{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,18]],"date-time":"2026-10-18T11:45:00Z","timestamp":1792323900977,"version":"4.4.0"},"reference-count":55,"publisher":"Frontiers Media SA","license":[{"start":{"date-parts":[[2023,1,11]],"date-time":"2023-01-11T00:00:00Z","timestamp":1673395200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["frontiersin.org"],"crossmark-restriction":true},"short-container-title":["Front. Microbiol."],"abstract":"<jats:sec><jats:title>Introduction<\/jats:title><jats:p>25-Hydroxycholecalciferol (25OHD<jats:sub>3<\/jats:sub>) is the active metabolite of regular vitamin D<jats:sub>3<\/jats:sub><jats:italic>in vivo<\/jats:italic>, which has a stronger biological activity and is more easily absorbed by the intestine than regular vitamin D<jats:sub>3<\/jats:sub>. Our study aimed to detect the potential influences of 25OHD<jats:sub>3<\/jats:sub> on pork quality, antioxidant status, and intestinal microbiota of growing-finishing pigs receiving low-phosphorus (P) diet.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods and results<\/jats:title><jats:p>Forty pigs [initial body weight (BW): 49.42\u2009\u00b1\u20094.01\u2009kg] were allocated into two groups including low-P diet (CON group) and low-P diet supplemented with 50\u2009\u03bcg\/kg 25OHD<jats:sub>3<\/jats:sub> (25OHD<jats:sub>3<\/jats:sub> group). The whole experiment lasted for 88\u2009days, including phase 1 (day 1\u201328), phase 2 (day 29\u201360), and phase 3 (day 61\u201388). The results showed that 25OHD<jats:sub>3<\/jats:sub> supplementation tended to decrease feed conversion ratio in phase 3 and overall phase in comparison with the CON group. 25OHD<jats:sub>3<\/jats:sub> increased (<jats:italic>p<\/jats:italic> &amp;lt;\u20090.05) serum contents of superoxide dismutase (SOD) and glutathione peroxidase (GSH-Px), and decreased (<jats:italic>p<\/jats:italic> &amp;lt;\u20090.05) serum bone-specific alkaline phosphatase level. 25OHD<jats:sub>3<\/jats:sub> increased (<jats:italic>p<\/jats:italic>\u2009&amp;lt;\u20090.05) mucosal GSH-Px activity in the duodenum and ileum, and tended to increase redness value and the activities of total antioxidant capacity and SOD in <jats:italic>longissimus dorsi<\/jats:italic>. 25OHD<jats:sub>3<\/jats:sub> significantly upregulated the mRNA level of <jats:italic>copper\/zinc superoxide dismutase<\/jats:italic>, and tended to change the mRNA levels of <jats:italic>nuclear factor E2-related factor 2<\/jats:italic> and <jats:italic>kelch-like ECH-associated protein 1<\/jats:italic> in <jats:italic>longissimus dorsi<\/jats:italic>. Moreover, 25OHD<jats:sub>3<\/jats:sub> supplementation decreased (<jats:italic>p<\/jats:italic>\u2009&amp;lt;\u20090.05) n-6\/n-3 and iodine value in <jats:italic>longissimus dorsi<\/jats:italic>. For bone quality, 25OHD<jats:sub>3<\/jats:sub> supplementation increased (<jats:italic>p<\/jats:italic>\u2009&amp;lt;\u20090.05) calcium content, bone mineral content, and breaking strength in the metacarpal bones. Moreover, the colonic abundance of <jats:italic>Lactobacillus<\/jats:italic> was significantly higher in pigs fed with 25OHD<jats:sub>3<\/jats:sub>, and exhibited a positive association with serum antioxidant status, pork quality, and bone characteristics.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>Overall, the inclusion of 25OHD<jats:sub>3<\/jats:sub> in low P diet partly improved production performance, meat quality, antioxidant capacity, bone properties, and gut microbiota composition of growing-finishing pigs.<\/jats:p><\/jats:sec>","DOI":"10.3389\/fmicb.2022.1095509","type":"journal-article","created":{"date-parts":[[2023,1,11]],"date-time":"2023-01-11T05:54:22Z","timestamp":1673416462000},"update-policy":"https:\/\/doi.org\/10.3389\/crossmark-policy","source":"Crossref","is-referenced-by-count":15,"title":["Dietary 25-hydroxycholecalciferol modulates gut microbiota and improves the growth, meat quality, and antioxidant status of growing-finishing pigs"],"prefix":"10.3389","volume":"13","author":[{"given":"Lianhua","family":"Zhang","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shenfei","family":"Long","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hongliang","family":"Wang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiangshu","family":"Piao","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1965","published-online":{"date-parts":[[2023,1,11]]},"reference":[{"key":"ref1","volume-title":"Official Methods of Analysis","year":"2006"},{"key":"ref2","doi-asserted-by":"publisher","first-page":"151","DOI":"10.1016\/j.aninu.2017.08.010","article-title":"Implications of butyrate and its derivatives for gut health and animal production","volume":"4","author":"Bedford","year":"2018","journal-title":"Anim. 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