{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,30]],"date-time":"2026-09-30T03:50:26Z","timestamp":1790740226713,"version":"4.1.0"},"reference-count":22,"publisher":"Frontiers Media SA","license":[{"start":{"date-parts":[[2023,1,5]],"date-time":"2023-01-05T00:00:00Z","timestamp":1672876800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100007937","name":"Peking University","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100007937","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100007937","name":"Peking University","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100007937","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100006190","name":"Research and Development","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100006190","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["frontiersin.org"],"crossmark-restriction":true},"short-container-title":["Front. Microbiol."],"abstract":"<jats:sec><jats:title>Background<\/jats:title><jats:p>Intra-abdominal candidiasis (IAC) is the predominant type of invasive candidiasis with high mortality in critically ill patients. This study aimed to investigate whether the polymerase chain reaction (PCR) assay for detecting <jats:italic>Candida<\/jats:italic> DNA in peritoneal fluids (PF) is useful in diagnosing and management of IAC in high-risk patients in intensive care unit (ICU).<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>A prospective single-center cohort study of surgical patients at high risk for IAC was conducted in the ICU. PF was collected from the abdominal drainage tubes (within 24\u2009h) or by percutaneous puncture. Direct PF smear microscopy, PF culture, blood culture, and serum (1\u20133)-\u03b2-D-glucan were performed in all patients. For <jats:italic>Candida<\/jats:italic> PCR assay, the ITS1\/ITS4 primers that targeted the ITS1-5.8\u2009s-ITS2 regions were used for PCR, and sequencing analysis was used to identify the pathogen at the species level. IAC was defined according to the 2013 European consensus criteria.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Among 83 patients at high risk for IAC, the IAC criteria were present in 17 (20.5%). The sensitivity and specificity of the <jats:italic>Candida<\/jats:italic> PCR assay were 64.7 and 89.4%, respectively, and the area under the receiver operating characteristic curve was 0.77 (95% CI: 0.63\u20130.91). In this cohort, the positive predictive value and negative predictive value were 90.8% (95% CI: 80.3\u201396.2%) and 61.1% (95% CI: 36.1\u201381.7%), respectively. Diagnostic consistency was moderate (kappa 0.529, <jats:italic>p<\/jats:italic>\u2009&amp;lt;\u20090.001) according to the 2013 European consensus criteria.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>Detection of <jats:italic>Candida<\/jats:italic> DNA in PF using PCR can be considered an adjunct to existing routine diagnostic tools which may optimize the diagnosis and antifungal treatment of IAC in high-risk patients in the ICU.<\/jats:p><\/jats:sec>","DOI":"10.3389\/fmicb.2022.1070688","type":"journal-article","created":{"date-parts":[[2023,1,5]],"date-time":"2023-01-05T12:46:26Z","timestamp":1672922786000},"update-policy":"https:\/\/doi.org\/10.3389\/crossmark-policy","source":"Crossref","is-referenced-by-count":9,"title":["Detection of Candida DNA in peritoneal fluids by PCR assay optimizing the diagnosis and treatment for intra-abdominal candidiasis in high-risk ICU patients: A prospective cohort study"],"prefix":"10.3389","volume":"13","author":[{"given":"Min","family":"Xie","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jin","family":"Shao","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhe","family":"Wan","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ting","family":"Yan","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sainan","family":"Zhu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shuangling","family":"Li","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jin","family":"Yu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1965","published-online":{"date-parts":[[2023,1,5]]},"reference":[{"key":"ref1","doi-asserted-by":"publisher","first-page":"491","DOI":"10.1186\/s13104-015-1458-4","article-title":"Epidemiology of invasive candidiasis in a surgical intensive care unit: an observational study","volume":"8","author":"Aguilar","year":"2015","journal-title":"BMC. 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Microbiol."},{"key":"ref3","doi-asserted-by":"publisher","first-page":"2092","DOI":"10.1007\/s00134-013-3109-3","article-title":"A research agenda on the management of intraabdominal candidiasis: results from a consensus of multinational experts","volume":"39","author":"Bassetti","year":"2013","journal-title":"Intensive Care Med."},{"key":"ref4","doi-asserted-by":"publisher","first-page":"1601","DOI":"10.1007\/s00134-015-3866-24","article-title":"A multicenter multinational study of abdominal candidiasis: epidemiology, outcomes and predictors of mortality","volume":"41","author":"Bassetti","year":"2015","journal-title":"Intensive Care Med."},{"key":"ref5","doi-asserted-by":"publisher","first-page":"1284","DOI":"10.1093\/cid\/cit006","article-title":"Finding the \"missing 50%\" of invasive candidiasis: how nonculture diagnostics will improve understanding of disease spectrum and transform patient care","volume":"56","author":"Clancy","year":"2013","journal-title":"Clin. Infect. 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Mycol."},{"key":"ref8","doi-asserted-by":"publisher","first-page":"161","DOI":"10.4161\/viru.26187","article-title":"Invasive candidiasis as a cause of sepsis in the critically ill patient","volume":"5","author":"Delaloye","year":"2014","journal-title":"Virulence"},{"key":"ref9","doi-asserted-by":"publisher","first-page":"789","DOI":"10.1093\/mmy\/myz123","article-title":"Roles of the multiplex real-time PCR assay and \u03b2-D-glucan in a high-risk population for intra-abdominal candidiasis (IAC)","volume":"58","author":"Fort\u00fan","year":"2020","journal-title":"Med. Mycol."},{"key":"ref10","doi-asserted-by":"publisher","first-page":"3134","DOI":"10.1093\/jac\/dku225","article-title":"Clinical validation of a multiplex real-time PCR assay for detection of invasive candidiasis in intensive care unit patients","volume":"69","author":"Fort\u00fan","year":"2014","journal-title":"J. Antimicrob. Chemother."},{"key":"ref11","doi-asserted-by":"publisher","first-page":"R49","DOI":"10.1186\/cc12569","article-title":"Prediction of invasive candidal infection in critically ill patients with severe acute pancreatitis","volume":"17","author":"Hall","year":"2013","journal-title":"Crit. Care"},{"key":"ref12","doi-asserted-by":"publisher","first-page":"95","DOI":"10.1007\/s10096-016-2775-9","article-title":"Association between source control and mortality in 258 patients with intra-abdominal candidiasis: a retrospective multi-centric analysis comparing intensive care versus surgical wards in Spain","volume":"36","author":"Lagunes","year":"2017","journal-title":"Eur. J. Clin. Microbiol. Infect. Dis."},{"key":"ref13","doi-asserted-by":"publisher","first-page":"149","DOI":"10.1186\/s13054-016-1324-3","article-title":"Contribution of Candida biomarkers and DNA detection for the diagnosis of invasive candidiasis in ICU patients with severe abdominal conditions","volume":"20","author":"Le\u00f3n","year":"2016","journal-title":"Crit. Care"},{"key":"ref14","doi-asserted-by":"publisher","first-page":"1612","DOI":"10.1097\/CCM.0b013e31819efac0","article-title":"Epidemiology, management, and risk factors for death of invasive Candida infections in critical care: a multicenter, prospective, observational study in France (2005-2006)","volume":"37","author":"Leroy","year":"2009","journal-title":"Crit. Care Med."},{"key":"ref15","doi-asserted-by":"publisher","first-page":"789","DOI":"10.1007\/s00134-019-05599-w","article-title":"ESICM\/ESCMID task force on practical management of invasive candidiasis in critically ill patients","volume":"45","author":"Martin-Loeches","year":"2019","journal-title":"Intensive Care Med."},{"key":"ref16","doi-asserted-by":"publisher","first-page":"1240","DOI":"10.1093\/cid\/cis200","article-title":"Performance of Candida real-time polymerase chain reaction, beta-D-glucan assay, and blood cultures in the diagnosis of invasive candidiasis","volume":"54","author":"Nguyen","year":"2012","journal-title":"Clin. Infect. Dis."},{"key":"ref17","doi-asserted-by":"publisher","first-page":"21","DOI":"10.18502\/cmm.5.1.533","article-title":"Molecular identification and antifungal susceptibility pattern of Candida species isolated from HIV infected patients with candisiasis","volume":"5","author":"Paul","year":"2019","journal-title":"Curr. Med. Mycol."},{"key":"ref18","doi-asserted-by":"publisher","first-page":"130","DOI":"10.1016\/j.riam.2016.12.001","article-title":"J\u00e1vea consensus guidelines for the treatment of Candida peritonitis and other intra-abdominal fungal infections in non-neutropenic critically ill adult patients","volume":"34","author":"Pem\u00e1n","year":"2017","journal-title":"Rev. Iberoam. Micol."},{"key":"ref19","doi-asserted-by":"publisher","first-page":"304","DOI":"10.1007\/s00134-017-4683-6","article-title":"Surviving sepsis campaign: international guidelines for Management of Sepsis and Septic Shock: 2016","volume":"43","author":"Rhodes","year":"2017","journal-title":"Intensive Care Med."},{"key":"ref20","doi-asserted-by":"publisher","first-page":"903","DOI":"10.1007\/s00134-009-1405-8","article-title":"Caspofungin for prevention of intra-abdominal candidiasis in high-risk surgical patients","volume":"35","author":"Senn","year":"2009","journal-title":"Intensive Care Med."},{"key":"ref21","doi-asserted-by":"publisher","first-page":"1107","DOI":"10.1007\/s15010-021-01640-7","article-title":"Deciphering the epidemiology of invasive candidiasis in the intensive care unit: is it possible?","volume":"49","author":"Soulountsi","year":"2021","journal-title":"Infection"},{"key":"ref22","doi-asserted-by":"publisher","first-page":"1459","DOI":"10.1007\/s00268-020-05380-x","article-title":"Appropriate source control and antifungal therapy are associated with improved survival in critically ill surgical patients with intra-abdominal candidiasis","volume":"44","author":"Yan","year":"2020","journal-title":"World J. 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