{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,7,30]],"date-time":"2025-07-30T16:03:51Z","timestamp":1753891431687,"version":"3.41.2"},"reference-count":41,"publisher":"Frontiers Media SA","license":[{"start":{"date-parts":[[2022,10,5]],"date-time":"2022-10-05T00:00:00Z","timestamp":1664928000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["frontiersin.org"],"crossmark-restriction":true},"short-container-title":["Front. Microbiol."],"abstract":"<jats:p>Systemic candidiasis is the fourth leading cause of healthcare-associated infections worldwide. The combination therapy based on existing antifungal agents is well-established to overcome drug resistance and restore antifungal efficacy against drug-resistant strains. In this study, a simple and sensitive liquid chromatography with tandem mass spectrometry (LC-MS\/MS) method was developed to quantify the intracellular fluconazole (FLC) content in the opportunistic human fungal pathogen <jats:italic>Candida albicans<\/jats:italic>. The cell lysates were prepared by lysing <jats:italic>C. albicans<\/jats:italic> cells with Precellys homogenizers and FLC was extracted with methylene chloride. The entire extraction approach was simple, precise and reliable. The extracts were separated on a Zorbax SB-C18 column using a mobile phase of acetonitrile (solvent A) and deionized water plus 0.1% formic acid. FLC and ketoconazole (KCZ, internal standard) were monitored in positive mode using electrospray ionization source. The multiple reaction monitoring transitions (precursor to product) were monitored for FLC m\/z 307.1 \u2192 238.2 and for the internal standard KCZ m\/z 531.2 \u2192 489.1. The linear for this method were in the range from 5.0 to 1000.0 ng\/mL. The precision and accuracy of the samples were relative standard deviations (RSD) &amp;lt; 1.0% for intra-day and RSD &amp;lt; 0.51% for inter-day. The overall recovery of FLC from samples was higher than 77.61%. Furthermore, this method was successfully applied and validated in 36 clinical isolated strains. Taken together, we established a highly accurate, efficient, and reproducible method for quantifying the intracellular content of FLC in <jats:italic>C. albicans<\/jats:italic>.<\/jats:p>","DOI":"10.3389\/fmicb.2022.1007576","type":"journal-article","created":{"date-parts":[[2022,10,5]],"date-time":"2022-10-05T07:38:01Z","timestamp":1664955481000},"update-policy":"https:\/\/doi.org\/10.3389\/crossmark-policy","source":"Crossref","is-referenced-by-count":0,"title":["Development and validation of a sensitive LC-MS\/MS method for determination of intracellular concentration of fluconazole in Candida albicans"],"prefix":"10.3389","volume":"13","author":[{"given":"Xiaofei","family":"Wang","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xiaojuan","family":"Wang","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Tongkai","family":"Cai","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yulin","family":"Qin","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ling","family":"Li","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yuanying","family":"Jiang","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Bing","family":"Han","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yongbing","family":"Cao","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1965","published-online":{"date-parts":[[2022,10,5]]},"reference":[{"key":"B1","doi-asserted-by":"publisher","first-page":"2043","DOI":"10.1007\/s00216-012-6272-4","article-title":"Anidulafungin\u2013challenges in development and validation of an LC-MS\/MS bioanalytical method validated for regulated clinical studies.","volume":"404","author":"Alebic-Kolbah","year":"2012","journal-title":"Anal. 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