capsicum annuum by rhizospheric bacteria isolated from turmeric endowed with plant growth promotion and disease suppression potential.","volume":"150","author":"Passari","year":"2018","journal-title":"Eur. J. Plant Pathol."},{"key":"B57","doi-asserted-by":"crossref","DOI":"10.1038\/s41598-017-12235-4","article-title":"Insights into the functionality of endophytic actinobacteria with a focus on their biosynthetic potential and secondary metabolites production.","volume":"7","author":"Passari","year":"2017","journal-title":"Sci. Rep."},{"key":"B58","doi-asserted-by":"publisher","first-page":"66","DOI":"10.1016\/0966-842x(93)90036-q","article-title":"Iron acquisition in microbial pathogenesis.","volume":"1","author":"Payne","year":"1993","journal-title":"Trends Microbiol."},{"key":"B59","doi-asserted-by":"publisher","first-page":"10","DOI":"10.1034\/j.1399-3054.2003.00086.x","article-title":"Methods for isolating and characterizing ACC deaminase-containing plant growth-promoting rhizobacteria.","volume":"118","author":"Penrose","year":"2003","journal-title":"Physiol. Plant."},{"key":"B60","doi-asserted-by":"publisher","first-page":"95","DOI":"10.1016\/s0923-2508(00)01172-4","article-title":"Improvement in the RFLP procedure for studying the diversity of nifH genes in communities of nitrogen fixers in soil.","volume":"152","author":"Poly","year":"2001","journal-title":"Res. Microbiol."},{"key":"B61","doi-asserted-by":"publisher","first-page":"5535","DOI":"10.1093\/jxb\/eru320","article-title":"Regulation of amino acid metabolic enzymes and transporters in plants.","volume":"65","author":"Pratelli","year":"2014","journal-title":"J. Exp. Bot."},{"key":"B62","doi-asserted-by":"publisher","first-page":"7511","DOI":"10.1128\/AEM.00836-12","article-title":"Sugarcane growth promotion by the endophytic bacterium Pantoea agglomerans 33.1.","volume":"78","author":"Quecine","year":"2012","journal-title":"Appl. Environ. Microbiol."},{"key":"B63","doi-asserted-by":"publisher","DOI":"10.3389\/fmicb.2019.01106","article-title":"Use of rhizobacteria and mycorrhizae consortium in the open field as a strategy for improving crop nutrition, productivity and soil fertility.","volume":"10","author":"Raklami","year":"2019","journal-title":"Front. Microbiol."},{"key":"B64","doi-asserted-by":"publisher","first-page":"525","DOI":"10.1094\/MPMI.2003.16.6.525","article-title":"Phylogeny of HCN synthase-encoding hcnBC genes in biocontrol fluorescent pseudomonads and its relationship with host plant species and HCN synthesis ability.","volume":"16","author":"Ramette","year":"2003","journal-title":"Mol. Plant Microb. Interact."},{"key":"B65","doi-asserted-by":"publisher","DOI":"10.3389\/fmicb.2020.02044","article-title":"Development and application of low-cost and eco-sustainable bio-stimulant containing a new plant growth-promoting strain Kosakonia pseudosacchari TL13.","volume":"11","author":"Romano","year":"2020","journal-title":"Front. Microbiol."},{"key":"B66","first-page":"406","article-title":"The neighbor-joining method, a new method for reconstructing phylogenetic trees.","volume":"4","author":"Saitou","year":"1987","journal-title":"Mol. Biol. Evol."},{"key":"B67","doi-asserted-by":"publisher","first-page":"47","DOI":"10.1016\/0003-2697(87)90612-9","article-title":"Universal chemical assay for the detection and determination of siderophores.","volume":"160","author":"Schwyn","year":"1987","journal-title":"Anal. Biochem."},{"key":"B68","doi-asserted-by":"crossref","first-page":"787","DOI":"10.1007\/s10658-018-1592-7","article-title":"Isolation and characterization of salt-tolerant bacilli from chickpea (Cicer arietinum L.) rhizosphere for plant growth promotion and biocontrol traits.","volume":"153","author":"Sharma","year":"2019","journal-title":"Eur. J. Plant Pathol."},{"key":"B69","doi-asserted-by":"crossref","DOI":"10.3390\/microorganisms9112203","article-title":"Insights into the bacterial and nitric oxide-induced salt tolerance in sugarcane and their growth promoting abilities.","volume":"9","author":"Sharma","year":"2021","journal-title":"Microorganisms"},{"key":"B70","doi-asserted-by":"publisher","DOI":"10.1038\/srep39266","article-title":"Root transcriptome of two contrasting indica rice cultivars uncovers regulators of root development and physiological responses.","volume":"6","author":"Singh","year":"2016","journal-title":"Sci. Rep."},{"key":"B73","doi-asserted-by":"publisher","DOI":"10.3389\/fmicb.2020.600417","article-title":"Diazotrophic bacteria pantoea dispersa and Enterobacter asburiae promote sugarcane growth by inducing nitrogen uptake and defense-related gene expression.","volume":"11","author":"Singh","year":"","journal-title":"Front. Microbiol."},{"key":"B71","doi-asserted-by":"crossref","first-page":"12","DOI":"10.1080\/17429145.2020.1867770","article-title":"Comparative analysis of protein and differential responses of defense related gene and enzyme activity reveals the long-term molecular responses of sugarcane inoculated with Sporisorium scitamineum.","volume":"16","author":"Singh","year":"","journal-title":"J. Plant Interact."},{"key":"B72","doi-asserted-by":"publisher","DOI":"10.3389\/fmicb.2021.628376","article-title":"Whole genome analysis of sugarcane root-associated endophyte Pseudomonas aeruginosa B18\u2014A plant growth-promoting bacterium with antagonistic potential against Sporisorium scitamineum.","volume":"1","author":"Singh","year":"","journal-title":"Front. Microbiol."},{"key":"B74","doi-asserted-by":"crossref","first-page":"427","DOI":"10.1080\/17429145.2020.1857857","article-title":"Plant-PGPR interaction study of plant growth-promoting diazotrophs Kosakonia radicincitans BA1 and Stenotrophomonas maltophilia COA2 to enhance growth and stress-related gene expression in Saccharum spp.","volume":"15","author":"Singh","year":"","journal-title":"J. Plant Interact."},{"key":"B75","doi-asserted-by":"publisher","DOI":"10.1186\/s12870-020-02400-9","article-title":"Diversity of nitrogen-fixing rhizobacteria associated with sugarcane, a comprehensive study of plant-microbe interactions for growth enhancement in Saccharum spp.","volume":"20","author":"Singh","year":"","journal-title":"BMC Plant Biol."},{"key":"B76","doi-asserted-by":"publisher","first-page":"103","DOI":"10.1007\/978-1-4939-9790-9_10","article-title":"Methods for estimation of nitrogen components in plants and microorganisms.","volume":"2057","author":"Singh","year":"","journal-title":"Methods Mol. Biol."},{"key":"B77","doi-asserted-by":"publisher","DOI":"10.3390\/ijms20030569","article-title":"Proteomic analysis of the resistance mechanisms in sugarcane during Sporisorium scitamineum infection.","volume":"20","author":"Singh","year":"2019","journal-title":"Int. J. Mol. Sci."},{"key":"B78","doi-asserted-by":"crossref","first-page":"631","DOI":"10.1007\/s12355-018-0671-6","article-title":"Physiological and molecular analysis of sugarcane (varieties- F134 and NCo310) during Sporisorium scitamineum interaction.","volume":"21","author":"Singh","year":"2018","journal-title":"Sugar Tech."},{"key":"B79","doi-asserted-by":"crossref","first-page":"91","DOI":"10.1007\/s10725-013-9870-z","article-title":"Multifarious plant growth promoting characteristics of chickpea rhizosphere associated Bacilli help to suppress soil-borne pathogens.","volume":"73","author":"Singh","year":"2014","journal-title":"Plant Growth Regul."},{"key":"B80","doi-asserted-by":"publisher","first-page":"451","DOI":"10.1002\/jobm.201100590","article-title":"Optimization of media components for chitinase production by chickpea rhizosphere associated Lysinibacillus fusiformis B-CM18.","volume":"53","author":"Singh","year":"2013","journal-title":"J. Basic Microbiol."},{"key":"B81","first-page":"8132","article-title":"Effects of inoculation with plant growth promoting rhizobacteria on photosynthesis, antioxidant status and yield of runner bean.","volume":"18","author":"Stefan","year":"2013","journal-title":"Rom. Biotechnol. Lett."},{"key":"B82","doi-asserted-by":"publisher","first-page":"2169","DOI":"10.1105\/tpc.107.052068","article-title":"Multilevel interactions between ethylene and auxin in Arabidopsis roots.","volume":"19","author":"Stepanova","year":"2007","journal-title":"Plant Cell"},{"key":"B83","doi-asserted-by":"publisher","first-page":"959","DOI":"10.1093\/jexbot\/53.370.959","article-title":"Steps towards an integrated view of nitrogen metabolism.","volume":"53","author":"Stitt","year":"2002","journal-title":"J. Exp. Bot."},{"key":"B84","doi-asserted-by":"publisher","first-page":"987","DOI":"10.1128\/AEM.00582-21","article-title":"Potential use of plant colonizing Pantoeaas generic plant growth promoting bacteria for cereal crops.","volume":"41","author":"Suman","year":"2020","journal-title":"J. Environ. Biol."},{"key":"B85","doi-asserted-by":"crossref","first-page":"257","DOI":"10.3923\/biotech.2010.257.273","article-title":"Symbiotic nitrogen fixation-a bioinformatics perspective.","volume":"9","author":"Sur","year":"2010","journal-title":"Biotech"},{"key":"B86","doi-asserted-by":"publisher","DOI":"10.3389\/fmicb.2019.02463","article-title":"Taxogenomics and systematics of the genus pantoea.","volume":"10","author":"Tambong","year":"2019","journal-title":"Front. Microbiol."},{"key":"B87","doi-asserted-by":"crossref","first-page":"435","DOI":"10.1007\/s11104-010-0557-1","article-title":"Two seasons\u2019 study on nifH gene expression and nitrogen fixation by diazotrophic endophytes in sugarcane (Saccharum spp. hybrids): expression of nifH genes similar to those of rhizobia.","volume":"338","author":"Thaweenut","year":"2011","journal-title":"Plant Soil."},{"key":"B88","doi-asserted-by":"publisher","first-page":"671","DOI":"10.1038\/nature01014","article-title":"Agricultural sustainability and intensive production practices.","volume":"418","author":"Tilman","year":"2002","journal-title":"Nature"},{"key":"B89","year":"2020","journal-title":"Sugar: World Markets and Trade."},{"key":"B90","doi-asserted-by":"crossref","first-page":"105","DOI":"10.2136\/sssaj1992.03615995005600010017x","article-title":"Contribution of nitrogen fixation to sugarcane: nitrogen-15 and nitrogen-balance estimates.","volume":"56","author":"Urquiaga","year":"1992","journal-title":"Soil Sci. Soc. Am. J."},{"key":"B91","doi-asserted-by":"publisher","DOI":"10.1016\/j.agee.2019.106583","article-title":"The role of legumes in the sustainable intensification of African smallholder agriculture: lessons learnt and challenges for the future.","volume":"284","author":"Vanlauwe","year":"2019","journal-title":"Agric. Ecosyst. Environ."},{"key":"B92","doi-asserted-by":"publisher","first-page":"577","DOI":"10.1007\/s11103-017-0611-y","article-title":"An integrative overview of the molecular and physiological responses of sugarcane under drought conditions.","volume":"94","author":"Vital","year":"2017","journal-title":"Plant Mol. Biol."},{"key":"B93","doi-asserted-by":"publisher","DOI":"10.1016\/j.scitotenv.2020.141795","article-title":"Global direct nitrous oxide emissions from the bioenergy crop sugarcane (Saccharum spp. inter-specific hybrids).","volume":"752","author":"Yang","year":"2021","journal-title":"Sci. Total Enviorn."}],"container-title":["Frontiers in Microbiology"],"original-title":[],"link":[{"URL":"https:\/\/www.frontiersin.org\/articles\/10.3389\/fmicb.2021.774707\/full","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,11,13]],"date-time":"2023-11-13T23:59:20Z","timestamp":1699919960000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.frontiersin.org\/articles\/10.3389\/fmicb.2021.774707\/full"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2021,12,15]]},"references-count":93,"alternative-id":["10.3389\/fmicb.2021.774707"],"URL":"https:\/\/doi.org\/10.3389\/fmicb.2021.774707","relation":{},"ISSN":["1664-302X"],"issn-type":[{"value":"1664-302X","type":"electronic"}],"subject":[],"published":{"date-parts":[[2021,12,15]]},"article-number":"774707"}}