{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,21]],"date-time":"2026-03-21T09:17:15Z","timestamp":1774084635569,"version":"3.50.1"},"reference-count":47,"publisher":"Frontiers Media SA","license":[{"start":{"date-parts":[[2022,11,1]],"date-time":"2022-11-01T00:00:00Z","timestamp":1667260800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["frontiersin.org"],"crossmark-restriction":true},"short-container-title":["Front. Microbiol."],"abstract":"<jats:p>In this study, we characterized a novel chromosome-encoded aminoglycoside nucleotidyltransferase (ANT), AadA36, from the <jats:italic>Providencia stuartii<\/jats:italic> strain P14 isolated from the sputum specimen of a burn patient at a hospital in Wenzhou, China. Among the functionally characterized ANTs, AadA36 shared the highest amino acid sequence identity of 51.91% with AadA14. The whole genome of <jats:italic>P. stuartii<\/jats:italic> P14 consisted of one chromosome and two plasmids (designated pP14-166 and pP14-114). A total of 19 genes with \u226580% similarity with functionally characterized antimicrobial resistance genes (ARGs) were identified in the whole genome, including aminoglycosides [<jats:italic>aac(2\u2032)-Ia<\/jats:italic>, <jats:italic>aph(6)-Id<\/jats:italic>, <jats:italic>aph(3\u2033)-Ib<\/jats:italic>, <jats:italic>aac(6\u2032)-Ib<\/jats:italic>, <jats:italic>ant(3\u2033)-IIa<\/jats:italic>, <jats:italic>aph(3\u2032)-Ia<\/jats:italic>], \u03b2-lactams (<jats:italic>bla<\/jats:italic><jats:sub>CMY-2<\/jats:sub> and <jats:italic>bla<\/jats:italic><jats:sub>OXA-10<\/jats:sub>) and so on. Antimicrobial susceptibility testing showed that the <jats:italic>aadA36<\/jats:italic> gene conferred specific resistance to spectinomycin and streptomycin, and the minimum inhibitory concentration (MIC) of these antimicrobials increased 128- and 64-fold compared with the control strain. The kinetic parameters of AadA36 were consistent with the MIC data of spectinomycin and streptomycin, with <jats:italic>k<jats:sub>cat<\/jats:sub><\/jats:italic>\/<jats:italic>K<jats:sub>m<\/jats:sub><\/jats:italic> ratios of (1.07\u2009\u00b1\u20092.23)\u2009\u00d7\u200910<jats:sup>4<\/jats:sup>\u2009M<jats:sup>\u22121<\/jats:sup> s<jats:sup>\u22121<\/jats:sup> and (8.96\u2009\u00b1\u20091.01)\u2009\u00d7\u200910<jats:sup>3<\/jats:sup> M<jats:sup>\u22121<\/jats:sup> s<jats:sup>\u22121<\/jats:sup>, respectively. The identification of a novel aminoglycoside resistance gene will help us further understand the complexity of the resistance mechanisms and provide deep insights into the dissemination of resistance genes in the microbial population.<\/jats:p>","DOI":"10.3389\/fmicb.2022.1035651","type":"journal-article","created":{"date-parts":[[2022,11,1]],"date-time":"2022-11-01T05:24:00Z","timestamp":1667280240000},"update-policy":"https:\/\/doi.org\/10.3389\/crossmark-policy","source":"Crossref","is-referenced-by-count":8,"title":["AadA36, a novel chromosomal aminoglycoside nucleotidyltransferase from a clinical isolate of Providencia stuartii"],"prefix":"10.3389","volume":"13","author":[{"given":"Mengdi","family":"Gao","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Chunlin","family":"Feng","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yongan","family":"Ji","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yaokai","family":"Shi","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Weina","family":"Shi","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Lei","family":"Zhang","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Shuang","family":"Liu","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Anqi","family":"Li","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xueya","family":"Zhang","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Qiaoling","family":"Li","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Junwan","family":"Lu","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Qiyu","family":"Bao","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hailin","family":"Zhang","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1965","published-online":{"date-parts":[[2022,11,1]]},"reference":[{"key":"ref1","doi-asserted-by":"publisher","first-page":"3052","DOI":"10.1128\/AAC.00304-10","article-title":"Two novel antibiotic resistance genes, tet(44) and ant(6)-Ib, are located within a transferable pathogenicity island in campylobacter fetus subsp. fetus","volume":"54","author":"Abril","year":"2010","journal-title":"Antimicrob. 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