{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,23]],"date-time":"2026-01-23T10:02:41Z","timestamp":1769162561728,"version":"3.49.0"},"reference-count":57,"publisher":"Frontiers Media SA","license":[{"start":{"date-parts":[[2022,10,13]],"date-time":"2022-10-13T00:00:00Z","timestamp":1665619200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100004306","name":"Shandong Agricultural University","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100004306","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["frontiersin.org"],"crossmark-restriction":true},"short-container-title":["Front. Microbiol."],"abstract":"<jats:p>This study aimed to investigate the effect of dietary supplementation with Bopu powder on intestinal development and bacterial community composition in broiler chickens. A total of 486 1-day-old arbor acres broilers were fed a basal diet (CON group), a basal diet supplemented with 50\u2009mg\/kg aureomycin (AB group), or a basal diet supplemented with 40\u2009mg\/kg Bopu powder (BP group). The results showed that the BP group had significantly lower serum tumor necrosis factor-\u03b1 (TNF-\u03b1), interleukin (IL)-1\u03b2, IL-6, and diamine oxidase concentrations and had significantly higher serum IL-10 concentrations than CON group (<jats:italic>p<\/jats:italic>\u2009&amp;lt;\u20090.05). Groups AB and BP had a significantly higher weight per unit length of the small intestine and villus height than the CON group (<jats:italic>p<\/jats:italic>\u2009&amp;lt;\u20090.05), and BP group had a significantly higher ratio of villus height to crypt depth than groups CON and AB (<jats:italic>p<\/jats:italic>\u2009&amp;lt;\u20090.05). Compared to the CON group, dietary Bopu powder or aureomycin supplementation significantly increased transforming growth factor-\u03b1 concentration and mRNA expressions of zonula occludens-1 (ZO-1) and occludin, and decreased intestinal mucosal concentrations of TNF-\u03b1, IL-6, IL-10, caspase-3, and caspase-8 and mRNA expressions of nuclear factor-kappa-B and <jats:italic>Bax<\/jats:italic>\/<jats:italic>Bcl-2<\/jats:italic> ratio in the intestinal mucosa (<jats:italic>p<\/jats:italic>\u2009&amp;lt;\u20090.05). Meanwhile, BP group had significantly higher ZO-1, secretory immunoglobulin A, interferon-\u03b3 concentrations, and mRNA expressions of glucose transporter type-2 and sirtuin-1, and significantly lower IL-1\u03b2 concentration than groups CON and AB in intestinal mucosa (<jats:italic>p<\/jats:italic>\u2009&amp;lt;\u20090.05). Dietary Bopu powder supplementation significantly increased the concentration of trefoil factor family member and mRNA expressions of superoxide dismutase-1 and bcl-2 associated X, and significantly reduced casepase-9 concentration and myeloid differentiation primary response-88 expression in the intestinal mucosa of broiler chickens relative to CON group (<jats:italic>p<\/jats:italic>\u2009&amp;lt;\u20090.05). Moreover, results of high-throughput sequencing showed that broilers in the BP group had microbial community structure distinct from that in CON group, and the addition of Bopu powder increased the abundances of <jats:italic>Faecalibacterium<\/jats:italic> and <jats:italic>Colidextribacter<\/jats:italic> (<jats:italic>p<\/jats:italic>\u2009&amp;lt;\u20090.05). Therefore, our study suggests a synergic response of intestinal development and microbiota to the Bopu powder, and provides a theoretical basis as a potential substitute for antibiotics.<\/jats:p>","DOI":"10.3389\/fmicb.2022.1019130","type":"journal-article","created":{"date-parts":[[2022,10,13]],"date-time":"2022-10-13T06:22:17Z","timestamp":1665642137000},"update-policy":"https:\/\/doi.org\/10.3389\/crossmark-policy","source":"Crossref","is-referenced-by-count":15,"title":["Effects of dietary Bopu powder supplementation on intestinal development and microbiota in broiler chickens"],"prefix":"10.3389","volume":"13","author":[{"given":"Yang","family":"Liu","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Qinjin","family":"Wang","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hua","family":"Liu","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jiax\u2011ing","family":"Niu","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ning","family":"Jiao","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Libo","family":"Huang","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Shuzhen","family":"Jiang","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Qinglin","family":"Guan","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Weiren","family":"Yang","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yang","family":"Li","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1965","published-online":{"date-parts":[[2022,10,13]]},"reference":[{"key":"ref1","first-page":"1","volume-title":"Broiler Management Guide","author":"Acres","year":"2009"},{"key":"ref2","doi-asserted-by":"publisher","first-page":"984","DOI":"10.1016\/j.jaci.2016.06.033","article-title":"Interleukins (from IL-1 to IL-38), interferons, transforming growth factor \u03b2, and TNF-\u03b1: receptors, functions, and roles in diseases","volume":"138","author":"Akdis","year":"2016","journal-title":"J. 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