{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,30]],"date-time":"2026-06-30T10:46:27Z","timestamp":1782816387700,"version":"3.54.5"},"reference-count":47,"publisher":"Frontiers Media SA","license":[{"start":{"date-parts":[[2021,12,14]],"date-time":"2021-12-14T00:00:00Z","timestamp":1639440000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["82173526"],"award-info":[{"award-number":["82173526"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["81673199"],"award-info":[{"award-number":["81673199"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100017596","name":"Natural Science Basic Research Program of Shaanxi Province","doi-asserted-by":"publisher","award":["2019JM-445"],"award-info":[{"award-number":["2019JM-445"]}],"id":[{"id":"10.13039\/501100017596","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["frontiersin.org"],"crossmark-restriction":true},"short-container-title":["Front. Microbiol."],"abstract":"<jats:p>To evaluate the probiotic characteristics and safety of <jats:italic>Enterococcus durans<\/jats:italic> isolate A8-1 from a fecal sample of a healthy Chinese infant, we determined the tolerance to low pH, survival in bile salts and NaCl, adhesion ability, biofilm formation, antimicrobial activity, toxin gene distribution, hemolysis, gelatinase activity, antibiotic resistance, and virulence to <jats:italic>Galleria mellonella<\/jats:italic> and interpreted the characters by genome resequencing. Phenotypically, <jats:italic>E. durans<\/jats:italic> A8-1 survived at pH 5.0 in 7.0% NaCl and 3% bile salt under aerobic and anaerobic condition. The bacterium had higher adhesion ability toward mucin, collagen, and Bovine Serum Albumin (BSA) <jats:italic>in vitro<\/jats:italic> and showed high hydrophobicity (79.2% in chloroform, 49.2% in xylene), auto-aggregation activity (51.7%), and could co-aggregate (66.2%) with <jats:italic>Salmonella typhimurium<\/jats:italic>. It had adhesion capability to intestinal epithelial Caco-2 cells (38.74%) with moderate biofilm production and antimicrobial activity against several Gram-positive pathogenic bacteria. A8-1 can antagonize the adhesion of <jats:italic>S. typhimurium<\/jats:italic> ATCC14028 on Caco-2 cells to protect the integrity of the cell membrane by detection of lactate dehydrogenase (LDH) and AKP activities. A8-1 also helps the cell relieve the inflammation induced by lipopolysaccharide by reducing the expression of cytokine IL-8 (<jats:italic>P<\/jats:italic> = 0.002) and TNF-\u03b1 (<jats:italic>P<\/jats:italic> &amp;gt; 0.05), and increasing the IL-10 (<jats:italic>P<\/jats:italic> &amp;lt; 0.001). For the safety evaluation, A8-1 showed no hemolytic activity, no gelatinase activity, and had only <jats:italic>asa1<\/jats:italic> positive in the seven detected virulence genes in polymerase chain reaction (PCR), whereas it was not predicted in the genome sequence. It was susceptible to benzylpenicillin, ampicillin, ciprofloxacin, levofloxacin, moxifloxacin, tigecycline, nitrofurantoin, linezolid, vancomycin, erythromycin, and quinupristin\/dalofopine except clindamycin, which was verified by the predicted <jats:italic>lasA<\/jats:italic>, <jats:italic>lmrB<\/jats:italic>, <jats:italic>lmrC<\/jats:italic>, and <jats:italic>lmrD<\/jats:italic> genes contributing to the clindamycin resistance. The virulence test of <jats:italic>G. mellonella<\/jats:italic> showed that it had toxicity lower than 10% at 1 \u00d7 10<jats:sup>7<\/jats:sup> CFU. According to the results of these evaluated attributes, <jats:italic>E. durans<\/jats:italic> strain A8-1 could be a promising probiotic candidate for applications.<\/jats:p>","DOI":"10.3389\/fmicb.2021.799173","type":"journal-article","created":{"date-parts":[[2021,12,14]],"date-time":"2021-12-14T11:23:13Z","timestamp":1639480993000},"update-policy":"https:\/\/doi.org\/10.3389\/crossmark-policy","source":"Crossref","is-referenced-by-count":20,"title":["Probiotic Potential Analysis and Safety Evaluation of Enterococcus durans A8-1 Isolated From a Healthy Chinese Infant"],"prefix":"10.3389","volume":"12","author":[{"given":"Yi","family":"Zhou","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lu","family":"Shi","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Juan","family":"Wang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jia","family":"Yuan","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jin","family":"Liu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lijuan","family":"Liu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rong","family":"Da","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yue","family":"Cheng","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bei","family":"Han","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1965","published-online":{"date-parts":[[2021,12,14]]},"reference":[{"key":"B1","doi-asserted-by":"publisher","first-page":"188","DOI":"10.1186\/s12934-019-1239-1","article-title":"Isolation, identification, and potential probiotic characterization of isolated lactic acid bacteria and in vitro investigation of the cytotoxicity, antioxidant, and antidiabetic activities in fermented sausage.","volume":"18","author":"AlKalbani","year":"2019","journal-title":"Microb. 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