{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,22]],"date-time":"2026-04-22T23:24:58Z","timestamp":1776900298228,"version":"3.51.2"},"reference-count":51,"publisher":"Frontiers Media SA","license":[{"start":{"date-parts":[[2022,10,31]],"date-time":"2022-10-31T00:00:00Z","timestamp":1667174400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100002923","name":"CONICET","doi-asserted-by":"publisher","award":["PUE-0086"],"award-info":[{"award-number":["PUE-0086"]}],"id":[{"id":"10.13039\/501100002923","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100002923","name":"CONICET","doi-asserted-by":"publisher","award":["PIP-11220200102517CO"],"award-info":[{"award-number":["PIP-11220200102517CO"]}],"id":[{"id":"10.13039\/501100002923","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["frontiersin.org"],"crossmark-restriction":true},"short-container-title":["Front. Microbiol."],"abstract":"<jats:p><jats:italic>Brucella<\/jats:italic> spp. are the etiological agent of animal and human brucellosis. We have reported previously that cyclophilins of <jats:italic>Brucella<\/jats:italic> (CypA and CypB) are upregulated within the intraphagosomal replicative niche and required for stress adaptation and host intracellular survival and virulence. Here, we characterize <jats:italic>B. abortus<\/jats:italic> cyclophilins, CypA, and CypB from a biochemical standpoint by studying their PPIase activity, chaperone activity, and oligomer formation. Even though CypA and CypB are very similar in sequence and share identical chaperone and PPIase activities, we were able to identify outstanding differential features between them. A series of differential peptide loops were predicted when comparing CypA and CypB, differences that might explain why specific antibodies (anti-CypA or anti-CypB) were able to discriminate between both cyclophilins without cross-reactivity. In addition, we identified the presence of critical amino acids in CypB, such as the Trp<jats:sup>134<\/jats:sup> which is responsible for the cyclosporin A inhibition, and the Cys<jats:sup>128<\/jats:sup> that leads to CypB homodimer formation by establishing a disulfide bond. Here, we demonstrated that CypB dimer formation was fully required for stress adaptation, survival within HeLa cells, and mouse infection in <jats:italic>B. abortus<\/jats:italic>. The presence of Trp<jats:sup>134<\/jats:sup> and the Cys<jats:sup>128<\/jats:sup> in CypB, which are not present in CypA, suggested that two different kinds of cyclophilins have evolved in <jats:italic>Brucella<\/jats:italic>, one with eukaryotic features (CypB), another (CypA) with similar features to Gram-negative cyclophilins.<\/jats:p>","DOI":"10.3389\/fmicb.2022.1046640","type":"journal-article","created":{"date-parts":[[2022,10,31]],"date-time":"2022-10-31T04:22:54Z","timestamp":1667190174000},"update-policy":"https:\/\/doi.org\/10.3389\/crossmark-policy","source":"Crossref","is-referenced-by-count":4,"title":["Biochemical and functional characterization of Brucella abortus cyclophilins: So similar, yet so different"],"prefix":"10.3389","volume":"13","author":[{"given":"Emanuel J.","family":"Muruaga","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Gabriel","family":"Briones","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mara S.","family":"Roset","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1965","published-online":{"date-parts":[[2022,10,31]]},"reference":[{"key":"ref1","doi-asserted-by":"publisher","first-page":"476","DOI":"10.1016\/j.jmb.2007.07.071","article-title":"The rough energy landscape of superfolder GFP is linked to the chromophore","volume":"373","author":"Andrews","year":"2007","journal-title":"J. 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Pathol."},{"key":"ref8","doi-asserted-by":"publisher","first-page":"164","DOI":"10.1007\/s11274-017-2330-6","article-title":"Microbial cyclophilins: specialized functions in virulence and beyond","volume":"33","author":"Dimou","year":"2017","journal-title":"World J. Microbiol. Biotechnol."},{"key":"ref9","doi-asserted-by":"publisher","first-page":"176","DOI":"10.1159\/000329486","article-title":"Gene expression and biochemical characterization of Azotobacter vinelandii cyclophilins and protein interaction studies of the cytoplasmic isoform with dnaK and lpxH","volume":"20","author":"Dimou","year":"2011","journal-title":"J. Mol. Microbiol. Biotechnol."},{"key":"ref10","doi-asserted-by":"publisher","first-page":"7347","DOI":"10.1073\/pnas.77.12.7347","article-title":"Broad host range DNA cloning system for gram-negative bacteria: construction of a gene bank of rhizobium meliloti","volume":"77","author":"Ditta","year":"1980","journal-title":"Proc. Natl. Acad. Sci. U. S. A."},{"key":"ref11","doi-asserted-by":"publisher","first-page":"258","DOI":"10.1006\/jmbi.1997.1151","article-title":"Crystal structure of cytoplasmic Escherichia coli peptidyl-prolyl isomerase: evidence for decreased mobility of loops upon complexation","volume":"271","author":"Edwards","year":"1997","journal-title":"J. Mol. Biol."},{"key":"ref12","doi-asserted-by":"publisher","first-page":"3453","DOI":"10.2741\/1494","article-title":"Insights into the catalytic mechanism of peptidyl prolyl cis\/trans isomerases","volume":"9","author":"Fanghanel","year":"2004","journal-title":"Front. Biosci."},{"key":"ref13","doi-asserted-by":"publisher","first-page":"4839","DOI":"10.1007\/s00253-019-09795-y","article-title":"Exploring lectin-like activity of the S-layer protein of Lactobacillus acidophilus ATCC 4356","volume":"103","author":"Fina Martin","year":"2019","journal-title":"Appl. Microbiol. 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Chem."},{"key":"ref17","doi-asserted-by":"publisher","first-page":"377","DOI":"10.1016\/S0969-2126(01)00599-8","article-title":"Activation of the redox-regulated molecular chaperone Hsp33--a two-step mechanism","volume":"9","author":"Graumann","year":"2001","journal-title":"Structure"},{"key":"ref18","doi-asserted-by":"publisher","first-page":"968","DOI":"10.1074\/jbc.M510014200","article-title":"The streptococcal lipoprotein rotamase a (SlrA) is a functional peptidyl-prolyl isomerase involved in pneumococcal colonization","volume":"281","author":"Hermans","year":"2006","journal-title":"J. Biol. Chem."},{"key":"ref19","doi-asserted-by":"publisher","first-page":"6557","DOI":"10.1128\/jb.172.11.6557-6567.1990","article-title":"Transposon vectors containing non-antibiotic resistance selection markers for cloning and stable chromosomal insertion of foreign genes in gram-negative bacteria","volume":"172","author":"Herrero","year":"1990","journal-title":"J. Bacteriol."},{"key":"ref20","doi-asserted-by":"publisher","first-page":"e0157070","DOI":"10.1371\/journal.pone.0157070","article-title":"Structural and functional characterization of a novel family of Cyclophilins, the AquaCyps","volume":"11","author":"Jakob","year":"2016","journal-title":"PLoS One"},{"key":"ref21","doi-asserted-by":"publisher","first-page":"539","DOI":"10.1016\/0022-2836(92)90841-7","article-title":"Similarities and differences between human cyclophilin A and other beta-barrel structures. Structural refinement at 1.63 A resolution","volume":"228","author":"Ke","year":"1992","journal-title":"J. Mol. Biol."},{"key":"ref22","doi-asserted-by":"publisher","first-page":"72","DOI":"10.3389\/fcimb.2015.00072","article-title":"Type IV secretion system of Brucella spp. and its effectors","volume":"5","author":"Ke","year":"2015","journal-title":"Front. Cell. Infect. Microbiol."},{"key":"ref23","doi-asserted-by":"publisher","first-page":"9483","DOI":"10.1073\/pnas.88.21.9483","article-title":"Crystal structure of recombinant human T-cell cyclophilin A at 2.5 a resolution","volume":"88","author":"Ke","year":"1991","journal-title":"Proc. Natl. Acad. Sci. U. S. A."},{"key":"ref24","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.00379-18","article-title":"The intracellular Cyclophilin PpiB contributes to the virulence of Staphylococcus aureus independently of its peptidyl-prolyl cis\/trans isomerase activity","volume":"86","author":"Keogh","year":"2018","journal-title":"Infect. Immun."},{"key":"ref25","doi-asserted-by":"publisher","first-page":"4389","DOI":"10.1128\/IAI.71.8.4389-4397.2003","article-title":"Biochemical and functional analyses of the Mip protein: influence of the N-terminal half and of peptidylprolyl isomerase activity on the virulence of legionella pneumophila","volume":"71","author":"Kohler","year":"2003","journal-title":"Infect. Immun."},{"key":"ref26","doi-asserted-by":"publisher","first-page":"e202100490","DOI":"10.1002\/cbic.202100490","article-title":"Controlling enzymatic activity by modulating the Oligomerization state via chemical rescue and optical control","volume":"23","author":"Kropp","year":"2022","journal-title":"Chembiochem"},{"key":"ref27","doi-asserted-by":"publisher","first-page":"27","DOI":"10.1186\/1756-3305-2-27","article-title":"In silico analysis of the cyclophilin repertoire of apicomplexan parasites","volume":"2","author":"Krucken","year":"2009","journal-title":"Parasit. Vectors"},{"key":"ref28","doi-asserted-by":"publisher","first-page":"161","DOI":"10.1007\/s12223-019-00717-z","article-title":"Comparative evaluation of structure and characteristic of peptidyl-prolyl cis-trans isomerase proteins and their function in Salmonella Typhimurium stress responses and virulence","volume":"65","author":"Kumawat","year":"2020","journal-title":"Folia Microbiol. (Praha)"},{"key":"ref29","doi-asserted-by":"publisher","first-page":"2304","DOI":"10.1073\/pnas.87.6.2304","article-title":"Cloning, expression, and purification of human cyclophilin in Escherichia coli and assessment of the catalytic role of cysteines by site-directed mutagenesis","volume":"87","author":"Liu","year":"1990","journal-title":"Proc. Natl. Acad. Sci. U. S. A."},{"key":"ref30","doi-asserted-by":"publisher","first-page":"2306","DOI":"10.1021\/bi00223a003","article-title":"Human and Escherichia coli cyclophilins: sensitivity to inhibition by the immunosuppressant cyclosporin A correlates with a specific tryptophan residue","volume":"30","author":"Liu","year":"1991","journal-title":"Biochemistry"},{"key":"ref31","doi-asserted-by":"publisher","first-page":"4028","DOI":"10.1073\/pnas.87.11.4028","article-title":"Peptidyl-prolyl cis-trans-isomerase from Escherichia coli: a periplasmic homolog of cyclophilin that is not inhibited by cyclosporin A","volume":"87","author":"Liu","year":"1990","journal-title":"Proc. Natl. Acad. Sci. U. S. A."},{"key":"ref32","doi-asserted-by":"publisher","first-page":"1261","DOI":"10.1111\/j.1462-5822.2011.01618.x","article-title":"In search of Brucella abortus type IV secretion substrates: screening and identification of four proteins translocated into host cells through VirB system","volume":"13","author":"Marchesini","year":"2011","journal-title":"Cell. Microbiol."},{"key":"ref33","doi-asserted-by":"publisher","first-page":"4625","DOI":"10.3390\/ijms12074625","article-title":"Acid-denatured green fluorescent protein (GFP) as model substrate to study the chaperone activity of protein disulfide isomerase","volume":"12","author":"Mares","year":"2011","journal-title":"Int. J. Mol. Sci."},{"key":"ref34","doi-asserted-by":"publisher","first-page":"5410","DOI":"10.1038\/s41598-018-23821-5","article-title":"Structural basis of interaction between dimeric cyclophilin 1 and Myb1 transcription factor in trichomonas vaginalis","volume":"8","author":"Martin","year":"2018","journal-title":"Sci. Rep."},{"key":"ref35","doi-asserted-by":"publisher","first-page":"339","DOI":"10.1186\/1471-2105-7-339","article-title":"Tools for integrated sequence-structure analysis with UCSF chimera","volume":"7","author":"Meng","year":"2006","journal-title":"BMC Bioinformatics"},{"key":"ref36","doi-asserted-by":"publisher","first-page":"31848","DOI":"10.1074\/jbc.M304258200","article-title":"Chloroplast cyclophilin is a target protein of thioredoxin. Thiol modulation of the peptidyl-prolyl cis-trans isomerase activity","volume":"278","author":"Motohashi","year":"2003","journal-title":"J. Biol. Chem."},{"key":"ref37","doi-asserted-by":"publisher","first-page":"321","DOI":"10.1042\/BJ20110767","article-title":"Varying dependency of periplasmic peptidylprolyl cis-trans isomerases in promoting Yersinia pseudotuberculosis stress tolerance and pathogenicity","volume":"439","author":"Obi","year":"2011","journal-title":"Biochem. J."},{"key":"ref38","doi-asserted-by":"publisher","first-page":"e0150288","DOI":"10.1371\/journal.pone.0150288","article-title":"Mycobacterium tuberculosis peptidyl-prolyl isomerases also exhibit chaperone like activity in-vitro and in-vivo","volume":"11","author":"Pandey","year":"2016","journal-title":"PLoS One"},{"key":"ref39","doi-asserted-by":"publisher","first-page":"38","DOI":"10.3389\/fcimb.2017.00038","article-title":"Mycobacterium tuberculosis peptidyl-prolyl isomerases are immunogenic, alter cytokine profile and aid in intracellular survival","volume":"7","author":"Pandey","year":"2017","journal-title":"Front. Cell. Infect. Microbiol."},{"key":"ref40","doi-asserted-by":"publisher","first-page":"244","DOI":"10.1186\/1471-2164-7-244","article-title":"Identification and comparative analysis of sixteen fungal peptidyl-prolyl cis\/trans isomerase repertoires","volume":"7","author":"Pemberton","year":"2006","journal-title":"BMC Genomics"},{"key":"ref41","doi-asserted-by":"publisher","first-page":"273","DOI":"10.1016\/j.ijbiomac.2016.02.070","article-title":"Determining the roles of a conserved tyrosine residue in a Mip-like peptidyl-prolyl cis-trans isomerase","volume":"87","author":"Polley","year":"2016","journal-title":"Int. J. Biol. Macromol."},{"key":"ref42","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.00939-17","article-title":"Peptidyl-prolyl-cis\/trans-isomerases Mip and PpiB of legionella pneumophila contribute to surface translocation, growth at suboptimal temperature, and infection","volume":"87","author":"Rasch","year":"2019","journal-title":"Infect. Immun."},{"key":"ref43","doi-asserted-by":"publisher","first-page":"1728","DOI":"10.1128\/IAI.06251-11","article-title":"Involvement of peptidylprolyl cis\/trans isomerases in Enterococcus faecalis virulence","volume":"80","author":"Reffuveille","year":"2012","journal-title":"Infect. Immun."},{"key":"ref44","doi-asserted-by":"publisher","first-page":"10637","DOI":"10.1038\/s41598-017-11283-0","article-title":"Iron-dependent reconfiguration of the proteome underlies the intracellular lifestyle of Brucella abortus","volume":"7","author":"Roset","year":"2017","journal-title":"Sci. Rep."},{"key":"ref45","doi-asserted-by":"publisher","first-page":"521","DOI":"10.1128\/IAI.01125-12","article-title":"Intracellularly induced cyclophilins play an important role in stress adaptation and virulence of Brucella abortus","volume":"81","author":"Roset","year":"2013","journal-title":"Infect. Immun."},{"key":"ref46","doi-asserted-by":"publisher","first-page":"509380","DOI":"10.3389\/fmicb.2020.509380","article-title":"Development of an antigen delivery platform using Lactobacillus acidophilus decorated with heterologous proteins: a sheep in Wolf's clothing story","volume":"11","author":"Uriza","year":"2020","journal-title":"Front. Microbiol."},{"key":"ref47","doi-asserted-by":"publisher","DOI":"10.1128\/JB.00453-16","article-title":"An intracellular peptidyl-prolyl cis\/trans isomerase is required for folding and activity of the Staphylococcus aureus secreted virulence factor nuclease","volume":"199","author":"Wiemels","year":"2017","journal-title":"J. Bacteriol."},{"key":"ref48","doi-asserted-by":"publisher","first-page":"666","DOI":"10.1016\/j.febslet.2013.01.028","article-title":"PPIase independent chaperone-like function of recombinant human Cyclophilin A during arginine kinase refolding","volume":"587","author":"Zhang","year":"2013","journal-title":"FEBS Lett."},{"key":"ref49","doi-asserted-by":"publisher","first-page":"1188","DOI":"10.2174\/092986611797642670","article-title":"Evidences of monomer, dimer and trimer of recombinant human cyclophilin A","volume":"18","author":"Zhang","year":"2011","journal-title":"Protein Pept. Lett."},{"key":"ref50","doi-asserted-by":"publisher","first-page":"1718","DOI":"10.1126\/science.279.5357.1718","article-title":"Activation of the OxyR transcription factor by reversible disulfide bond formation","volume":"279","author":"Zheng","year":"1998","journal-title":"Science"},{"key":"ref51","doi-asserted-by":"publisher","first-page":"1092","DOI":"10.1002\/pro.5560010903","article-title":"Active site mutants of human cyclophilin A separate peptidyl-prolyl isomerase activity from cyclosporin A binding and calcineurin inhibition","volume":"1","author":"Zydowsky","year":"1992","journal-title":"Protein Sci."}],"container-title":["Frontiers in Microbiology"],"original-title":[],"link":[{"URL":"https:\/\/www.frontiersin.org\/articles\/10.3389\/fmicb.2022.1046640\/full","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2022,10,31]],"date-time":"2022-10-31T04:22:57Z","timestamp":1667190177000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.frontiersin.org\/articles\/10.3389\/fmicb.2022.1046640\/full"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2022,10,31]]},"references-count":51,"alternative-id":["10.3389\/fmicb.2022.1046640"],"URL":"https:\/\/doi.org\/10.3389\/fmicb.2022.1046640","relation":{},"ISSN":["1664-302X"],"issn-type":[{"value":"1664-302X","type":"electronic"}],"subject":[],"published":{"date-parts":[[2022,10,31]]},"article-number":"1046640"}}