{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,19]],"date-time":"2026-01-19T05:50:34Z","timestamp":1768801834243,"version":"3.49.0"},"reference-count":61,"publisher":"Frontiers Media SA","license":[{"start":{"date-parts":[[2022,12,19]],"date-time":"2022-12-19T00:00:00Z","timestamp":1671408000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["frontiersin.org"],"crossmark-restriction":true},"short-container-title":["Front. Microbiol."],"abstract":"<jats:p>Confined environments increase psychological stress and lead to health problems such as abnormal mood and rhythm disruption. However, the mechanism by which confined environments impact health has remained unclear. Significant correlations have been reported between psychological stress and changes in gut microbiota. Therefore, we investigated the effect of a confined environment on the composition of the gut microbiota by 16s rDNA high-throughput sequencing, and analyzed the correlation between gut microbiota and health indicators such as uric acid (UA), sleep, and mood. We found that the gut microbiota of the subjects clustered into two enterotypes (Bi and Bla), and that the groups differed significantly. There were notable differences in the abundances of genera such as <jats:italic>Bifidobacterium<\/jats:italic>, <jats:italic>Dorea<\/jats:italic>, <jats:italic>Ruminococcus_torques_group<\/jats:italic>, <jats:italic>Ruminococcus_gnavus_group<\/jats:italic>, <jats:italic>Klebsiella<\/jats:italic>, and UCG-002 (<jats:italic>p<\/jats:italic>\u2009&amp;lt;\u20090.05). A confined environment significantly impacted the subjects\u2019 health indicators. We also observed differences in how the subjects of the two enterotypes adapted to the confined environment. The Bi group showed no significant differences in health indicators before and after confinement; however, the Bla group experienced several health problems after confinement, such as increased UA, anxiety, and constipation, and lack of sleep. Redundancy analysis (RDA) showed that UA, RBC, mood, and other health problems were significantly correlated with the structure of the gut microbiota. We concluded that genera such as UCG-002, <jats:italic>Ruminococcus<\/jats:italic>, CAG352, and <jats:italic>Ruminococcus_torques_group<\/jats:italic> increased vulnerability to confined environments, resulting in abnormal health conditions. We found that the differences in the adaptability of individuals to confined environments were closely related to the composition of their gut microbiota.<\/jats:p>","DOI":"10.3389\/fmicb.2022.1067756","type":"journal-article","created":{"date-parts":[[2022,12,19]],"date-time":"2022-12-19T04:35:34Z","timestamp":1671424534000},"update-policy":"https:\/\/doi.org\/10.3389\/crossmark-policy","source":"Crossref","is-referenced-by-count":13,"title":["The gut microbiota as a target to improve health conditions in a confined environment"],"prefix":"10.3389","volume":"13","author":[{"given":"Zheng","family":"Chen","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"ZiYing","family":"Wang","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Dan","family":"Li","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Beiwei","family":"Zhu","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yongjun","family":"Xia","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Guangqiang","family":"Wang","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Lianzhong","family":"Ai","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Chunhong","family":"Zhang","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Chuan","family":"Wang","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1965","published-online":{"date-parts":[[2022,12,19]]},"reference":[{"key":"ref1","doi-asserted-by":"publisher","first-page":"1","DOI":"10.1016\/j.bbi.2021.08.013","article-title":"Psychological stress disrupts intestinal epithelial cell function and mucosal integrity through microbe and host-directed processes","volume":"98","author":"Allen","year":"2021","journal-title":"Brain Behav. 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Metab."},{"key":"ref7","doi-asserted-by":"publisher","first-page":"44","DOI":"10.7205\/milmed.170.1.44","article-title":"Psychiatric symptoms and cognitive appraisal following the near sinking of a research submarine","volume":"170","author":"Berg","year":"2005","journal-title":"Mil. Med."},{"key":"ref8","doi-asserted-by":"publisher","first-page":"492","DOI":"10.3389\/FMICB.2016.00492","article-title":"Relationship between milk microbiota, bacterial load, macronutrients, and human cells during lactation","volume":"7","author":"Boix-Amor\u00f3s","year":"2016","journal-title":"Front. Microbiol."},{"key":"ref9","doi-asserted-by":"publisher","first-page":"535","DOI":"10.1097\/MCP.0000000000000329","article-title":"The development of lighting countermeasures for sleep disruption and circadian misalignment during spaceflight","volume":"22","author":"Brainard","year":"2016","journal-title":"Curr. Opin. Pulm. Med."},{"key":"ref10","doi-asserted-by":"publisher","first-page":"eaaw9969","DOI":"10.1126\/sciadv.aaw9969","article-title":"Ruminococcin C, a promising antibiotic produced by a human gut symbiont","volume":"5","author":"Chiumento","year":"2019","journal-title":"Sci. Adv."},{"key":"ref11","doi-asserted-by":"publisher","first-page":"66","DOI":"10.1038\/s41522-021-00235-2","article-title":"Metagenomic analysis revealed the potential role of gut microbiome in gout","volume":"7","author":"Chu","year":"2021","journal-title":"NPJ Biofilms Microbi."},{"key":"ref12","doi-asserted-by":"publisher","first-page":"1","DOI":"10.1080\/19490976.2022.2073784","article-title":"The role of the mucin-glycan foraging Ruminococcus gnavus in the communication between the gut and the brain","volume":"14","author":"Coletto","year":"2022","journal-title":"Gut Microbes"},{"key":"ref13","doi-asserted-by":"publisher","first-page":"545","DOI":"10.1016\/j.actaastro.2020.08.018","article-title":"Cognitive performance during prolonged periods in isolated, confined, and extreme environments","volume":"177","author":"Connaboy","year":"2020","journal-title":"Acta Astronaut."},{"key":"ref14","doi-asserted-by":"publisher","first-page":"2558","DOI":"10.3389\/fmicb.2018.02558","article-title":"Mechanistic insights into the cross-feeding of Ruminococcus gnavus and Ruminococcus bromii on host and dietary carbohydrates","volume":"9","author":"Crost","year":"2018","journal-title":"Front. 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Cachexia Sarcopeni."},{"key":"ref20","doi-asserted-by":"publisher","first-page":"211","DOI":"10.1016\/j.ijpsycho.2014.04.008","article-title":"How stressful are 105 days of isolation? Sleep EEG patterns and tonic cortisol in healthy volunteers simulating manned flight to Mars","volume":"93","author":"Gemignani","year":"2014","journal-title":"Int. J. Psychophysiol."},{"key":"ref21","doi-asserted-by":"publisher","first-page":"42","DOI":"10.1186\/2040-2392-4-42","article-title":"Increased abundance of Sutterella spp. and Ruminococcus torques in feces of children with autism spectrum disorder","volume":"4","author":"Gerber","year":"2013","journal-title":"Mol. Autism."},{"key":"ref22","doi-asserted-by":"publisher","first-page":"76","DOI":"10.1016\/j.sleep.2020.04.013","article-title":"Self-reported sleep quality is associated with gut microbiome composition in young, healthy individuals: a pilot study","volume":"73","author":"Grosicki","year":"2020","journal-title":"Sleep Med."},{"key":"ref23","doi-asserted-by":"publisher","first-page":"20602","DOI":"10.1038\/srep20602","article-title":"Intestinal microbiota distinguish gout patients from healthy humans","volume":"6","author":"Guo","year":"2016","journal-title":"Sci. Rep."},{"key":"ref24","doi-asserted-by":"publisher","first-page":"521","DOI":"10.22092\/ari.2020.352271.1557","article-title":"Bifidobacteriaceae family diversity in gut microbiota of patients with renal failure","volume":"76","author":"Hanifi","year":"2020","journal-title":"Arch Razi Inst."},{"key":"ref25","doi-asserted-by":"publisher","first-page":"e2007595118","DOI":"10.1073\/pnas.2007595118","article-title":"Capsular polysaccharide correlates with immune response to the human gut microbe Ruminococcus gnavus","volume":"118","author":"Henke","year":"2021","journal-title":"Proc. Natl. Acad. Sci. U. S. A."},{"key":"ref26","doi-asserted-by":"publisher","first-page":"441","DOI":"10.1017\/S0950268808001027","article-title":"Gastrointestinal illness associated with a long-haul flight","volume":"137","author":"Holmes","year":"2009","journal-title":"Epidemiol. Infect."},{"key":"ref27","doi-asserted-by":"publisher","first-page":"60","DOI":"10.1016\/j.anaerobe.2016.03.001","article-title":"Isolation and whole genome sequencing of a Ruminococcus-like bacterium, associated with irritable bowel syndrome","volume":"39","author":"Hyn\u00f6nen","year":"2016","journal-title":"Anaerobe"},{"key":"ref28","doi-asserted-by":"publisher","first-page":"413","DOI":"10.1016\/j.tins.2018.04.001","article-title":"Linking the gut microbiota to a brain neurotransmitter","volume":"41","author":"Jameson","year":"2018","journal-title":"Trends Neurosci."},{"key":"ref29","doi-asserted-by":"publisher","first-page":"3002","DOI":"10.1038\/s41467-022-30712-x","article-title":"The gut microbiota-bile acid axis links the positive association between chronic insomnia and cardiometabolic diseases","volume":"13","author":"Jiang","year":"2022","journal-title":"Nat. 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Pollut."},{"key":"ref33","doi-asserted-by":"publisher","first-page":"238","DOI":"10.1016\/j.actaastro.2020.06.034","article-title":"Differences in affordance-based behaviors within an isolated and confined environment are related to sleep, emotional health and physiological parameters","volume":"176","author":"LaGoy","year":"2020","journal-title":"Acta Astronaut."},{"key":"ref34","doi-asserted-by":"publisher","first-page":"13839","DOI":"10.1073\/pnas.1922498117","article-title":"Longitudinal analysis reveals transition barriers between dominant ecological states in the gut microbiome","volume":"117","author":"Levy","year":"2020","journal-title":"Proc. Natl. Acad. Sci. U. S. A."},{"key":"ref35","doi-asserted-by":"publisher","first-page":"1357","DOI":"10.1016\/j.jalz.2019.07.002","article-title":"Mild cognitive impairment has similar alterations as Alzheimer's disease in gut microbiota","volume":"15","author":"Li","year":"2019","journal-title":"Alzheimers Dement."},{"key":"ref36","doi-asserted-by":"publisher","first-page":"1233","DOI":"10.1111\/nmo.12822","article-title":"Gut microbes in correlation with mood: case study in a closed experimental human life support system","volume":"28","author":"Li","year":"2016","journal-title":"Neurogastroenterol. Motil."},{"key":"ref37","doi-asserted-by":"publisher","first-page":"53","DOI":"10.1016\/j.buildenv.2017.12.038","article-title":"Human responses to high air temperature, relative humidity and carbon dioxide concentration in underground refuge chamber","volume":"131","author":"Li","year":"2018","journal-title":"Build. Environ."},{"key":"ref38","doi-asserted-by":"publisher","first-page":"50","DOI":"10.1186\/s10020-021-00311-5","article-title":"Taxonomic variations in the gut microbiome of gout patients with and without tophi might have a functional impact on urate metabolism","volume":"27","author":"M\u00e9ndez-Salazar","year":"2021","journal-title":"Mol. Med."},{"key":"ref39","doi-asserted-by":"publisher","first-page":"139334","DOI":"10.1016\/j.scitotenv.2020.139334","article-title":"Investigation on entraining and enhancing human circadian rhythm in closed environments using daylight-like LED mixed lighting","volume":"732","author":"Nie","year":"2020","journal-title":"Sci. Total Environ."},{"key":"ref40","doi-asserted-by":"publisher","first-page":"13059","DOI":"10.1038\/S41598-021-92438-Y","article-title":"The effects of dynamic daylight-like light on the rhythm, cognition, and mood of irregular shift workers in closed environment","volume":"11","author":"Nie","year":"2021","journal-title":"Sci. Rep."},{"key":"ref41","doi-asserted-by":"publisher","first-page":"630","DOI":"10.1038\/S41564-019-0658-4","article-title":"Klebsiella michiganensis transmission enhances resistance to Enterobacteriaceae gut invasion by nutrition competition","volume":"5","author":"Oliveira","year":"2020","journal-title":"Nat. Microbiol."},{"key":"ref42","doi-asserted-by":"publisher","first-page":"413","DOI":"10.1016\/j.neubiorev.2021.03.032","article-title":"Psychosocial issues in isolated and confined extreme environments","volume":"126","author":"Palinkas","year":"2021","journal-title":"Neurosci. Biobehav. Rev."},{"key":"ref43","doi-asserted-by":"publisher","first-page":"707","DOI":"10.1177\/0013916509336889","article-title":"Interpersonal behavior in an isolated and confined environment","volume":"42","author":"Paul","year":"2010","journal-title":"Environ. Behav."},{"key":"ref44","doi-asserted-by":"publisher","first-page":"D590","DOI":"10.1093\/nar\/gks1219","article-title":"The SILVA ribosomal RNA gene database project: improved data processing and web-based tools","volume":"41","author":"Quast","year":"2013","journal-title":"Nucleic Acids Res."},{"key":"ref45","doi-asserted-by":"publisher","first-page":"199","DOI":"10.1007\/s12275-018-8024-4","article-title":"The Ruminococci: key symbionts of the gut ecosystem","volume":"56","author":"Reau","year":"2018","journal-title":"J. Microbiol."},{"key":"ref46","doi-asserted-by":"publisher","first-page":"1114","DOI":"10.3357\/asem.2875.2010","article-title":"Hypercortisolism as a potential concern for submariners","volume":"81","author":"Reini","year":"2010","journal-title":"Aviat. Space Environ. Med."},{"key":"ref47","doi-asserted-by":"publisher","first-page":"487","DOI":"10.1016\/j.jare.2017.03.003","article-title":"Physiological functions and pathogenic potential of uric acid: a review","volume":"8","author":"Ridi","year":"2017","journal-title":"J. Adv. Res."},{"key":"ref48","doi-asserted-by":"publisher","first-page":"377","DOI":"10.1370\/afm.209","article-title":"Stress and coping in a week-long disabled submarine exercise","volume":"2","author":"Risberg","year":"2004","journal-title":"Aviat. Space Environ. Med."},{"key":"ref49","doi-asserted-by":"publisher","first-page":"281","DOI":"10.1007\/s11157-006-9000-8","article-title":"Human challenges in polar and space environments","volume":"5","author":"Sandal","year":"2006","journal-title":"Rev. Environ. Sci. Bio."},{"key":"ref50","doi-asserted-by":"publisher","first-page":"101943","DOI":"10.1016\/j.cpr.2020.101943","article-title":"The gut microbiota in anxiety and depression -a systematic review","volume":"83","author":"Simpson","year":"2021","journal-title":"Clin. Psychol. Rev."},{"key":"ref51","doi-asserted-by":"publisher","first-page":"e00923-20","DOI":"10.1128\/MRA.00923-20","article-title":"Draft genome sequences of klebsiella species isolated from the international space station","volume":"9","author":"Solomon","year":"2020","journal-title":"Microbio. Resour. Ann."},{"key":"ref52","doi-asserted-by":"publisher","first-page":"42","DOI":"10.1136\/gutjnl-2018-317855","article-title":"Infants born to mothers with IBD present with altered gut microbiome that transfers abnormalities of the adaptive immune system to germ-free mice","volume":"69","author":"Torres","year":"2020","journal-title":"Gut"},{"key":"ref53","doi-asserted-by":"publisher","first-page":"706","DOI":"10.1080\/19490976.2019.1701352","article-title":"Temporal and region-specific effects of sleep fragmentation on gut microbiota and intestinal morphology in Sprague Dawley rats","volume":"11","author":"Triplett","year":"2020","journal-title":"Gut Microbes"},{"key":"ref54","doi-asserted-by":"publisher","first-page":"1319","DOI":"10.2337\/dc19-2533","article-title":"Microbiota-related metabolites and the risk of type 2 diabetes","volume":"43","author":"Vangipurapu","year":"2020","journal-title":"Diabetes Care"},{"key":"ref55","doi-asserted-by":"publisher","first-page":"E2703","DOI":"10.1073\/pnas.1402342111","article-title":"Dietary history contributes to enterotype-like clustering and functional metagenomic content in the intestinal microbiome of wild mice","volume":"111","author":"Wang","year":"2014","journal-title":"Proc. Natl. Acad. Sci. U. S. A."},{"key":"ref56","doi-asserted-by":"publisher","first-page":"95","DOI":"10.1016\/j.freeradbiomed.2021.12.307","article-title":"Enterocyte synthesizes and secrets uric acid as antioxidant to protect against oxidative stress via the involvement of Nrf pathway","volume":"179","author":"Wang","year":"2022","journal-title":"Free Radic. Biol. Med."},{"key":"ref57","doi-asserted-by":"publisher","first-page":"2131","DOI":"10.1136\/gutjnl-2019-319766","article-title":"Aberrant gut microbiota alters host metabolome and impacts renal failure in humans and rodents","volume":"69","author":"Wang","year":"2020","journal-title":"Gut"},{"key":"ref58","doi-asserted-by":"publisher","first-page":"682","DOI":"10.1002\/art.42009","article-title":"Association between gut microbiota and elevated serum urate in two independent cohorts","volume":"74","author":"Wei","year":"2022","journal-title":"Arthritis Rheumatol."},{"key":"ref59","doi-asserted-by":"publisher","first-page":"105","DOI":"10.1126\/science.1208344","article-title":"Linking long-term dietary patterns with gut microbial enterotypes","volume":"334","author":"Wu","year":"2011","journal-title":"Science"},{"key":"ref60","doi-asserted-by":"publisher","first-page":"417","DOI":"10.1016\/j.immuni.2020.06.025","article-title":"The gut microbiome regulates psychological-stress-induced inflammation","volume":"53","author":"Xu","year":"2020","journal-title":"Immunity"},{"key":"ref61","doi-asserted-by":"publisher","first-page":"2","DOI":"10.1186\/s40168-018-0608-z","article-title":"Impact of early events and lifestyle on the gut microbiota and metabolic phenotypes in young school-age children","volume":"7","author":"Zhong","year":"2019","journal-title":"Microbiome"}],"container-title":["Frontiers in Microbiology"],"original-title":[],"link":[{"URL":"https:\/\/www.frontiersin.org\/articles\/10.3389\/fmicb.2022.1067756\/full","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2022,12,19]],"date-time":"2022-12-19T04:35:40Z","timestamp":1671424540000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.frontiersin.org\/articles\/10.3389\/fmicb.2022.1067756\/full"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2022,12,19]]},"references-count":61,"alternative-id":["10.3389\/fmicb.2022.1067756"],"URL":"https:\/\/doi.org\/10.3389\/fmicb.2022.1067756","relation":{},"ISSN":["1664-302X"],"issn-type":[{"value":"1664-302X","type":"electronic"}],"subject":[],"published":{"date-parts":[[2022,12,19]]},"article-number":"1067756"}}