{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,24]],"date-time":"2026-09-24T05:28:42Z","timestamp":1790227722723,"version":"4.1.0"},"reference-count":47,"publisher":"Frontiers Media SA","license":[{"start":{"date-parts":[[2023,1,4]],"date-time":"2023-01-04T00:00:00Z","timestamp":1672790400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["frontiersin.org"],"crossmark-restriction":true},"short-container-title":["Front. Microbiol."],"abstract":"<jats:p>\n                    Antibiotics-resistant\n                    <jats:italic>Propionibacterium acne<\/jats:italic>\n                    (\n                    <jats:italic>P. acne<\/jats:italic>\n                    ) causes severe acne vulgaris, serious public health, and psychological threat. A new lytic bacteriophage (phage), \u03c6PaP11-13, infecting\n                    <jats:italic>P. acne,<\/jats:italic>\n                    was isolated from the sewage management center of Xinqiao Hospital. It can form transparent plaque with diameters of 1.0\u2009~\u20095.0\u2009mm on the double-layer agar plate, indicating a robust lytic ability against its host. Transmission electron microscopy (TEM) showed that \u03c6PaP11-13 belonged to the Siphoviridae family (head diameter 60\u2009\u00b1\u20094.5\u2009nm, tail length 170\u2009\u00b1\u20096.4\u2009nm, tail width 14\u2009\u00b1\u20092.4\u2009nm). The one-step growth curve showed the incubation period was 5\u2009h, and the burst size was 26 PFU (plaque-forming unit)\/cell. Moreover, it exhibited tolerance over a broad range of pH and temperature ranges but was utterly inactivated by ultraviolet (UV) irradiation for 1\u2009h. The whole-genome sequencing results revealed \u03c6PaP11-13 had a linear dsDNA with 29,648\u2009bp length. The G\/C content was 54.08%. Non-coding RNA genes and virulence factors were not found. Forty five open reading frames (ORFs) were identified after online annotation. This study reports a novel\n                    <jats:italic>P. acne<\/jats:italic>\n                    phage \u03c6PaP11-13, which has a robust lytic ability, no virulence factors, and good stability. The characterization and genomic analysis of \u03c6PaP11-13 will develop our understanding of phage biology and diversity and provide a potential arsenal for controlling antibiotics-resistant\n                    <jats:italic>P. acne<\/jats:italic>\n                    -induced severe acne vulgaris.\n                  <\/jats:p>","DOI":"10.3389\/fmicb.2022.1065386","type":"journal-article","created":{"date-parts":[[2023,1,4]],"date-time":"2023-01-04T14:07:00Z","timestamp":1672841220000},"update-policy":"https:\/\/doi.org\/10.3389\/crossmark-policy","source":"Crossref","is-referenced-by-count":13,"title":["Whole-genome sequencing, annotation, and biological characterization of a novel Siphoviridae phage against multi-drug resistant Propionibacterium acne"],"prefix":"10.3389","volume":"13","author":[{"given":"Danxi","family":"Liao","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jian","family":"Zhang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ruolan","family":"Liu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kui","family":"Chen","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yuanyuan","family":"Liu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yuming","family":"Shao","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xi","family":"Shi","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yiming","family":"Zhang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zichen","family":"Yang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1965","published-online":{"date-parts":[[2023,1,4]]},"reference":[{"key":"ref1","doi-asserted-by":"publisher","first-page":"W16","DOI":"10.1093\/nar\/gkw387","article-title":"PHASTER: a better, faster version of the PHAST phage search tool","volume":"44","author":"Arndt","year":"2016","journal-title":"Nucleic Acids Res."},{"key":"ref2","doi-asserted-by":"publisher","first-page":"75","DOI":"10.1186\/1471-2164-9-75","article-title":"The RAST server: rapid annotations using subsystems technology","volume":"9","author":"Aziz","year":"2008","journal-title":"BMC Genomics"},{"key":"ref3","doi-asserted-by":"publisher","first-page":"8365","DOI":"10.1038\/srep08365","article-title":"RASTtk: a modular and extensible implementation of the RAST algorithm for building custom annotation pipelines and annotating batches of genomes","volume":"5","author":"Brettin","year":"2015","journal-title":"Sci. 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