{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,17]],"date-time":"2026-07-17T15:27:14Z","timestamp":1784302034989,"version":"3.55.0"},"reference-count":37,"publisher":"Frontiers Media SA","license":[{"start":{"date-parts":[[2022,6,15]],"date-time":"2022-06-15T00:00:00Z","timestamp":1655251200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["frontiersin.org"],"crossmark-restriction":true},"short-container-title":["Front. Microbiol."],"abstract":"<jats:p>The improved quality of sour soybean milk by adding polypeptide from <jats:italic>Pleurotus eryngii<\/jats:italic> was investigated in this study, and the immunomodulatory effect of sour soybean milk fermented with polypeptides from <jats:italic>P. eryngii<\/jats:italic> was also evaluated in immunosuppressed mice induced by cyclophosphamide. Results showed the physicochemical property of sour soybean milk fermented with small-molecular-weight polypeptide (&amp;lt;3 kDa) were superior to the others including the decrease of pH, and increase of acidity, water-holding capacity and lactic acid bacteria count. The animal experiment demonstrated that sour soybean milk with polypeptide could effectively reverse the decreasing trend of thymus\/spleen index and hematological parameters, enhance murine immune functions including serum hemolysin and splenic lymphocyte proliferation, and inhibit oxidative stress. In addition, sour soybean milk fermented with polypeptide could increase the diversity of intestinal flora, and increase the abundances of <jats:italic>Firmicutes<\/jats:italic>, <jats:italic>Bacteroides<\/jats:italic>, and <jats:italic>Lactobacillus<\/jats:italic>. Taken together, it could provide a theoretical basis for developing an immunomodulatory agent or functional food additives with antioxidant activity.<\/jats:p>","DOI":"10.3389\/fmicb.2022.750039","type":"journal-article","created":{"date-parts":[[2022,6,15]],"date-time":"2022-06-15T08:05:27Z","timestamp":1655280327000},"update-policy":"https:\/\/doi.org\/10.3389\/crossmark-policy","source":"Crossref","is-referenced-by-count":9,"title":["Antioxidant and Immunostimulatory Activities of Fermented Sour Soybean Milk Added With Polypeptides From Pleurotus eryngii"],"prefix":"10.3389","volume":"13","author":[{"given":"Xinling","family":"Song","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ximin","family":"Xu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wei","family":"Chen","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1965","published-online":{"date-parts":[[2022,6,15]]},"reference":[{"key":"B1","doi-asserted-by":"publisher","first-page":"390","DOI":"10.1016\/j.lwt.2018.09.022","article-title":"Developing functional yogurt rich in bioactive peptides and gamma-aminobutyric acid related to cardiovascular health.","volume":"98","author":"Abd El-Fattah","year":"2018","journal-title":"LWT Food Sci. Technol."},{"key":"B2","doi-asserted-by":"publisher","first-page":"2431","DOI":"10.1039\/c6fo00057f","article-title":"Antioxidant activities of bambara groundnut (Vigna subterranea) protein hydrolysates and their membrane ultrafiltration fractions.","volume":"7","author":"Arise","year":"2016","journal-title":"Food Funct."},{"key":"B3","doi-asserted-by":"publisher","first-page":"214","DOI":"10.1007\/s00284-016-1051-7","article-title":"In vitro and in vivo assessment of Angiotensin-Converting Enzyme (ACE) inhibitory activity of fermented soybean milk by Lactobacillus casei strains.","volume":"73","author":"Bao","year":"2016","journal-title":"Curr. Microbiol."},{"key":"B4","doi-asserted-by":"publisher","first-page":"5377","DOI":"10.1007\/s13197-015-1731-5","article-title":"Bioactive peptides of animal origin: a review.","volume":"52","author":"Bhat","year":"2015","journal-title":"J. Food Sci. Technol. Mysore"},{"key":"B5","doi-asserted-by":"publisher","first-page":"358","DOI":"10.1016\/j.ecoenv.2017.02.003","article-title":"Immune responses in the aquatic gastropod Lymnaea stagnalis under short-term exposure to pharmaceuticals of concern for immune systems: Diclofenac, cyclophosphamide and cyclosporine A.","volume":"139","author":"Boisseaux","year":"2017","journal-title":"Ecotoxicol. Environ. Saf."},{"key":"B6","doi-asserted-by":"crossref","first-page":"676","DOI":"10.1016\/j.tifs.2021.04.039","article-title":"An update on the nutritional, functional, sensory characteristics of soy products, and applications of new processing strategies.","volume":"112","author":"Cai","year":"2021","journal-title":"Trends Food Sci. Technol."},{"key":"B7","doi-asserted-by":"publisher","first-page":"362","DOI":"10.5851\/kosfa.2014.34.3.362","article-title":"Antioxidant effect and functional properties of hydrolysates derived from egg-white protein.","volume":"34","author":"Cho","year":"2014","journal-title":"Korean J. Food Sci. Anim. Resour."},{"key":"B8","doi-asserted-by":"publisher","first-page":"13432","DOI":"10.3390\/molecules190913432","article-title":"Immunomodulatory activity and partial characterisation of polysaccharides from Momordica charantia.","volume":"19","author":"Deng","year":"2014","journal-title":"Molecules"},{"key":"B9","doi-asserted-by":"publisher","first-page":"1416","DOI":"10.1016\/j.fct.2011.03.034","article-title":"Inhibitory effect of Pleurotus eryngii extracts on the activities of allergic mediators in antigen-stimulated mast cells.","volume":"49","author":"Han","year":"2011","journal-title":"Food Chem. Toxicol."},{"key":"B10","doi-asserted-by":"crossref","first-page":"219","DOI":"10.1016\/j.jff.2012.10.008","article-title":"Antioxidant activities of enzymatic rapeseed protein hydrolysates and the membrane ultrafiltration fractions.","volume":"5","author":"He","year":"2013","journal-title":"J. Funct. Foods"},{"key":"B11","doi-asserted-by":"publisher","first-page":"3764","DOI":"10.1039\/c8fo00604k","article-title":"Purification, identification and functional characterization of an immunomodulatory protein from Pleurotus eryngii.","volume":"9","author":"Hu","year":"2018","journal-title":"Food Funct."},{"key":"B12","doi-asserted-by":"publisher","DOI":"10.1002\/mnfr.201801265","article-title":"Dietary intake of Pleurotus eryngii ameliorated dextran-sodium-sulfate-induced colitis in mice.","volume":"63","author":"Hu","year":"2019","journal-title":"Mol. Nutr. Food Res."},{"key":"B13","doi-asserted-by":"publisher","first-page":"7064","DOI":"10.1021\/acs.jafc.1c02294","article-title":"Sodium alginate modulates immunity, intestinal mucosal barrier function, and gut microbiota in cyclophosphamide-induced immunosuppressed BALB\/c mice.","volume":"69","author":"Huang","year":"2021","journal-title":"J. Agric. Food Chem."},{"key":"B14","doi-asserted-by":"publisher","DOI":"10.1016\/j.foodchem.2020.128199","article-title":"Enhancement of isoflavone aglycone, amino acid, and CLA contents in fermented soybean yogurts using different strains: screening of antioxidant and digestive enzyme inhibition properties.","volume":"340","author":"Hwang","year":"2021","journal-title":"Food Chem."},{"key":"B15","doi-asserted-by":"publisher","first-page":"3249","DOI":"10.21037\/apm-20-1492","article-title":"Ma-Nuo-Xi decoction has an immunostimulating effect in cyclophosphamide-immunosuppressed mice.","volume":"9","author":"Jie","year":"2020","journal-title":"Ann. Palliat. Med."},{"key":"B16","doi-asserted-by":"publisher","DOI":"10.1155\/2015\/839697","article-title":"Immunoregulation on mice of low immunity and effects on five kinds of human cancer cells of Panax japonicus polysaccharide.","volume":"2015","author":"Jie","year":"2015","journal-title":"Evid. Based Complement Alternat. Med."},{"key":"B17","doi-asserted-by":"publisher","first-page":"1155","DOI":"10.1007\/s10068-019-00560-5","article-title":"The fermentation characteristics of soy yogurt with different content of d-allulose and sucrose fermented by lactic acid bacteria from kimchi.","volume":"28","author":"Kim","year":"2019","journal-title":"Food Sci. Biotechnol."},{"key":"B18","doi-asserted-by":"publisher","first-page":"58","DOI":"10.1016\/j.foodchem.2017.03.030","article-title":"Antioxidant potential of edible mushroom (Agaricus bisporus) protein hydrolysates and their ultrafiltration fractions.","volume":"230","author":"Kimatu","year":"2017","journal-title":"Food Chem."},{"key":"B19","doi-asserted-by":"publisher","first-page":"34276","DOI":"10.1039\/d0ra06977a","article-title":"Effect of soybean milk fermented withLactobacillus plantarumHFY01 isolated from yak yogurt on weight loss and lipid reduction in mice with obesity induced by a high-fat diet.","volume":"10","author":"Li","year":"2020","journal-title":"RSC Adv."},{"key":"B20","doi-asserted-by":"publisher","first-page":"2068","DOI":"10.1002\/fsn3.1048","article-title":"Effects of Lactobacillus plantarum CQPC01-fermented soybean milk on activated carbon-induced constipation through its antioxidant activity in mice.","volume":"7","author":"Liu","year":"2019","journal-title":"Food Sci. Nutr."},{"key":"B21","doi-asserted-by":"publisher","first-page":"48913","DOI":"10.1039\/c7ra08957k","article-title":"Detoxifying effects of ultrafiltration fractions of Dendrobium aphyllum peptides on chemical and AAPH-induced oxidative stress.","volume":"7","author":"Liu","year":"2017","journal-title":"RSC Adv."},{"key":"B22","doi-asserted-by":"publisher","first-page":"3831","DOI":"10.1039\/d0fo02905j","article-title":"Inhibitory effects of beta-type glycosidic polysaccharide from Pleurotus eryngii on dextran sodium sulfate-induced colitis in mice.","volume":"12","author":"Ma","year":"2021","journal-title":"Food Funct."},{"key":"B23","doi-asserted-by":"publisher","first-page":"3262","DOI":"10.1039\/c9fo00482c","article-title":"Protective effects of polysaccharides from Cordyceps gunnii mycelia against cyclophosphamide-induced immunosuppression to TLR4\/TRAF6\/NF-kappaB signalling in BALB\/c mice.","volume":"10","author":"Meng","year":"2019","journal-title":"Food Funct."},{"key":"B24","doi-asserted-by":"publisher","first-page":"467","DOI":"10.1016\/j.foodchem.2015.05.014","article-title":"Purification and characterization of antioxidant peptides from enzymatically hydrolyzed chicken egg white.","volume":"188","author":"Nimalaratne","year":"2015","journal-title":"Food Chem."},{"key":"B25","doi-asserted-by":"publisher","first-page":"1645","DOI":"10.1007\/s42770-020-00369-z","article-title":"Soymilk fermentation: effect of cooling protocol on cell viability during storage and in vitro gastrointestinal stress.","volume":"51","author":"Piazentin","year":"2020","journal-title":"Braz. J. Microbiol."},{"key":"B26","doi-asserted-by":"publisher","first-page":"123","DOI":"10.1016\/j.carbpol.2015.11.051","article-title":"Chemical characterization of Pleurotus eryngii polysaccharide and its tumor-inhibitory effects against human hepatoblastoma HepG-2 cells.","volume":"138","author":"Ren","year":"2016","journal-title":"Carbohydr. Polym."},{"key":"B27","doi-asserted-by":"publisher","first-page":"577","DOI":"10.1016\/j.jgr.2018.07.004","article-title":"A comparative study on immune-stimulatory and antioxidant activities of various types of ginseng extracts in murine and rodent models.","volume":"42","author":"Saba","year":"2018","journal-title":"J. Ginseng Res."},{"key":"B28","doi-asserted-by":"publisher","first-page":"323","DOI":"10.1016\/j.ijbiomac.2017.01.043","article-title":"Antioxidant, antitumor and immunostimulatory activities of the polypeptide from Pleurotus eryngii mycelium.","volume":"97","author":"Sun","year":"2017","journal-title":"Int. J. Biol. Macromol."},{"key":"B29","doi-asserted-by":"publisher","first-page":"82","DOI":"10.1016\/j.intimp.2017.08.005","article-title":"Activity-guided isolation and structural identification of immunomodulating substances from Pleurotus eryngii byproducts.","volume":"51","author":"Sun","year":"2017","journal-title":"Int. Immunopharmacol."},{"key":"B30","doi-asserted-by":"publisher","first-page":"199","DOI":"10.1007\/s11033-010-0095-1","article-title":"Experimental investigation of the immunoregulatory and anti-inflammatory effects of the traditional Chinese medicine \u201cLi-Yan Zhi-Ke Granule\u201d for relieving chronic pharyngitis in rats.","volume":"38","author":"Sun","year":"2011","journal-title":"Mol. Biol. Rep."},{"key":"B31","doi-asserted-by":"publisher","first-page":"2529","DOI":"10.1039\/c4fo00408f","article-title":"Dietary squid ink polysaccharides ameliorated the intestinal microflora dysfunction in mice undergoing chemotherapy.","volume":"5","author":"Tang","year":"2014","journal-title":"Food Funct."},{"key":"B32","doi-asserted-by":"publisher","first-page":"1","DOI":"10.1016\/j.peptides.2003.11.014","article-title":"Eryngin, a novel antifungal peptide from fruiting bodies of the edible mushroom Pleurotus eryngii.","volume":"25","author":"Wang","year":"2004","journal-title":"Peptides"},{"key":"B33","doi-asserted-by":"publisher","first-page":"951","DOI":"10.1016\/j.foodchem.2015.08.101","article-title":"Purification and identification of antioxidative peptides from dry-cured Xuanwei ham.","volume":"194","author":"Xing","year":"2016","journal-title":"Food Chem."},{"key":"B34","doi-asserted-by":"publisher","first-page":"1208","DOI":"10.1089\/jmf.2019.4467","article-title":"Lactobacillus plantarum CQPC02-fermented soybean milk improves loperamide-induced constipation in mice.","volume":"22","author":"Yi","year":"2019","journal-title":"J. Med. Food"},{"key":"B35","doi-asserted-by":"publisher","first-page":"2175","DOI":"10.1039\/c7fo00244k","article-title":"Isolation of a novel bioactive protein from an edible mushroom Pleurotus eryngii and its anti-inflammatory potential.","volume":"8","author":"Yuan","year":"2017","journal-title":"Food Funct."},{"key":"B36","doi-asserted-by":"publisher","first-page":"266","DOI":"10.1016\/j.foodchem.2019.01.168","article-title":"Purification and identification of an antioxidative peptide from peony (Paeonia suffruticosa Andr.) seed dreg.","volume":"285","author":"Zhang","year":"2019","journal-title":"Food Chem."},{"key":"B37","doi-asserted-by":"publisher","first-page":"7954","DOI":"10.1039\/D1FO01257F","article-title":"The immunoenhancement effects of sea buckthorn pulp oil in cyclophosphamide-induced immunosuppressed mice.","volume":"12","author":"Zhang","year":"2021","journal-title":"Food Funct."}],"container-title":["Frontiers in Microbiology"],"original-title":[],"link":[{"URL":"https:\/\/www.frontiersin.org\/articles\/10.3389\/fmicb.2022.750039\/full","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2022,6,15]],"date-time":"2022-06-15T08:05:32Z","timestamp":1655280332000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.frontiersin.org\/articles\/10.3389\/fmicb.2022.750039\/full"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2022,6,15]]},"references-count":37,"alternative-id":["10.3389\/fmicb.2022.750039"],"URL":"https:\/\/doi.org\/10.3389\/fmicb.2022.750039","relation":{},"ISSN":["1664-302X"],"issn-type":[{"value":"1664-302X","type":"electronic"}],"subject":[],"published":{"date-parts":[[2022,6,15]]},"article-number":"750039"}}