{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,15]],"date-time":"2026-07-15T14:52:14Z","timestamp":1784127134822,"version":"3.55.0"},"reference-count":32,"publisher":"Frontiers Media SA","license":[{"start":{"date-parts":[[2022,11,10]],"date-time":"2022-11-10T00:00:00Z","timestamp":1668038400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100012166","name":"National Key Research and Development Program of China","doi-asserted-by":"publisher","award":["2021YFC2301704"],"award-info":[{"award-number":["2021YFC2301704"]}],"id":[{"id":"10.13039\/501100012166","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["frontiersin.org"],"crossmark-restriction":true},"short-container-title":["Front. Microbiol."],"abstract":"<jats:p>Getah virus (GETV) disease is a mosquito-borne infectious disease that causes fever, aseptic meningitis, and abortion in a variety of animals. Currently, the epidemic trend of GETV disease increases seriously worldwide, especially in China, posing a potential threat to animal safety and public health. However, there are few reports about the epidemiological investigation of GETV disease in China as well as a lack of commercial diagnostic kit for GETV antibody. Therefore, the establishment of a rapid, sensitive and suitable GETV antibody detection method for large-scale samples is an urgent request to fully understand the prevalence of GETV disease. Here, a recombinant plasmid pET22b-GETV-E2d that contained the domain of GETV-E2 (E2d) fused to His-tag was constructed to express recombinant protein E2d (rE2d) in <jats:italic>Escherichia coli<\/jats:italic>. The rE2d was mainly expressed in inclusion bodies. And it was purified successfully by nickel affinity column so that it could be used to develop an indirect ELISA (rE2d-ELISA). After optimizing reaction conditions of rE2d-ELISA, the cut-off value was determined as 0.396 with 100 equine sera tested by virus neutralization test (VNT). Furthermore, rE2d-ELISA method showed the positive rate of IgG antibodies against GETV was 54.3% based on testing 646 clinical serum samples obtained in Xinjiang whereas the overall coincidence rate between rE2d-ELISA and VNT was 94.0%, with 98.2% sensitivity and 92.6% specificity. The findings suggest that the developed IgG ELISA employing recombinant E2d promises was an efficient and low-cost type of antibody detection method for horse, which will benefit for prevention of GETV outbreaks in horses.<\/jats:p>","DOI":"10.3389\/fmicb.2022.1029444","type":"journal-article","created":{"date-parts":[[2022,11,10]],"date-time":"2022-11-10T08:49:58Z","timestamp":1668070198000},"update-policy":"https:\/\/doi.org\/10.3389\/crossmark-policy","source":"Crossref","is-referenced-by-count":12,"title":["Development and application of an indirect ELISA for detecting equine IgG antibodies against Getah virus with recombinant E2 domain protein"],"prefix":"10.3389","volume":"13","author":[{"given":"Xiangshu","family":"Qiu","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xinyu","family":"Cao","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ning","family":"Shi","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"He","family":"Zhang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiangyu","family":"Zhu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yan","family":"Gao","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhanhai","family":"Mai","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ningyi","family":"Jin","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Huijun","family":"Lu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1965","published-online":{"date-parts":[[2022,11,10]]},"reference":[{"key":"ref1","doi-asserted-by":"publisher","first-page":"2286","DOI":"10.1128\/jcm.00550-15","article-title":"Epizootiological investigation of Getah virus infection among racehorses in Japan in 2014","volume":"53","author":"Bannai","year":"2015","journal-title":"J. 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Public Health"},{"key":"ref19","doi-asserted-by":"publisher","first-page":"243","DOI":"10.1186\/s12917-018-1570-5","article-title":"Development and application of an indirect ELISA for the detection of antibodies to porcine epidemic diarrhea virus based on a recombinant spike protein","volume":"14","author":"Lin","year":"2018","journal-title":"BMC Vet. Res."},{"key":"ref20","doi-asserted-by":"publisher","first-page":"741","DOI":"10.1089\/vbz.2018.2434","article-title":"Rapid visual detection of Getah virus using a loop-mediated isothermal amplification method","volume":"19","author":"Liu","year":"2019","journal-title":"Vector Borne Zoonotic Dis."},{"key":"ref21","doi-asserted-by":"publisher","first-page":"350","DOI":"10.1016\/j.jinf.2019.11.016","article-title":"Getah virus: An increasing threat in China","volume":"80","author":"Lu","year":"2020","journal-title":"J. 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Dis."},{"key":"ref28","doi-asserted-by":"publisher","first-page":"527","DOI":"10.1136\/vr.134.20.527","article-title":"Serological evidence for the transmission of Getah virus in Hong Kong","volume":"134","author":"Shortridge","year":"1994","journal-title":"Vet. Rec."},{"key":"ref29","doi-asserted-by":"publisher","first-page":"297","DOI":"10.1515\/cclm-2013-0363","article-title":"Analysis of antibody response (IgM, IgG, IgG3) to chikungunya virus using panel of peptides derived from envelope protein for serodiagnosis","volume":"52","author":"Verma","year":"2014","journal-title":"Clin. Chem. Lab. 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Virol."}],"container-title":["Frontiers in Microbiology"],"original-title":[],"link":[{"URL":"https:\/\/www.frontiersin.org\/articles\/10.3389\/fmicb.2022.1029444\/full","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2022,11,10]],"date-time":"2022-11-10T08:50:03Z","timestamp":1668070203000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.frontiersin.org\/articles\/10.3389\/fmicb.2022.1029444\/full"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2022,11,10]]},"references-count":32,"alternative-id":["10.3389\/fmicb.2022.1029444"],"URL":"https:\/\/doi.org\/10.3389\/fmicb.2022.1029444","relation":{},"ISSN":["1664-302X"],"issn-type":[{"value":"1664-302X","type":"electronic"}],"subject":[],"published":{"date-parts":[[2022,11,10]]},"article-number":"1029444"}}