{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,29]],"date-time":"2026-09-29T06:48:17Z","timestamp":1790664497456,"version":"4.1.0"},"reference-count":112,"publisher":"Frontiers Media SA","license":[{"start":{"date-parts":[[2022,5,25]],"date-time":"2022-05-25T00:00:00Z","timestamp":1653436800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["frontiersin.org"],"crossmark-restriction":true},"short-container-title":["Front. Microbiol."],"abstract":"<jats:p>Lactic acid bacteria (LAB) are Gram-positive bacteria which are considered for use as adjuvant therapeutics in management of various disease ailments, including obesity, irritable bowel syndrome, lactose intolerance and cancer. To investigate the possible use of <jats:italic>Lactococcus lactis<\/jats:italic> strains from our collection in treatment of gastrointestinal cancer, we tested them for the ability to arrest proliferation of human colorectal adenocarcinoma cells (Caco-2). Results of the BrdU assay showed that the anti-proliferative activity of <jats:italic>L. lactis<\/jats:italic> cells is strain-specific. We found that particularly, two strains, <jats:italic>L. lactis<\/jats:italic> IBB109 and <jats:italic>L. lactis<\/jats:italic> IBB417, exhibited the most potent inhibitory effect. Moreover, both strains triggered interleukin 18 gene expression, normally inhibited in Caco-2 (cancer) cells. To examine the probiotic potential of the two strains, we tested them for bile salts and acid tolerance, as well as adhesion properties. Both isolates exhibited probiotic potential\u2014they survived in the presence of 0.3% bile salts and tolerated exposure to low pH and osmotic stress. Notably, we found that <jats:italic>L. lactis<\/jats:italic> IBB417 displayed better adherence to mucus and Caco-2 cells than <jats:italic>L. lactis<\/jats:italic> IBB109. Additionally, by microdilution tests we confirmed that both strains are sensitive to all nine antibiotics of human and veterinary importance listed by the European Food Safety Authority. Finally, by <jats:italic>in silico<\/jats:italic> investigations of whole genome sequencing data, we revealed the genetic features of <jats:italic>L. lactis<\/jats:italic> IBB109 and <jats:italic>L. lactis<\/jats:italic> IBB417 that can be associated with functional (e.g., adhesion and carbohydrate metabolic genes) and safety (e.g., virulence and antibiotic resistance) aspects of the strains, confirming their health-promoting potential.<\/jats:p>","DOI":"10.3389\/fmicb.2022.822912","type":"journal-article","created":{"date-parts":[[2022,5,25]],"date-time":"2022-05-25T07:38:51Z","timestamp":1653464331000},"update-policy":"https:\/\/doi.org\/10.3389\/crossmark-policy","source":"Crossref","is-referenced-by-count":37,"title":["Health-Promoting Nature of Lactococcus lactis IBB109 and Lactococcus lactis IBB417 Strains Exhibiting Proliferation Inhibition and Stimulation of Interleukin-18 Expression in Colorectal Cancer Cells"],"prefix":"10.3389","volume":"13","author":[{"given":"Przemys\u0142aw","family":"Sa\u0142a\u0144ski","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Magdalena","family":"Kowalczyk","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jacek K.","family":"Bardowski","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Agnieszka K.","family":"Szczepankowska","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1965","published-online":{"date-parts":[[2022,5,25]]},"reference":[{"key":"ref1","doi-asserted-by":"publisher","first-page":"D517","DOI":"10.1093\/nar\/gkz935","article-title":"CARD 2020: antibiotic resistome surveillance with the comprehensive antibiotic resistance database","volume":"48","author":"Alcock","year":"2020","journal-title":"Narrative"},{"key":"ref2","doi-asserted-by":"publisher","first-page":"202","DOI":"10.1093\/gbe\/evv247","article-title":"Genomic and functional characterization of the unusual pLOCK0919 plasmid harboring the spaCBA pili cluster in Lactobacillus casei LOCK 0919","volume":"8","author":"Aleksandrzak-Piekarczyk","year":"2016","journal-title":"Genome Biol. 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