{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,26]],"date-time":"2026-10-26T03:36:11Z","timestamp":1792985771265,"version":"4.5.0"},"reference-count":38,"publisher":"Frontiers Media SA","license":[{"start":{"date-parts":[[2022,3,7]],"date-time":"2022-03-07T00:00:00Z","timestamp":1646611200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100003593","name":"Conselho Nacional de Desenvolvimento Cient\u00edfico e Tecnol\u00f3gico","doi-asserted-by":"publisher","award":["302317\/2017-1"],"award-info":[{"award-number":["302317\/2017-1"]}],"id":[{"id":"10.13039\/501100003593","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100004586","name":"Funda\u00e7\u00e3o Carlos Chagas Filho de Amparo \u00e0 Pesquisa do Estado do Rio de Janeiro","doi-asserted-by":"publisher","award":["E-26\/202.896\/2018"],"award-info":[{"award-number":["E-26\/202.896\/2018"]}],"id":[{"id":"10.13039\/501100004586","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["frontiersin.org"],"crossmark-restriction":true},"short-container-title":["Front. Microbiol."],"abstract":"<jats:p>The HIV-1 epidemic in the Amazonas state, as in most of Brazil, is dominated by subtype B. The state, nonetheless, is singular for its significant co-circulation of the variants B<jats:sub>CAR<\/jats:sub>, which can mostly be found in the Caribbean region, and B<jats:sub>PAN<\/jats:sub>, a clade that emerged in the United States and aggregates almost the totality of subtype B infections world-wide. The Amazonian HIV-1 epidemic provides a unique scenario to compare the epidemic potential of B<jats:sub>PAN<\/jats:sub> and B<jats:sub>CAR<\/jats:sub> clades spreading in the same population. To reconstruct the spatiotemporal dynamic and demographic history of both subtype B lineages circulating in Amazonas, we analyzed 1,272 HIV-1 <jats:italic>pol<\/jats:italic> sequences sampled in that state between 2009 and 2018. Our phylogeographic analyses revealed that while most B<jats:sub>CAR<\/jats:sub> infections resulted from a single successful founder event that took place in the Amazonas state around the late 1970s, most B<jats:sub>PAN<\/jats:sub> infections resulted from the expansion of multiple clusters seeded in the state since the late 1980s. Our data support the existence of at least four large clusters of the pandemic form in Amazonas, two of them nested in Brazil\u2019s largest known subtype B cluster (B<jats:sub>BR\u2013I<\/jats:sub>), and two others resulting from new introductions detected here. The reconstruction of the demographic history of the most prevalent B<jats:sub>PAN<\/jats:sub> (<jats:italic>n<\/jats:italic> = 4) and B<jats:sub>CAR<\/jats:sub> (<jats:italic>n<\/jats:italic> = 1) clades identified in Amazonas revealed that all clades displayed a continuous expansion [effective reproductive number (<jats:italic>R<\/jats:italic><jats:sub>e<\/jats:sub>) &amp;gt; 1] until most recent times. During the period of co-circulation from the late 1990s onward, the R<jats:sub>e<\/jats:sub> of Amazonian B<jats:sub>PAN<\/jats:sub> and B<jats:sub>CAR<\/jats:sub> clusters behaved quite alike, fluctuating between 2.0 and 3.0. These findings support that the B<jats:sub>CAR<\/jats:sub> and B<jats:sub>PAN<\/jats:sub> variants circulating in the Brazilian state of Amazonas displayed different evolutionary histories, but similar epidemic trajectories and transmissibility over the last two decades, which is consistent with the notion that both subtype B variants display comparable epidemic potential. Our findings also revealed that despite significant advances in the treatment of HIV infections in the Amazonas state, B<jats:sub>CAR<\/jats:sub> and B<jats:sub>PAN<\/jats:sub> variants continue to expand and show no signs of the epidemic stabilization observed in other parts of the country.<\/jats:p>","DOI":"10.3389\/fmicb.2022.835443","type":"journal-article","created":{"date-parts":[[2022,3,7]],"date-time":"2022-03-07T09:26:19Z","timestamp":1646645179000},"update-policy":"https:\/\/doi.org\/10.3389\/crossmark-policy","source":"Crossref","is-referenced-by-count":2,"title":["Dissemination Dynamics of HIV-1 Subtype B Pandemic and Non-pandemic Lineages Circulating in Amazonas, Brazil"],"prefix":"10.3389","volume":"13","author":[{"given":"Ighor","family":"Arantes","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tiago","family":"Gr\u00e4f","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Paula","family":"Andrade","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yury","family":"Oliveira Chaves","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Monick Lindenmeyer","family":"Guimar\u00e3es","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gonzalo","family":"Bello","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1965","published-online":{"date-parts":[[2022,3,7]]},"reference":[{"key":"B1","doi-asserted-by":"publisher","first-page":"539","DOI":"10.1080\/10635150600755453","article-title":"Approximate likelihood-ratio test for branches: a fast, accurate, and powerful alternative.","volume":"55","author":"Anisimova","year":"2006","journal-title":"Syst. 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