{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,28]],"date-time":"2026-08-28T16:57:05Z","timestamp":1787936225778,"version":"build-2784847793"},"reference-count":52,"publisher":"Frontiers Media SA","license":[{"start":{"date-parts":[[2022,1,5]],"date-time":"2022-01-05T00:00:00Z","timestamp":1641340800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001349","name":"National Medical Research Council","doi-asserted-by":"publisher","award":["OF-YIRG NMRC\/OFYIRG\/0070\/2018"],"award-info":[{"award-number":["OF-YIRG NMRC\/OFYIRG\/0070\/2018"]}],"id":[{"id":"10.13039\/501100001349","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001348","name":"Agency for Science, Technology and Research","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100001348","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001348","name":"Agency for Science, Technology and Research","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100001348","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001459","name":"Ministry of Education - Singapore","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100001459","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001352","name":"National University of Singapore","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100001352","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["frontiersin.org"],"crossmark-restriction":true},"short-container-title":["Front. Microbiol."],"abstract":"<jats:p><jats:italic>Plasmodium knowlesi<\/jats:italic> is responsible for zoonotic malaria infections that are potentially fatal. While the severe pathology of falciparum malaria is associated with cytoadherence phenomena by <jats:italic>Plasmodium falciparum<\/jats:italic>-infected erythrocytes (IRBC), information regarding cytoadherence properties of <jats:italic>P. knowlesi<\/jats:italic>-IRBC remained scarce. Here, we characterized the cytoadherence properties of RBC infected with the laboratory-adapted <jats:italic>P. knowlesi<\/jats:italic> A1-H.1 strain. We found that late-stage IRBC formed rosettes in a human serum-dependent manner, and rosettes hampered IRBC phagocytosis. IRBC did not adhere much to unexposed (unstimulated) human endothelial cell lines derived from the brain (hCMEC\/D3), lungs (HPMEC), and kidneys (HRGEC). However, after being \u201cprimed\u201d with <jats:italic>P. knowlesi<\/jats:italic> culture supernatant, the IRBC-endothelial cytoadherence rate increased in HPMEC and HRGEC, but not in hCMEC\/D3 cells. Both endothelial cytoadherence and rosetting phenomena were abrogated by treatment of <jats:italic>P. knowlesi<\/jats:italic>-IRBC with trypsin. We also found that different receptors were involved in IRBC cytoadherence to different types of endothelial cells. Although some of the host receptors were shared by both <jats:italic>P. falciparum-<\/jats:italic> and <jats:italic>P. knowlesi-<\/jats:italic>IRBC, the availability of glycoconjugates on the receptors might influence the capacity of <jats:italic>P. knowlesi<\/jats:italic>-IRBC to cytoadhere to these receptors.<\/jats:p>","DOI":"10.3389\/fmicb.2021.804417","type":"journal-article","created":{"date-parts":[[2022,1,5]],"date-time":"2022-01-05T08:17:32Z","timestamp":1641370652000},"update-policy":"https:\/\/doi.org\/10.3389\/crossmark-policy","source":"Crossref","is-referenced-by-count":14,"title":["Cytoadherence Properties of Plasmodium knowlesi-Infected Erythrocytes"],"prefix":"10.3389","volume":"12","author":[{"given":"Wenn-Chyau","family":"Lee","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shahhaziq","family":"Shahari","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Samantha Yee Teng","family":"Nguee","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yee-Ling","family":"Lau","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Laurent","family":"R\u00e9nia","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1965","published-online":{"date-parts":[[2022,1,5]]},"reference":[{"key":"B1","doi-asserted-by":"publisher","DOI":"10.1038\/s41598-020-73713-w","article-title":"Rosettes integrity protects Plasmodium vivax of being phagocytized.","volume":"10","author":"Albrecht","year":"2020","journal-title":"Sci. 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