{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,15]],"date-time":"2026-09-15T02:57:27Z","timestamp":1789441047881,"version":"build-2803163510"},"reference-count":32,"publisher":"Frontiers Media SA","license":[{"start":{"date-parts":[[2021,12,28]],"date-time":"2021-12-28T00:00:00Z","timestamp":1640649600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["frontiersin.org"],"crossmark-restriction":true},"short-container-title":["Front. Microbiol."],"abstract":"<jats:p>This study aimed to investigate the prevalence of fosfomycin <jats:italic>fosA7<\/jats:italic> in <jats:italic>Salmonella enterica<\/jats:italic> isolates from food animals and retail meat products in China and the impact of <jats:italic>fosA7<\/jats:italic> on bacterial fitness. A total of 360 <jats:italic>Salmonella<\/jats:italic> isolates collected from 11 provinces and cities in China were detected for <jats:italic>fosA7<\/jats:italic>. All <jats:italic>fosA7<\/jats:italic>-positive <jats:italic>Salmonella<\/jats:italic> isolates were determined minimum inhibitory concentrations (MICs) and sequenced by Illumina Hiseq. The <jats:italic>fosA7<\/jats:italic> gene of <jats:italic>S<\/jats:italic>. Derby isolate HA2-WA5 was knocked out. The full length of <jats:italic>fosA7<\/jats:italic> was cloned into vector pBR322 and then transformed into various hosts. MICs of fosfomycin, growth curves, stability, and fitness of <jats:italic>fosA7<\/jats:italic> were evaluated. The <jats:italic>fosA7<\/jats:italic> gene was identified in <jats:italic>S<\/jats:italic>. Derby (ST40, <jats:italic>n<\/jats:italic> = 30) and <jats:italic>S<\/jats:italic>. Reading (ST1628, <jats:italic>n<\/jats:italic> = 5). MICs to fosfomycin of 35 <jats:italic>fosA7<\/jats:italic>-positive isolates were 1 to 32 mg\/L. All <jats:italic>fosA7<\/jats:italic> were located on chromosomes of <jats:italic>Salmonella<\/jats:italic>. The deletion of <jats:italic>fosA7<\/jats:italic> in HA2-WA5 decreased fosfomycin MIC by 16-fold and slightly affected its fitness. The acquisition of plasmid-borne <jats:italic>fosA7<\/jats:italic> enhanced MICs of fosfomycin in <jats:italic>Salmonella<\/jats:italic> (1,024-fold) and <jats:italic>Escherichia coli<\/jats:italic> (16-fold). The recombinant plasmid pBR322-<jats:italic>fosA7<\/jats:italic> was stable in <jats:italic>Salmonella<\/jats:italic> Typhimurium, <jats:italic>S<\/jats:italic>. Pullorum, <jats:italic>S<\/jats:italic>. Derby, and <jats:italic>E. coli<\/jats:italic>, except for <jats:italic>Salmonella<\/jats:italic> Enteritidis, and barely affected on the growth of them but significantly increased biological fitness in <jats:italic>Salmonella<\/jats:italic>. The spread of specific <jats:italic>Salmonella<\/jats:italic> serovars such as <jats:italic>S<\/jats:italic>. Derby ST40 will facilitate the dissemination of <jats:italic>fosA7<\/jats:italic>. <jats:italic>fosA7<\/jats:italic> can confer high-level fosfomycin resistance and enhance bacterial fitness in <jats:italic>Salmonella<\/jats:italic> if transferred on plasmids; thus, it has the potential to be a reservoir of the mobilized fosfomycin resistance gene.<\/jats:p>","DOI":"10.3389\/fmicb.2021.781306","type":"journal-article","created":{"date-parts":[[2021,12,28]],"date-time":"2021-12-28T14:34:41Z","timestamp":1640702081000},"update-policy":"https:\/\/doi.org\/10.3389\/crossmark-policy","source":"Crossref","is-referenced-by-count":18,"title":["Chromosomally Located fosA7 in Salmonella Isolates From China"],"prefix":"10.3389","volume":"12","author":[{"given":"Jing","family":"Wang","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yan","family":"Wang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhen-Yu","family":"Wang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Han","family":"Wu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Cai-Yue","family":"Mei","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Peng-Cheng","family":"Shen","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhi-Ming","family":"Pan","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xinan","family":"Jiao","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1965","published-online":{"date-parts":[[2021,12,28]]},"reference":[{"key":"B1","doi-asserted-by":"publisher","first-page":"260","DOI":"10.1038\/nrmicro2319","article-title":"Antibiotic resistance and its cost: is it possible to reverse resistance?","volume":"8","author":"Andersson","year":"2010","journal-title":"Nat. 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